BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00005274
         (1171 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   556   0.0  
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   511   e-172
dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       426   e-146
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       411   e-137
sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Sh...   381   e-128

>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
           Full=(S)-tetrahydrocolumbamine oxidase
           (methylenedioxy-bridge-forming); AltName:
           Full=Cytochrome P450 719A1 gi|15822820|dbj|BAB68769.1|
           methylenedioxy bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  556 bits (1434), Expect(2) = 0.0
 Identities = 281/296 (94%), Positives = 283/296 (95%)
 Frame = +3

Query: 3   IGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISDLEKAWEVLVNKSADYGAR 182
           IGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISD+EKAWEVLVNKSADYGAR
Sbjct: 46  IGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVLVNKSADYGAR 105

Query: 183 DMPEITKIASASWHTISTSDAGPFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAM 362
           DMPEITKIASASWHTISTSDAG FWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAM
Sbjct: 106 DMPEITKIASASWHTISTSDAGSFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAM 165

Query: 363 SDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQSFDDNKFIESMHYEIEDIIRISGYARLA 542
           SDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQ+FDDNKFIESMHYEIEDIIRISGYARLA
Sbjct: 166 SDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQAFDDNKFIESMHYEIEDIIRISGYARLA 225

Query: 543 EAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQNXXXXXXXXX 722
           EAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQN         
Sbjct: 226 EAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQNFEEEVIIFC 285

Query: 723 XXXLYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLK 890
              LYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLK
Sbjct: 286 IFELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLK 341



 Score =  131 bits (329), Expect(2) = 0.0
 Identities = 61/62 (98%), Positives = 62/62 (100%)
 Frame = +2

Query: 986  TMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPHKFMPERFLE 1165
            TMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDP+KFMPERFLE
Sbjct: 350  TMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFLE 409

Query: 1166 GE 1171
            GE
Sbjct: 410  GE 411


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  511 bits (1315), Expect(2) = e-172
 Identities = 252/296 (85%), Positives = 272/296 (91%)
 Frame = +3

Query: 3   IGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISDLEKAWEVLVNKSADYGAR 182
           IGNLHQLG DVLHVALAKLA+VHGSVMT+WIG+WRP+IVISD++KAWEVLVNKS+DYGAR
Sbjct: 47  IGNLHQLGGDVLHVALAKLAQVHGSVMTVWIGNWRPIIVISDIDKAWEVLVNKSSDYGAR 106

Query: 183 DMPEITKIASASWHTISTSDAGPFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAM 362
           DMPEITKIASASWHTISTSDAG FWQ VRKGLQSGAMGPLN+AAQ+Q+QERDMKRLI+A+
Sbjct: 107 DMPEITKIASASWHTISTSDAGAFWQTVRKGLQSGAMGPLNIAAQSQFQERDMKRLIQAL 166

Query: 363 SDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQSFDDNKFIESMHYEIEDIIRISGYARLA 542
            DEAA NN IVKPLDHIKKNTVRLLTRLIFGQ+FDD+KF+ESMH+EI+DIIRISGYARLA
Sbjct: 167 RDEAAQNNNIVKPLDHIKKNTVRLLTRLIFGQTFDDDKFVESMHFEIDDIIRISGYARLA 226

Query: 543 EAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQNXXXXXXXXX 722
           EAFYYAKYLPSHKKAEREA+LVKCRVE+LV PLLSS PPTNSYLYFLLSQ          
Sbjct: 227 EAFYYAKYLPSHKKAEREAYLVKCRVEDLVSPLLSSNPPTNSYLYFLLSQRFSEEVIIFC 286

Query: 723 XXXLYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLK 890
              LYLLGVDSTSSTTTWALAYLIREQ  QEKLYQD RMTLGDVDLVKIEDV+KLK
Sbjct: 287 IFELYLLGVDSTSSTTTWALAYLIREQAIQEKLYQDTRMTLGDVDLVKIEDVSKLK 342



 Score =  122 bits (306), Expect(2) = e-172
 Identities = 56/62 (90%), Positives = 61/62 (98%)
 Frame = +2

Query: 986  TMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPHKFMPERFLE 1165
            TMRMKPIAPLAIPHKTAK+TTLMG+KVAKGTRIMVNLYALHHNQNIW +P+KFMPERFL+
Sbjct: 351  TMRMKPIAPLAIPHKTAKDTTLMGSKVAKGTRIMVNLYALHHNQNIWTNPYKFMPERFLQ 410

Query: 1166 GE 1171
            GE
Sbjct: 411  GE 412


>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  426 bits (1095), Expect(2) = e-146
 Identities = 210/296 (70%), Positives = 241/296 (81%)
 Frame = +3

Query: 3   IGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISDLEKAWEVLVNKSADYGAR 182
           IGNLH LG D+LHV LAKLAKVHGSVMTIWIG WRP+IVISD+  AWEVLV+KSA+YG R
Sbjct: 46  IGNLHLLGGDLLHVTLAKLAKVHGSVMTIWIGRWRPIIVISDINSAWEVLVSKSAEYGQR 105

Query: 183 DMPEITKIASASWHTISTSDAGPFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAM 362
           D P+I K  +   + IS SD GPFW NVRKGLQ+GA+ PLNVAAQ Q++E D+KRLI  M
Sbjct: 106 DTPDIFKTLTVGQNNISMSDIGPFWHNVRKGLQNGALSPLNVAAQTQFREEDVKRLIHEM 165

Query: 363 SDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQSFDDNKFIESMHYEIEDIIRISGYARLA 542
           S EA N NGI+KPLDH+KKNT RLL RLIFG +FD++KF+ES+ YEIED++R+SGYARL 
Sbjct: 166 SREALNKNGIIKPLDHMKKNTARLLGRLIFGPTFDNDKFVESVLYEIEDVLRLSGYARLT 225

Query: 543 EAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQNXXXXXXXXX 722
           EAFYYAKYLPS KK  REA+L K R+EEL+RPLLSS PPTNSYLYFL SQN         
Sbjct: 226 EAFYYAKYLPSQKKVAREAYLAKYRIEELMRPLLSSNPPTNSYLYFLQSQNYSKEIIIFC 285

Query: 723 XXXLYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLK 890
              L+LLGVDSTSST TWALAYLI EQ  QEKL +DIRMT+GDVDLVKIED++KLK
Sbjct: 286 TFELFLLGVDSTSSTATWALAYLIHEQAVQEKLNEDIRMTIGDVDLVKIEDLSKLK 341



 Score =  119 bits (299), Expect(2) = e-146
 Identities = 53/62 (85%), Positives = 61/62 (98%)
 Frame = +2

Query: 986  TMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPHKFMPERFLE 1165
            TMRMKPIAPLAIPHKTA +TTLMGTKVA+GT+IMVNLYALHHN++IWP+P+KFMPERFL+
Sbjct: 350  TMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALHHNEDIWPEPYKFMPERFLQ 409

Query: 1166 GE 1171
            GE
Sbjct: 410  GE 411


>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  411 bits (1056), Expect(2) = e-137
 Identities = 208/299 (69%), Positives = 239/299 (79%), Gaps = 3/299 (1%)
 Frame = +3

Query: 3   IGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISDLEKAWEVLVNKSADYGAR 182
           IGNLHQL  ++LHV LAKLAKVHG VMTIW+GSW P+IVISD+  AWEVLVNKSADYGAR
Sbjct: 59  IGNLHQLRGELLHVTLAKLAKVHGGVMTIWMGSWVPIIVISDINSAWEVLVNKSADYGAR 118

Query: 183 DMP---EITKIASASWHTISTSDAGPFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLI 353
           ++    E+ + ++   H IS+SD GP W N+RKGLQSGA+ PL+VA+Q Q++ERDMKR++
Sbjct: 119 EVTYLHELLRDSAMHPHNISSSDVGPLWHNIRKGLQSGALSPLSVASQTQHRERDMKRVV 178

Query: 354 KAMSDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQSFDDNKFIESMHYEIEDIIRISGYA 533
             MS E  NNNG +KPL HIK +T RLL RLIFG SF+D+KFIESM YEIED IR S Y 
Sbjct: 179 HEMSREIVNNNGTIKPLVHIKNSTARLLGRLIFGPSFNDSKFIESMLYEIEDFIRTSSYT 238

Query: 534 RLAEAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQNXXXXXX 713
            L  +FYYAKYLPS +KA REA L K R+EELVRPLLSSKPPTN+YLYFLLSQN      
Sbjct: 239 SLTNSFYYAKYLPSQRKATREAKLGKYRIEELVRPLLSSKPPTNAYLYFLLSQNLSEEVT 298

Query: 714 XXXXXXLYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLK 890
                 L+LLGVDS SSTTTWALAY+IREQ AQEKLYQDIRMT+GDVDLVKIEDVNKLK
Sbjct: 299 IFCIFELFLLGVDSASSTTTWALAYMIREQEAQEKLYQDIRMTIGDVDLVKIEDVNKLK 357



 Score =  106 bits (264), Expect(2) = e-137
 Identities = 45/62 (72%), Positives = 55/62 (88%)
 Frame = +2

Query: 986  TMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPHKFMPERFLE 1165
            TMRMKPIAPLA+PH   K+TTLMGTK+ KGTR+MVNLYALHH +++W +P KFMPER+L+
Sbjct: 366  TMRMKPIAPLALPHNAGKDTTLMGTKIVKGTRVMVNLYALHHYEDVWLEPSKFMPERYLQ 425

Query: 1166 GE 1171
            GE
Sbjct: 426  GE 427


>sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Short=STS; AltName:
           Full=Cytochrome P450 719A13 gi|148841092|gb|ABR14721.1|
           stylopine synthase [Argemone mexicana]
          Length = 504

 Score =  381 bits (979), Expect(2) = e-128
 Identities = 185/298 (62%), Positives = 235/298 (78%), Gaps = 2/298 (0%)
 Frame = +3

Query: 3   IGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISDLEKAWEVLVNKSADYGAR 182
           IGN++ LG   LHV L  LAK +G+VMTIWIGSWRPVIV+SD+++AWEVLVNKS+DY AR
Sbjct: 58  IGNMNILGGTALHVVLHNLAKTYGNVMTIWIGSWRPVIVVSDIDRAWEVLVNKSSDYSAR 117

Query: 183 DMPEITKIASASWHTISTSDAGPFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAM 362
           DMPEITK+A+A W TIS+SD+GPFW N+RKGLQ+ A+ P N+++Q+++QERD+ + I+ +
Sbjct: 118 DMPEITKLATADWKTISSSDSGPFWTNLRKGLQNVALSPQNLSSQSKFQERDIIKTIQNL 177

Query: 363 SDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQSFDDNKFIESMHYEIEDIIRISGYARLA 542
            +EA  NNGIVKPLDH+KK  VRL++RLI+GQ FD+++++E MH+ IE++IR+SGYARLA
Sbjct: 178 KEEAKMNNGIVKPLDHLKKAMVRLISRLIYGQDFDNDEYVEEMHHTIEELIRVSGYARLA 237

Query: 543 EAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQNXXXXXXXXX 722
           EAFYYAKYLPSHKKA RE      RV+ LVRPLLS   PTN+YL+FL SQN         
Sbjct: 238 EAFYYAKYLPSHKKAVREVLQANQRVQNLVRPLLSLNSPTNTYLHFLRSQNYEDEVIIFA 297

Query: 723 XXXLYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVD--LVKIEDVNKLK 890
               YLLGVDSTSSTT WALAYLIRE   QEKLY++++    D D  +VK ED+NKL+
Sbjct: 298 IFEAYLLGVDSTSSTTAWALAYLIREPNVQEKLYEELKNFTNDNDRKMVKFEDLNKLQ 355



 Score =  104 bits (260), Expect(2) = e-128
 Identities = 45/60 (75%), Positives = 54/60 (90%)
 Frame = +2

Query: 986  TMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPHKFMPERFLE 1165
            TMRMKPIAPLAIPHK  +ET+LMG KV +GTR+MVN+YALHHNQN+W +P+KF PERFL+
Sbjct: 364  TMRMKPIAPLAIPHKACRETSLMGRKVNQGTRVMVNIYALHHNQNVWKEPYKFNPERFLQ 423