BLASTX nr result
ID: Coptis23_contig00004743
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00004743
(1483 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002283479.1| PREDICTED: E3 ubiquitin-protein ligase HERC2... 451 e-180
ref|XP_002329325.1| predicted protein [Populus trichocarpa] gi|2... 439 e-174
ref|XP_002516745.1| Ran GTPase binding protein, putative [Ricinu... 438 e-173
ref|XP_004141502.1| PREDICTED: LOW QUALITY PROTEIN: probable E3 ... 432 e-170
ref|XP_004156186.1| PREDICTED: LOW QUALITY PROTEIN: probable E3 ... 432 e-170
>ref|XP_002283479.1| PREDICTED: E3 ubiquitin-protein ligase HERC2 [Vitis vinifera]
gi|296087299|emb|CBI33673.3| unnamed protein product
[Vitis vinifera]
Length = 395
Score = 451 bits (1159), Expect(2) = e-180
Identities = 211/248 (85%), Positives = 226/248 (91%)
Frame = -1
Query: 1285 MASLNPIILAWGSGEDGQLGIGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKL 1106
MAS I++WGSGEDGQLGIG+NE+KEWVC++K+L +Q + +VV GSRNSLAICEDGKL
Sbjct: 1 MASKPKAIISWGSGEDGQLGIGNNEEKEWVCAVKALAAQNVCSVVAGSRNSLAICEDGKL 60
Query: 1105 FTWGWNQRGTLGHPPETKTENIPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGNE 926
FTWGWNQRGTLGHPPETKTENIPSQVKALAN KIVQAAIGGWHCLAVDD+G+AYAWGGNE
Sbjct: 61 FTWGWNQRGTLGHPPETKTENIPSQVKALANAKIVQAAIGGWHCLAVDDQGRAYAWGGNE 120
Query: 925 YGQCGEEPERKXXXXXXXXXDIVIPQRCAPKLSVRQVAAGGTHSVVLTNEGHLWTWGQPW 746
YGQCGEEPERK DI IPQRCA KLSVRQVAAGGTHSVVLT EGH+WTWGQPW
Sbjct: 121 YGQCGEEPERKDDTGRPLRRDITIPQRCASKLSVRQVAAGGTHSVVLTREGHVWTWGQPW 180
Query: 745 PPGDIKQISTPVRVQGLEKVKLIAVGAFHNLALLEDGMLWAWGNNEYGQLGTGDTQPRSQ 566
PPGDIKQISTPVRVQGLEKV+LIAVGAFHNLALLEDG+LWAWGNNEYGQLGTGDTQPRSQ
Sbjct: 181 PPGDIKQISTPVRVQGLEKVRLIAVGAFHNLALLEDGILWAWGNNEYGQLGTGDTQPRSQ 240
Query: 565 PIPVQGLS 542
PI VQGLS
Sbjct: 241 PIAVQGLS 248
Score = 208 bits (529), Expect(2) = e-180
Identities = 100/125 (80%), Positives = 106/125 (84%), Gaps = 1/125 (0%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGDDKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSFG 246
G VYGWGRGEHGRLGFGDDKSSKMVPQ+VQLLV E+IVQ+SCGGTHSVALTRDGRMFSFG
Sbjct: 269 GGVYGWGRGEHGRLGFGDDKSSKMVPQKVQLLVEENIVQISCGGTHSVALTRDGRMFSFG 328
Query: 245 RGDHGRLGYGRKGTTGNPTEVLINIPQPRNLSNIGT-GQWCAKLVACGGRHTLAIATWQT 69
RGDHGRLGYGRK TTG+P EV IN+P NLS G G WC+KLVACGGRHTLAI W
Sbjct: 329 RGDHGRLGYGRKVTTGHPMEVPINLPPATNLSGSGAGGHWCSKLVACGGRHTLAIVEWLA 388
Query: 68 EELEQ 54
E EQ
Sbjct: 389 HEPEQ 393
Score = 89.4 bits (220), Expect = 2e-15
Identities = 51/159 (32%), Positives = 89/159 (55%), Gaps = 2/159 (1%)
Frame = -1
Query: 1222 GDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQRGTLGHPPETKTEN 1043
GD + ++ LE ++ AV G+ ++LA+ EDG L+ WG N+ G LG +T+ +
Sbjct: 183 GDIKQISTPVRVQGLEKVRLIAV--GAFHNLALLEDGILWAWGNNEYGQLG-TGDTQPRS 239
Query: 1042 IPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEEPERKXXXXXXXXXD 863
P V+ L+++ +V A GGWH A+ D G Y WG E+G+ G ++
Sbjct: 240 QPIAVQGLSDLTLVDIAAGGWHSTALTDDGGVYGWGRGEHGRLGFGDDKSSK-------- 291
Query: 862 IVIPQRCAPKL--SVRQVAAGGTHSVVLTNEGHLWTWGQ 752
++PQ+ + ++ Q++ GGTHSV LT +G ++++G+
Sbjct: 292 -MVPQKVQLLVEENIVQISCGGTHSVALTRDGRMFSFGR 329
Score = 80.9 bits (198), Expect = 8e-13
Identities = 36/119 (30%), Positives = 67/119 (56%)
Frame = -1
Query: 1267 IILAWGSGEDGQLGIGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLFTWGWN 1088
I+ AWG+ E GQLG GD + + +++ L + + G +S A+ +DG ++ WG
Sbjct: 218 ILWAWGNNEYGQLGTGDTQPRSQPIAVQGLSDLTLVDIAAGGWHSTALTDDGGVYGWGRG 277
Query: 1087 QRGTLGHPPETKTENIPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCG 911
+ G LG + ++ +P +V+ L IVQ + GG H +A+ G+ +++G ++G+ G
Sbjct: 278 EHGRLGFGDDKSSKMVPQKVQLLVEENIVQISCGGTHSVALTRDGRMFSFGRGDHGRLG 336
Score = 64.7 bits (156), Expect = 6e-08
Identities = 38/116 (32%), Positives = 61/116 (52%), Gaps = 2/116 (1%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGDDKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSFG 246
G+++ WG + G LG + ++ +P +V+ L IVQ + GG H +A+ GR +++G
Sbjct: 58 GKLFTWGWNQRGTLGHPPETKTENIPSQVKALANAKIVQAAIGGWHCLAVDDQGRAYAWG 117
Query: 245 RGDHGRLGY--GRKGTTGNPTEVLINIPQPRNLSNIGTGQWCAKLVACGGRHTLAI 84
++G+ G RK TG P I IPQ R S + Q VA GG H++ +
Sbjct: 118 GNEYGQCGEEPERKDDTGRPLRRDITIPQ-RCASKLSVRQ-----VAAGGTHSVVL 167
>ref|XP_002329325.1| predicted protein [Populus trichocarpa] gi|222870779|gb|EEF07910.1|
predicted protein [Populus trichocarpa]
Length = 392
Score = 439 bits (1128), Expect(2) = e-174
Identities = 204/241 (84%), Positives = 219/241 (90%)
Frame = -1
Query: 1264 ILAWGSGEDGQLGIGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQ 1085
++AWGSGEDGQLGIG+NE+KEWVC +K+LE K+ +VV GSRNSLAIC+DGKLFTWGWNQ
Sbjct: 7 VIAWGSGEDGQLGIGNNEEKEWVCIVKALEPYKVRSVVAGSRNSLAICDDGKLFTWGWNQ 66
Query: 1084 RGTLGHPPETKTENIPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEE 905
RGTLGHPPETKTENIPSQVKALANV IVQAAIGGWHCLAVDD+G+AYAWGGNEYGQCGEE
Sbjct: 67 RGTLGHPPETKTENIPSQVKALANVNIVQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGEE 126
Query: 904 PERKXXXXXXXXXDIVIPQRCAPKLSVRQVAAGGTHSVVLTNEGHLWTWGQPWPPGDIKQ 725
PERK DIVIPQRCA KL VRQVAAGGTHSVVLT EGH+W+WGQPWPPGDIKQ
Sbjct: 127 PERKDDTGRPLRRDIVIPQRCASKLVVRQVAAGGTHSVVLTREGHVWSWGQPWPPGDIKQ 186
Query: 724 ISTPVRVQGLEKVKLIAVGAFHNLALLEDGMLWAWGNNEYGQLGTGDTQPRSQPIPVQGL 545
IS PVRVQGLE+V+LIAVGAFHNLAL EDG LWAWGNNEYGQLGTGDTQPRSQPI VQGL
Sbjct: 187 ISVPVRVQGLERVRLIAVGAFHNLALQEDGTLWAWGNNEYGQLGTGDTQPRSQPITVQGL 246
Query: 544 S 542
S
Sbjct: 247 S 247
Score = 202 bits (514), Expect(2) = e-174
Identities = 99/121 (81%), Positives = 106/121 (87%), Gaps = 2/121 (1%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGD-DKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSF 249
GEVYGWGRGEHGRLGFGD DKSSKMVPQRV LLVGEDIVQVSCGGTHSVALTRDGRM++F
Sbjct: 268 GEVYGWGRGEHGRLGFGDNDKSSKMVPQRVNLLVGEDIVQVSCGGTHSVALTRDGRMYTF 327
Query: 248 GRGDHGRLGYGRKGTTGNPTEVLINIPQPRNLSNIG-TGQWCAKLVACGGRHTLAIATWQ 72
GRGDHGRLGYGRK TTG P EV I+IP P+NL++ G G+W AKLVA GGRHTLAI W
Sbjct: 328 GRGDHGRLGYGRKVTTGQPMEVPIDIPPPKNLTDSGDAGRWIAKLVASGGRHTLAIVEWH 387
Query: 71 T 69
T
Sbjct: 388 T 388
Score = 100 bits (249), Expect = 1e-18
Identities = 70/253 (27%), Positives = 117/253 (46%), Gaps = 18/253 (7%)
Frame = -1
Query: 1258 AWGSGEDGQLG--------IGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLF 1103
AWG E GQ G G ++ V + + V G +S+ + +G ++
Sbjct: 114 AWGGNEYGQCGEEPERKDDTGRPLRRDIVIPQRCASKLVVRQVAAGGTHSVVLTREGHVW 173
Query: 1102 TWG--WNQRGTLGHPPETKTENIPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGN 929
+WG W P + K ++P +V+ L V+++ A+G +H LA+ + G +AWG N
Sbjct: 174 SWGQPWP-------PGDIKQISVPVRVQGLERVRLI--AVGAFHNLALQEDGTLWAWGNN 224
Query: 928 EYGQCGEEPERKXXXXXXXXXDIVIPQRCAPKLSVRQVAAGGTHSVVLTNEGHLWTWGQP 749
EYGQ G + I + L++ +AAGG HS LT++G ++ WG+
Sbjct: 225 EYGQLGTGDTQPRSQP--------ITVQGLSGLTLVDIAAGGWHSTALTDDGEVYGWGRG 276
Query: 748 ------WPPGDIKQISTPVRVQGL--EKVKLIAVGAFHNLALLEDGMLWAWGNNEYGQLG 593
+ D P RV L E + ++ G H++AL DG ++ +G ++G+LG
Sbjct: 277 EHGRLGFGDNDKSSKMVPQRVNLLVGEDIVQVSCGGTHSVALTRDGRMYTFGRGDHGRLG 336
Query: 592 TGDTQPRSQPIPV 554
G QP+ V
Sbjct: 337 YGRKVTTGQPMEV 349
Score = 92.4 bits (228), Expect = 3e-16
Identities = 65/213 (30%), Positives = 109/213 (51%), Gaps = 19/213 (8%)
Frame = -1
Query: 1189 IKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQRGTLGHPPETKTENIPSQVKALANV 1010
++ LE ++ AV G+ ++LA+ EDG L+ WG N+ G LG +T+ + P V+ L+ +
Sbjct: 193 VQGLERVRLIAV--GAFHNLALQEDGTLWAWGNNEYGQLG-TGDTQPRSQPITVQGLSGL 249
Query: 1009 KIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEEPERKXXXXXXXXXDIVIPQRCAPKL 830
+V A GGWH A+ D G+ Y WG E+G+ G K ++++ +
Sbjct: 250 TLVDIAAGGWHSTALTDDGEVYGWGRGEHGRLGFGDNDKSSKMVPQRVNLLVGE------ 303
Query: 829 SVRQVAAGGTHSVVLTNEGHLWTWGQP--WPPGDIKQIST--PVRVQ------------- 701
+ QV+ GGTHSV LT +G ++T+G+ G ++++T P+ V
Sbjct: 304 DIVQVSCGGTHSVALTRDGRMYTFGRGDHGRLGYGRKVTTGQPMEVPIDIPPPKNLTDSG 363
Query: 700 --GLEKVKLIAVGAFHNLALLEDGMLWAWGNNE 608
G KL+A G H LA++E W G+++
Sbjct: 364 DAGRWIAKLVASGGRHTLAIVE----WHTGDSK 392
Score = 80.5 bits (197), Expect = 1e-12
Identities = 52/185 (28%), Positives = 84/185 (45%), Gaps = 8/185 (4%)
Frame = -1
Query: 1258 AWGSGEDGQLGIGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQRG 1079
AWG+ E GQLG GD + + +++ L + + G +S A+ +DG+++ WG + G
Sbjct: 220 AWGNNEYGQLGTGDTQPRSQPITVQGLSGLTLVDIAAGGWHSTALTDDGEVYGWGRGEHG 279
Query: 1078 TLGHPPETKTEN-IPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEEP 902
LG K+ +P +V L IVQ + GG H +A+ G+ Y +G ++G+ G
Sbjct: 280 RLGFGDNDKSSKMVPQRVNLLVGEDIVQVSCGGTHSVALTRDGRMYTFGRGDHGRLGY-- 337
Query: 901 ERKXXXXXXXXXDIVIP-------QRCAPKLSVRQVAAGGTHSVVLTNEGHLWTWGQPWP 743
RK I IP A + + VA+GG H++ + W
Sbjct: 338 GRKVTTGQPMEVPIDIPPPKNLTDSGDAGRWIAKLVASGGRHTLAIVE----------WH 387
Query: 742 PGDIK 728
GD K
Sbjct: 388 TGDSK 392
Score = 77.4 bits (189), Expect = 9e-12
Identities = 40/114 (35%), Positives = 62/114 (54%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGDDKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSFG 246
G ++ WG E+G+LG GD + P VQ L G +V ++ GG HS ALT DG ++ +G
Sbjct: 216 GTLWAWGNNEYGQLGTGDTQPRSQ-PITVQGLSGLTLVDIAAGGWHSTALTDDGEVYGWG 274
Query: 245 RGDHGRLGYGRKGTTGNPTEVLINIPQPRNLSNIGTGQWCAKLVACGGRHTLAI 84
RG+HGRLG+G + +N+ ++ V+CGG H++A+
Sbjct: 275 RGEHGRLGFGDNDKSSKMVPQRVNLLVGEDIVQ----------VSCGGTHSVAL 318
Score = 62.8 bits (151), Expect = 2e-07
Identities = 38/116 (32%), Positives = 62/116 (53%), Gaps = 2/116 (1%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGDDKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSFG 246
G+++ WG + G LG + ++ +P +V+ L +IVQ + GG H +A+ GR +++G
Sbjct: 57 GKLFTWGWNQRGTLGHPPETKTENIPSQVKALANVNIVQAAIGGWHCLAVDDQGRAYAWG 116
Query: 245 RGDHGRLGY--GRKGTTGNPTEVLINIPQPRNLSNIGTGQWCAKLVACGGRHTLAI 84
++G+ G RK TG P I IPQ R S + Q VA GG H++ +
Sbjct: 117 GNEYGQCGEEPERKDDTGRPLRRDIVIPQ-RCASKLVVRQ-----VAAGGTHSVVL 166
>ref|XP_002516745.1| Ran GTPase binding protein, putative [Ricinus communis]
gi|223544118|gb|EEF45643.1| Ran GTPase binding protein,
putative [Ricinus communis]
Length = 393
Score = 438 bits (1127), Expect(2) = e-173
Identities = 205/241 (85%), Positives = 217/241 (90%)
Frame = -1
Query: 1264 ILAWGSGEDGQLGIGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQ 1085
++AWG+GEDGQLGIG NE+KEWVC + +LE K+ +VV GSRNSLAIC+DGKLFTWGWNQ
Sbjct: 7 LIAWGAGEDGQLGIGINEEKEWVCVVNALEPYKVRSVVAGSRNSLAICDDGKLFTWGWNQ 66
Query: 1084 RGTLGHPPETKTENIPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEE 905
RGTLGHP ETKTENIPSQVKAL NV IVQAAIGGWHCLAVDD+G+AYAWGGNEYGQCGEE
Sbjct: 67 RGTLGHPAETKTENIPSQVKALNNVNIVQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGEE 126
Query: 904 PERKXXXXXXXXXDIVIPQRCAPKLSVRQVAAGGTHSVVLTNEGHLWTWGQPWPPGDIKQ 725
PERK DIVIPQRCAPKL VRQVAAGGTHSVVLT EGH+WTWGQPWPPGDIKQ
Sbjct: 127 PERKDDTGKTLRRDIVIPQRCAPKLVVRQVAAGGTHSVVLTREGHVWTWGQPWPPGDIKQ 186
Query: 724 ISTPVRVQGLEKVKLIAVGAFHNLALLEDGMLWAWGNNEYGQLGTGDTQPRSQPIPVQGL 545
IS PVRVQGLEKVKLIAVGAFHNLAL EDG LWAWGNNEYGQLGTGDTQPRSQPIPVQGL
Sbjct: 187 ISVPVRVQGLEKVKLIAVGAFHNLALQEDGTLWAWGNNEYGQLGTGDTQPRSQPIPVQGL 246
Query: 544 S 542
S
Sbjct: 247 S 247
Score = 197 bits (501), Expect(2) = e-173
Identities = 95/124 (76%), Positives = 104/124 (83%), Gaps = 3/124 (2%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGD-DKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSF 249
GEVYGWGRGEHGRLGFGD DKSSKMVPQ+V LL GEDIVQ+SCGGTHSVALT+DGRMFSF
Sbjct: 268 GEVYGWGRGEHGRLGFGDNDKSSKMVPQKVNLLAGEDIVQLSCGGTHSVALTQDGRMFSF 327
Query: 248 GRGDHGRLGYGRKGTTGNPTEVLINIPQPRNLSNI--GTGQWCAKLVACGGRHTLAIATW 75
GRGDHGRLGYG+K TTG P EV +IP P++LS G W AKLVACGGRHTLAI W
Sbjct: 328 GRGDHGRLGYGKKVTTGQPVEVPTDIPPPKSLSGTEEAEGHWIAKLVACGGRHTLAIVEW 387
Query: 74 QTEE 63
+ +E
Sbjct: 388 KVDE 391
Score = 107 bits (267), Expect = 8e-21
Identities = 72/253 (28%), Positives = 120/253 (47%), Gaps = 18/253 (7%)
Frame = -1
Query: 1258 AWGSGEDGQLG--------IGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLF 1103
AWG E GQ G G ++ V + + V G +S+ + +G ++
Sbjct: 114 AWGGNEYGQCGEEPERKDDTGKTLRRDIVIPQRCAPKLVVRQVAAGGTHSVVLTREGHVW 173
Query: 1102 TWG--WNQRGTLGHPPETKTENIPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGN 929
TWG W P + K ++P +V+ L VK++ A+G +H LA+ + G +AWG N
Sbjct: 174 TWGQPWP-------PGDIKQISVPVRVQGLEKVKLI--AVGAFHNLALQEDGTLWAWGNN 224
Query: 928 EYGQCGEEPERKXXXXXXXXXDIVIPQRCAPKLSVRQVAAGGTHSVVLTNEGHLWTWGQP 749
EYGQ G + IP + L++ +AAGG HS LT++G ++ WG+
Sbjct: 225 EYGQLGTGDTQPRSQP--------IPVQGLSGLTLVDIAAGGWHSTALTDDGEVYGWGRG 276
Query: 748 ------WPPGDIKQISTPVRVQGL--EKVKLIAVGAFHNLALLEDGMLWAWGNNEYGQLG 593
+ D P +V L E + ++ G H++AL +DG ++++G ++G+LG
Sbjct: 277 EHGRLGFGDNDKSSKMVPQKVNLLAGEDIVQLSCGGTHSVALTQDGRMFSFGRGDHGRLG 336
Query: 592 TGDTQPRSQPIPV 554
G QP+ V
Sbjct: 337 YGKKVTTGQPVEV 349
Score = 95.5 bits (236), Expect = 3e-17
Identities = 66/203 (32%), Positives = 101/203 (49%), Gaps = 20/203 (9%)
Frame = -1
Query: 1189 IKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQRGTLGHPPETKTENIPSQVKALANV 1010
++ LE K+ AV G+ ++LA+ EDG L+ WG N+ G LG +T+ + P V+ L+ +
Sbjct: 193 VQGLEKVKLIAV--GAFHNLALQEDGTLWAWGNNEYGQLG-TGDTQPRSQPIPVQGLSGL 249
Query: 1009 KIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEEPERKXXXXXXXXXDIVIPQRCAPKL 830
+V A GGWH A+ D G+ Y WG E+G+ G K +++ +
Sbjct: 250 TLVDIAAGGWHSTALTDDGEVYGWGRGEHGRLGFGDNDKSSKMVPQKVNLLAGE------ 303
Query: 829 SVRQVAAGGTHSVVLTNEGHLW----------------TWGQPWP-PGDI---KQISTPV 710
+ Q++ GGTHSV LT +G ++ T GQP P DI K +S
Sbjct: 304 DIVQLSCGGTHSVALTQDGRMFSFGRGDHGRLGYGKKVTTGQPVEVPTDIPPPKSLSGTE 363
Query: 709 RVQGLEKVKLIAVGAFHNLALLE 641
+G KL+A G H LA++E
Sbjct: 364 EAEGHWIAKLVACGGRHTLAIVE 386
Score = 77.4 bits (189), Expect = 9e-12
Identities = 44/114 (38%), Positives = 65/114 (57%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGDDKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSFG 246
G ++ WG E+G+LG GD + P VQ L G +V ++ GG HS ALT DG ++ +G
Sbjct: 216 GTLWAWGNNEYGQLGTGDTQPRSQ-PIPVQGLSGLTLVDIAAGGWHSTALTDDGEVYGWG 274
Query: 245 RGDHGRLGYGRKGTTGNPTEVLINIPQPRNLSNIGTGQWCAKLVACGGRHTLAI 84
RG+HGRLG+G + +PQ NL G+ +L +CGG H++A+
Sbjct: 275 RGEHGRLGFGDNDKSSK------MVPQKVNLL---AGEDIVQL-SCGGTHSVAL 318
>ref|XP_004141502.1| PREDICTED: LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase
HERC1-like [Cucumis sativus]
Length = 390
Score = 432 bits (1110), Expect(2) = e-170
Identities = 200/241 (82%), Positives = 218/241 (90%)
Frame = -1
Query: 1264 ILAWGSGEDGQLGIGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQ 1085
I+AWGSGEDGQLG+G+NE++EWVC+IK+LESQ + +VV GSRNSLAI EDGKLFTWGWNQ
Sbjct: 7 IIAWGSGEDGQLGLGNNEEREWVCAIKALESQNVCSVVAGSRNSLAITEDGKLFTWGWNQ 66
Query: 1084 RGTLGHPPETKTENIPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEE 905
RGTLGHP TK+EN+PSQVKAL+NVKIVQAAIGGWHCLAVDD+G+AYAWGGNEYGQCGEE
Sbjct: 67 RGTLGHPAGTKSENVPSQVKALSNVKIVQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGEE 126
Query: 904 PERKXXXXXXXXXDIVIPQRCAPKLSVRQVAAGGTHSVVLTNEGHLWTWGQPWPPGDIKQ 725
PE+K DI IPQRCAPKL VRQVAAGGTHSVVLT EGH+WTWGQPWPPGDI Q
Sbjct: 127 PEKKDNTGRPPRRDIAIPQRCAPKLIVRQVAAGGTHSVVLTREGHVWTWGQPWPPGDITQ 186
Query: 724 ISTPVRVQGLEKVKLIAVGAFHNLALLEDGMLWAWGNNEYGQLGTGDTQPRSQPIPVQGL 545
IS PVRVQGL+ V+LIAVGAFHNLAL EDG LWAWGNNEYGQLGTGDTQPRSQPIPVQGL
Sbjct: 187 ISVPVRVQGLDMVRLIAVGAFHNLALQEDGTLWAWGNNEYGQLGTGDTQPRSQPIPVQGL 246
Query: 544 S 542
S
Sbjct: 247 S 247
Score = 196 bits (498), Expect(2) = e-170
Identities = 94/122 (77%), Positives = 104/122 (85%), Gaps = 1/122 (0%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGD-DKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSF 249
GEVYGWGRGEHGRLGFGD DKSSKMVPQ+VQLL GE+IVQ+SCGGTHSVA+T DGRMFSF
Sbjct: 268 GEVYGWGRGEHGRLGFGDNDKSSKMVPQKVQLLSGEEIVQISCGGTHSVAVTSDGRMFSF 327
Query: 248 GRGDHGRLGYGRKGTTGNPTEVLINIPQPRNLSNIGTGQWCAKLVACGGRHTLAIATWQT 69
GRGDHGRLGYGRK TTG P EV I+IP PR + G W AKLVACGGRHTLA+ W++
Sbjct: 328 GRGDHGRLGYGRKVTTGQPEEVPIDIPPPRGRTE--NGHWIAKLVACGGRHTLALVQWKS 385
Query: 68 EE 63
E+
Sbjct: 386 ED 387
Score = 99.8 bits (247), Expect = 2e-18
Identities = 71/251 (28%), Positives = 118/251 (47%), Gaps = 19/251 (7%)
Frame = -1
Query: 1258 AWGSGEDGQLG--------IGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLF 1103
AWG E GQ G G ++ + + V G +S+ + +G ++
Sbjct: 114 AWGGNEYGQCGEEPEKKDNTGRPPRRDIAIPQRCAPKLIVRQVAAGGTHSVVLTREGHVW 173
Query: 1102 TWG--WNQRGTLGHPPETKTE-NIPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGG 932
TWG W PP T+ ++P +V+ L V+++ A+G +H LA+ + G +AWG
Sbjct: 174 TWGQPW--------PPGDITQISVPVRVQGLDMVRLI--AVGAFHNLALQEDGTLWAWGN 223
Query: 931 NEYGQCGEEPERKXXXXXXXXXDIVIPQRCAPKLSVRQVAAGGTHSVVLTNEGHLWTWGQ 752
NEYGQ G + IP + L++ +AAGG HS LT+ G ++ WG+
Sbjct: 224 NEYGQLGTGDTQPRSQP--------IPVQGLSGLTLVDIAAGGWHSTALTDNGEVYGWGR 275
Query: 751 P------WPPGDIKQISTPVRVQGL--EKVKLIAVGAFHNLALLEDGMLWAWGNNEYGQL 596
+ D P +VQ L E++ I+ G H++A+ DG ++++G ++G+L
Sbjct: 276 GEHGRLGFGDNDKSSKMVPQKVQLLSGEEIVQISCGGTHSVAVTSDGRMFSFGRGDHGRL 335
Query: 595 GTGDTQPRSQP 563
G G QP
Sbjct: 336 GYGRKVTTGQP 346
Score = 88.2 bits (217), Expect = 5e-15
Identities = 60/201 (29%), Positives = 102/201 (50%), Gaps = 18/201 (8%)
Frame = -1
Query: 1189 IKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQRGTLGHPPETKTENIPSQVKALANV 1010
++ L+ ++ AV G+ ++LA+ EDG L+ WG N+ G LG +T+ + P V+ L+ +
Sbjct: 193 VQGLDMVRLIAV--GAFHNLALQEDGTLWAWGNNEYGQLG-TGDTQPRSQPIPVQGLSGL 249
Query: 1009 KIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEEPERKXXXXXXXXXDIVIPQRCA--P 836
+V A GGWH A+ D G+ Y WG E+G+ G K ++PQ+
Sbjct: 250 TLVDIAAGGWHSTALTDNGEVYGWGRGEHGRLGFGDNDKSSK--------MVPQKVQLLS 301
Query: 835 KLSVRQVAAGGTHSVVLTNEGHLWTWGQ-------------PWPPGDIKQISTPVRVQ-- 701
+ Q++ GGTHSV +T++G ++++G+ P ++ P R +
Sbjct: 302 GEEIVQISCGGTHSVAVTSDGRMFSFGRGDHGRLGYGRKVTTGQPEEVPIDIPPPRGRTE 361
Query: 700 -GLEKVKLIAVGAFHNLALLE 641
G KL+A G H LAL++
Sbjct: 362 NGHWIAKLVACGGRHTLALVQ 382
Score = 78.2 bits (191), Expect = 5e-12
Identities = 47/163 (28%), Positives = 81/163 (49%), Gaps = 5/163 (3%)
Frame = -1
Query: 1258 AWGSGEDGQLGIGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQRG 1079
AWG+ E GQLG GD + + ++ L + + G +S A+ ++G+++ WG + G
Sbjct: 220 AWGNNEYGQLGTGDTQPRSQPIPVQGLSGLTLVDIAAGGWHSTALTDNGEVYGWGRGEHG 279
Query: 1078 TLGHPPETKTEN-IPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEEP 902
LG K+ +P +V+ L+ +IVQ + GG H +AV G+ +++G ++G+ G
Sbjct: 280 RLGFGDNDKSSKMVPQKVQLLSGEEIVQISCGGTHSVAVTSDGRMFSFGRGDHGRLGY-- 337
Query: 901 ERKXXXXXXXXXDIVIPQRCAPKLS----VRQVAAGGTHSVVL 785
RK I IP + + VA GG H++ L
Sbjct: 338 GRKVTTGQPEEVPIDIPPPRGRTENGHWIAKLVACGGRHTLAL 380
Score = 58.9 bits (141), Expect = 3e-06
Identities = 36/116 (31%), Positives = 58/116 (50%), Gaps = 2/116 (1%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGDDKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSFG 246
G+++ WG + G LG S+ VP +V+ L IVQ + GG H +A+ GR +++G
Sbjct: 57 GKLFTWGWNQRGTLGHPAGTKSENVPSQVKALSNVKIVQAAIGGWHCLAVDDQGRAYAWG 116
Query: 245 RGDHGRLGY--GRKGTTGNPTEVLINIPQPRNLSNIGTGQWCAKLVACGGRHTLAI 84
++G+ G +K TG P I IPQ + + VA GG H++ +
Sbjct: 117 GNEYGQCGEEPEKKDNTGRPPRRDIAIPQR------CAPKLIVRQVAAGGTHSVVL 166
Score = 57.4 bits (137), Expect = 1e-05
Identities = 37/113 (32%), Positives = 61/113 (53%), Gaps = 1/113 (0%)
Frame = -3
Query: 419 VYGWGRGEHGRLGFGDDKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSFGRG 240
+ WG GE G+LG G+++ + V ++ L +++ V G +S+A+T DG++F++G
Sbjct: 7 IIAWGSGEDGQLGLGNNEEREWVCA-IKALESQNVCSVVAGSRNSLAITEDGKLFTWGWN 65
Query: 239 DHGRLGYGRKGTTGNPTEVLINIP-QPRNLSNIGTGQWCAKLVACGGRHTLAI 84
G LG+ G +E N+P Q + LSN+ Q A GG H LA+
Sbjct: 66 QRGTLGH----PAGTKSE---NVPSQVKALSNVKIVQ-----AAIGGWHCLAV 106
>ref|XP_004156186.1| PREDICTED: LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase
HERC1-like [Cucumis sativus]
Length = 390
Score = 432 bits (1110), Expect(2) = e-170
Identities = 200/241 (82%), Positives = 218/241 (90%)
Frame = -1
Query: 1264 ILAWGSGEDGQLGIGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQ 1085
I+AWGSGEDGQLG+G+NE++EWVC+IK+LESQ + +VV GSRNSLAI EDGKLFTWGWNQ
Sbjct: 7 IIAWGSGEDGQLGLGNNEEREWVCAIKALESQNVCSVVAGSRNSLAITEDGKLFTWGWNQ 66
Query: 1084 RGTLGHPPETKTENIPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEE 905
RGTLGHP TK+EN+PSQVKAL+NVKIVQAAIGGWHCLAVDD+G+AYAWGGNEYGQCGEE
Sbjct: 67 RGTLGHPAGTKSENVPSQVKALSNVKIVQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGEE 126
Query: 904 PERKXXXXXXXXXDIVIPQRCAPKLSVRQVAAGGTHSVVLTNEGHLWTWGQPWPPGDIKQ 725
PE+K DI IPQRCAPKL VRQVAAGGTHSVVLT EGH+WTWGQPWPPGDI Q
Sbjct: 127 PEKKDNTGRPPRRDIAIPQRCAPKLIVRQVAAGGTHSVVLTREGHVWTWGQPWPPGDITQ 186
Query: 724 ISTPVRVQGLEKVKLIAVGAFHNLALLEDGMLWAWGNNEYGQLGTGDTQPRSQPIPVQGL 545
IS PVRVQGL+ V+LIAVGAFHNLAL EDG LWAWGNNEYGQLGTGDTQPRSQPIPVQGL
Sbjct: 187 ISVPVRVQGLDMVRLIAVGAFHNLALQEDGTLWAWGNNEYGQLGTGDTQPRSQPIPVQGL 246
Query: 544 S 542
S
Sbjct: 247 S 247
Score = 195 bits (496), Expect(2) = e-170
Identities = 94/122 (77%), Positives = 103/122 (84%), Gaps = 1/122 (0%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGD-DKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSF 249
GEVYGWGRGEHGRLGFGD DKSSKMVPQ+VQLL GE+IVQ+SCGGTHSVA+T DGRMFSF
Sbjct: 268 GEVYGWGRGEHGRLGFGDNDKSSKMVPQKVQLLSGEEIVQISCGGTHSVAVTSDGRMFSF 327
Query: 248 GRGDHGRLGYGRKGTTGNPTEVLINIPQPRNLSNIGTGQWCAKLVACGGRHTLAIATWQT 69
GRGDHGRLGYGRK TTG P EV I+IP PR + G W AKLVACGGRHTLA+ W+
Sbjct: 328 GRGDHGRLGYGRKVTTGQPEEVPIDIPPPRGRTE--NGHWIAKLVACGGRHTLALVQWKP 385
Query: 68 EE 63
E+
Sbjct: 386 ED 387
Score = 99.8 bits (247), Expect = 2e-18
Identities = 71/251 (28%), Positives = 118/251 (47%), Gaps = 19/251 (7%)
Frame = -1
Query: 1258 AWGSGEDGQLG--------IGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLF 1103
AWG E GQ G G ++ + + V G +S+ + +G ++
Sbjct: 114 AWGGNEYGQCGEEPEKKDNTGRPPRRDIAIPQRCAPKLIVRQVAAGGTHSVVLTREGHVW 173
Query: 1102 TWG--WNQRGTLGHPPETKTE-NIPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGG 932
TWG W PP T+ ++P +V+ L V+++ A+G +H LA+ + G +AWG
Sbjct: 174 TWGQPW--------PPGDITQISVPVRVQGLDMVRLI--AVGAFHNLALQEDGTLWAWGN 223
Query: 931 NEYGQCGEEPERKXXXXXXXXXDIVIPQRCAPKLSVRQVAAGGTHSVVLTNEGHLWTWGQ 752
NEYGQ G + IP + L++ +AAGG HS LT+ G ++ WG+
Sbjct: 224 NEYGQLGTGDTQPRSQP--------IPVQGLSGLTLVDIAAGGWHSTALTDNGEVYGWGR 275
Query: 751 P------WPPGDIKQISTPVRVQGL--EKVKLIAVGAFHNLALLEDGMLWAWGNNEYGQL 596
+ D P +VQ L E++ I+ G H++A+ DG ++++G ++G+L
Sbjct: 276 GEHGRLGFGDNDKSSKMVPQKVQLLSGEEIVQISCGGTHSVAVTSDGRMFSFGRGDHGRL 335
Query: 595 GTGDTQPRSQP 563
G G QP
Sbjct: 336 GYGRKVTTGQP 346
Score = 88.2 bits (217), Expect = 5e-15
Identities = 60/201 (29%), Positives = 102/201 (50%), Gaps = 18/201 (8%)
Frame = -1
Query: 1189 IKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQRGTLGHPPETKTENIPSQVKALANV 1010
++ L+ ++ AV G+ ++LA+ EDG L+ WG N+ G LG +T+ + P V+ L+ +
Sbjct: 193 VQGLDMVRLIAV--GAFHNLALQEDGTLWAWGNNEYGQLG-TGDTQPRSQPIPVQGLSGL 249
Query: 1009 KIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEEPERKXXXXXXXXXDIVIPQRCA--P 836
+V A GGWH A+ D G+ Y WG E+G+ G K ++PQ+
Sbjct: 250 TLVDIAAGGWHSTALTDNGEVYGWGRGEHGRLGFGDNDKSSK--------MVPQKVQLLS 301
Query: 835 KLSVRQVAAGGTHSVVLTNEGHLWTWGQ-------------PWPPGDIKQISTPVRVQ-- 701
+ Q++ GGTHSV +T++G ++++G+ P ++ P R +
Sbjct: 302 GEEIVQISCGGTHSVAVTSDGRMFSFGRGDHGRLGYGRKVTTGQPEEVPIDIPPPRGRTE 361
Query: 700 -GLEKVKLIAVGAFHNLALLE 641
G KL+A G H LAL++
Sbjct: 362 NGHWIAKLVACGGRHTLALVQ 382
Score = 78.2 bits (191), Expect = 5e-12
Identities = 47/163 (28%), Positives = 81/163 (49%), Gaps = 5/163 (3%)
Frame = -1
Query: 1258 AWGSGEDGQLGIGDNEDKEWVCSIKSLESQKISAVVGGSRNSLAICEDGKLFTWGWNQRG 1079
AWG+ E GQLG GD + + ++ L + + G +S A+ ++G+++ WG + G
Sbjct: 220 AWGNNEYGQLGTGDTQPRSQPIPVQGLSGLTLVDIAAGGWHSTALTDNGEVYGWGRGEHG 279
Query: 1078 TLGHPPETKTEN-IPSQVKALANVKIVQAAIGGWHCLAVDDKGQAYAWGGNEYGQCGEEP 902
LG K+ +P +V+ L+ +IVQ + GG H +AV G+ +++G ++G+ G
Sbjct: 280 RLGFGDNDKSSKMVPQKVQLLSGEEIVQISCGGTHSVAVTSDGRMFSFGRGDHGRLGY-- 337
Query: 901 ERKXXXXXXXXXDIVIPQRCAPKLS----VRQVAAGGTHSVVL 785
RK I IP + + VA GG H++ L
Sbjct: 338 GRKVTTGQPEEVPIDIPPPRGRTENGHWIAKLVACGGRHTLAL 380
Score = 58.9 bits (141), Expect = 3e-06
Identities = 36/116 (31%), Positives = 58/116 (50%), Gaps = 2/116 (1%)
Frame = -3
Query: 425 GEVYGWGRGEHGRLGFGDDKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSFG 246
G+++ WG + G LG S+ VP +V+ L IVQ + GG H +A+ GR +++G
Sbjct: 57 GKLFTWGWNQRGTLGHPAGTKSENVPSQVKALSNVKIVQAAIGGWHCLAVDDQGRAYAWG 116
Query: 245 RGDHGRLGY--GRKGTTGNPTEVLINIPQPRNLSNIGTGQWCAKLVACGGRHTLAI 84
++G+ G +K TG P I IPQ + + VA GG H++ +
Sbjct: 117 GNEYGQCGEEPEKKDNTGRPPRRDIAIPQR------CAPKLIVRQVAAGGTHSVVL 166
Score = 57.4 bits (137), Expect = 1e-05
Identities = 37/113 (32%), Positives = 61/113 (53%), Gaps = 1/113 (0%)
Frame = -3
Query: 419 VYGWGRGEHGRLGFGDDKSSKMVPQRVQLLVGEDIVQVSCGGTHSVALTRDGRMFSFGRG 240
+ WG GE G+LG G+++ + V ++ L +++ V G +S+A+T DG++F++G
Sbjct: 7 IIAWGSGEDGQLGLGNNEEREWVCA-IKALESQNVCSVVAGSRNSLAITEDGKLFTWGWN 65
Query: 239 DHGRLGYGRKGTTGNPTEVLINIP-QPRNLSNIGTGQWCAKLVACGGRHTLAI 84
G LG+ G +E N+P Q + LSN+ Q A GG H LA+
Sbjct: 66 QRGTLGH----PAGTKSE---NVPSQVKALSNVKIVQ-----AAIGGWHCLAV 106