BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00005400
         (1287 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277768.1| PREDICTED: alpha-ketoglutarate-dependent dio...   416   e-114
emb|CAN72697.1| hypothetical protein VITISV_011566 [Vitis vinifera]   412   e-113
ref|XP_002304683.1| predicted protein [Populus trichocarpa] gi|2...   410   e-112
gb|AEL99108.1| alpha-ketoglutarate-dependent dioxygenase alkB, p...   399   e-108
gb|AEL99107.1| alpha-ketoglutarate-dependent dioxygenase alkB, p...   397   e-108

>ref|XP_002277768.1| PREDICTED: alpha-ketoglutarate-dependent dioxygenase alkB [Vitis
            vinifera] gi|297738457|emb|CBI27658.3| unnamed protein
            product [Vitis vinifera]
          Length = 360

 Score =  416 bits (1069), Expect = e-114
 Identities = 221/365 (60%), Positives = 255/365 (69%), Gaps = 32/365 (8%)
 Frame = +3

Query: 30   MYGSTGATTPDDSERTAFRXXXXXXXXXXXXXXXXXXRQKQKQPKAVDLSGVLDFKKAAQ 209
            MYGS G +  DD+ERTAFR                   +K+KQ K VDLS V+DFK   +
Sbjct: 1    MYGSYGVS--DDAERTAFRRAEKKYKVYYDDSKSS---KKKKQLKQVDLSEVVDFKAILR 55

Query: 210  -----GEIPSGIYQLHSDFDFPVFCIENQPGFYFIPGALSIEEQCHWIKESLISFPQPPN 374
                 GE+P G + L  DFD PVFCIEN+PGFYFIP AL++EEQ  WI+ESLISFPQP N
Sbjct: 56   SFNQSGEVPPGTFALQCDFDRPVFCIENRPGFYFIPDALTVEEQGRWIRESLISFPQPYN 115

Query: 375  RTNHYAIHGHIHDLFVAAQNKKVLIGE-------------------ASRWQ--------S 473
            RTNH AI+G I DLF+AA+ +K+L+ E                   A RW+        S
Sbjct: 116  RTNHNAIYGPIRDLFMAAKERKILVEEEDSSGGLDSLSNSSITNMDAERWKFYEENTVSS 175

Query: 474  SNMDQKEVAATTLLRKLRWSTLGLQFDWSKRDYNVTLPHNKIPESLCQLAKRMAAPAMGT 653
                 K ++A+ LLRKLRWSTLGLQFDWSKR+YNV+LPHNKIP++LC LAK MAAPAM  
Sbjct: 176  RGSTCKSISASVLLRKLRWSTLGLQFDWSKRNYNVSLPHNKIPDALCDLAKEMAAPAMSK 235

Query: 654  GEEFQPEAAIVNYFSSGDMLGGHLDDMEADWNKPIVSISLGCKAIFLLGGKSREDMPIAM 833
            G+ FQPEAAIVNYF  GDMLGGHLDDMEADW KPIVS+SLGCKAIFLLGGKSR+D P+AM
Sbjct: 236  GKVFQPEAAIVNYFGLGDMLGGHLDDMEADWTKPIVSMSLGCKAIFLLGGKSRDDPPLAM 295

Query: 834  FLRSGDVVLMAGQARECFHGVPRIFTDKENAEITPLALNLSHEDDVYVLDYXXXXXXXXX 1013
            F+RSGDVVLMAG+ARECFHGVPRIFTD+ENAEI PL L  SHEDDV  L+Y         
Sbjct: 296  FVRSGDVVLMAGEARECFHGVPRIFTDQENAEIAPLELMFSHEDDVCFLEYIRSSRININ 355

Query: 1014 XXQVF 1028
              QVF
Sbjct: 356  IRQVF 360


>emb|CAN72697.1| hypothetical protein VITISV_011566 [Vitis vinifera]
          Length = 366

 Score =  412 bits (1060), Expect = e-113
 Identities = 220/368 (59%), Positives = 254/368 (69%), Gaps = 35/368 (9%)
 Frame = +3

Query: 30   MYGSTGATTPDDSERTAFRXXXXXXXXXXXXXXXXXX---RQKQKQPKAVDLSGVLDFKK 200
            MYGS G +  DD+ERTAFR                       ++KQ K VDLS V+DFK 
Sbjct: 1    MYGSYGVS--DDAERTAFRRAEKKYKVYYDDSKSSKNVLENFRKKQLKQVDLSEVVDFKA 58

Query: 201  AAQ-----GEIPSGIYQLHSDFDFPVFCIENQPGFYFIPGALSIEEQCHWIKESLISFPQ 365
              +     GE+P G + L  DFD PVFCIEN+PGFYFIP AL++EEQ  WI+ESLISFPQ
Sbjct: 59   ILRSFNQSGEVPPGTFALQCDFDRPVFCIENRPGFYFIPDALTVEEQGRWIRESLISFPQ 118

Query: 366  PPNRTNHYAIHGHIHDLFVAAQNKKVLIGE-------------------ASRWQ------ 470
            P NRTNH AI+G I DLF+AA+ +K+L+ E                   A RW+      
Sbjct: 119  PYNRTNHNAIYGPIRDLFMAAKERKILVEEEDSSGGLDSLSNSSITNMDAERWKFYEENT 178

Query: 471  --SSNMDQKEVAATTLLRKLRWSTLGLQFDWSKRDYNVTLPHNKIPESLCQLAKRMAAPA 644
              S     K ++A+ LLRKLRWSTLGLQFDWSKR+YNV+LPHNKIP++LC LAK MAAPA
Sbjct: 179  VSSRGSTCKSISASVLLRKLRWSTLGLQFDWSKRNYNVSLPHNKIPDALCDLAKEMAAPA 238

Query: 645  MGTGEEFQPEAAIVNYFSSGDMLGGHLDDMEADWNKPIVSISLGCKAIFLLGGKSREDMP 824
            M  G+ FQPEAAIVNYF  GDMLGGHLDDMEADW KPIVS+SLGCKAIFLLGGKSR+D P
Sbjct: 239  MSKGKVFQPEAAIVNYFGLGDMLGGHLDDMEADWTKPIVSMSLGCKAIFLLGGKSRDDPP 298

Query: 825  IAMFLRSGDVVLMAGQARECFHGVPRIFTDKENAEITPLALNLSHEDDVYVLDYXXXXXX 1004
            +AMF+RSGDVVLMAG+ARECFHGVPRIFTD+ENAEI PL L  SHEDDV  L+Y      
Sbjct: 299  LAMFVRSGDVVLMAGEARECFHGVPRIFTDQENAEIAPLELMFSHEDDVCFLEYIRSSRI 358

Query: 1005 XXXXXQVF 1028
                 QVF
Sbjct: 359  NINIRQVF 366


>ref|XP_002304683.1| predicted protein [Populus trichocarpa] gi|222842115|gb|EEE79662.1|
            predicted protein [Populus trichocarpa]
          Length = 353

 Score =  410 bits (1053), Expect = e-112
 Identities = 219/356 (61%), Positives = 254/356 (71%), Gaps = 23/356 (6%)
 Frame = +3

Query: 30   MYGSTGATTPDDSERTAFRXXXXXXXXXXXXXXXXXXR----QKQKQPKAVDLSGVLDFK 197
            MYGS  A T DD++RT FR                        K+KQPK VDLS VLDFK
Sbjct: 1    MYGSDKAVT-DDTDRTEFRKTEKKYKLYYDQNSKRYDLIFPFLKKKQPKQVDLSEVLDFK 59

Query: 198  KAAQ-----GEIPSGIYQLHSDFDFPVFCIENQPGFYFIPGALSIEEQCHWIKESLISFP 362
                     GE+P  I  +   FD PVF +E++PGFYFIPGALS++EQC WI+ESL+SFP
Sbjct: 60   SFLDSYHQNGELPPEIVVVDCRFDRPVFGLESRPGFYFIPGALSVDEQCRWIRESLMSFP 119

Query: 363  QPPNRTNHYAIHGHIHDLFVAAQNKKVLIGEAS------RWQSSNMDQ--------KEVA 500
            QPPNRTNH AI+G I DLF+AA+ +KVL+ + +      RW     D         K V+
Sbjct: 120  QPPNRTNHNAIYGPISDLFIAAKERKVLVEDENMPANTRRWSFCEEDSVLLRGKSCKPVS 179

Query: 501  ATTLLRKLRWSTLGLQFDWSKRDYNVTLPHNKIPESLCQLAKRMAAPAMGTGEEFQPEAA 680
            A+ LLRKLRWSTLGLQFDWSKR+YNV+LPHNKIP+ LCQLAK++AAPAM  GEEF PEAA
Sbjct: 180  ASVLLRKLRWSTLGLQFDWSKRNYNVSLPHNKIPDGLCQLAKKLAAPAMPVGEEFHPEAA 239

Query: 681  IVNYFSSGDMLGGHLDDMEADWNKPIVSISLGCKAIFLLGGKSREDMPIAMFLRSGDVVL 860
            IVNYF+SGD LGGHLDDMEADW+KPIVS+SLGCKAIFLLGGKSRED P+AMFLRSGDVVL
Sbjct: 240  IVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSREDPPLAMFLRSGDVVL 299

Query: 861  MAGQARECFHGVPRIFTDKENAEITPLALNLSHEDDVYVLDYXXXXXXXXXXXQVF 1028
            MAG+ARECFHGVPRIFTDKENAEIT L L+   E+D  +L+Y           QVF
Sbjct: 300  MAGEARECFHGVPRIFTDKENAEITALELHFCDEND--ILEYIRTSRININIRQVF 353


>gb|AEL99108.1| alpha-ketoglutarate-dependent dioxygenase alkB, partial [Silene
            latifolia]
          Length = 354

 Score =  399 bits (1024), Expect = e-108
 Identities = 209/358 (58%), Positives = 250/358 (69%), Gaps = 28/358 (7%)
 Frame = +3

Query: 39   STGATTPDDSERTAFRXXXXXXXXXXXXXXXXXXRQKQKQPKAVDLSGVLDFKKAAQ--- 209
            + GAT  DD ERTAFR                  R+K++QP+ VDLS V+DFK   +   
Sbjct: 3    ANGAT--DDLERTAFRRAEKKYKLYYGQNS----RKKKQQPRPVDLSEVVDFKLILETFD 56

Query: 210  --GEIPSGIYQLHSDFDFPVFCIENQPGFYFIPGALSIEEQCHWIKESLISFPQPPNRTN 383
              GE P G++ + +  +FPV C++N+PGFYFIPGALS+EEQC WI+ESL SFPQPPNRTN
Sbjct: 57   RTGETPDGVHVIRTGSEFPVICLDNRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTN 116

Query: 384  HYAIHGHIHDLFVAAQNKKVLI--------------GEASRW----QSSNMDQK-----E 494
            H A++G I+DLF AAQ  KVL+              G+A RW    +S N+  K      
Sbjct: 117  HNALYGPINDLFAAAQEGKVLVEEELSPNVTQSLDNGQARRWTFSSESGNVSLKGSGGNS 176

Query: 495  VAATTLLRKLRWSTLGLQFDWSKRDYNVTLPHNKIPESLCQLAKRMAAPAMGTGEEFQPE 674
            + A+ LLRKL WSTLGLQFDWS+R+YNV LPHNKIP+ LCQLAK++A PAM  G+ F+PE
Sbjct: 177  ILASVLLRKLHWSTLGLQFDWSQRNYNVDLPHNKIPDKLCQLAKKLAVPAMPLGDNFKPE 236

Query: 675  AAIVNYFSSGDMLGGHLDDMEADWNKPIVSISLGCKAIFLLGGKSREDMPIAMFLRSGDV 854
            AAIVNYF+SGD LGGHLDDME DW+KPIVS+SLGCK IFLLGGKSRED P+AMFLRSGDV
Sbjct: 237  AAIVNYFASGDTLGGHLDDMELDWSKPIVSMSLGCKGIFLLGGKSREDEPLAMFLRSGDV 296

Query: 855  VLMAGQARECFHGVPRIFTDKENAEITPLALNLSHEDDVYVLDYXXXXXXXXXXXQVF 1028
            VLMAG+ARECFHGVPRIFTD  N+EIT L  +LS + D   LDY           QVF
Sbjct: 297  VLMAGEARECFHGVPRIFTDAHNSEITALEKHLSTDSDHCFLDYIKTSRININIRQVF 354


>gb|AEL99107.1| alpha-ketoglutarate-dependent dioxygenase alkB, partial [Silene
            latifolia]
          Length = 354

 Score =  397 bits (1019), Expect = e-108
 Identities = 209/358 (58%), Positives = 250/358 (69%), Gaps = 28/358 (7%)
 Frame = +3

Query: 39   STGATTPDDSERTAFRXXXXXXXXXXXXXXXXXXRQKQKQPKAVDLSGVLDFKKAAQ--- 209
            + GAT  DD ERTAFR                  R+K++QP+ VDLS V+DFK   +   
Sbjct: 3    ANGAT--DDLERTAFRRAEKKYKLYYGQNS----RKKKQQPRPVDLSEVVDFKLILETFD 56

Query: 210  --GEIPSGIYQLHSDFDFPVFCIENQPGFYFIPGALSIEEQCHWIKESLISFPQPPNRTN 383
              GE P G++ + +  +FPV C++N+PGFYFIPGALS+EEQC WI+ESL SFPQPPNRTN
Sbjct: 57   RTGETPDGVHVIRTGSEFPVICLDNRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTN 116

Query: 384  HYAIHGHIHDLFVAAQNKKVLI--------------GEASRW----QSSNMDQK-----E 494
            H A++G I+DLF AAQ  KVL+              G+A RW    +S N+  K      
Sbjct: 117  HNALYGPINDLFAAAQEGKVLVEEELSPNVTQSLDNGQARRWTFSSESGNVSLKGSGGNS 176

Query: 495  VAATTLLRKLRWSTLGLQFDWSKRDYNVTLPHNKIPESLCQLAKRMAAPAMGTGEEFQPE 674
            + A+ LLRKLRWSTLGLQFDWS+R+YNV L HNKIP+ LCQLAK++A PAM  G+ F+PE
Sbjct: 177  ILASVLLRKLRWSTLGLQFDWSQRNYNVDLLHNKIPDKLCQLAKKLAVPAMPLGDNFKPE 236

Query: 675  AAIVNYFSSGDMLGGHLDDMEADWNKPIVSISLGCKAIFLLGGKSREDMPIAMFLRSGDV 854
            AAIVNYF+SGD LGGHLDDME DW+KPIVS+SLGCK IFLLGGKSRED P+AMFLRSGDV
Sbjct: 237  AAIVNYFASGDTLGGHLDDMELDWSKPIVSMSLGCKGIFLLGGKSREDEPLAMFLRSGDV 296

Query: 855  VLMAGQARECFHGVPRIFTDKENAEITPLALNLSHEDDVYVLDYXXXXXXXXXXXQVF 1028
            VLMAG+ARECFHGVPRIFTD  N+EIT L  +LS + D   LDY           QVF
Sbjct: 297  VLMAGEARECFHGVPRIFTDAHNSEITALEKHLSTDSDHCFLDYIKTSRININIRQVF 354