BLASTX nr result
ID: Coptis23_contig00008514
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00008514
(1709 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAO17067.1| glycolate oxidase [Zantedeschia aethiopica] 553 e-155
ref|NP_001241302.1| peroxisomal (S)-2-hydroxy-acid oxidase GLO1-... 549 e-154
ref|NP_188060.1| putative peroxisomal (S)-2-hydroxy-acid oxidase... 544 e-153
ref|XP_002519658.1| (S)-2-hydroxy-acid oxidase, putative [Ricinu... 543 e-153
gb|AAL16258.1|AF428328_1 AT3g14420/MOA2_2 [Arabidopsis thaliana] 542 e-153
>gb|AAO17067.1| glycolate oxidase [Zantedeschia aethiopica]
Length = 367
Score = 553 bits (1424), Expect(2) = e-155
Identities = 275/321 (85%), Positives = 299/321 (93%)
Frame = +3
Query: 345 MEITNVEEYEPLAKQRLPKMVFDYYASGAEDQWTLRENRNAFSRILFRPRILIDVSHIDL 524
MEITNV EYE +AK +LPKMV+DYYASGAEDQWTL+ENRNAFSRILFRPRILIDV+ ID+
Sbjct: 1 MEITNVSEYEAVAKDKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVTKIDM 60
Query: 525 STTVLGYKISMPIMISPTAMQKMAHPDGEYXXXXXXXXXXXXMVLSSWSTSSVEEVASTG 704
+TTVLGYKISMPIMI+PTAMQKMAH DGEY M LSSW+TSSVEEVASTG
Sbjct: 61 TTTVLGYKISMPIMIAPTAMQKMAHLDGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
Query: 705 PGLRFFQLYVYKNRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPHLTL 884
PG+RFFQLYVYK+RNVVAQLVRRAE+AGFKAIALTVDTPRLGRRE+DIKNRFTLPPHLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPHLTL 180
Query: 885 KNFEGLDLGKMEKTSDSGLASYVAGQIDRSLEWKDIQWLQTITKLPILVKGVMTAEDTRL 1064
KNFEGLDLGKM+K++DSGLASYVAGQIDRSL WKD++WLQTIT +PILVKGVMTAEDTRL
Sbjct: 181 KNFEGLDLGKMDKSNDSGLASYVAGQIDRSLSWKDVKWLQTITSMPILVKGVMTAEDTRL 240
Query: 1065 SIQHGAAGIIVSNHGARQLDYVPATISCLEEVVKAAQGRIPVFLDGGVRRGTDVFKALAL 1244
++Q GAAGIIVSNHGARQLDYVPATISCLEEVVKAAQGR+PVFLDGGVRRGTDVFKALAL
Sbjct: 241 AVQAGAAGIIVSNHGARQLDYVPATISCLEEVVKAAQGRVPVFLDGGVRRGTDVFKALAL 300
Query: 1245 GASGIFIGRPVLYSLAVEGEA 1307
GASGIFIGRPV++SLA EGEA
Sbjct: 301 GASGIFIGRPVVFSLAAEGEA 321
Score = 23.9 bits (50), Expect(2) = e-155
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1
Query: 1426 RNHIVTDSDPPRPVSRL 1476
RNHI+T+ D R SRL
Sbjct: 351 RNHILTEGDVHRTASRL 367
>ref|NP_001241302.1| peroxisomal (S)-2-hydroxy-acid oxidase GLO1-like [Glycine max]
gi|167961875|dbj|BAG09382.1| peroxisomal glycolate
oxidase [Glycine max]
Length = 371
Score = 549 bits (1415), Expect(2) = e-154
Identities = 273/321 (85%), Positives = 298/321 (92%)
Frame = +3
Query: 345 MEITNVEEYEPLAKQRLPKMVFDYYASGAEDQWTLRENRNAFSRILFRPRILIDVSHIDL 524
MEITNV EYE +AKQ+LPKMVFDYYASGAEDQWTL+ENRNAFSRILFRPRILIDVS ID+
Sbjct: 1 MEITNVSEYEAIAKQKLPKMVFDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKIDI 60
Query: 525 STTVLGYKISMPIMISPTAMQKMAHPDGEYXXXXXXXXXXXXMVLSSWSTSSVEEVASTG 704
+TTVLG+KISMPIM++PTAMQKMAHP+GEY M LSSW+TSSVEEVASTG
Sbjct: 61 TTTVLGFKISMPIMLAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
Query: 705 PGLRFFQLYVYKNRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPHLTL 884
PG+RFFQLYVYK+RNVVAQLVRRAE+AGFKAIALTVDTPRLGRREADIKNRFTLPP LTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
Query: 885 KNFEGLDLGKMEKTSDSGLASYVAGQIDRSLEWKDIQWLQTITKLPILVKGVMTAEDTRL 1064
KNFEGLDLGKM+K DSGLASYVAGQIDR+L WKD++WLQTITKLPILVKGV+TAEDTR+
Sbjct: 181 KNFEGLDLGKMDKADDSGLASYVAGQIDRTLSWKDVKWLQTITKLPILVKGVLTAEDTRI 240
Query: 1065 SIQHGAAGIIVSNHGARQLDYVPATISCLEEVVKAAQGRIPVFLDGGVRRGTDVFKALAL 1244
++Q GAAGIIVSNHGARQLDYVPATIS LEEVVKAA+GR+PVFLDGGVRRGTDVFKALAL
Sbjct: 241 AVQSGAAGIIVSNHGARQLDYVPATISALEEVVKAAEGRVPVFLDGGVRRGTDVFKALAL 300
Query: 1245 GASGIFIGRPVLYSLAVEGEA 1307
GASGIFIGRPV++SLA EGEA
Sbjct: 301 GASGIFIGRPVVFSLAAEGEA 321
Score = 25.0 bits (53), Expect(2) = e-154
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +1
Query: 1426 RNHIVTDSDPPRPVSR 1473
R+HIVTD D PR + R
Sbjct: 351 RDHIVTDWDQPRTIPR 366
>ref|NP_188060.1| putative peroxisomal (S)-2-hydroxy-acid oxidase 2 [Arabidopsis
thaliana] gi|30683149|ref|NP_850584.1| putative
peroxisomal (S)-2-hydroxy-acid oxidase 2 [Arabidopsis
thaliana] gi|297829994|ref|XP_002882879.1| hypothetical
protein ARALYDRAFT_478862 [Arabidopsis lyrata subsp.
lyrata] gi|13124262|sp|Q9LRR9.1|GLO1_ARATH RecName:
Full=Peroxisomal (S)-2-hydroxy-acid oxidase GLO1;
AltName: Full=Glycolate oxidase 1; Short=AtGLO1;
Short=GOX 1; AltName: Full=Short chain alpha-hydroxy acid
oxidase GLO1 gi|16226423|gb|AAL16164.1|AF428396_1
AT3g14420/MOA2_2 [Arabidopsis thaliana]
gi|11994212|dbj|BAB01334.1| glycolate oxidase
[Arabidopsis thaliana] gi|15450741|gb|AAK96642.1|
AT3g14420/MOA2_2 [Arabidopsis thaliana]
gi|18491119|gb|AAL69528.1| AT3g14420/MOA2_2 [Arabidopsis
thaliana] gi|297328719|gb|EFH59138.1| hypothetical
protein ARALYDRAFT_478862 [Arabidopsis lyrata subsp.
lyrata] gi|332641997|gb|AEE75518.1| putative peroxisomal
(S)-2-hydroxy-acid oxidase 2 [Arabidopsis thaliana]
gi|332641999|gb|AEE75520.1| putative peroxisomal
(S)-2-hydroxy-acid oxidase 2 [Arabidopsis thaliana]
Length = 367
Score = 544 bits (1401), Expect(2) = e-153
Identities = 270/321 (84%), Positives = 296/321 (92%)
Frame = +3
Query: 345 MEITNVEEYEPLAKQRLPKMVFDYYASGAEDQWTLRENRNAFSRILFRPRILIDVSHIDL 524
MEITNV EY+ +AKQ+LPKMV+DYYASGAEDQWTL+ENRNAF+RILFRPRILIDVS ID+
Sbjct: 1 MEITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDM 60
Query: 525 STTVLGYKISMPIMISPTAMQKMAHPDGEYXXXXXXXXXXXXMVLSSWSTSSVEEVASTG 704
+TTVLG+KISMPIM++PTAMQKMAHPDGEY M LSSW+TSSVEEVASTG
Sbjct: 61 TTTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
Query: 705 PGLRFFQLYVYKNRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPHLTL 884
PG+RFFQLYVYKNRNVV QLVRRAE+AGFKAIALTVDTPRLGRRE+DIKNRFTLPP+LTL
Sbjct: 121 PGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTL 180
Query: 885 KNFEGLDLGKMEKTSDSGLASYVAGQIDRSLEWKDIQWLQTITKLPILVKGVMTAEDTRL 1064
KNFEGLDLGKM++ +DSGLASYVAGQIDR+L WKD+QWLQTITKLPILVKGV+T ED R+
Sbjct: 181 KNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARI 240
Query: 1065 SIQHGAAGIIVSNHGARQLDYVPATISCLEEVVKAAQGRIPVFLDGGVRRGTDVFKALAL 1244
+IQ GAAGIIVSNHGARQLDYVPATIS LEEVVKA QGRIPVFLDGGVRRGTDVFKALAL
Sbjct: 241 AIQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALAL 300
Query: 1245 GASGIFIGRPVLYSLAVEGEA 1307
GASGIFIGRPV++SLA EGEA
Sbjct: 301 GASGIFIGRPVVFSLAAEGEA 321
Score = 27.3 bits (59), Expect(2) = e-153
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1
Query: 1426 RNHIVTDSDPPRPVSRL 1476
RNHI T+ D PRP +RL
Sbjct: 351 RNHITTEWDTPRPSARL 367
>ref|XP_002519658.1| (S)-2-hydroxy-acid oxidase, putative [Ricinus communis]
gi|223541075|gb|EEF42631.1| (S)-2-hydroxy-acid oxidase,
putative [Ricinus communis]
Length = 369
Score = 543 bits (1400), Expect(2) = e-153
Identities = 271/321 (84%), Positives = 298/321 (92%)
Frame = +3
Query: 345 MEITNVEEYEPLAKQRLPKMVFDYYASGAEDQWTLRENRNAFSRILFRPRILIDVSHIDL 524
MEITNV EYE +A+Q+LPKMV+DYYASGAEDQWTL+ENRNAFSRILFRPRILIDVS ID+
Sbjct: 1 MEITNVMEYEEIARQKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
Query: 525 STTVLGYKISMPIMISPTAMQKMAHPDGEYXXXXXXXXXXXXMVLSSWSTSSVEEVASTG 704
+T+VLG+KISMPIMI+PTAMQKMAHP+GEY M LSSW+TSSVEEVASTG
Sbjct: 61 TTSVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
Query: 705 PGLRFFQLYVYKNRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPHLTL 884
PG+RFFQLYVY++RNVVAQLVRRAE+AGFKAIALTVDTPRLGRREADIKNRFTLPP LTL
Sbjct: 121 PGIRFFQLYVYRDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
Query: 885 KNFEGLDLGKMEKTSDSGLASYVAGQIDRSLEWKDIQWLQTITKLPILVKGVMTAEDTRL 1064
KNFEGLDLGKM+K+ DSGL+SYVAGQIDR+L WKDI+WLQTIT LPILVKGV+TAEDTRL
Sbjct: 181 KNFEGLDLGKMDKSDDSGLSSYVAGQIDRTLSWKDIKWLQTITSLPILVKGVLTAEDTRL 240
Query: 1065 SIQHGAAGIIVSNHGARQLDYVPATISCLEEVVKAAQGRIPVFLDGGVRRGTDVFKALAL 1244
+IQ+GAAGIIVSNHGARQLDYVPATI LEEVVKAAQGR+PVFLDGGVRRGTDVFKALAL
Sbjct: 241 AIQNGAAGIIVSNHGARQLDYVPATIMALEEVVKAAQGRVPVFLDGGVRRGTDVFKALAL 300
Query: 1245 GASGIFIGRPVLYSLAVEGEA 1307
GASGIFIGRPV++SLA EGEA
Sbjct: 301 GASGIFIGRPVVFSLAAEGEA 321
Score = 26.6 bits (57), Expect(2) = e-153
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = +1
Query: 1426 RNHIVTDSDPPRPVS 1470
R+HIVTD D PRPV+
Sbjct: 351 RDHIVTDWDLPRPVA 365
>gb|AAL16258.1|AF428328_1 AT3g14420/MOA2_2 [Arabidopsis thaliana]
Length = 367
Score = 542 bits (1397), Expect(2) = e-153
Identities = 269/321 (83%), Positives = 295/321 (91%)
Frame = +3
Query: 345 MEITNVEEYEPLAKQRLPKMVFDYYASGAEDQWTLRENRNAFSRILFRPRILIDVSHIDL 524
MEITNV EY+ +AKQ+LPKMV+DYYASGAEDQWTL+ENRNAF+RILFRPRILID S ID+
Sbjct: 1 MEITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDASKIDM 60
Query: 525 STTVLGYKISMPIMISPTAMQKMAHPDGEYXXXXXXXXXXXXMVLSSWSTSSVEEVASTG 704
+TTVLG+KISMPIM++PTAMQKMAHPDGEY M LSSW+TSSVEEVASTG
Sbjct: 61 TTTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
Query: 705 PGLRFFQLYVYKNRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPHLTL 884
PG+RFFQLYVYKNRNVV QLVRRAE+AGFKAIALTVDTPRLGRRE+DIKNRFTLPP+LTL
Sbjct: 121 PGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTL 180
Query: 885 KNFEGLDLGKMEKTSDSGLASYVAGQIDRSLEWKDIQWLQTITKLPILVKGVMTAEDTRL 1064
KNFEGLDLGKM++ +DSGLASYVAGQIDR+L WKD+QWLQTITKLPILVKGV+T ED R+
Sbjct: 181 KNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARI 240
Query: 1065 SIQHGAAGIIVSNHGARQLDYVPATISCLEEVVKAAQGRIPVFLDGGVRRGTDVFKALAL 1244
+IQ GAAGIIVSNHGARQLDYVPATIS LEEVVKA QGRIPVFLDGGVRRGTDVFKALAL
Sbjct: 241 AIQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALAL 300
Query: 1245 GASGIFIGRPVLYSLAVEGEA 1307
GASGIFIGRPV++SLA EGEA
Sbjct: 301 GASGIFIGRPVVFSLAAEGEA 321
Score = 27.3 bits (59), Expect(2) = e-153
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1
Query: 1426 RNHIVTDSDPPRPVSRL 1476
RNHI T+ D PRP +RL
Sbjct: 351 RNHITTEWDTPRPSARL 367