BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00005495
         (376 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf...   207   6e-52
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]        105   4e-21
gb|ACO90225.1| putative norcoclaurine 6-O-methyltransferase [Pap...    94   9e-18
emb|CAJ65596.1| putative orcinol O-methyltransferase [Rosa chine...    93   2e-17
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...    92   6e-17

>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT;
           AltName: Full=Tetrahydrocolumbamine
           2-O-methyltransferase gi|24210466|dbj|BAC22084.1|
           columbamine O-methyltransferase [Coptis japonica]
          Length = 351

 Score =  207 bits (528), Expect = 6e-52
 Identities = 101/117 (86%), Positives = 108/117 (92%), Gaps = 1/117 (0%)
 Frame = +2

Query: 29  MDTPKPYQND-EINAQAEVWKHMYGFAKTIMLRSTISLGIPDIIHNNGPVTLSQLATHLP 205
           MDTP  +QND EI AQA+VWKHM+GFA+TIMLRST+SLGIPDIIHNNGPVTLSQL THLP
Sbjct: 1   MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLP 60

Query: 206 LKSISIDRFHHLMRYLVHMKLFTISTDHITKEDKFELTPASKLLVQGHQKSLAPYVM 376
           LKS SIDRFHH MRYLVHM+LFTISTD ITKEDK+ELTPASKLLV GHQKSLAPYVM
Sbjct: 61  LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVM 117


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score =  105 bits (262), Expect = 4e-21
 Identities = 47/103 (45%), Positives = 75/103 (72%), Gaps = 1/103 (0%)
 Frame = +2

Query: 71  QAEVWKHMYGFAKTIMLRSTISLGIPDIIHNNG-PVTLSQLATHLPLKSISIDRFHHLMR 247
           QA++WKH+YGFA++++L+S + L I D IHN+G P+TLS+LA+ LP++ + +DR + +MR
Sbjct: 14  QAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDRLYRVMR 73

Query: 248 YLVHMKLFTISTDHITKEDKFELTPASKLLVQGHQKSLAPYVM 376
           YLVHMKLF    D   +  K+ L P +K L++G +KS+   ++
Sbjct: 74  YLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAIL 116


>gb|ACO90225.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
          Length = 357

 Score = 94.4 bits (233), Expect = 9e-18
 Identities = 49/118 (41%), Positives = 80/118 (67%), Gaps = 9/118 (7%)
 Frame = +2

Query: 50  QNDEINAQAEVWKHMYGFAKTIMLRSTISLGIPDIIHNNG-PVTLSQLATHLPL-KSISI 223
           Q D+ N QA +WK +YGFA++++L+  + L I + IHN+G P++LS+LA+ LP+ + ++I
Sbjct: 6   QTDQEN-QAIIWKQIYGFAESLVLKCAVQLEIAETIHNHGKPMSLSELASKLPIDQPVNI 64

Query: 224 DRFHHLMRYLVHMKLFTISTDHITK-------EDKFELTPASKLLVQGHQKSLAPYVM 376
           DR + +MR+LVHMKLF      I         EDK+ L P +K L++G ++S+ P+V+
Sbjct: 65  DRLYRVMRFLVHMKLFNKENISILSGETAQVVEDKYSLAPPAKYLIRGSEQSMVPWVL 122


>emb|CAJ65596.1| putative orcinol O-methyltransferase [Rosa chinensis]
          Length = 345

 Score = 93.2 bits (230), Expect = 2e-17
 Identities = 45/109 (41%), Positives = 68/109 (62%)
 Frame = +2

Query: 47  YQNDEINAQAEVWKHMYGFAKTIMLRSTISLGIPDIIHNNGPVTLSQLATHLPLKSISID 226
           + N+ ++AQA +W H++ F  ++ L+S I LGIPDII+ +GP+TLS+L + LP+      
Sbjct: 8   HSNELLHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHGPMTLSELTSALPIHPTKSH 67

Query: 227 RFHHLMRYLVHMKLFTISTDHITKEDKFELTPASKLLVQGHQKSLAPYV 373
             + LMR LVH   F       T E+ + LT AS+LL++ H  SL PY+
Sbjct: 68  SVYRLMRILVHSGFFAKKKLSKTDEEGYTLTDASQLLLKDHPLSLTPYL 116


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
           glaucum]
          Length = 350

 Score = 91.7 bits (226), Expect = 6e-17
 Identities = 44/103 (42%), Positives = 74/103 (71%), Gaps = 1/103 (0%)
 Frame = +2

Query: 56  DEINAQAEVWKHMYGFAKTIMLRSTISLGIPDIIHNNG-PVTLSQLATHLPLKSISIDRF 232
           + +++QA++W  +YGFA +++L+S + L + +IIHN+G P+TLS+L+ HLP + ++ D  
Sbjct: 7   ENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQDAL 66

Query: 233 HHLMRYLVHMKLFTISTDHITKEDKFELTPASKLLVQGHQKSL 361
           + ++RYLVHMKLFT S+  I  E ++ L P +K LV+G  K +
Sbjct: 67  YRVLRYLVHMKLFTKSS--IDGELRYGLAPPAKFLVKGWDKCM 107