BLASTX nr result
ID: Coptis23_contig00003763
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00003763
(1829 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ACM43505.1| polyphenoloxidase [Camellia nitidissima] 451 e-124
emb|CAN61652.1| hypothetical protein VITISV_006012 [Vitis vinifera] 451 e-124
sp|P43311.1|PPO_VITVI RecName: Full=Polyphenol oxidase, chloropl... 449 e-123
gb|AAB41022.1| polyphenol oxidase [Vitis vinifera] 443 e-122
gb|AEY78528.1| polyphenol oxidase [Canarium album] 443 e-122
>gb|ACM43505.1| polyphenoloxidase [Camellia nitidissima]
Length = 595
Score = 451 bits (1160), Expect = e-124
Identities = 234/503 (46%), Positives = 318/503 (63%), Gaps = 8/503 (1%)
Frame = +2
Query: 2 PVSPPDLSKCQLATDSANGERVNCCPPYTTANVVDFIPPRKEFLRQRKAAHKLSHDEIRK 181
PV+ PDL+KC A A + NCCPP T ++DF P+ +R R AAH + I K
Sbjct: 97 PVTAPDLAKCGAADLPAGAKPTNCCPPKAT-KIIDFKFPQSNSMRVRPAAHLADAEYIAK 155
Query: 182 FQTAIQKLKELPADDPWNYMQQATIHCTYCNGAFDQVGFDDVLLQIHGSWLFTPWHRYYI 361
F A+ +K LP DDP ++ QQA +HC YC+GA+ QVGF ++ LQ+H SWLF P+HRYY+
Sbjct: 156 FSKALALMKALPEDDPRSFKQQANVHCAYCDGAYHQVGFPELDLQVHNSWLFFPFHRYYL 215
Query: 362 YFWEKILGKLIGDPTFSIPYWNWDAPDGMYMPQPYLDTSSTLYNGNRNLDHY-SALLDLD 538
+F+E+ILG LIGDP+F+IP+WNWD+P+GM MP Y D +S LY+ R+ H L+DLD
Sbjct: 216 HFFERILGSLIGDPSFAIPFWNWDSPNGMQMPAMYADPNSPLYDVLRDAKHQPPTLVDLD 275
Query: 539 YTFGAPNPTPEQEEEIKIKNLRKVYNMFTETIGSPALFLGTPLRAGDAPD-GAGSLENM- 712
Y P T Q+ K NL +Y + S LFLG+P RAGD PD GAGSLEN+
Sbjct: 276 YNGSDPTTTDAQQ---KSSNLNVMYRQMVSSSKSARLFLGSPYRAGDEPDPGAGSLENIP 332
Query: 713 HGTLHQWTGPVAIPR-HDMGNFYTAARDTMFFAHHANVDRLWDTYSDLRGNKVEFNDPDW 889
HG +H W G P DMGNFY+A RD++F+ HHANVDR+W + L G + +F DPDW
Sbjct: 333 HGPVHIWCGDRTQPNLEDMGNFYSAGRDSIFYGHHANVDRMWSIWKTLGGKRQDFTDPDW 392
Query: 890 LEASFIFYDENRKVVRCKVKDCLITENLGYTYQTERHPWKDVK--KRYKKIVRGRKRSAG 1063
L A F+FYDEN ++VR KVKDCL + LGY YQ PW + + R K+ R ++
Sbjct: 393 LNAGFVFYDENAQLVRVKVKDCLDSRKLGYVYQDVDIPWLNTRPAPRVSKLSRKINKAGV 452
Query: 1064 EGISLSPVSEFGSAPRALDTTIRVLVPRPKTTRSKDEKEEASEVLVVDEIKIAHGESIKF 1243
+ +P + P+ LD +RV+VPRPK +RSK EK++ E+LV++ I++ +KF
Sbjct: 453 AMAADTPSTAAEVFPKKLDKAVRVMVPRPKKSRSKKEKDDEEEILVIEGIEVDRDVFVKF 512
Query: 1244 DVYIAKPIEGLVGPELGELAGSFVRVAHSHVKKATESRKAKIELGITNLLDDIEAEASDS 1423
DV+I E ++GP E AGSFV V H H K + K + LGIT LL+++ AE D
Sbjct: 513 DVFINDEHEVVIGPNDTEFAGSFVNVPHKH--KHGKKIKTILRLGITELLEELGAEDDDG 570
Query: 1424 LVVSLVPQH--GDVIIGAVHIEY 1486
++V+LVP++ G V IG V IE+
Sbjct: 571 VLVTLVPKNGAGAVTIGGVKIEF 593
>emb|CAN61652.1| hypothetical protein VITISV_006012 [Vitis vinifera]
Length = 601
Score = 451 bits (1159), Expect = e-124
Identities = 238/506 (47%), Positives = 323/506 (63%), Gaps = 12/506 (2%)
Frame = +2
Query: 2 PVSPPDLSKCQLATDSANGERVNCCPPYTTANVVDF-IPPRKEFLRQRKAAHKLSHDEIR 178
P+SPPDLS+C A A + NCCPP ++ ++DF +PP+ LR R AAH S + I
Sbjct: 99 PISPPDLSECGAADLPAGAQPTNCCPP-VSSKIIDFKLPPKNSPLRIRPAAHLASKEYIA 157
Query: 179 KFQTAIQKLKELPADDPWNYMQQATIHCTYCNGAFDQVGFDDVLLQIHGSWLFTPWHRYY 358
K++ AI+ +K LP DDP ++MQQA +HC YCNGA+ QVGF D+ LQ+H SWLF P+HRYY
Sbjct: 158 KYKKAIELMKALPDDDPRSFMQQADVHCAYCNGAYHQVGFPDLDLQVHNSWLFLPYHRYY 217
Query: 359 IYFWEKILGKLIGDPTFSIPYWNWDAPDGMYMPQPYLDTSSTLYNGNRNLDHY-SALLDL 535
+YF+EKILGKLI DPTF++P+WNWDAP GM MP + D +S LY+ R+ H L+DL
Sbjct: 218 LYFYEKILGKLINDPTFALPFWNWDAPAGMQMPAMFADPNSPLYDKLRDAKHQPPKLIDL 277
Query: 536 DYTFGAPNPTPEQEEEIKIKNLRKVYNMFTETIGSPALFLGTPLRAGDAPD-GAGSLENM 712
DY N T EQ+ NL +Y + + +LF+G RAGD PD G G+LEN+
Sbjct: 278 DYNLTDSNDTNEQQIS---SNLSIMYRQIVSSAKTTSLFMGAAYRAGDEPDPGQGTLENI 334
Query: 713 -HGTLHQWTGPVAIPR-HDMGNFYTAARDTMFFAHHANVDRLWDTYSDLRGNKVEFNDPD 886
HG +H W G P DMGNFY+AARD +FF+HH+NVDR+W + L G + +F DPD
Sbjct: 335 PHGPVHIWCGDRTQPNIEDMGNFYSAARDPIFFSHHSNVDRMWTVWKGLGGKRKDFTDPD 394
Query: 887 WLEASFIFYDENRKVVRCKVKDCLITENLGYTYQTERHPWKDVKKR----YKKIVRGRKR 1054
WL+A F+FYDEN ++VR KV+DCL L Y Y PW + + KK+V K
Sbjct: 395 WLDAGFVFYDENAQLVRVKVRDCLDPTKLRYAYHEVDIPWLKSRPKPRQILKKVVNKLKH 454
Query: 1055 SAGEGISLS-PVSEFGSAPRALDTTIRVLVPRPKTTRSKDEKEEASEVLVVDEIKIAHGE 1231
+G + P S P LD+T+RV VPRP+ +RSK EKE+ EVLV+ I++ +
Sbjct: 455 KSGVANAAEIPSSSSIVFPIKLDSTLRVDVPRPRKSRSKKEKEDEEEVLVIKGIELERDK 514
Query: 1232 SIKFDVYIAKPIEGLVGPELGELAGSFVRVAHSHVKKATESRKAKIELGITNLLDDIEAE 1411
+KFD++I + + P+ E AGSFV V H H + + + +GI+ LL+D+EAE
Sbjct: 515 FVKFDIFINDEDDPVSRPDNTEFAGSFVNVPHQH--SHGKKKNTILRIGISELLEDLEAE 572
Query: 1412 ASDSLVVSLVPQHG-DVI-IGAVHIE 1483
DS+VV+LVP++G DVI IG + IE
Sbjct: 573 DDDSVVVTLVPRYGADVITIGGIEIE 598
>sp|P43311.1|PPO_VITVI RecName: Full=Polyphenol oxidase, chloroplastic; Short=PPO; AltName:
Full=Catechol oxidase; Flags: Precursor
gi|1076272|pir||S52629 catechol oxidase (EC 1.10.3.1)
precursor - grape gi|510234|emb|CAA81798.1| polyphenol
oxidase [Vitis vinifera]
Length = 607
Score = 449 bits (1154), Expect = e-123
Identities = 233/506 (46%), Positives = 317/506 (62%), Gaps = 11/506 (2%)
Frame = +2
Query: 2 PVSPPDLSKCQLATDSANGERVNCCPPYTTANVVDF-IPPRKEFLRQRKAAHKLSHDEIR 178
P+ PD+SKC AT NCCPP TT ++DF +P +R R AAH +S + +
Sbjct: 105 PIQAPDISKCGTATVPDGVTPTNCCPPVTT-KIIDFQLPSSGSPMRTRPAAHLVSKEYLA 163
Query: 179 KFQTAIQKLKELPADDPWNYMQQATIHCTYCNGAFDQVGFDDVLLQIHGSWLFTPWHRYY 358
K++ AI+ K LP DDP ++ QQA +HCTYC GA+DQVG+ D+ LQ+H SWLF P+HRYY
Sbjct: 164 KYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQVGYTDLELQVHASWLFLPFHRYY 223
Query: 359 IYFWEKILGKLIGDPTFSIPYWNWDAPDGMYMPQPYLDTSSTLYNGNRNLDHY-SALLDL 535
+YF E+IL KLI DPTF++PYW WD PDGMYMP Y + S+LY+ RN H ++DL
Sbjct: 224 LYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSPSSLYDEKRNAKHLPPTVIDL 283
Query: 536 DYTFGAPNPTPEQEEEIKIKNLRKVYNMFTETIGSPALFLGTPLRAGDAPD-GAGSLENM 712
DY P ++E+K NL +Y +P LFLG P RAGDA D GAG+LE+
Sbjct: 284 DYDGTEPT---IPDDELKTDNLAIMYKQIVSGATTPKLFLGYPYRAGDAIDPGAGTLEHA 340
Query: 713 -HGTLHQWTGPVAIPRHDMGNFYTAARDTMFFAHHANVDRLWDTYSDLRG-NKVEFNDPD 886
H +H+WTG P DMGNFYTA RD +FF HHANVDR+W+ + + G N+ +F D D
Sbjct: 341 PHNIVHKWTGLADKPSEDMGNFYTAGRDPIFFGHHANVDRMWNIWKTIGGKNRKDFTDTD 400
Query: 887 WLEASFIFYDENRKVVRCKVKDCLITENLGYTYQTERHPW--KDVKKRYKKIVRGRKRSA 1060
WL+A+F+FYDEN+++V+ KV DC+ T L Y YQ PW K+ K + K + K
Sbjct: 401 WLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLPKNTKAKAKTTTKSSKSGV 460
Query: 1061 GEGISL--SPVSEFGSAPRALDTTIRVLVPRPKTTRSKDEKEEASEVLVVDEIKIAHGES 1234
+ L + +S G P+AL++ IRV VPRPK +RSK EKE+ EVL++ I++
Sbjct: 461 AKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRSKKEKEDEEEVLLIKGIELDRENF 520
Query: 1235 IKFDVYIAKPIEGLVGPELGELAGSFVRVAHSHVKKATESRKAKIELGITNLLDDIEAEA 1414
+KFDVYI + P+ E AGSFV V H H+K+ K + I LL+D+ AE
Sbjct: 521 VKFDVYINDEDYSVSRPKNSEFAGSFVNVPHKHMKEM--KTKTNLRFAINELLEDLGAED 578
Query: 1415 SDSLVVSLVPQHG--DVIIGAVHIEY 1486
+S++V++VP+ G DV IG + IE+
Sbjct: 579 DESVIVTIVPRAGGDDVTIGGIEIEF 604
>gb|AAB41022.1| polyphenol oxidase [Vitis vinifera]
Length = 607
Score = 443 bits (1140), Expect = e-122
Identities = 231/506 (45%), Positives = 315/506 (62%), Gaps = 11/506 (2%)
Frame = +2
Query: 2 PVSPPDLSKCQLATDSANGERVNCCPPYTTANVVDF-IPPRKEFLRQRKAAHKLSHDEIR 178
P+ PD+SKC AT NCCPP TT ++DF +P +R R AAH +S + +
Sbjct: 105 PIQAPDISKCGTATVPDGVTPTNCCPPVTT-KIIDFQLPSSGSPMRTRPAAHLVSKEYLA 163
Query: 179 KFQTAIQKLKELPADDPWNYMQQATIHCTYCNGAFDQVGFDDVLLQIHGSWLFTPWHRYY 358
K++ AI+ K LP DDP ++ QQA +HCTYC GA+DQVG+ D+ LQ+H SWLF P+HRYY
Sbjct: 164 KYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQVGYTDLELQVHASWLFLPFHRYY 223
Query: 359 IYFWEKILGKLIGDPTFSIPYWNWDAPDGMYMPQPYLDTSSTLYNGNRNLDHY-SALLDL 535
+YF E+IL KLI DPT ++ YW WD PDGMYMP Y + S+LY+ RN H ++DL
Sbjct: 224 LYFNERILAKLIDDPTIALAYWAWDNPDGMYMPTIYASSPSSLYDEKRNAKHLPPTVIDL 283
Query: 536 DYTFGAPNPTPEQEEEIKIKNLRKVYNMFTETIGSPALFLGTPLRAGDAPD-GAGSLENM 712
DY P ++E+K NL +Y +P LFLG P RAGDA D GAG+LE++
Sbjct: 284 DYDGTEPT---IPDDELKTDNLAIMYKQIVSGATTPKLFLGYPYRAGDAIDPGAGTLEHV 340
Query: 713 -HGTLHQWTGPVAIPRHDMGNFYTAARDTMFFAHHANVDRLWDTYSDLRG-NKVEFNDPD 886
H +H+WTG P DMGNFYTA RD +FF HHANVDR+W+ + + G N+ +F D D
Sbjct: 341 PHNIVHKWTGLADKPSEDMGNFYTAGRDPIFFGHHANVDRMWNIWKTIGGKNRKDFTDTD 400
Query: 887 WLEASFIFYDENRKVVRCKVKDCLITENLGYTYQTERHPW--KDVKKRYKKIVRGRKRSA 1060
WL+A+F+FYDEN+++V+ KV DC+ T L Y YQ PW K K + K + K
Sbjct: 401 WLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLPKKTKAKAKTTTKSSKSGV 460
Query: 1061 GEGISL--SPVSEFGSAPRALDTTIRVLVPRPKTTRSKDEKEEASEVLVVDEIKIAHGES 1234
+ L + +S G P+AL++ IRV VPRPK +RSK EKE+ EVL++ I++
Sbjct: 461 AKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRSKKEKEDEEEVLLIKGIELDRENF 520
Query: 1235 IKFDVYIAKPIEGLVGPELGELAGSFVRVAHSHVKKATESRKAKIELGITNLLDDIEAEA 1414
+KFDVYI + P+ E AGSFV V H H+K+ K + I LL+D+ AE
Sbjct: 521 VKFDVYINDEDYSVSRPKNSEFAGSFVNVPHKHMKEM--KTKTNLRFAINELLEDLGAED 578
Query: 1415 SDSLVVSLVPQHG--DVIIGAVHIEY 1486
+S++V++VP+ G DV IG + IE+
Sbjct: 579 DESVIVTIVPRAGGDDVTIGGIEIEF 604
>gb|AEY78528.1| polyphenol oxidase [Canarium album]
Length = 606
Score = 443 bits (1139), Expect = e-122
Identities = 238/513 (46%), Positives = 320/513 (62%), Gaps = 16/513 (3%)
Frame = +2
Query: 2 PVSPPDLSKCQLATDSANGERVNCCPPYTTANVVDF-IPPRKEFLRQRKAAHKLSHDEIR 178
PVS PDL+KC A A ++ NCCPP T+ ++ F + P LR R AAH + I
Sbjct: 102 PVSAPDLAKCGKANLPAGAKQTNCCPP-TSKKILGFKLHPSNAPLRIRPAAHLVDDAYIA 160
Query: 179 KFQTAIQKLKELPADDPWNYMQQATIHCTYCNGAFDQVGFDDVLLQIHGSWLFTPWHRYY 358
K+ AI+ +K LPADDP +MQQA +HC YC+GA+DQ+GF ++ LQ+H SWLF P+HRYY
Sbjct: 161 KYSRAIELMKALPADDPRGFMQQANVHCAYCDGAYDQIGFPNLELQVHNSWLFFPFHRYY 220
Query: 359 IYFWEKILGKLIGDPTFSIPYWNWDAPDGMYMPQPYLDTSSTLYNGNRNLDHY-SALLDL 535
+YF+EKILGKLI DPTF++P+WNWD+P GM MP Y + S LY+ RN H L+DL
Sbjct: 221 LYFYEKILGKLIEDPTFALPFWNWDSPPGMQMPAMYANPKSPLYDNFRNASHQPPTLVDL 280
Query: 536 DYTFGAPNPTPEQEEEIKIKNLRKVYNMFTETIGSPALFLGTPLRAGDAPD-GAGSLENM 712
DY + T ++++ NL+ +Y + LF G RAGD PD G GS+EN+
Sbjct: 281 DY--NRTDETTTNKDQLS-SNLKIMYRQMVSNGKTAKLFFGNAYRAGDEPDPGLGSIENI 337
Query: 713 -HGTLHQWTGPVAIPR-HDMGNFYTAARDTMFFAHHANVDRLWDTYSDLRGNK-VEFNDP 883
HG +H W G P DMGNFY+AARD +F++HH+N+DR+W + L G K EF DP
Sbjct: 338 PHGPVHLWCGDKTQPNLEDMGNFYSAARDPIFYSHHSNIDRMWTIWKTLGGKKRKEFTDP 397
Query: 884 DWLEASFIFYDENRKVVRCKVKDCLITENLGYTYQTERHPWKDVKKRYKKIVRGRKRSA- 1060
DWL+A F+FYDEN +VR KV+DCL + LGY YQ PW + K +K + + RS
Sbjct: 398 DWLDAGFLFYDENANLVRVKVRDCLDNKKLGYDYQKVDIPWLNTKPTPRKRIVNKVRSTF 457
Query: 1061 -------GEGISLSPVSEFGSAPRALDTTIRVLVPRPKTTRSKDEKEEASEVLVVDEIKI 1219
I L+ S+F P LD T+ LVPRPK +RSK EKEE EVLV++ I
Sbjct: 458 GVAHAAETRRIQLTARSQF---PLVLDKTMSTLVPRPKKSRSKKEKEEEEEVLVIEGIDF 514
Query: 1220 AHGESIKFDVYIAKPIEGLVGPELGELAGSFVRVAHSHVKKATESRKAKIELGITNLLDD 1399
+KFDVYI + GP+ E AGSFV V H H K + K + LG+T+L++D
Sbjct: 515 ERDAQVKFDVYINDEHDVPSGPDDTEFAGSFVNVPHKH--KHGKKIKTCLRLGLTDLIED 572
Query: 1400 IEAEASDSLVVSLVPQHGDVI--IGAVHIEYFE 1492
+EAE DS+VV+LVP++G + IG + IE+++
Sbjct: 573 LEAEDDDSVVVTLVPRYGKGLARIGGIKIEFYD 605