BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00004522
         (1314 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285915.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   650   0.0  
ref|XP_003634474.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   640   0.0  
emb|CBI18888.3| unnamed protein product [Vitis vinifera]              639   0.0  
ref|NP_181956.2| cytoplasmic tRNA 2-thiolation protein 1 [Arabid...   630   e-178
ref|XP_002881955.1| hypothetical protein ARALYDRAFT_483552 [Arab...   630   e-178

>ref|XP_002285915.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 1-like isoform 1
            [Vitis vinifera]
          Length = 356

 Score =  650 bits (1678), Expect = 0.0
 Identities = 316/352 (89%), Positives = 333/352 (94%), Gaps = 1/352 (0%)
 Frame = -1

Query: 1233 EAKPKKTGGRQCSICNQRRPALKRPKTLEQICRECFYAVFEDEIHKVIVDNKLFKSGERI 1054
            E KPKK G  QCSIC+QRR ALKRPKTLEQICRECFYAVFE+EIH+V+VDN+LFK GERI
Sbjct: 5    EVKPKKAGPVQCSICSQRRAALKRPKTLEQICRECFYAVFEEEIHRVVVDNQLFKPGERI 64

Query: 1053 GIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNELQYGLP 874
             IGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNE+QYGLP
Sbjct: 65   AIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLP 124

Query: 873  LKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDIA 694
            LKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAA+LKVDKLATGHNADDIA
Sbjct: 125  LKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAAMLKVDKLATGHNADDIA 184

Query: 693  ETVLLNILRGDIARLGRCTSIVTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECI 514
            ETVLLNILRGDIARL RCTSI+TGEDGPIPRCKPFKYTYEKEIVMYAYFK+LDYFSTECI
Sbjct: 185  ETVLLNILRGDIARLSRCTSIITGEDGPIPRCKPFKYTYEKEIVMYAYFKRLDYFSTECI 244

Query: 513  YSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRISNQTKMPEQGTCERCGYISSQKW 334
            YSPNAYRGFAREFIKDLERIRPRAILDII+SGE FRIS   KMPEQGTCERCGYISSQKW
Sbjct: 245  YSPNAYRGFAREFIKDLERIRPRAILDIIRSGEKFRISTSAKMPEQGTCERCGYISSQKW 304

Query: 333  CKACVLLEGLNRGLPRLGIGRTRGL-NGHMTGVEKADGAQSIQSKQCGTLDF 181
            CKACVLLEGLNRGLP+LGIGRTRGL NG     ++++G ++IQSKQCGTLDF
Sbjct: 305  CKACVLLEGLNRGLPKLGIGRTRGLNNGLECDTKQSNGVKNIQSKQCGTLDF 356


>ref|XP_003634474.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 1-like isoform 2
            [Vitis vinifera]
          Length = 363

 Score =  640 bits (1652), Expect = 0.0
 Identities = 314/359 (87%), Positives = 332/359 (92%), Gaps = 8/359 (2%)
 Frame = -1

Query: 1233 EAKPKKTGGRQCSICNQRRPALKRPKTLEQICRECFYAVFEDEIHKVIVDNKLFKSGERI 1054
            E KPKK G  QCSIC+QRR ALKRPKTLEQICRECFYAVFE+EIH+V+VDN+LFK GERI
Sbjct: 5    EVKPKKAGPVQCSICSQRRAALKRPKTLEQICRECFYAVFEEEIHRVVVDNQLFKPGERI 64

Query: 1053 GIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNELQYGLP 874
             IGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNE+QYGLP
Sbjct: 65   AIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLP 124

Query: 873  LKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDIA 694
            LKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAA+LKVDKLATGHNADDIA
Sbjct: 125  LKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAAMLKVDKLATGHNADDIA 184

Query: 693  ETVLLNILRGDIARLGRCTSIVTGEDGPIPRCKPFKYTYEKEIVM-------YAYFKKLD 535
            ETVLLNILRGDIARL RCTSI+TGEDGPIPRCKPFKYTYEKEI+        YAYFK+LD
Sbjct: 185  ETVLLNILRGDIARLSRCTSIITGEDGPIPRCKPFKYTYEKEILQNLNRIYTYAYFKRLD 244

Query: 534  YFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRISNQTKMPEQGTCERCG 355
            YFSTECIYSPNAYRGFAREFIKDLERIRPRAILDII+SGE FRIS   KMPEQGTCERCG
Sbjct: 245  YFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIRSGEKFRISTSAKMPEQGTCERCG 304

Query: 354  YISSQKWCKACVLLEGLNRGLPRLGIGRTRGL-NGHMTGVEKADGAQSIQSKQCGTLDF 181
            YISSQKWCKACVLLEGLNRGLP+LGIGRTRGL NG     ++++G ++IQSKQCGTLDF
Sbjct: 305  YISSQKWCKACVLLEGLNRGLPKLGIGRTRGLNNGLECDTKQSNGVKNIQSKQCGTLDF 363


>emb|CBI18888.3| unnamed protein product [Vitis vinifera]
          Length = 370

 Score =  639 bits (1649), Expect = 0.0
 Identities = 315/366 (86%), Positives = 333/366 (90%), Gaps = 15/366 (4%)
 Frame = -1

Query: 1233 EAKPKKTGGRQCSICNQRRPALKRPKTLEQICRECFYAVFEDEIHKVIVDNKLFKSGERI 1054
            E KPKK G  QCSIC+QRR ALKRPKTLEQICRECFYAVFE+EIH+V+VDN+LFK GERI
Sbjct: 5    EVKPKKAGPVQCSICSQRRAALKRPKTLEQICRECFYAVFEEEIHRVVVDNQLFKPGERI 64

Query: 1053 GIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNELQYGLP 874
             IGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNE+QYGLP
Sbjct: 65   AIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLP 124

Query: 873  LKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDIA 694
            LKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAA+LKVDKLATGHNADDIA
Sbjct: 125  LKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAAMLKVDKLATGHNADDIA 184

Query: 693  ETVLLNILRGDIARLGRCTSIVTGEDGPIPRCKPFKYTYEKEIVM--------------Y 556
            ETVLLNILRGDIARL RCTSI+TGEDGPIPRCKPFKYTYEKEIV+              Y
Sbjct: 185  ETVLLNILRGDIARLSRCTSIITGEDGPIPRCKPFKYTYEKEIVIYPYSFLQNLNRIYTY 244

Query: 555  AYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRISNQTKMPEQ 376
            AYFK+LDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDII+SGE FRIS   KMPEQ
Sbjct: 245  AYFKRLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIRSGEKFRISTSAKMPEQ 304

Query: 375  GTCERCGYISSQKWCKACVLLEGLNRGLPRLGIGRTRGL-NGHMTGVEKADGAQSIQSKQ 199
            GTCERCGYISSQKWCKACVLLEGLNRGLP+LGIGRTRGL NG     ++++G ++IQSKQ
Sbjct: 305  GTCERCGYISSQKWCKACVLLEGLNRGLPKLGIGRTRGLNNGLECDTKQSNGVKNIQSKQ 364

Query: 198  CGTLDF 181
            CGTLDF
Sbjct: 365  CGTLDF 370


>ref|NP_181956.2| cytoplasmic tRNA 2-thiolation protein 1 [Arabidopsis thaliana]
            gi|226711456|sp|O64862.2|CTU1_ARATH RecName:
            Full=Cytoplasmic tRNA 2-thiolation protein 1; AltName:
            Full=Cytoplasmic tRNA adenylyltransferase 1
            gi|26449617|dbj|BAC41934.1| unknown protein [Arabidopsis
            thaliana] gi|30725380|gb|AAP37712.1| At2g44270
            [Arabidopsis thaliana] gi|330255305|gb|AEC10399.1|
            cytoplasmic tRNA 2-thiolation protein 1 [Arabidopsis
            thaliana]
          Length = 355

 Score =  630 bits (1625), Expect = e-178
 Identities = 308/354 (87%), Positives = 325/354 (91%), Gaps = 3/354 (0%)
 Frame = -1

Query: 1233 EAKPKKT-GGRQCSICNQRRPALKRPKTLEQICRECFYAVFEDEIHKVIVDNKLFKSGER 1057
            EAK KK    R C +CN RRP LKRPKTL+QICRECFY VFE+EIH+VIV N+LFKSGER
Sbjct: 2    EAKNKKAVASRLCCLCNLRRPVLKRPKTLQQICRECFYEVFEEEIHQVIVQNRLFKSGER 61

Query: 1056 IGIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNELQYGL 877
            + IGASGGKDSTVLAYVLSELNRRHNYGLDLFLLS+DEGITGYRDDSLETVKRNE+QYGL
Sbjct: 62   VAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSIDEGITGYRDDSLETVKRNEVQYGL 121

Query: 876  PLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDI 697
            PLK+VSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKV+KL TGHNADDI
Sbjct: 122  PLKIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDI 181

Query: 696  AETVLLNILRGDIARLGRCTSIVTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC 517
            AETVLLNILRGDIARL RCTSI TGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC
Sbjct: 182  AETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC 241

Query: 516  IYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRISNQTKMPEQGTCERCGYISSQK 337
            IYSPNAYRGFAREFIKDLERIRPRAILDIIKSGE FRI+  TKMPEQGTCERCGYISSQK
Sbjct: 242  IYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIATTTKMPEQGTCERCGYISSQK 301

Query: 336  WCKACVLLEGLNRGLPRLGIGRTRGLNGHMTGVEKADG--AQSIQSKQCGTLDF 181
            WCKACVLLEGLNRGLP++GIGR RG+NG      K  G  A+SI+SKQCG+LDF
Sbjct: 302  WCKACVLLEGLNRGLPKMGIGRPRGVNGDHNKETKKPGSVAKSIESKQCGSLDF 355


>ref|XP_002881955.1| hypothetical protein ARALYDRAFT_483552 [Arabidopsis lyrata subsp.
            lyrata] gi|297327794|gb|EFH58214.1| hypothetical protein
            ARALYDRAFT_483552 [Arabidopsis lyrata subsp. lyrata]
          Length = 355

 Score =  630 bits (1624), Expect = e-178
 Identities = 308/354 (87%), Positives = 325/354 (91%), Gaps = 3/354 (0%)
 Frame = -1

Query: 1233 EAKPKKT-GGRQCSICNQRRPALKRPKTLEQICRECFYAVFEDEIHKVIVDNKLFKSGER 1057
            EAK KK    R C +CN RRP LKRPKTL+QICRECFY VFE+EIH+VIV N+LFKSGER
Sbjct: 2    EAKSKKAVASRLCCLCNLRRPVLKRPKTLQQICRECFYEVFEEEIHQVIVQNRLFKSGER 61

Query: 1056 IGIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNELQYGL 877
            + IGASGGKDSTVLAYVLSELNRRHNYGLDLFLLS+DEGITGYRDDSLETVKRNE+QYGL
Sbjct: 62   VAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSIDEGITGYRDDSLETVKRNEVQYGL 121

Query: 876  PLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDI 697
            PL++VSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKL TGHNADDI
Sbjct: 122  PLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDI 181

Query: 696  AETVLLNILRGDIARLGRCTSIVTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC 517
            AETVLLNILRGDIARL RCTSI TGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC
Sbjct: 182  AETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC 241

Query: 516  IYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRISNQTKMPEQGTCERCGYISSQK 337
            IYSPNAYRGFAREFIKDLERIRPRAILDIIKSGE FRI+  TKMPEQGTCERCGYISSQK
Sbjct: 242  IYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIATTTKMPEQGTCERCGYISSQK 301

Query: 336  WCKACVLLEGLNRGLPRLGIGRTRGLNGHMTGVEKADG--AQSIQSKQCGTLDF 181
            WCKACVLLEGLNRGLP++GIGR RG+NG      K  G  A+SI+SKQCG+LDF
Sbjct: 302  WCKACVLLEGLNRGLPKMGIGRPRGVNGDHKMETKKPGSVAKSIESKQCGSLDF 355