BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00004698
         (1370 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   660   0.0  
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...   580   e-163
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ...   523   e-146
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]        456   e-126
gb|AAQ01669.1| (R,S)-norcoclaurine 6-O-methyltransferase [Papave...   432   e-119

>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase; Short=6-OMT
            gi|9711914|dbj|BAB08004.1|
            S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  660 bits (1702), Expect = 0.0
 Identities = 323/347 (93%), Positives = 329/347 (94%)
 Frame = -1

Query: 1250 MEMKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQPV 1071
            ME+KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHN+GTSMTLSELSSRLPSQPV
Sbjct: 1    MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPV 60

Query: 1070 NEDALYRVMRYLVHMKLFTKESIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMS 891
            NEDALYRVMRYLVHMKLFTK SIDGELRYGLAPPAK+LVKGWDKCMVGSILAITDKDFM+
Sbjct: 61   NEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMA 120

Query: 890  PWHYLKDGLSGESGTAFEKALGMNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 711
            PWHYLKDGLSGESGTAFEKALG NIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN
Sbjct: 121  PWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 180

Query: 710  IFNXXXXXXXXXXXXXXXVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFI 531
            IFN               VRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFI
Sbjct: 181  IFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFI 240

Query: 530  PKADAIMMKCILHDWDDKECIEILKRCKEAVPIEGGKVIIVDIVLNVQSEHPYTKMRLTL 351
            PKADAIMMKCILHDWDDKECIEILKRCKEAVP++GGKVIIVDIVLNVQSEHPYTKMRLTL
Sbjct: 241  PKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTL 300

Query: 350  DLDMMLNTGGKERTEEEWKNLIHDAGYKGHKITQITAVQSVIEAYPY 210
            DLDMMLNTGGKERTEEEWK LIHDAGYKGHKITQITAVQSVIEAYPY
Sbjct: 301  DLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYPY 347


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
            glaucum]
          Length = 350

 Score =  580 bits (1495), Expect = e-163
 Identities = 282/350 (80%), Positives = 311/350 (88%), Gaps = 3/350 (0%)
 Frame = -1

Query: 1250 MEM-KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQP 1074
            MEM  K+NLSSQAKLWNFIYGFA+SLVLK AVQLDLANIIHN+G+ MTLSELS  LPSQP
Sbjct: 1    MEMINKENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQP 60

Query: 1073 VNEDALYRVMRYLVHMKLFTKESIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFM 894
            VN+DALYRV+RYLVHMKLFTK SIDGELRYGLAPPAKFLVKGWDKCM+G+IL ITDKDFM
Sbjct: 61   VNQDALYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFM 120

Query: 893  SPWHYLKDGL--SGESGTAFEKALGMNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKE 720
            +PWHYLK+G+   G + TAFEKALG NIW YMAEHPEKNQLFNE MAND+RLIMSALVKE
Sbjct: 121  APWHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKE 180

Query: 719  CGNIFNXXXXXXXXXXXXXXXVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMF 540
            C ++F+               VRNIA AFPHIKCTVYDLPHVIADSPGY+E++ + GDMF
Sbjct: 181  CSSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMF 240

Query: 539  KFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPIEGGKVIIVDIVLNVQSEHPYTKMR 360
            K+IP ADAIMMKCILHDWDDKECIEILKRCK+AVP +GGKVII+DI+L+V+SEHPYTKMR
Sbjct: 241  KYIPNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMR 300

Query: 359  LTLDLDMMLNTGGKERTEEEWKNLIHDAGYKGHKITQITAVQSVIEAYPY 210
            LTLDLDMMLNTGGKERTEEEWK LIHDAGYKG+KIT I+AVQSVIEAYPY
Sbjct: 301  LTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYPY 350


>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum flavum]
          Length = 346

 Score =  523 bits (1348), Expect = e-146
 Identities = 257/345 (74%), Positives = 295/345 (85%), Gaps = 3/345 (0%)
 Frame = -1

Query: 1235 DNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQPVNEDAL 1056
            ++LSSQAKLWN+IYGFA+SLVLKCAVQLDLANIIH++GT MTLSELSSRLPSQPVN+DAL
Sbjct: 2    EDLSSQAKLWNYIYGFADSLVLKCAVQLDLANIIHDHGTPMTLSELSSRLPSQPVNQDAL 61

Query: 1055 YRVMRYLVHMKLFTKESIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMSPWHYL 876
            YRVMRYLVH+KLFTK SIDGELRYGLAPPAKFLVKGWD CM+GSIL + D DFM+P+HY+
Sbjct: 62   YRVMRYLVHIKLFTKSSIDGELRYGLAPPAKFLVKGWDNCMIGSILLMVDTDFMAPYHYI 121

Query: 875  KDGL--SGESGTAFEKALGMNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIFN 702
            KD +   G S TAFEKA G NIW Y+AEHPEKNQLFNE MAN+SRL MS L+K+C + F 
Sbjct: 122  KDEILNDGSSITAFEKASGTNIWEYLAEHPEKNQLFNEGMANESRLTMSGLMKDCSSTFE 181

Query: 701  XXXXXXXXXXXXXXXVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIPKA 522
                           V+ IA AFP+IK TVYDLPHVIAD+PG +E++ V GDMFK+IP A
Sbjct: 182  GLTTLVDVGGGTGTTVKYIAKAFPNIKYTVYDLPHVIADAPGDTEINFVEGDMFKYIPNA 241

Query: 521  DAIMMKCILHDWDDKECIEILKRCKEAVPIEGGKVIIVDIVLNVQSEHPYTKMRLTLDLD 342
            DAI+MK +LHDWDD ECIEILKRCK+AVP +GGKVII+D+VLNV+SEHPYTKMRLTLDLD
Sbjct: 242  DAILMKGVLHDWDDNECIEILKRCKDAVPRDGGKVIIIDVVLNVKSEHPYTKMRLTLDLD 301

Query: 341  MMLNTGGKERTEEEWKNLIH-DAGYKGHKITQITAVQSVIEAYPY 210
            MMLN  GKERTEE+WK LI+ DAGYKG+KITQI+A+QSVIEAYPY
Sbjct: 302  MMLNNKGKERTEEDWKKLIYDDAGYKGYKITQISALQSVIEAYPY 346


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score =  456 bits (1173), Expect = e-126
 Identities = 225/352 (63%), Positives = 279/352 (79%), Gaps = 5/352 (1%)
 Frame = -1

Query: 1250 MEMK---KDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPS 1080
            MEM+   K + ++QAK+W  IYGFAESL+LK AV+L++A+ IHN+G  MTLSEL+S+LP 
Sbjct: 1    MEMEGADKIDQATQAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPV 60

Query: 1079 QPVNEDALYRVMRYLVHMKLFTKESIDGE--LRYGLAPPAKFLVKGWDKCMVGSILAITD 906
            QPV+ D LYRVMRYLVHMKLF K+  + E  ++Y L PPAKFL+KGW+K MV +IL I D
Sbjct: 61   QPVDLDRLYRVMRYLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLIND 120

Query: 905  KDFMSPWHYLKDGLSGESGTAFEKALGMNIWGYMAEHPEKNQLFNEAMANDSRLIMSALV 726
            KDFMSPWH+LK+GL   S  AF+KALG +IW YM+ +P+KN++FNE MA DSRL+ SALV
Sbjct: 121  KDFMSPWHHLKEGLVNNSN-AFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALV 179

Query: 725  KECGNIFNXXXXXXXXXXXXXXXVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGD 546
             ECGNIF                V+ ++ AFPHIKC++YDLPHVI+DSP    +  + G+
Sbjct: 180  NECGNIFQGIQTLVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGE 239

Query: 545  MFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPIEGGKVIIVDIVLNVQSEHPYTK 366
            MFK IP ADAI+MKCILHDW+D ECIEILK+CKEAVP EGGKVIIVDIVL+++S+HPY+K
Sbjct: 240  MFKSIPSADAILMKCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHPYSK 299

Query: 365  MRLTLDLDMMLNTGGKERTEEEWKNLIHDAGYKGHKITQITAVQSVIEAYPY 210
            +RLTLDLDMMLNT GKERTEEEWK LI  AG+  +KITQI+A+QSVIEA+PY
Sbjct: 300  VRLTLDLDMMLNTEGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAFPY 351


>gb|AAQ01669.1| (R,S)-norcoclaurine 6-O-methyltransferase [Papaver somniferum]
          Length = 346

 Score =  432 bits (1112), Expect = e-119
 Identities = 217/347 (62%), Positives = 268/347 (77%), Gaps = 2/347 (0%)
 Frame = -1

Query: 1244 MKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLP-SQPVN 1068
            + K +  +QAK+W  IYGFAESLVLKCAVQL++A  +HNN   M+LSEL+S+LP +QPVN
Sbjct: 4    VSKIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVN 63

Query: 1067 EDALYRVMRYLVHMKLFTKESIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMSP 888
            ED L+R+MRYLVHM+LF  ++     +Y LAPPAK+L++GW+K MV SIL I DKDF++P
Sbjct: 64   EDRLFRIMRYLVHMELFKIDATTQ--KYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 121

Query: 887  WHYLKDGLSGESGTAFEKALGMNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNI 708
            WH+L DGL+G    AFEKALG +IW YM+ +PEKNQLFN AMA D+RL+ SAL  EC +I
Sbjct: 122  WHHLGDGLTGNCD-AFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKSI 180

Query: 707  FNXXXXXXXXXXXXXXXV-RNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFI 531
            F+                 + I+ AFP IKCT+YDLPHVIADSP    +  ++GDMFK I
Sbjct: 181  FSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240

Query: 530  PKADAIMMKCILHDWDDKECIEILKRCKEAVPIEGGKVIIVDIVLNVQSEHPYTKMRLTL 351
            P ADAI MKCILHDW+D ECI+ILKRCKEA+P +GGKVIIVD+V+++ S HPY K+RLTL
Sbjct: 241  PSADAIFMKCILHDWNDDECIQILKRCKEALP-KGGKVIIVDVVIDMDSTHPYAKIRLTL 299

Query: 350  DLDMMLNTGGKERTEEEWKNLIHDAGYKGHKITQITAVQSVIEAYPY 210
            DLDMMLNTGGKERT+EEWK L   AG+  HK+TQI+AVQSVIEAYPY
Sbjct: 300  DLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYPY 346