BLASTX nr result
ID: Coptis23_contig00008272
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00008272
(1912 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002317431.1| predicted protein [Populus trichocarpa] gi|2... 1047 0.0
gb|AAZ05069.1| pyruvate decarboxylase [Citrus sinensis] 1046 0.0
ref|XP_002522545.1| pyruvate decarboxylase, putative [Ricinus co... 1040 0.0
sp|P51850.1|PDC1_PEA RecName: Full=Pyruvate decarboxylase isozym... 1040 0.0
ref|XP_002305755.1| predicted protein [Populus trichocarpa] gi|2... 1032 0.0
>ref|XP_002317431.1| predicted protein [Populus trichocarpa] gi|222860496|gb|EEE98043.1|
predicted protein [Populus trichocarpa]
Length = 593
Score = 1047 bits (2707), Expect = 0.0
Identities = 503/571 (88%), Positives = 537/571 (94%)
Frame = -3
Query: 1730 PIPGGPSNGTLGRHLARRLVQIGVKDVFSVPGDFNLTLLDHLIAEPELNLIGCCNELNAG 1551
P+P +GTLG HLARRLV+IGV+DVFSVPGDFNLTLLDHLIAEPELNLIGCCNELNAG
Sbjct: 23 PVPAHACSGTLGSHLARRLVEIGVRDVFSVPGDFNLTLLDHLIAEPELNLIGCCNELNAG 82
Query: 1550 YAADGYARSKGVGACVVTFTVGGLSVLNAIAGSNSENLPVICIVGGPNSNDYGTSRILHH 1371
YAADGYAR+KGVGACVVTFTVGGLSVLNAIAG+ SENLPVICIVGGPNSNDYGT+RILHH
Sbjct: 83 YAADGYARAKGVGACVVTFTVGGLSVLNAIAGACSENLPVICIVGGPNSNDYGTNRILHH 142
Query: 1370 TIGLPDFSQELRCFQQVTCAQAVVNNLEDAHELIDTAISTALKESKPVYISISCNLPALP 1191
TIGLPDFSQELRCFQ VTC QAVVNNL+DAHE IDTAISTALKESKP YISISCNLP +P
Sbjct: 143 TIGLPDFSQELRCFQTVTCIQAVVNNLDDAHEQIDTAISTALKESKPAYISISCNLPGIP 202
Query: 1190 HPTFAMEPVPYFLAPMTSNQMGLEAAVEATADFLNKAVKPVLVCGPKIRVANAHKAFMEL 1011
HPTFA +PVP+FLAP SN +GLEAAVEATA+FLNKAVKPV+V GP +RVA A KAF+E
Sbjct: 203 HPTFARDPVPFFLAPKVSNHLGLEAAVEATAEFLNKAVKPVIVGGPNLRVAKAQKAFLEF 262
Query: 1010 ADASGYPVAIMPSGKGMVPENHPHFIGTYWGAVSTSYCGEIVESADAYVFVGPIFNDYSS 831
ADASGYP+A+MPSGKG+VPE+HPHFIGTYWGAVST +CGEIVESADAYVFVGPIFNDYSS
Sbjct: 263 ADASGYPIAVMPSGKGLVPEHHPHFIGTYWGAVSTGFCGEIVESADAYVFVGPIFNDYSS 322
Query: 830 VGYSLLIKKEKLVIVQPNRVTIGNGPSLGWVFMADFLSALAKKIKKNTTAMENYHRIFVP 651
VGYSLLIKKEK VIVQPNRVTIGNGPSLGWVFM DFLSALAKK+KKN+TA+ENY RIFVP
Sbjct: 323 VGYSLLIKKEKAVIVQPNRVTIGNGPSLGWVFMTDFLSALAKKLKKNSTALENYRRIFVP 382
Query: 650 SGIALKREIDEPLRVNILFKHIQNMLSGDTAVIAETGDSWFNCQKLRLPENCGYEFQMQY 471
GI LKRE DEPLRVN+LFKHIQ++L GDTAVI+ETGDSWFNCQKLRLPENCGYEFQMQY
Sbjct: 383 PGIPLKREQDEPLRVNVLFKHIQDILGGDTAVISETGDSWFNCQKLRLPENCGYEFQMQY 442
Query: 470 GSIGWSVGATLGYAQAAKDKRVIACIGDGSFQVTAQDISTMIRCGQRTIIFLINNGGYTI 291
GSIGWSVGATLGYAQAA+DKRVIACIGDGSFQVTAQDISTMIRCGQRTIIFLINNGGYTI
Sbjct: 443 GSIGWSVGATLGYAQAARDKRVIACIGDGSFQVTAQDISTMIRCGQRTIIFLINNGGYTI 502
Query: 290 EVEIHDGPYNVIKNWDYTKLVDAIHNGEGKCWTTKVRTEEQLTEAIATATGDQKDSLCFI 111
EVEIHDGPYNVIKNWDYT LV+AIHNGEGKCWT KVRTE++LT AIATATG+QKDSLCFI
Sbjct: 503 EVEIHDGPYNVIKNWDYTGLVNAIHNGEGKCWTAKVRTEDELTAAIATATGEQKDSLCFI 562
Query: 110 EVIAHKDDTSKELLEWGSRVAHANGRPPNPQ 18
EV HKDDTSKELLEWGSRV+ ANGRPPNPQ
Sbjct: 563 EVFVHKDDTSKELLEWGSRVSAANGRPPNPQ 593
>gb|AAZ05069.1| pyruvate decarboxylase [Citrus sinensis]
Length = 589
Score = 1046 bits (2706), Expect = 0.0
Identities = 502/571 (87%), Positives = 542/571 (94%)
Frame = -3
Query: 1730 PIPGGPSNGTLGRHLARRLVQIGVKDVFSVPGDFNLTLLDHLIAEPELNLIGCCNELNAG 1551
P+ GG S GTLGRHLARRLV+IG KDVFSVPGDFNLTLLDHLIAEPELNL+GCCNELNAG
Sbjct: 19 PVRGGASVGTLGRHLARRLVEIGAKDVFSVPGDFNLTLLDHLIAEPELNLVGCCNELNAG 78
Query: 1550 YAADGYARSKGVGACVVTFTVGGLSVLNAIAGSNSENLPVICIVGGPNSNDYGTSRILHH 1371
YAADGYARS+GVGACVVTFTVGGLSVLNAIAG+ SENLPVICIVGGPNSNDYGT+RILHH
Sbjct: 79 YAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHH 138
Query: 1370 TIGLPDFSQELRCFQQVTCAQAVVNNLEDAHELIDTAISTALKESKPVYISISCNLPALP 1191
TIGLPDF+QELRCFQ +TC+QAVVNNL DAHELIDTAISTALKESKPVYISISCNLP +P
Sbjct: 139 TIGLPDFTQELRCFQAITCSQAVVNNLGDAHELIDTAISTALKESKPVYISISCNLPGIP 198
Query: 1190 HPTFAMEPVPYFLAPMTSNQMGLEAAVEATADFLNKAVKPVLVCGPKIRVANAHKAFMEL 1011
HPTFA +PVP+FLAP SNQ+GLEAAVEATADFLNKAVKPVLV GP IRVA A KAF+EL
Sbjct: 199 HPTFARDPVPFFLAPKVSNQLGLEAAVEATADFLNKAVKPVLVGGPNIRVAKAQKAFIEL 258
Query: 1010 ADASGYPVAIMPSGKGMVPENHPHFIGTYWGAVSTSYCGEIVESADAYVFVGPIFNDYSS 831
ADA+GYP+AIMPSGKG+VPE+HPHFIGTYWGAVS+S+CGEIVESADAYVFVGPIFNDYSS
Sbjct: 259 ADATGYPIAIMPSGKGLVPEHHPHFIGTYWGAVSSSFCGEIVESADAYVFVGPIFNDYSS 318
Query: 830 VGYSLLIKKEKLVIVQPNRVTIGNGPSLGWVFMADFLSALAKKIKKNTTAMENYHRIFVP 651
VGYSLLIKKEK +IVQP+RVT+GNGPSLGWVFMADFLSALAKK++KNTTA+ENY RI+VP
Sbjct: 319 VGYSLLIKKEKAIIVQPHRVTVGNGPSLGWVFMADFLSALAKKLRKNTTALENYRRIYVP 378
Query: 650 SGIALKREIDEPLRVNILFKHIQNMLSGDTAVIAETGDSWFNCQKLRLPENCGYEFQMQY 471
GI +KR +EPLRVN+LFKHIQ+MLSGDTAVIAETGDSWFNCQKLRLPENCGYEFQMQY
Sbjct: 379 PGIPVKRAQNEPLRVNVLFKHIQDMLSGDTAVIAETGDSWFNCQKLRLPENCGYEFQMQY 438
Query: 470 GSIGWSVGATLGYAQAAKDKRVIACIGDGSFQVTAQDISTMIRCGQRTIIFLINNGGYTI 291
GSIGWSVGATLGYAQAAKDKRVIACIGDGSFQVTAQ+ISTMIRCGQR+IIFLINNGGYTI
Sbjct: 439 GSIGWSVGATLGYAQAAKDKRVIACIGDGSFQVTAQEISTMIRCGQRSIIFLINNGGYTI 498
Query: 290 EVEIHDGPYNVIKNWDYTKLVDAIHNGEGKCWTTKVRTEEQLTEAIATATGDQKDSLCFI 111
EVEIHDGPYNVIKNWDYT LV+AIHNGEGKCWT KVR+E++LTEA+ ATG+QKDSLCFI
Sbjct: 499 EVEIHDGPYNVIKNWDYTGLVNAIHNGEGKCWTAKVRSEDELTEAMKAATGEQKDSLCFI 558
Query: 110 EVIAHKDDTSKELLEWGSRVAHANGRPPNPQ 18
EV HKDDTSKELLEWGSRV+ AN RPPNPQ
Sbjct: 559 EVFVHKDDTSKELLEWGSRVSAANSRPPNPQ 589
>ref|XP_002522545.1| pyruvate decarboxylase, putative [Ricinus communis]
gi|223538236|gb|EEF39845.1| pyruvate decarboxylase,
putative [Ricinus communis]
Length = 589
Score = 1040 bits (2689), Expect = 0.0
Identities = 500/579 (86%), Positives = 539/579 (93%)
Frame = -3
Query: 1754 THEPSILKPIPGGPSNGTLGRHLARRLVQIGVKDVFSVPGDFNLTLLDHLIAEPELNLIG 1575
+H SI P+ G +GTLG HLARRLV+IGVKDVFSVPGDFNLTLLDHLIAEPELNLIG
Sbjct: 11 SHPSSISPPVRGNACSGTLGGHLARRLVEIGVKDVFSVPGDFNLTLLDHLIAEPELNLIG 70
Query: 1574 CCNELNAGYAADGYARSKGVGACVVTFTVGGLSVLNAIAGSNSENLPVICIVGGPNSNDY 1395
CCNELNAGYAADGYARS+GVGACVVTFTVGGLSV+NAIAG+ SENLPVICIVGGPNSNDY
Sbjct: 71 CCNELNAGYAADGYARSRGVGACVVTFTVGGLSVINAIAGAYSENLPVICIVGGPNSNDY 130
Query: 1394 GTSRILHHTIGLPDFSQELRCFQQVTCAQAVVNNLEDAHELIDTAISTALKESKPVYISI 1215
GT+RILHHTIGLPDFSQELRCFQ VTC QAVVNNL DAHE IDTAISTALKE+KP YISI
Sbjct: 131 GTNRILHHTIGLPDFSQELRCFQPVTCIQAVVNNLNDAHEQIDTAISTALKENKPAYISI 190
Query: 1214 SCNLPALPHPTFAMEPVPYFLAPMTSNQMGLEAAVEATADFLNKAVKPVLVCGPKIRVAN 1035
SCNLP +PHPTF EPVP+FLAP SNQ+GLEAAVEATA+FLNKAVKPV+V GPK+R
Sbjct: 191 SCNLPGIPHPTFGREPVPFFLAPSVSNQLGLEAAVEATAEFLNKAVKPVIVGGPKLRAVK 250
Query: 1034 AHKAFMELADASGYPVAIMPSGKGMVPENHPHFIGTYWGAVSTSYCGEIVESADAYVFVG 855
A KAF+ELADASGYPVA+MPSGKG+VPE+HPHFIGTYWGAVSTS+CGEIVESADAY+FVG
Sbjct: 251 AQKAFLELADASGYPVAVMPSGKGLVPEHHPHFIGTYWGAVSTSFCGEIVESADAYIFVG 310
Query: 854 PIFNDYSSVGYSLLIKKEKLVIVQPNRVTIGNGPSLGWVFMADFLSALAKKIKKNTTAME 675
PIFNDYSSVGYSLLIKKEKL+ VQPNRVTIGNGPS GWVFMADFLSAL+KK+K+N+TAME
Sbjct: 311 PIFNDYSSVGYSLLIKKEKLITVQPNRVTIGNGPSFGWVFMADFLSALSKKLKRNSTAME 370
Query: 674 NYHRIFVPSGIALKREIDEPLRVNILFKHIQNMLSGDTAVIAETGDSWFNCQKLRLPENC 495
NY RIFVPSG+ LK E DEPLRVN+LFKHIQ +LSG+TAVIAETGDSWFNCQKL LPENC
Sbjct: 371 NYRRIFVPSGVPLKSEKDEPLRVNVLFKHIQAILSGETAVIAETGDSWFNCQKLHLPENC 430
Query: 494 GYEFQMQYGSIGWSVGATLGYAQAAKDKRVIACIGDGSFQVTAQDISTMIRCGQRTIIFL 315
GYEFQMQYGSIGWSVGATLGYAQAA++KRVIACIGDGSFQVTAQDISTMIR GQR+IIFL
Sbjct: 431 GYEFQMQYGSIGWSVGATLGYAQAARNKRVIACIGDGSFQVTAQDISTMIRSGQRSIIFL 490
Query: 314 INNGGYTIEVEIHDGPYNVIKNWDYTKLVDAIHNGEGKCWTTKVRTEEQLTEAIATATGD 135
INNGGYTIEVEIHDGPYNVIKNW+YT LV+AIHNGEGKCWT KV TEE+LTEAIATA G+
Sbjct: 491 INNGGYTIEVEIHDGPYNVIKNWNYTGLVNAIHNGEGKCWTAKVHTEEELTEAIATAMGE 550
Query: 134 QKDSLCFIEVIAHKDDTSKELLEWGSRVAHANGRPPNPQ 18
QKDSLCFIEV+ HKDDTSKELLEWGSRV+ ANGRPPNPQ
Sbjct: 551 QKDSLCFIEVLVHKDDTSKELLEWGSRVSSANGRPPNPQ 589
>sp|P51850.1|PDC1_PEA RecName: Full=Pyruvate decarboxylase isozyme 1; Short=PDC
gi|1177603|emb|CAA91444.1| pyruvate decarboxylase [Pisum
sativum]
Length = 593
Score = 1040 bits (2688), Expect = 0.0
Identities = 507/593 (85%), Positives = 542/593 (91%), Gaps = 2/593 (0%)
Frame = -3
Query: 1790 METQPQLNNTTKTHEPSILKPIPGGPSN--GTLGRHLARRLVQIGVKDVFSVPGDFNLTL 1617
MET+ + N + S IP PS+ GT+GRHLARRLV+IGV+DVFSVPGDFNLTL
Sbjct: 1 METETETPNGSTPCPTSAPSAIPLRPSSCDGTMGRHLARRLVEIGVRDVFSVPGDFNLTL 60
Query: 1616 LDHLIAEPELNLIGCCNELNAGYAADGYARSKGVGACVVTFTVGGLSVLNAIAGSNSENL 1437
LDHLIAEPELNL+GCCNELNAGYAADGY R+KGVGACVVTFTVGGLS+LNAIAG+ SENL
Sbjct: 61 LDHLIAEPELNLVGCCNELNAGYAADGYGRAKGVGACVVTFTVGGLSILNAIAGAYSENL 120
Query: 1436 PVICIVGGPNSNDYGTSRILHHTIGLPDFSQELRCFQQVTCAQAVVNNLEDAHELIDTAI 1257
PVICIVGGPNSNDYGT+RILHHTIGLPDFSQEL+CFQ +TC QAVVNNL+DAHELIDTAI
Sbjct: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFSQELQCFQTITCFQAVVNNLDDAHELIDTAI 180
Query: 1256 STALKESKPVYISISCNLPALPHPTFAMEPVPYFLAPMTSNQMGLEAAVEATADFLNKAV 1077
STALKESKPVYISI CNLPA+PHPTFA +PVP+FLAP SNQ GLEAAVE A FLNKAV
Sbjct: 181 STALKESKPVYISIGCNLPAIPHPTFARDPVPFFLAPRVSNQAGLEAAVEEAAAFLNKAV 240
Query: 1076 KPVLVCGPKIRVANAHKAFMELADASGYPVAIMPSGKGMVPENHPHFIGTYWGAVSTSYC 897
KPV+V GPK+RVA A KAFME A+ASGYP+A+MPSGKG+VPENHPHFIGTYWGAVSTSYC
Sbjct: 241 KPVIVGGPKLRVAKAQKAFMEFAEASGYPIAVMPSGKGLVPENHPHFIGTYWGAVSTSYC 300
Query: 896 GEIVESADAYVFVGPIFNDYSSVGYSLLIKKEKLVIVQPNRVTIGNGPSLGWVFMADFLS 717
GEIVESADAYVFVGPIFNDYSSVGYSLLIKKEK +IVQPNRVTIGNG SLGWVFMADFL+
Sbjct: 301 GEIVESADAYVFVGPIFNDYSSVGYSLLIKKEKSLIVQPNRVTIGNGLSLGWVFMADFLT 360
Query: 716 ALAKKIKKNTTAMENYHRIFVPSGIALKREIDEPLRVNILFKHIQNMLSGDTAVIAETGD 537
ALAKK+K NTTA+ENY RI+VP GI LKRE DEPLRVN+LFKHIQ ++SGDTAVIAETGD
Sbjct: 361 ALAKKVKTNTTAVENYRRIYVPPGIPLKREKDEPLRVNVLFKHIQALISGDTAVIAETGD 420
Query: 536 SWFNCQKLRLPENCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVIACIGDGSFQVTAQDI 357
SWFNCQKLRLPENCGYEFQMQYGSIGWSVGATLGYAQAA DKRVIACIGDGSFQVTAQDI
Sbjct: 421 SWFNCQKLRLPENCGYEFQMQYGSIGWSVGATLGYAQAATDKRVIACIGDGSFQVTAQDI 480
Query: 356 STMIRCGQRTIIFLINNGGYTIEVEIHDGPYNVIKNWDYTKLVDAIHNGEGKCWTTKVRT 177
STMIRCGQR+IIFLINNGGYTIEVEIHDGPYNVIKNWDYT V AIHNG+GKCWT KVRT
Sbjct: 481 STMIRCGQRSIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGFVSAIHNGQGKCWTAKVRT 540
Query: 176 EEQLTEAIATATGDQKDSLCFIEVIAHKDDTSKELLEWGSRVAHANGRPPNPQ 18
EE LTEAIATATG +KDSLCFIEV AHKDDTSKELLEWGSRVA AN RPPNPQ
Sbjct: 541 EEDLTEAIATATGAEKDSLCFIEVFAHKDDTSKELLEWGSRVAAANSRPPNPQ 593
>ref|XP_002305755.1| predicted protein [Populus trichocarpa] gi|222848719|gb|EEE86266.1|
predicted protein [Populus trichocarpa]
Length = 582
Score = 1032 bits (2669), Expect = 0.0
Identities = 496/571 (86%), Positives = 532/571 (93%)
Frame = -3
Query: 1730 PIPGGPSNGTLGRHLARRLVQIGVKDVFSVPGDFNLTLLDHLIAEPELNLIGCCNELNAG 1551
P+PG NGTLG HLARRLV+IGV DVFSVPGDFNLTLLDHLI EPELNLIGCCNELNAG
Sbjct: 12 PVPGHTFNGTLGHHLARRLVEIGVSDVFSVPGDFNLTLLDHLIDEPELNLIGCCNELNAG 71
Query: 1550 YAADGYARSKGVGACVVTFTVGGLSVLNAIAGSNSENLPVICIVGGPNSNDYGTSRILHH 1371
YAADGYAR+KGVGACVVTFTVGGLSVLNAIAG+ SENLP+ICIVGGPNSNDYGT+RILHH
Sbjct: 72 YAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPIICIVGGPNSNDYGTNRILHH 131
Query: 1370 TIGLPDFSQELRCFQQVTCAQAVVNNLEDAHELIDTAISTALKESKPVYISISCNLPALP 1191
T GLPDF+QELRCFQ VTC QAVV NL+DAHE IDTAISTALKESKP YISISCNL +P
Sbjct: 132 TTGLPDFTQELRCFQTVTCVQAVVTNLDDAHEQIDTAISTALKESKPAYISISCNLSGIP 191
Query: 1190 HPTFAMEPVPYFLAPMTSNQMGLEAAVEATADFLNKAVKPVLVCGPKIRVANAHKAFMEL 1011
HPTF+ EPVP+FLAP SN +GLEAAVEATA+FLNKAVKPV++ GPK+RVA A KAF+EL
Sbjct: 192 HPTFSREPVPFFLAPKVSNYLGLEAAVEATAEFLNKAVKPVIIGGPKLRVAKAQKAFIEL 251
Query: 1010 ADASGYPVAIMPSGKGMVPENHPHFIGTYWGAVSTSYCGEIVESADAYVFVGPIFNDYSS 831
ADASGYP+A+MPSGKG+VPE+HPHFIGTYWGAVSTS+C EIVESADAYVFVGPIFNDYSS
Sbjct: 252 ADASGYPIAVMPSGKGLVPEHHPHFIGTYWGAVSTSFCAEIVESADAYVFVGPIFNDYSS 311
Query: 830 VGYSLLIKKEKLVIVQPNRVTIGNGPSLGWVFMADFLSALAKKIKKNTTAMENYHRIFVP 651
VGYSLLIKKEK +IVQPNRVTIGNG SLGWVFMADFLSALAKK+KKN+TA+ENY RIFVP
Sbjct: 312 VGYSLLIKKEKSIIVQPNRVTIGNGLSLGWVFMADFLSALAKKLKKNSTALENYRRIFVP 371
Query: 650 SGIALKREIDEPLRVNILFKHIQNMLSGDTAVIAETGDSWFNCQKLRLPENCGYEFQMQY 471
G+ L RE DEPLRVN+LFKHIQNML GD+AVIAETGDSWFNCQKL LPENCGYEFQMQY
Sbjct: 372 PGMPLMREKDEPLRVNVLFKHIQNMLGGDSAVIAETGDSWFNCQKLCLPENCGYEFQMQY 431
Query: 470 GSIGWSVGATLGYAQAAKDKRVIACIGDGSFQVTAQDISTMIRCGQRTIIFLINNGGYTI 291
GSIGWSVGATLGYAQAA+DKRVIACIGDGSFQVTAQDISTMIRCGQRTIIFLINNGGYTI
Sbjct: 432 GSIGWSVGATLGYAQAARDKRVIACIGDGSFQVTAQDISTMIRCGQRTIIFLINNGGYTI 491
Query: 290 EVEIHDGPYNVIKNWDYTKLVDAIHNGEGKCWTTKVRTEEQLTEAIATATGDQKDSLCFI 111
EVEIHDGPYNVIKNWDYT LV+AIHNGEGKCWT KVRTE++LT AIATATG+QKDSLCFI
Sbjct: 492 EVEIHDGPYNVIKNWDYTGLVNAIHNGEGKCWTAKVRTEDELTAAIATATGEQKDSLCFI 551
Query: 110 EVIAHKDDTSKELLEWGSRVAHANGRPPNPQ 18
E+ HKDDTSKELLEWGSRV+ AN RPPNPQ
Sbjct: 552 EIFVHKDDTSKELLEWGSRVSAANSRPPNPQ 582