BLASTX nr result
ID: Coptis23_contig00004980
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00004980
(1758 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 540 e-151
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 540 e-151
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 540 e-151
emb|CBI34715.3| unnamed protein product [Vitis vinifera] 538 e-150
ref|XP_002298058.1| cytochrome P450 [Populus trichocarpa] gi|222... 522 e-145
>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Vitis vinifera]
Length = 503
Score = 540 bits (1391), Expect = e-151
Identities = 268/467 (57%), Positives = 348/467 (74%), Gaps = 2/467 (0%)
Frame = -1
Query: 1575 IKSPSNLPPGPFPWPVIGNILNMRNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAA 1396
+KSP LPPGP PWP++GN+L++ N PHISLAR +++YGPLISLRLG+Q++VV S+ +AA
Sbjct: 39 LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 97
Query: 1395 TEILKTHDRILSARCVPHVIPVYPDQIH-FSMAW-VDCSDQWRYLRTLCRTELFSAKAIA 1222
EILKTHDR+LS R VPH +P +I+ S+ W V+C+ W+ LRT+CR ELFS K +
Sbjct: 98 MEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVME 157
Query: 1221 SQASVRDKKTNELVEFLAKKEGKLVNIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGM 1042
SQA V +KK E+V F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++++DGGM
Sbjct: 158 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGM 217
Query: 1041 KGLLRRFAELGSTPNLADFYPILSGLDLQGITKKSKEMFGQIIGVFSVITKQRREDKGND 862
KGL+R+ + PNL DFY I SGLDLQG+ KK+KE+ +I ++ + ++RRE +D
Sbjct: 218 KGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 277
Query: 861 VSRQRXXXXXXXXXXXXXDQINYLIQELFLAGADTSSSTVEWAMTELIKNPKAMRKVCAE 682
S+Q DQIN L EL AGADTSSST+EWAM ELIK+P++M+KV E
Sbjct: 278 PSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEE 336
Query: 681 LERETSGHIVKESELPHLPYLHSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQV 502
L RE S ++ K S+LPHLPYL + V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+
Sbjct: 337 LAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQI 396
Query: 501 LVNAWAIGRDPSTWEDPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAATQVT 322
VNAWAIGRDP WEDPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA +
Sbjct: 397 WVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLP 456
Query: 321 LILACLINCFDWLLPHDMLLSELDMDDKIGITSQKKKPLVLIPKVTK 181
LILA L + FDW LP+ ELDM+DK G+T QK++PL++IPKV K
Sbjct: 457 LILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKVRK 503
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 540 bits (1390), Expect = e-151
Identities = 269/467 (57%), Positives = 347/467 (74%), Gaps = 2/467 (0%)
Frame = -1
Query: 1575 IKSPSNLPPGPFPWPVIGNILNMRNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAA 1396
+KSP LPPGP PWP++GN+L++ N PHISLAR +++YGPLISLRLG+Q++VV S+ +AA
Sbjct: 472 LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 530
Query: 1395 TEILKTHDRILSARCVPHVIPVYPDQIH-FSMAW-VDCSDQWRYLRTLCRTELFSAKAIA 1222
EILKTHDR+LS R VPH +P +I+ S+ W V+C+D W+ LRT+CR ELFS K +
Sbjct: 531 MEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVME 590
Query: 1221 SQASVRDKKTNELVEFLAKKEGKLVNIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGM 1042
SQA V +KK E+V F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++ +DGGM
Sbjct: 591 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGM 650
Query: 1041 KGLLRRFAELGSTPNLADFYPILSGLDLQGITKKSKEMFGQIIGVFSVITKQRREDKGND 862
KGL+R+ + PNL DFY I SGLDLQG+ KK+KE+ +I ++ + ++RRE +D
Sbjct: 651 KGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 710
Query: 861 VSRQRXXXXXXXXXXXXXDQINYLIQELFLAGADTSSSTVEWAMTELIKNPKAMRKVCAE 682
S+Q DQIN L EL AGADTSSST+EWAM ELIK+P++M+KV E
Sbjct: 711 PSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEE 769
Query: 681 LERETSGHIVKESELPHLPYLHSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQV 502
L RE S ++ K S+LPHLPYL + V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+
Sbjct: 770 LAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQI 829
Query: 501 LVNAWAIGRDPSTWEDPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAATQVT 322
VNAWAIGRDP WEDPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA +
Sbjct: 830 WVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLP 889
Query: 321 LILACLINCFDWLLPHDMLLSELDMDDKIGITSQKKKPLVLIPKVTK 181
LILA L + FDW LP+ ELDM+DK GI QK++PL++IPKV K
Sbjct: 890 LILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 936
>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 503
Score = 540 bits (1390), Expect = e-151
Identities = 269/467 (57%), Positives = 347/467 (74%), Gaps = 2/467 (0%)
Frame = -1
Query: 1575 IKSPSNLPPGPFPWPVIGNILNMRNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAA 1396
+KSP LPPGP PWP++GN+L++ N PHISLAR +++YGPLISLRLG+Q++VV S+ +AA
Sbjct: 39 LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 97
Query: 1395 TEILKTHDRILSARCVPHVIPVYPDQIH-FSMAW-VDCSDQWRYLRTLCRTELFSAKAIA 1222
EILKTHDR+LS R VPH +P +I+ S+ W V+C+D W+ LRT+CR ELFS K +
Sbjct: 98 MEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVME 157
Query: 1221 SQASVRDKKTNELVEFLAKKEGKLVNIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGM 1042
SQA V +KK E+V F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++ +DGGM
Sbjct: 158 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGM 217
Query: 1041 KGLLRRFAELGSTPNLADFYPILSGLDLQGITKKSKEMFGQIIGVFSVITKQRREDKGND 862
KGL+R+ + PNL DFY I SGLDLQG+ KK+KE+ +I ++ + ++RRE +D
Sbjct: 218 KGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 277
Query: 861 VSRQRXXXXXXXXXXXXXDQINYLIQELFLAGADTSSSTVEWAMTELIKNPKAMRKVCAE 682
S+Q DQIN L EL AGADTSSST+EWAM ELIK+P++M+KV E
Sbjct: 278 PSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEE 336
Query: 681 LERETSGHIVKESELPHLPYLHSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQV 502
L RE S ++ K S+LPHLPYL + V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+
Sbjct: 337 LAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQI 396
Query: 501 LVNAWAIGRDPSTWEDPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAATQVT 322
VNAWAIGRDP WEDPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA +
Sbjct: 397 WVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLP 456
Query: 321 LILACLINCFDWLLPHDMLLSELDMDDKIGITSQKKKPLVLIPKVTK 181
LILA L + FDW LP+ ELDM+DK GI QK++PL++IPKV K
Sbjct: 457 LILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 503
>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
Length = 1076
Score = 538 bits (1385), Expect = e-150
Identities = 267/467 (57%), Positives = 347/467 (74%), Gaps = 2/467 (0%)
Frame = -1
Query: 1575 IKSPSNLPPGPFPWPVIGNILNMRNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAA 1396
+KSP LPPGP PWP++GN+L++ N PHISLAR +++YGPLISLRLG+Q++VV S+ +AA
Sbjct: 603 LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 661
Query: 1395 TEILKTHDRILSARCVPHVIPVYPDQIH-FSMAW-VDCSDQWRYLRTLCRTELFSAKAIA 1222
EILKTHDR+LS R VPH +P +I+ S+ W V+C+ W+ LRT+CR ELFS K +
Sbjct: 662 MEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVME 721
Query: 1221 SQASVRDKKTNELVEFLAKKEGKLVNIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGM 1042
SQA V +KK E+V F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++++DGGM
Sbjct: 722 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGM 781
Query: 1041 KGLLRRFAELGSTPNLADFYPILSGLDLQGITKKSKEMFGQIIGVFSVITKQRREDKGND 862
KGL+R+ + PNL DFY I SGLDLQG+ KK+KE+ +I ++ + ++RRE +D
Sbjct: 782 KGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 841
Query: 861 VSRQRXXXXXXXXXXXXXDQINYLIQELFLAGADTSSSTVEWAMTELIKNPKAMRKVCAE 682
S+Q DQIN L EL AGADTSSST+EWAM ELIK+P++M+KV E
Sbjct: 842 PSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEE 900
Query: 681 LERETSGHIVKESELPHLPYLHSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQV 502
L RE S ++ K S+LPHLPYL + V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+
Sbjct: 901 LAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQI 960
Query: 501 LVNAWAIGRDPSTWEDPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAATQVT 322
VNAWAIGRDP WEDPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA +
Sbjct: 961 WVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLP 1020
Query: 321 LILACLINCFDWLLPHDMLLSELDMDDKIGITSQKKKPLVLIPKVTK 181
LILA L + FDW LP+ ELDM+DK G+T QK++PL++IPK K
Sbjct: 1021 LILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKKRK 1067
>ref|XP_002298058.1| cytochrome P450 [Populus trichocarpa] gi|222845316|gb|EEE82863.1|
cytochrome P450 [Populus trichocarpa]
Length = 507
Score = 522 bits (1345), Expect = e-145
Identities = 263/471 (55%), Positives = 338/471 (71%), Gaps = 13/471 (2%)
Frame = -1
Query: 1554 PPGPFPWPVIGNILNMRNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAATEILKTH 1375
PPGP+PWP++GNIL + +KPHI+L AK +GP+ SLRLGTQLVVVGSSQAAA ILKTH
Sbjct: 40 PPGPYPWPILGNILQLGDKPHITLTHFAKIHGPIFSLRLGTQLVVVGSSQAAAIAILKTH 99
Query: 1374 DRILSARCVPHVIPVYPDQIH-FSMAWV-DCSDQWRYLRTLCRTELFSAKAIASQASVRD 1201
DRILS R VPH+ P +++ S+ WV +C+++WRYLRT+C++ELFS KA+ SQA +R+
Sbjct: 100 DRILSGRHVPHMAPSKSSELNKLSLGWVVECNERWRYLRTICKSELFSLKALESQACIRE 159
Query: 1200 KKTNELVEFLAKKEGKLVNIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGMKGLLRRF 1021
+K E++ F+ K EGK+V I EV ATVFNMLSNI VS+D +SLE ESEDGGM +L+
Sbjct: 160 RKAKEMIGFINKMEGKVVKIREVATATVFNMLSNILVSRDLVSLEHESEDGGMSSVLKDI 219
Query: 1020 AELGSTPNLADFYPILSGLDLQGITKKSKEMFGQIIGVFSVITKQRRED-----KGNDVS 856
A L STPN++DFYPIL LDLQG+ KK+ E+ + + I ++RRE G D S
Sbjct: 220 ARLASTPNISDFYPILGPLDLQGLRKKTMELHRRSFNMCEAIIQERREGGEGKRDGPDAS 279
Query: 855 RQRXXXXXXXXXXXXXDQINYLIQELFLAGADTSSSTVEWAMTELIKNPKAMRKVCAEL- 679
R+R DQI+ L+ EL AG DTSSST+EW M ELIKNP+ ++KV E+
Sbjct: 280 RRRDFLDALILNGSSDDQIDILLMELLSAGTDTSSSTIEWTMAELIKNPRCLKKVQEEIA 339
Query: 678 -----ERETSGHIVKESELPHLPYLHSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPK 514
R+T KES LP L YL + V ETLRLHPP PFLLPHRA+++C+V+NYTIPK
Sbjct: 340 NVINMNRDTG---FKESHLPQLTYLQACVKETLRLHPPGPFLLPHRAIDSCQVMNYTIPK 396
Query: 513 DSQVLVNAWAIGRDPSTWEDPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAA 334
++QVLVN WAIGRDP +WE+P+ F PERFL S LD ++F+FIPFG+GRRICPGLPMAA
Sbjct: 397 NTQVLVNYWAIGRDPKSWEEPVVFNPERFLSSNLDFKGNDFEFIPFGSGRRICPGLPMAA 456
Query: 333 TQVTLILACLINCFDWLLPHDMLLSELDMDDKIGITSQKKKPLVLIPKVTK 181
V LI+A LI FDW LP ++LDM + G+T +K++PL+L+P K
Sbjct: 457 KHVALIIAYLILFFDWSLPCGKNPTDLDMSENYGLTLRKEQPLLLVPTSKK 507