BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00004148
         (1543 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002534375.1| Ubiquitin ligase SINAT2, putative [Ricinus c...   516   e-144
gb|ABK24533.1| unknown [Picea sitchensis]                             513   e-143
ref|XP_002280243.1| PREDICTED: E3 ubiquitin-protein ligase SINAT...   508   e-141
ref|XP_002308357.1| predicted protein [Populus trichocarpa] gi|2...   506   e-141
ref|NP_191363.1| E3 ubiquitin-protein ligase SINAT2 [Arabidopsis...   503   e-140

>ref|XP_002534375.1| Ubiquitin ligase SINAT2, putative [Ricinus communis]
            gi|223525415|gb|EEF28013.1| Ubiquitin ligase SINAT2,
            putative [Ricinus communis]
          Length = 304

 Score =  516 bits (1328), Expect = e-144
 Identities = 234/304 (76%), Positives = 265/304 (87%), Gaps = 1/304 (0%)
 Frame = +3

Query: 378  MASGSSICVDVSESHPVDTELSMTSSHLELSKPLNTS-GIKAGISSTNGVHELLECPVCT 554
            MA G S C +V ESHP  ++  + ++  E +  +    G K G+ S NGVHELLECPVCT
Sbjct: 1    MAPGGSACKEVLESHPAISDYEIGTAKTENNSTVTKGLGGKQGLYSNNGVHELLECPVCT 60

Query: 555  KSMFPPIHQCPNGHTLCSSCKQRVHNHCPTCRYEMGNIRCLALEKVAESLELPCRHQNLG 734
              M+PPIHQCPNGHTLCS+CK RVHN CPTCRY++GNIRCLALEKVAESLELPC++Q+LG
Sbjct: 61   NLMYPPIHQCPNGHTLCSNCKSRVHNCCPTCRYDLGNIRCLALEKVAESLELPCKYQDLG 120

Query: 735  CADVFPYYSKLKHEQLCRFRPYNCPYAGSECPVTGDIPTLVGHLKDDHKVDMHDGCTFNH 914
            C D+FPYYSKLKHEQ CRFRPY+CPYAGSEC +TGDIPTLV HLKDDHKVDMHDGCTFNH
Sbjct: 121  CHDIFPYYSKLKHEQHCRFRPYSCPYAGSECSITGDIPTLVAHLKDDHKVDMHDGCTFNH 180

Query: 915  RYVKSNPQEVENATWMLTVFNCFGQHFCLHFEAFLSGVAPVYMAFLRFMGDDNDAKNFSY 1094
            RYVKSNP EVENATWMLTVFNCFG+ FCLHFEAF  G+APVYMAFLRFMGDDN+AK FSY
Sbjct: 181  RYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQIGMAPVYMAFLRFMGDDNEAKKFSY 240

Query: 1095 SLEVGGNGRKLTWQGVPRSIRDSHKKVRDSYDGLVIHRNMALFFSGGNRQELKLRVTGRI 1274
            SLEVGGNGRKL WQG+PRSIRDSH+KVRDS DGL+I R++AL+FSGG+RQELKLR+TGRI
Sbjct: 241  SLEVGGNGRKLIWQGIPRSIRDSHRKVRDSQDGLIIQRSLALYFSGGDRQELKLRITGRI 300

Query: 1275 WKEQ 1286
            WKE+
Sbjct: 301  WKEE 304


>gb|ABK24533.1| unknown [Picea sitchensis]
          Length = 306

 Score =  513 bits (1320), Expect = e-143
 Identities = 235/306 (76%), Positives = 263/306 (85%), Gaps = 3/306 (0%)
 Frame = +3

Query: 378  MASGSSICVDVSESHPVDTELSMTSSHLELSKPLNTSGI---KAGISSTNGVHELLECPV 548
            M+ GS  C ++SE   +D+ + +    +++  P   S     K GIS TNGVHELL+CP+
Sbjct: 1    MSQGSD-CTEISELGTLDSVIGIARGDIDIVSPPKASTASSGKGGISLTNGVHELLDCPI 59

Query: 549  CTKSMFPPIHQCPNGHTLCSSCKQRVHNHCPTCRYEMGNIRCLALEKVAESLELPCRHQN 728
            C+ SM+PPIHQCPNGHT+CSSCK RVHN CPTCR+E+GNIRCLALEKVAESLELPCR+Q 
Sbjct: 60   CSNSMYPPIHQCPNGHTICSSCKLRVHNRCPTCRHELGNIRCLALEKVAESLELPCRYQK 119

Query: 729  LGCADVFPYYSKLKHEQLCRFRPYNCPYAGSECPVTGDIPTLVGHLKDDHKVDMHDGCTF 908
            LGC D+FPYYSKLKHEQ C FRPY+CPYAGSEC VTGDIP LV HL+DDHKVDMH+GCTF
Sbjct: 120  LGCHDIFPYYSKLKHEQQCMFRPYSCPYAGSECSVTGDIPALVTHLRDDHKVDMHNGCTF 179

Query: 909  NHRYVKSNPQEVENATWMLTVFNCFGQHFCLHFEAFLSGVAPVYMAFLRFMGDDNDAKNF 1088
            NHRYVK NPQEVENATWMLTVFNCFGQ FCLHFEAF  G+APVYMAFLRFMGDDN+AKNF
Sbjct: 180  NHRYVKPNPQEVENATWMLTVFNCFGQQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKNF 239

Query: 1089 SYSLEVGGNGRKLTWQGVPRSIRDSHKKVRDSYDGLVIHRNMALFFSGGNRQELKLRVTG 1268
             YSLEVG NGRKL WQG+PRSIRDSHKKVRDS+DGL+I RNMALFFSGG+RQELKLRVTG
Sbjct: 240  GYSLEVGANGRKLCWQGIPRSIRDSHKKVRDSHDGLIIQRNMALFFSGGDRQELKLRVTG 299

Query: 1269 RIWKEQ 1286
            RIWKEQ
Sbjct: 300  RIWKEQ 305


>ref|XP_002280243.1| PREDICTED: E3 ubiquitin-protein ligase SINAT2 isoform 1 [Vitis
            vinifera] gi|4584257|emb|CAB40578.1| SINA2p [Vitis
            vinifera] gi|297739816|emb|CBI29998.3| unnamed protein
            product [Vitis vinifera]
          Length = 315

 Score =  508 bits (1308), Expect = e-141
 Identities = 238/306 (77%), Positives = 263/306 (85%), Gaps = 4/306 (1%)
 Frame = +3

Query: 378  MASGSSICVDVSESHPV--DTELSMTSSHLELSKPLNTSGI--KAGISSTNGVHELLECP 545
            MA G S   +V ESH    D +++MT +   L+    T+G+  K GISS NGVHELLECP
Sbjct: 1    MAPGGSTYKEVLESHSTVTDYDIAMTKADTNLTPAKTTAGLSGKKGISSPNGVHELLECP 60

Query: 546  VCTKSMFPPIHQCPNGHTLCSSCKQRVHNHCPTCRYEMGNIRCLALEKVAESLELPCRHQ 725
            VCT  M+PPI+QCP+GHTLCS+CK RVHN CPTCR+E+G+IRCLALEKVAESLELPCR+Q
Sbjct: 61   VCTSLMYPPIYQCPSGHTLCSNCKSRVHNCCPTCRHELGDIRCLALEKVAESLELPCRYQ 120

Query: 726  NLGCADVFPYYSKLKHEQLCRFRPYNCPYAGSECPVTGDIPTLVGHLKDDHKVDMHDGCT 905
            +LGC D+FPYYSKLKHEQ CRF PYNCPYAG EC VTGDIPTLV HLK DHKVDMHDGCT
Sbjct: 121  SLGCHDIFPYYSKLKHEQQCRFHPYNCPYAGFECSVTGDIPTLVEHLKGDHKVDMHDGCT 180

Query: 906  FNHRYVKSNPQEVENATWMLTVFNCFGQHFCLHFEAFLSGVAPVYMAFLRFMGDDNDAKN 1085
            FNHRYVKSNPQEVENATWMLTVFNCFG+ FCLHFEAF  G APVYMAFLRFMGDDN+AK 
Sbjct: 181  FNHRYVKSNPQEVENATWMLTVFNCFGKQFCLHFEAFQLGTAPVYMAFLRFMGDDNEAKK 240

Query: 1086 FSYSLEVGGNGRKLTWQGVPRSIRDSHKKVRDSYDGLVIHRNMALFFSGGNRQELKLRVT 1265
            FSYSLEVGGN RKL WQGVPRSIRDSH+KVRDS DGL+I RN+AL+FSGG+RQELKLRVT
Sbjct: 241  FSYSLEVGGNSRKLIWQGVPRSIRDSHRKVRDSQDGLIIQRNLALYFSGGDRQELKLRVT 300

Query: 1266 GRIWKE 1283
            GRIWKE
Sbjct: 301  GRIWKE 306


>ref|XP_002308357.1| predicted protein [Populus trichocarpa] gi|222854333|gb|EEE91880.1|
            predicted protein [Populus trichocarpa]
          Length = 307

 Score =  506 bits (1302), Expect = e-141
 Identities = 234/307 (76%), Positives = 260/307 (84%), Gaps = 4/307 (1%)
 Frame = +3

Query: 378  MASGSSICVDVSESHPV----DTELSMTSSHLELSKPLNTSGIKAGISSTNGVHELLECP 545
            MA G S   +V E H      D   S + +++  +K     G K G+ S NGVHELLECP
Sbjct: 1    MAPGGSAFKEVLEFHSTVADCDVANSKSENNITPTKGTVVLGGKHGVYSNNGVHELLECP 60

Query: 546  VCTKSMFPPIHQCPNGHTLCSSCKQRVHNHCPTCRYEMGNIRCLALEKVAESLELPCRHQ 725
            VCT  M+PPIHQCPNGHTLCS+CK RVHN CPTCRY++GNIRCLALEKVAESLELPC+ Q
Sbjct: 61   VCTNLMYPPIHQCPNGHTLCSNCKLRVHNCCPTCRYDLGNIRCLALEKVAESLELPCKFQ 120

Query: 726  NLGCADVFPYYSKLKHEQLCRFRPYNCPYAGSECPVTGDIPTLVGHLKDDHKVDMHDGCT 905
            +LGC D+FPYYSKLKHEQ CRFRPY+CPYAGSEC VTGDIP LV HLKDDHKVDMHDGCT
Sbjct: 121  SLGCLDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPALVSHLKDDHKVDMHDGCT 180

Query: 906  FNHRYVKSNPQEVENATWMLTVFNCFGQHFCLHFEAFLSGVAPVYMAFLRFMGDDNDAKN 1085
            FNHRYVKSNP EVENATWMLTVFNCFG+ FCLHFEAF  G+APVYMAFLRFMGDDN+AK 
Sbjct: 181  FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240

Query: 1086 FSYSLEVGGNGRKLTWQGVPRSIRDSHKKVRDSYDGLVIHRNMALFFSGGNRQELKLRVT 1265
            FSYSLEVGGNGRKL WQG+PRSIRDSH+KVRDS DGL+I RN+AL+FSGG+RQELKLRVT
Sbjct: 241  FSYSLEVGGNGRKLVWQGIPRSIRDSHRKVRDSQDGLIIQRNLALYFSGGDRQELKLRVT 300

Query: 1266 GRIWKEQ 1286
            GR+WKE+
Sbjct: 301  GRVWKEE 307


>ref|NP_191363.1| E3 ubiquitin-protein ligase SINAT2 [Arabidopsis thaliana]
            gi|46577554|sp|Q9M2P4.1|SINA2_ARATH RecName: Full=E3
            ubiquitin-protein ligase SINAT2; AltName: Full=Seven in
            absentia homolog 2 gi|6729547|emb|CAB67632.1| putative
            protein [Arabidopsis thaliana] gi|21593355|gb|AAM65304.1|
            putative RING zinc finger protein [Arabidopsis thaliana]
            gi|105830184|gb|ABF74716.1| At3g58040 [Arabidopsis
            thaliana] gi|332646214|gb|AEE79735.1| E3
            ubiquitin-protein ligase SINAT2 [Arabidopsis thaliana]
          Length = 308

 Score =  503 bits (1294), Expect = e-140
 Identities = 234/308 (75%), Positives = 263/308 (85%), Gaps = 5/308 (1%)
 Frame = +3

Query: 378  MASGSSICVDVSESHPVDTELSMTSSHLELS-----KPLNTSGIKAGISSTNGVHELLEC 542
            MA G S   +V ES+    +  + ++ +E++     KP +    K GI S NGV+ELLEC
Sbjct: 1    MAPGGSALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLEC 60

Query: 543  PVCTKSMFPPIHQCPNGHTLCSSCKQRVHNHCPTCRYEMGNIRCLALEKVAESLELPCRH 722
            PVCT  M+PPIHQCPNGHTLCS+CK RV N CPTCRYE+GNIRCLALEKVAESLE+PCR+
Sbjct: 61   PVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRY 120

Query: 723  QNLGCADVFPYYSKLKHEQLCRFRPYNCPYAGSECPVTGDIPTLVGHLKDDHKVDMHDGC 902
            QNLGC D+FPYYSKLKHEQ CRFRPY CPYAGSEC VTGDIPTLV HLKDDHKVDMHDGC
Sbjct: 121  QNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGC 180

Query: 903  TFNHRYVKSNPQEVENATWMLTVFNCFGQHFCLHFEAFLSGVAPVYMAFLRFMGDDNDAK 1082
            TFNHRYVKSNP EVENATWMLTVFNCFG+ FCLHFEAF  G+APVYMAFLRFMGD+N+AK
Sbjct: 181  TFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAK 240

Query: 1083 NFSYSLEVGGNGRKLTWQGVPRSIRDSHKKVRDSYDGLVIHRNMALFFSGGNRQELKLRV 1262
             FSYSLEVG +GRKLTWQG+PRSIRDSH+KVRDS DGL+I RN+AL+FSGG+RQELKLRV
Sbjct: 241  KFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRV 300

Query: 1263 TGRIWKEQ 1286
            TGRIWKE+
Sbjct: 301  TGRIWKEE 308