BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00005175
         (1656 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002274128.1| PREDICTED: sterol 14-demethylase [Vitis vini...   484   e-144
gb|ABC59074.1| cytochrome P450 monooxygenase CYP51G1 [Medicago t...   479   e-143
ref|XP_002299356.1| cytochrome P450 obtusifoliol 14-alpha-demeth...   477   e-143
gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]   478   e-142
ref|XP_002512680.1| cytochrome P450, putative [Ricinus communis]...   477   e-142

>ref|XP_002274128.1| PREDICTED: sterol 14-demethylase [Vitis vinifera]
          Length = 486

 Score =  484 bits (1245), Expect(2) = e-144
 Identities = 239/282 (84%), Positives = 253/282 (89%)
 Frame = +1

Query: 589  YFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDLDSGMLPISVIF 768
            YFSKWGD GEVDLKYELEHLIILTASRCLLG+E+R+KLF DVSALFHDLD+GMLPISVIF
Sbjct: 156  YFSKWGDCGEVDLKYELEHLIILTASRCLLGQEIRNKLFADVSALFHDLDNGMLPISVIF 215

Query: 769  PYLPIPAHRRRDQARKKLSEIFAKIISSRKETGKSENDMLQCFIESKYKDGRPTTESEVT 948
            PYLPIPAHRRRDQARKKL+EIFA II+SRKETGKSENDMLQCFI SKYKDGRPTTESEVT
Sbjct: 216  PYLPIPAHRRRDQARKKLAEIFANIIASRKETGKSENDMLQCFIASKYKDGRPTTESEVT 275

Query: 949  GLLIAALFAGQHTSSITSTWTGAYLLQNKNCLSEVLDEQKTLMKKYGDNVDHDILSEMDV 1128
            GLLIAALFAGQHTSSITSTWTGAYLL++K  LS V DEQ++LMKKYG  VDHDILSEMDV
Sbjct: 276  GLLIAALFAGQHTSSITSTWTGAYLLRHKEYLSAVQDEQRSLMKKYGSKVDHDILSEMDV 335

Query: 1129 LYRSIKEAXXXXXXXXXXXXXXXSDFSVTTREGKEYDIPKGHIVAASPAFANRLPHIFKD 1308
            LYR IKEA               +DFSVTTR+GKEYDIPKGHIVA SPAFANRLPHI+KD
Sbjct: 336  LYRCIKEALRLHPPLIMLLRSSHTDFSVTTRDGKEYDIPKGHIVATSPAFANRLPHIYKD 395

Query: 1309 PDVYDPNRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYL 1434
            PD YDP+RFA GREEDKAAGAFSYISFGGGRHGCLGEPFAYL
Sbjct: 396  PDRYDPDRFAVGREEDKAAGAFSYISFGGGRHGCLGEPFAYL 437



 Score = 57.4 bits (137), Expect(2) = e-144
 Identities = 26/42 (61%), Positives = 30/42 (71%)
 Frame = +3

Query: 1527 HLLRNFELELISPFPEIDWNAXXXXXXXXXXXRYKRRQLSLD 1652
            HLLRNFELELISPFPE+DWNA           RYKRR+L ++
Sbjct: 445  HLLRNFELELISPFPEVDWNAMVVGVKGKVMVRYKRRELPVN 486


>gb|ABC59074.1| cytochrome P450 monooxygenase CYP51G1 [Medicago truncatula]
          Length = 489

 Score =  479 bits (1232), Expect(2) = e-143
 Identities = 238/282 (84%), Positives = 249/282 (88%)
 Frame = +1

Query: 589  YFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDLDSGMLPISVIF 768
            YFSKWG SGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDLD+GMLPISV+F
Sbjct: 158  YFSKWGSSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDLDNGMLPISVLF 217

Query: 769  PYLPIPAHRRRDQARKKLSEIFAKIISSRKETGKSENDMLQCFIESKYKDGRPTTESEVT 948
            PYLPIPAHRRRD ARKKL+EIFA IISSRK   KSE+DMLQCFI+SKYKDGRPTTESEVT
Sbjct: 218  PYLPIPAHRRRDNARKKLAEIFANIISSRKIADKSEDDMLQCFIDSKYKDGRPTTESEVT 277

Query: 949  GLLIAALFAGQHTSSITSTWTGAYLLQNKNCLSEVLDEQKTLMKKYGDNVDHDILSEMDV 1128
            GLLIAALFAGQHTSSITSTWTGAYLL NK  LS V +EQK LM+K+GD VDHD+L+EMDV
Sbjct: 278  GLLIAALFAGQHTSSITSTWTGAYLLCNKQYLSAVEEEQKNLMEKHGDRVDHDVLAEMDV 337

Query: 1129 LYRSIKEAXXXXXXXXXXXXXXXSDFSVTTREGKEYDIPKGHIVAASPAFANRLPHIFKD 1308
            LYR IKEA               SDFSVTTREGKEYDIPKGHIVA SPAFANRLPHIF D
Sbjct: 338  LYRCIKEALRLHPPLIMLLRSSHSDFSVTTREGKEYDIPKGHIVATSPAFANRLPHIFND 397

Query: 1309 PDVYDPNRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYL 1434
            PD YDP+RFA GREEDKAAGAFSYISFGGGRHGCLGEPFAYL
Sbjct: 398  PDKYDPDRFAVGREEDKAAGAFSYISFGGGRHGCLGEPFAYL 439



 Score = 59.3 bits (142), Expect(2) = e-143
 Identities = 27/42 (64%), Positives = 31/42 (73%)
 Frame = +3

Query: 1527 HLLRNFELELISPFPEIDWNAXXXXXXXXXXXRYKRRQLSLD 1652
            HLLRNFELEL+SPFPEIDWNA           RYKRR+LS++
Sbjct: 447  HLLRNFELELVSPFPEIDWNAMVVGVKGKVMVRYKRRELSVN 488


>ref|XP_002299356.1| cytochrome P450 obtusifoliol 14-alpha-demethylase [Populus
            trichocarpa] gi|222846614|gb|EEE84161.1| cytochrome P450
            obtusifoliol 14-alpha-demethylase [Populus trichocarpa]
          Length = 488

 Score =  477 bits (1227), Expect(2) = e-143
 Identities = 234/282 (82%), Positives = 251/282 (89%)
 Frame = +1

Query: 589  YFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDLDSGMLPISVIF 768
            YF KWGDSG VDLKYELEHLIILTASRCLLGREVRDKLFDDV+ALFHDLD+GMLP+SV+F
Sbjct: 158  YFLKWGDSGVVDLKYELEHLIILTASRCLLGREVRDKLFDDVAALFHDLDNGMLPVSVLF 217

Query: 769  PYLPIPAHRRRDQARKKLSEIFAKIISSRKETGKSENDMLQCFIESKYKDGRPTTESEVT 948
            PYLPIPAHRRRD+ARKKL+EIFA II+SRK   KSENDMLQCFI+SKYKDGRPTTESE+T
Sbjct: 218  PYLPIPAHRRRDRARKKLAEIFANIINSRKLASKSENDMLQCFIDSKYKDGRPTTESEIT 277

Query: 949  GLLIAALFAGQHTSSITSTWTGAYLLQNKNCLSEVLDEQKTLMKKYGDNVDHDILSEMDV 1128
            GLLIAALFAGQHTSSITSTWTGAYLL++   LS VL+EQK LMKK+G+ VDHDILSEMDV
Sbjct: 278  GLLIAALFAGQHTSSITSTWTGAYLLRHNEYLSAVLEEQKNLMKKHGNKVDHDILSEMDV 337

Query: 1129 LYRSIKEAXXXXXXXXXXXXXXXSDFSVTTREGKEYDIPKGHIVAASPAFANRLPHIFKD 1308
            LYR IKEA               SDFSVTTR+GKEYDIPKGHIVA SPAFANRLPH+FKD
Sbjct: 338  LYRCIKEALRLHPPLIMLLRSSHSDFSVTTRDGKEYDIPKGHIVATSPAFANRLPHVFKD 397

Query: 1309 PDVYDPNRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYL 1434
            PD YDP+RFA GREEDKAAGAFSYISFGGGRHGCLGEPFAYL
Sbjct: 398  PDSYDPDRFAYGREEDKAAGAFSYISFGGGRHGCLGEPFAYL 439



 Score = 59.7 bits (143), Expect(2) = e-143
 Identities = 28/42 (66%), Positives = 31/42 (73%)
 Frame = +3

Query: 1527 HLLRNFELELISPFPEIDWNAXXXXXXXXXXXRYKRRQLSLD 1652
            HLLRNFELELISPFPEIDWNA           RYKRR+LS++
Sbjct: 447  HLLRNFELELISPFPEIDWNAMVVGVKDKVMVRYKRRELSVN 488


>gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]
          Length = 487

 Score =  478 bits (1229), Expect(2) = e-142
 Identities = 234/282 (82%), Positives = 250/282 (88%)
 Frame = +1

Query: 589  YFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDLDSGMLPISVIF 768
            YFSKWG+SGEVDLKYELEHLIILTASRCLLG EVR+KLFDDVSALFHDLD+GMLPISVIF
Sbjct: 157  YFSKWGESGEVDLKYELEHLIILTASRCLLGEEVRNKLFDDVSALFHDLDNGMLPISVIF 216

Query: 769  PYLPIPAHRRRDQARKKLSEIFAKIISSRKETGKSENDMLQCFIESKYKDGRPTTESEVT 948
            PYLPIPAHRRRD ARKKL+EIFA II+SRK TGK+ENDMLQCFI+SKYKDGRPTTE E+T
Sbjct: 217  PYLPIPAHRRRDNARKKLAEIFANIINSRKRTGKAENDMLQCFIDSKYKDGRPTTEGEIT 276

Query: 949  GLLIAALFAGQHTSSITSTWTGAYLLQNKNCLSEVLDEQKTLMKKYGDNVDHDILSEMDV 1128
            GLLIAALFAGQHTSSITSTWTG+YLL N   +S V+DEQK LMKK+G+ VDHDILSEMDV
Sbjct: 277  GLLIAALFAGQHTSSITSTWTGSYLLTNNKYMSAVVDEQKNLMKKHGNKVDHDILSEMDV 336

Query: 1129 LYRSIKEAXXXXXXXXXXXXXXXSDFSVTTREGKEYDIPKGHIVAASPAFANRLPHIFKD 1308
            LYR IKEA               SDFSVTTREGKEYDIPKGHIVA SPAFANRLPHIFK+
Sbjct: 337  LYRCIKEALRLHPPLIMLLRSSHSDFSVTTREGKEYDIPKGHIVATSPAFANRLPHIFKN 396

Query: 1309 PDVYDPNRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYL 1434
            P+ YDP+RF  GREEDKAAGAFSYISFGGGRHGCLGEPFAYL
Sbjct: 397  PESYDPDRFGPGREEDKAAGAFSYISFGGGRHGCLGEPFAYL 438



 Score = 56.2 bits (134), Expect(2) = e-142
 Identities = 26/42 (61%), Positives = 29/42 (69%)
 Frame = +3

Query: 1527 HLLRNFELELISPFPEIDWNAXXXXXXXXXXXRYKRRQLSLD 1652
            HLLRNFE ELISPFPEIDWNA           +YKRR+LS +
Sbjct: 446  HLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYKRRKLSAE 487


>ref|XP_002512680.1| cytochrome P450, putative [Ricinus communis]
            gi|223548641|gb|EEF50132.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 486

 Score =  477 bits (1228), Expect(2) = e-142
 Identities = 235/284 (82%), Positives = 255/284 (89%)
 Frame = +1

Query: 583  QVYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDLDSGMLPISV 762
            Q YFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDLD+GMLPISV
Sbjct: 154  QDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSALFHDLDNGMLPISV 213

Query: 763  IFPYLPIPAHRRRDQARKKLSEIFAKIISSRKETGKSENDMLQCFIESKYKDGRPTTESE 942
            +FPYLPIPAHRRRD+ARKKL+EIFA II+SRK  GK+ENDMLQCFI+SKYKDGRPTTE+E
Sbjct: 214  LFPYLPIPAHRRRDRARKKLAEIFANIIASRKLAGKTENDMLQCFIDSKYKDGRPTTEAE 273

Query: 943  VTGLLIAALFAGQHTSSITSTWTGAYLLQNKNCLSEVLDEQKTLMKKYGDNVDHDILSEM 1122
            VTGLLIAALFAGQHTSSITSTWTGAYLL+ K  LS VL+EQKTLM+K+G+ VDHDILSEM
Sbjct: 274  VTGLLIAALFAGQHTSSITSTWTGAYLLRYKEYLSAVLEEQKTLMEKHGNKVDHDILSEM 333

Query: 1123 DVLYRSIKEAXXXXXXXXXXXXXXXSDFSVTTREGKEYDIPKGHIVAASPAFANRLPHIF 1302
            DVLYR IKEA               SDFSV TR+GKEYDIPKGHIVA SPAFANRLPHI+
Sbjct: 334  DVLYRCIKEALRLHPPLIMLLRSSHSDFSVKTRDGKEYDIPKGHIVATSPAFANRLPHIY 393

Query: 1303 KDPDVYDPNRFAAGREEDKAAGAFSYISFGGGRHGCLGEPFAYL 1434
            K+PD YDP+R++AGREEDK AGAFSYISFGGGRHGCLGEPFA+L
Sbjct: 394  KNPDRYDPDRYSAGREEDKVAGAFSYISFGGGRHGCLGEPFAFL 437



 Score = 55.8 bits (133), Expect(2) = e-142
 Identities = 25/42 (59%), Positives = 29/42 (69%)
 Frame = +3

Query: 1527 HLLRNFELELISPFPEIDWNAXXXXXXXXXXXRYKRRQLSLD 1652
            HLLRNFE EL+SPFPE DWNA           RYKRR+LS++
Sbjct: 445  HLLRNFEFELVSPFPETDWNAMVVGVKGKVMVRYKRRELSVN 486