BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00004547
         (746 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       382   e-104
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       288   1e-75
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   280   2e-73
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   274   2e-71
gb|ADB89214.1| stylopine synthase [Papaver somniferum]                243   4e-62

>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  382 bits (980), Expect = e-104
 Identities = 201/239 (84%), Positives = 207/239 (86%), Gaps = 5/239 (2%)
 Frame = -2

Query: 703 MEGSFLWLIGSTVAIVLAITTIVRMXXXXXXXXXXXK----RLPIIGNXXXXXXXXXHVT 536
           MEGSFLWLIGSTVAIVL ITTIVRM                RLPIIGN         HVT
Sbjct: 1   MEGSFLWLIGSTVAIVLVITTIVRMSSSSSSSKKKWPSGPKRLPIIGNLHLLGGDLLHVT 60

Query: 535 LAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVLVSKSAEYGQRDTPEIFKIFT-GQND 359
           LAKLAKVHGSVMTIWIG WRPIIVISDINSAWEVLVSKSAEYGQRDTP+IFK  T GQN+
Sbjct: 61  LAKLAKVHGSVMTIWIGRWRPIIVISDINSAWEVLVSKSAEYGQRDTPDIFKTLTVGQNN 120

Query: 358 ISLSDICPFWHNVRKGLQNGALSPLSVAAQTQFREEDVKRLIHEMSREALNKNGIIKPLD 179
           IS+SDI PFWHNVRKGLQNGALSPL+VAAQTQFREEDVKRLIHEMSREALNKNGIIKPLD
Sbjct: 121 ISMSDIGPFWHNVRKGLQNGALSPLNVAAQTQFREEDVKRLIHEMSREALNKNGIIKPLD 180

Query: 178 HMKKNTARLLGRLIFGPTFDNDKFVESMLYEIEDVLRISGYAGLTEAFYYAKYLPSQKK 2
           HMKKNTARLLGRLIFGPTFDNDKFVES+LYEIEDVLR+SGYA LTEAFYYAKYLPSQKK
Sbjct: 181 HMKKNTARLLGRLIFGPTFDNDKFVESVLYEIEDVLRLSGYARLTEAFYYAKYLPSQKK 239


>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  288 bits (736), Expect = 1e-75
 Identities = 152/254 (59%), Positives = 187/254 (73%), Gaps = 10/254 (3%)
 Frame = -2

Query: 733 LHSIVTISTEMEGSFLWLIGSTVAIVLAITTIVRMXXXXXXXXXXXK------RLPIIGN 572
           L ++V +  EMEGS L L G+T+AI+L I+TIVR+                  +LPIIGN
Sbjct: 3   LRNMVAMVAEMEGSLL-LAGATLAILLTISTIVRITSSPSSSFSQMTWPSGPKKLPIIGN 61

Query: 571 XXXXXXXXXHVTLAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVLVSKSAEYGQRDTP 392
                    HVTLAKLAKVHG VMTIW+GSW PIIVISDINSAWEVLV+KSA+YG R+  
Sbjct: 62  LHQLRGELLHVTLAKLAKVHGGVMTIWMGSWVPIIVISDINSAWEVLVNKSADYGAREVT 121

Query: 391 EIFKIFTGQ----NDISLSDICPFWHNVRKGLQNGALSPLSVAAQTQFREEDVKRLIHEM 224
            + ++        ++IS SD+ P WHN+RKGLQ+GALSPLSVA+QTQ RE D+KR++HEM
Sbjct: 122 YLHELLRDSAMHPHNISSSDVGPLWHNIRKGLQSGALSPLSVASQTQHRERDMKRVVHEM 181

Query: 223 SREALNKNGIIKPLDHMKKNTARLLGRLIFGPTFDNDKFVESMLYEIEDVLRISGYAGLT 44
           SRE +N NG IKPL H+K +TARLLGRLIFGP+F++ KF+ESMLYEIED +R S Y  LT
Sbjct: 182 SREIVNNNGTIKPLVHIKNSTARLLGRLIFGPSFNDSKFIESMLYEIEDFIRTSSYTSLT 241

Query: 43  EAFYYAKYLPSQKK 2
            +FYYAKYLPSQ+K
Sbjct: 242 NSFYYAKYLPSQRK 255


>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
           Full=(S)-tetrahydrocolumbamine oxidase
           (methylenedioxy-bridge-forming); AltName:
           Full=Cytochrome P450 719A1 gi|15822820|dbj|BAB68769.1|
           methylenedioxy bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  280 bits (717), Expect = 2e-73
 Identities = 149/233 (63%), Positives = 172/233 (73%), Gaps = 6/233 (2%)
 Frame = -2

Query: 682 LIGSTVAIVLAITTIVRMXXXXXXXXXXXK-----RLPIIGNXXXXXXXXXHVTLAKLAK 518
           L+ +TVAIV A TTI+R+                 +LPIIGN         HV LAKLAK
Sbjct: 7   LVCATVAIVFATTTIIRILFSSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVALAKLAK 66

Query: 517 VHGSVMTIWIGSWRPIIVISDINSAWEVLVSKSAEYGQRDTPEIFKIFTGQ-NDISLSDI 341
           VHGSVMTIWIGSWRP+IVISDI  AWEVLV+KSA+YG RD PEI KI +   + IS SD 
Sbjct: 67  VHGSVMTIWIGSWRPVIVISDIEKAWEVLVNKSADYGARDMPEITKIASASWHTISTSDA 126

Query: 340 CPFWHNVRKGLQNGALSPLSVAAQTQFREEDVKRLIHEMSREALNKNGIIKPLDHMKKNT 161
             FW NVRKGLQ+GA+ PL+VAAQ Q++E D+KRLI  MS EA N NGI+KPLDH+KKNT
Sbjct: 127 GSFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLDHIKKNT 186

Query: 160 ARLLGRLIFGPTFDNDKFVESMLYEIEDVLRISGYAGLTEAFYYAKYLPSQKK 2
            RLL RLIFG  FD++KF+ESM YEIED++RISGYA L EAFYYAKYLPS KK
Sbjct: 187 VRLLTRLIFGQAFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKK 239


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  274 bits (700), Expect = 2e-71
 Identities = 143/240 (59%), Positives = 175/240 (72%), Gaps = 6/240 (2%)
 Frame = -2

Query: 703 MEGSFLWLIGSTVAIVLAITTIVRMXXXXXXXXXXXK-----RLPIIGNXXXXXXXXXHV 539
           ME   LWL+ +T+A+V+AIT ++ M                 +LPIIGN         HV
Sbjct: 1   MELVSLWLVSATLALVVAITILIPMVTSSSSFSQMKWPTGPNKLPIIGNLHQLGGDVLHV 60

Query: 538 TLAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVLVSKSAEYGQRDTPEIFKIFTGQ-N 362
            LAKLA+VHGSVMT+WIG+WRPIIVISDI+ AWEVLV+KS++YG RD PEI KI +   +
Sbjct: 61  ALAKLAQVHGSVMTVWIGNWRPIIVISDIDKAWEVLVNKSSDYGARDMPEITKIASASWH 120

Query: 361 DISLSDICPFWHNVRKGLQNGALSPLSVAAQTQFREEDVKRLIHEMSREALNKNGIIKPL 182
            IS SD   FW  VRKGLQ+GA+ PL++AAQ+QF+E D+KRLI  +  EA   N I+KPL
Sbjct: 121 TISTSDAGAFWQTVRKGLQSGAMGPLNIAAQSQFQERDMKRLIQALRDEAAQNNNIVKPL 180

Query: 181 DHMKKNTARLLGRLIFGPTFDNDKFVESMLYEIEDVLRISGYAGLTEAFYYAKYLPSQKK 2
           DH+KKNT RLL RLIFG TFD+DKFVESM +EI+D++RISGYA L EAFYYAKYLPS KK
Sbjct: 181 DHIKKNTVRLLTRLIFGQTFDDDKFVESMHFEIDDIIRISGYARLAEAFYYAKYLPSHKK 240


>gb|ADB89214.1| stylopine synthase [Papaver somniferum]
          Length = 494

 Score =  243 bits (619), Expect = 4e-62
 Identities = 122/233 (52%), Positives = 163/233 (69%), Gaps = 5/233 (2%)
 Frame = -2

Query: 685 WLIGSTVAIVLAITTIV----RMXXXXXXXXXXXKRLPIIGNXXXXXXXXXHVTLAKLAK 518
           W++ ++  +V+ I T++    R            K LPIIGN          V L  LAK
Sbjct: 10  WILTTSTLVVITIITMLMVFKRKKSSTMVWPTGPKTLPIIGNMHVLGGTALQVVLHNLAK 69

Query: 517 VHGSVMTIWIGSWRPIIVISDINSAWEVLVSKSAEYGQRDTPEIFKIFTGQ-NDISLSDI 341
           +HG+VMTIWIGSWRP+IV+SDI+ AWEVLV+KS++Y  RD PEI KI +     IS SD 
Sbjct: 70  IHGNVMTIWIGSWRPVIVVSDIDRAWEVLVNKSSDYSARDMPEITKIISADWKTISTSDS 129

Query: 340 CPFWHNVRKGLQNGALSPLSVAAQTQFREEDVKRLIHEMSREALNKNGIIKPLDHMKKNT 161
            P W N+RKGLQN ALSP ++AAQ+QF+E+D+ ++I  + +EA + NGI+KP+DH+KK T
Sbjct: 130 GPGWSNLRKGLQNVALSPHNLAAQSQFQEKDITKMIQSLKKEAASNNGIVKPIDHLKKAT 189

Query: 160 ARLLGRLIFGPTFDNDKFVESMLYEIEDVLRISGYAGLTEAFYYAKYLPSQKK 2
            RL+ RLIFG  FD+DK+V+ M + IE+++R+SGYA L EAFYYAKYLPS KK
Sbjct: 190 LRLISRLIFGQDFDDDKYVDDMHHAIEELIRVSGYAQLAEAFYYAKYLPSHKK 242