BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00006989
         (374 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI16383.3| unnamed protein product [Vitis vinifera]              108   3e-22
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   108   3e-22
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...    85   7e-15
ref|XP_002298404.1| predicted protein [Populus trichocarpa] gi|2...    82   5e-14
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...    79   3e-13

>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
          Length = 544

 Score =  108 bits (271), Expect = 3e-22
 Identities = 56/103 (54%), Positives = 76/103 (73%), Gaps = 7/103 (6%)
 Frame = -2

Query: 373 LGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE--PKGN-----IQLHK 215
           +GSG+ILESW+DQGFPVC +ALEIAR+EDKSKLGKPVKI V+PE  PKG+     I+L +
Sbjct: 444 IGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETPPKGSDQIDGIKLDR 503

Query: 214 EAAMAKVTPSEPVIETPRPASQGIFSSCFPMNWLKELRNKWLR 86
           E A ++   ++ +      +S+G   S FPMNWL+++R KWL+
Sbjct: 504 ELANSQAAVTDEISR----SSEGEARSRFPMNWLQKIREKWLQ 542


>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
          Length = 540

 Score =  108 bits (271), Expect = 3e-22
 Identities = 56/103 (54%), Positives = 76/103 (73%), Gaps = 7/103 (6%)
 Frame = -2

Query: 373 LGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE--PKGN-----IQLHK 215
           +GSG+ILESW+DQGFPVC +ALEIAR+EDKSKLGKPVKI V+PE  PKG+     I+L +
Sbjct: 440 IGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETPPKGSDQIDGIKLDR 499

Query: 214 EAAMAKVTPSEPVIETPRPASQGIFSSCFPMNWLKELRNKWLR 86
           E A ++   ++ +      +S+G   S FPMNWL+++R KWL+
Sbjct: 500 ELANSQAAVTDEISR----SSEGEARSRFPMNWLQKIREKWLQ 538


>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis
           sativus] gi|449524848|ref|XP_004169433.1| PREDICTED:
           tRNA-specific 2-thiouridylase MnmA-like [Cucumis
           sativus]
          Length = 538

 Score = 84.7 bits (208), Expect = 7e-15
 Identities = 46/105 (43%), Positives = 64/105 (60%), Gaps = 7/105 (6%)
 Frame = -2

Query: 373 LGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE-------PKGNIQLHK 215
           +GSG+ILESW+DQGFPVC +A+E A++EDKSKLGKP+KI V+PE        K + ++H 
Sbjct: 438 IGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPESGMKESKAKDDTEIHD 497

Query: 214 EAAMAKVTPSEPVIETPRPASQGIFSSCFPMNWLKELRNKWLRTF 80
           +    + T    V+E  R  S     +   M WL  L+ K L+ F
Sbjct: 498 KLRNEEAT----VVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538


>ref|XP_002298404.1| predicted protein [Populus trichocarpa] gi|222845662|gb|EEE83209.1|
           predicted protein [Populus trichocarpa]
          Length = 434

 Score = 82.0 bits (201), Expect = 5e-14
 Identities = 42/76 (55%), Positives = 54/76 (71%)
 Frame = -2

Query: 373 LGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPEPKGNIQLHKEAAMAKV 194
           +GSG+ILESW+D+GFPVC +ALE+ARLEDKSKLGKPVKI V+PE      + K+     +
Sbjct: 364 IGSGVILESWDDKGFPVCQKALELARLEDKSKLGKPVKIKVKPETSDQESVQKDG----I 419

Query: 193 TPSEPVIETPRPASQG 146
            P + + E PR A  G
Sbjct: 420 EPDKGLAE-PRVAVTG 434


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
           putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
           tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
           putative [Ricinus communis]
          Length = 497

 Score = 79.3 bits (194), Expect = 3e-13
 Identities = 35/44 (79%), Positives = 41/44 (93%)
 Frame = -2

Query: 373 LGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE 242
           +GSG+ILESW DQGFPVC RALE+AR+EDKS+LGKPVKI V+PE
Sbjct: 427 IGSGVILESWNDQGFPVCERALELARIEDKSELGKPVKIKVKPE 470