BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00003686
         (1922 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265171.1| PREDICTED: pectinesterase 3-like [Vitis vini...   753   0.0  
ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|2...   717   0.0  
gb|AAB57670.1| pectinesterase [Citrus sinensis]                       716   0.0  
sp|P83948.1|PME3_CITSI RecName: Full=Pectinesterase 3; Short=PE ...   715   0.0  
sp|O04886.1|PME1_CITSI RecName: Full=Pectinesterase 1; Short=PE ...   715   0.0  

>ref|XP_002265171.1| PREDICTED: pectinesterase 3-like [Vitis vinifera]
          Length = 611

 Score =  753 bits (1944), Expect = 0.0
 Identities = 380/579 (65%), Positives = 447/579 (77%), Gaps = 9/579 (1%)
 Frame = +1

Query: 181  FAGMSDSGKFT----RKKKLFFILLSTXXXXXXXXXXXXXXXSRKRSND----TPSHAIL 336
            F GMSDSGK T    R KKLF ++ S+               SRK S +    T  HA+L
Sbjct: 38   FTGMSDSGKHTSISKRNKKLFLLVFSSVLLIAAVVGIVAGVHSRKSSTNDVGLTAGHAVL 97

Query: 337  KSSCSNTRYPELCYSAIASVPGAAAKISTQKDVIEASLNLTLISVKRNYVTVKKILKTRK 516
            KS+CS+TRYP+LCYSAIA+VPGA+ K+++QKDVI  SLN+T+ +V+ NY T++K+L   K
Sbjct: 98   KSACSSTRYPDLCYSAIATVPGASKKVTSQKDVIAVSLNITVTAVEHNYFTIEKLLDF-K 156

Query: 517  SLTKREKTALHDCLEMDDETLDELRKTKNELEEYPKKKSLNQYAEDLKILVSGAMTNQDT 696
            +LTKREK ALHDCLE  DETLDEL    ++L+EYP KKSL Q+A+DLK L+S AMTNQ+T
Sbjct: 157  NLTKREKAALHDCLETIDETLDELHVAMDDLDEYPDKKSLTQHADDLKTLMSAAMTNQET 216

Query: 697  CIDGFSHSKADKEVRKLLFKGEMHVFHMLSNALAMICNLTNTDMLNQHPISGRNLVEENV 876
            C+DGFSH  ADK VR++L KG+ HV HM SNALAMI N+T+TD+  +     R L+EE  
Sbjct: 217  CLDGFSHDDADKHVREVLLKGQRHVEHMCSNALAMIKNMTDTDIAREREAMNRKLMEER- 275

Query: 877  NKIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXXDGSGNYRTXXXXXXXXPEGSSSRYIIR 1056
               D  G+PKWLSAGDRRLL             DGSG+Y+T        PE SS RYII 
Sbjct: 276  ---DESGWPKWLSAGDRRLLQSSSVTPDVVVAADGSGDYKTVSAAVAAAPEKSSKRYIIG 332

Query: 1057 IKAGTYRENVEVPRKKSNIMFMGDGRRNTIITASRNVVDGSTTFNSYTVAVVGERFLARD 1236
            IKAG Y+ENVEV +KK+NIMF+GDGR NTIIT S+NVVDGSTTFNS TVAVVGE+F+ARD
Sbjct: 333  IKAGVYKENVEVGKKKTNIMFLGDGRSNTIITGSKNVVDGSTTFNSATVAVVGEKFIARD 392

Query: 1237 ITFENTAGPSKGQAVALRVGADLSAFYQCDMLAYQDTLYVHSLRQFYVKCLVAGTVDFIF 1416
            ITF+NTAGPSK QAVALRVG+DLSAFY+CDMLAYQDTLYVHS RQFY+ CLVAGTVDFIF
Sbjct: 393  ITFQNTAGPSKHQAVALRVGSDLSAFYKCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIF 452

Query: 1417 GNAAVVFQDCDLHARRPNRGQANMFTAQGRDDPNQNTGIVIQKCRIGATSDLLPVIGSIR 1596
            GNAA VFQDCD+HARRPN GQ NM TAQGR DPNQNTGIVIQKCRIGATSDL  VI S +
Sbjct: 453  GNAAAVFQDCDIHARRPNSGQKNMLTAQGRTDPNQNTGIVIQKCRIGATSDLQAVISSFK 512

Query: 1597 SYLGRPWRRYSRTVVMQNTISNVIDPLGWNPWTGEF-LNTLYYAEYQNTGAGAGTGNRVN 1773
            +YLGRPW+ YSRTVVMQ +I+NVIDP GW+ W+G F L+TLYY EYQNTGAGAGT  RV 
Sbjct: 513  TYLGRPWKEYSRTVVMQTSITNVIDPAGWHEWSGSFALSTLYYGEYQNTGAGAGTSKRVT 572

Query: 1774 WSGFRVLTNAADVQPFTVGRFLQGSSWLGSTGFPFSLGL 1890
            W GF+V+T+A++ Q FT G F+ GSSWLGSTGFP+SLGL
Sbjct: 573  WKGFKVITSASEAQGFTPGTFIAGSSWLGSTGFPYSLGL 611


>ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|222841689|gb|EEE79236.1|
            predicted protein [Populus trichocarpa]
          Length = 579

 Score =  717 bits (1852), Expect = 0.0
 Identities = 365/583 (62%), Positives = 442/583 (75%), Gaps = 5/583 (0%)
 Frame = +1

Query: 157  MTHIKDNVFAGMSDSGK----FTRKKKLFFILLSTXXXXXXXXXXXXXXXSRKRSNDTPS 324
            MT  K ++  G+SDSGK    F + KKLF ++L++               S K S +  +
Sbjct: 1    MTQDKQSL-TGISDSGKHISIFKKNKKLFLVILASLLLVTAIIAIVAGVNSHKSSKNEGT 59

Query: 325  HAILKSSCSNTRYPELCYSAIASVPGAAAKISTQKDVIEASLNLTLISVKRNYVTVKKIL 504
            HAILKSSCS+T YPELCYSA+A+VPGA + +++QKDVIE S+NLT  +V+ N+ TV+K++
Sbjct: 60   HAILKSSCSSTLYPELCYSAVATVPGATSNLASQKDVIELSINLTTKAVQHNFFTVEKLI 119

Query: 505  KTRKSLTKREKTALHDCLEMDDETLDELRKTKNELEEYPKKKSLNQYAEDLKILVSGAMT 684
             T+K LTKREKTALHDCLE+ DETLDEL +   +L +YP  KSL ++A+DLK L+S A+T
Sbjct: 120  ATKK-LTKREKTALHDCLEIIDETLDELHEALVDLNDYPNNKSLKKHADDLKTLLSSAIT 178

Query: 685  NQDTCIDGFSHSKADKEVRKLLFKGEMHVFHMLSNALAMICNLTNTDMLNQHPISGRNLV 864
            NQ+TC+DGFSH +ADK+VRK L KG++HV  M SN LAMI N+T+TD+ N+   + R L+
Sbjct: 179  NQETCLDGFSHDEADKKVRKALLKGQIHVEKMCSNVLAMIKNMTDTDVANELKTTNRKLM 238

Query: 865  EENVNKIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXXDGSGNYRTXXXXXXXXPEGSSSR 1044
            +E     +   +P+W+S  DRRLL             DGSGNY+T        P+ SS R
Sbjct: 239  QEKEG--NESEWPEWMSVADRRLLQSSSVTPDVVVAADGSGNYKTVSAAVAAAPKKSSKR 296

Query: 1045 YIIRIKAGTYRENVEVPRKKSNIMFMGDGRRNTIITASRNVVDGSTTFNSYTVAVVGERF 1224
            YIIRIKAG YRENV+VP+ K+NIMFMGDGR+ TIITASRNVVDGSTTFNS TVA VG+ F
Sbjct: 297  YIIRIKAGVYRENVDVPKDKTNIMFMGDGRKTTIITASRNVVDGSTTFNSATVAAVGQGF 356

Query: 1225 LARDITFENTAGPSKGQAVALRVGADLSAFYQCDMLAYQDTLYVHSLRQFYVKCLVAGTV 1404
            LAR ITF+NTAGPSK QAVALRVG+DLSAFY CDMLAYQDTLYVHS RQF++ CLVAGTV
Sbjct: 357  LARGITFQNTAGPSKHQAVALRVGSDLSAFYDCDMLAYQDTLYVHSNRQFFINCLVAGTV 416

Query: 1405 DFIFGNAAVVFQDCDLHARRPNRGQANMFTAQGRDDPNQNTGIVIQKCRIGATSDLLPVI 1584
            DFIFGNAAVV QDCD+HARRPN GQ NM TAQGR DPNQNTGIVIQK RIGATSDL PV 
Sbjct: 417  DFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPVK 476

Query: 1585 GSIRSYLGRPWRRYSRTVVMQNTISNVIDPLGWNPWTGEF-LNTLYYAEYQNTGAGAGTG 1761
             S  +YLGRPW+ YSRTV+MQ++I++VI P GW  W+G F LNTLYYAEYQN+GAGAGT 
Sbjct: 477  SSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWFEWSGSFALNTLYYAEYQNSGAGAGTS 536

Query: 1762 NRVNWSGFRVLTNAADVQPFTVGRFLQGSSWLGSTGFPFSLGL 1890
             RV W G+RV+T+A + Q FT G F+ GSSWL ST FPFSLGL
Sbjct: 537  RRVTWKGYRVITSATEAQRFTPGNFIAGSSWLRSTTFPFSLGL 579


>gb|AAB57670.1| pectinesterase [Citrus sinensis]
          Length = 584

 Score =  716 bits (1849), Expect = 0.0
 Identities = 372/589 (63%), Positives = 434/589 (73%), Gaps = 11/589 (1%)
 Frame = +1

Query: 157  MTHIKDNVFAGMSDSGKFT-------RKKKLFFILLSTXXXXXXXXXXXXXXXSRKRSND 315
            MTHIK+  F  +S+S           +KKKLF  L +T               SRK S D
Sbjct: 1    MTHIKE-FFTKLSESSSNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD 59

Query: 316  T---PSHAILKSSCSNTRYPELCYSAIASVPGAAAKISTQKDVIEASLNLTLISVKRNYV 486
                P HAILKSSCS+TRYP+LC+SAIA+VP A+ K+++QKDVIE SLN+T  +V+ NY 
Sbjct: 60   NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 487  TVKKILKTRKSLTKREKTALHDCLEMDDETLDELRKTKNELEEYPKKKSLNQYAEDLKIL 666
             ++K+LK R +LTKREK ALHDCLE  DETLDEL K   +LEEYP KKSL+Q+A+DLK L
Sbjct: 120  GIQKLLK-RTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTL 178

Query: 667  VSGAMTNQDTCIDGFSHSKADKEVRKLLFKGEMHVFHMLSNALAMICNLTNTDMLNQHPI 846
            +S AMTNQ TC+DGFSH  A+K VR  L  G++HV  M SNALAMI N+T+TDM+     
Sbjct: 179  MSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTS 238

Query: 847  SGRNLVEENVNKIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXXDGSGNYRTXXXXXXXXP 1026
            + R L EE       DG+P WLS GDRRLL             DGSGN++T        P
Sbjct: 239  NNRKLTEETSTV---DGWPAWLSPGDRRLLQSSSVTPNAVVAADGSGNFKTVAAAVAAAP 295

Query: 1027 EGSSSRYIIRIKAGTYRENVEVPRKKSNIMFMGDGRRNTIITASRNVVDGSTTFNSYTVA 1206
            +G + RYIIRIKAG YRENVEV +K  NIMF+GDGR  TIIT SRNVVDGSTTF S TVA
Sbjct: 296  QGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 355

Query: 1207 VVGERFLARDITFENTAGPSKGQAVALRVGADLSAFYQCDMLAYQDTLYVHSLRQFYVKC 1386
            VVGE FLARDITF+NTAGPSK QAVALRVGADLSAFY CDMLAYQDTLYVHS RQF+V C
Sbjct: 356  VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 415

Query: 1387 LVAGTVDFIFGNAAVVFQDCDLHARRPNRGQANMFTAQGRDDPNQNTGIVIQKCRIGATS 1566
            L+AGTVDFIFGNAA V Q+CD+HAR+PN GQ NM TAQGR DPNQNTGIVIQK RIGATS
Sbjct: 416  LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATS 475

Query: 1567 DLLPVIGSIRSYLGRPWRRYSRTVVMQNTISNVIDPLGWNPWTGEF-LNTLYYAEYQNTG 1743
            DL PV GS  +YLGRPW+ YSRTV+MQ++I+++I P GW+ W G F LNTL+Y E+QN+G
Sbjct: 476  DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSG 535

Query: 1744 AGAGTGNRVNWSGFRVLTNAADVQPFTVGRFLQGSSWLGSTGFPFSLGL 1890
            AGAGT  RV W GFRV+T+A + Q FT G F+ GSSWLGSTGFPFSLGL
Sbjct: 536  AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584


>sp|P83948.1|PME3_CITSI RecName: Full=Pectinesterase 3; Short=PE 3; AltName: Full=Pectin
            methylesterase 3; Flags: Precursor
          Length = 584

 Score =  715 bits (1845), Expect = 0.0
 Identities = 372/589 (63%), Positives = 433/589 (73%), Gaps = 11/589 (1%)
 Frame = +1

Query: 157  MTHIKDNVFAGMSDSGKFT-------RKKKLFFILLSTXXXXXXXXXXXXXXXSRKRSND 315
            MT IK+  F  +S+S           +KKKLF  L +T               SRK S D
Sbjct: 1    MTRIKE-FFTKLSESSTNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD 59

Query: 316  T---PSHAILKSSCSNTRYPELCYSAIASVPGAAAKISTQKDVIEASLNLTLISVKRNYV 486
                P HAILKSSCS+TRYP+LC+SAIA+VP A+ K+++QKDVIE SLN+T  +V+ NY 
Sbjct: 60   NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 487  TVKKILKTRKSLTKREKTALHDCLEMDDETLDELRKTKNELEEYPKKKSLNQYAEDLKIL 666
             ++K+LK R +LTKREK ALHDCLE  DETLDEL K   +LEEYP KKSL+Q+A+DLK L
Sbjct: 120  GIQKLLK-RTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTL 178

Query: 667  VSGAMTNQDTCIDGFSHSKADKEVRKLLFKGEMHVFHMLSNALAMICNLTNTDMLNQHPI 846
            +S AMTNQ TC+DGFSH  A+K VR  L  G++HV  M SNALAMI N+T+TDM+     
Sbjct: 179  MSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTS 238

Query: 847  SGRNLVEENVNKIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXXDGSGNYRTXXXXXXXXP 1026
            + R L+EE       DG+P WLS GDRRLL             DGSGN++T        P
Sbjct: 239  NNRKLIEETSTV---DGWPAWLSTGDRRLLQSSSVTPNVVVAADGSGNFKTVAASVAAAP 295

Query: 1027 EGSSSRYIIRIKAGTYRENVEVPRKKSNIMFMGDGRRNTIITASRNVVDGSTTFNSYTVA 1206
            +G + RYIIRIKAG YRENVEV +K  NIMF+GDGR  TIIT SRNVVDGSTTF S TVA
Sbjct: 296  QGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 355

Query: 1207 VVGERFLARDITFENTAGPSKGQAVALRVGADLSAFYQCDMLAYQDTLYVHSLRQFYVKC 1386
            VVGE FLARDITF+NTAGPSK QAVALRVGADLSAFY CDMLAYQDTLYVHS RQF+V C
Sbjct: 356  VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 415

Query: 1387 LVAGTVDFIFGNAAVVFQDCDLHARRPNRGQANMFTAQGRDDPNQNTGIVIQKCRIGATS 1566
            L+AGTVDFIFGNAA V Q+CD+HAR+PN GQ NM TAQGR DPNQNTGIVIQK RIGATS
Sbjct: 416  LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATS 475

Query: 1567 DLLPVIGSIRSYLGRPWRRYSRTVVMQNTISNVIDPLGWNPWTGEF-LNTLYYAEYQNTG 1743
            DL PV GS  +YLGRPW+ YSRTV+MQ++I++VI P GW+ W G F LNTL+Y E+QN G
Sbjct: 476  DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAG 535

Query: 1744 AGAGTGNRVNWSGFRVLTNAADVQPFTVGRFLQGSSWLGSTGFPFSLGL 1890
            AGAGT  RV W GFRV+T+A + Q FT G F+ GSSWLGSTGFPFSLGL
Sbjct: 536  AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584


>sp|O04886.1|PME1_CITSI RecName: Full=Pectinesterase 1; Short=PE 1; AltName: Full=Pectin
            methylesterase; Flags: Precursor
            gi|2098705|gb|AAB57667.1| pectinesterase [Citrus
            sinensis]
          Length = 584

 Score =  715 bits (1845), Expect = 0.0
 Identities = 371/589 (62%), Positives = 433/589 (73%), Gaps = 11/589 (1%)
 Frame = +1

Query: 157  MTHIKDNVFAGMSDSGKFT-------RKKKLFFILLSTXXXXXXXXXXXXXXXSRKRSND 315
            MTHIK+  F  +S+S           +KKKLF  L +T               SRK S D
Sbjct: 1    MTHIKE-FFTKLSESSSNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD 59

Query: 316  T---PSHAILKSSCSNTRYPELCYSAIASVPGAAAKISTQKDVIEASLNLTLISVKRNYV 486
                P HAILKSSCS+TRYP+LC+SAIA+VP A+ K+++QKDVIE SLN+T  +V+ NY 
Sbjct: 60   NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 487  TVKKILKTRKSLTKREKTALHDCLEMDDETLDELRKTKNELEEYPKKKSLNQYAEDLKIL 666
             ++K+LK R +LTKREK ALHDCLE  DETLDEL K   +LEEYP KKSL+Q+A+DLK L
Sbjct: 120  GIQKLLK-RTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTL 178

Query: 667  VSGAMTNQDTCIDGFSHSKADKEVRKLLFKGEMHVFHMLSNALAMICNLTNTDMLNQHPI 846
            +S AMTNQ TC+DGFSH  A+K VR  L  G++HV  M SNALAMI N+T+TDM+     
Sbjct: 179  MSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTS 238

Query: 847  SGRNLVEENVNKIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXXDGSGNYRTXXXXXXXXP 1026
            + R L EE       DG+P WLS GDRRLL             DGSGN++T        P
Sbjct: 239  NNRKLTEETSTV---DGWPAWLSPGDRRLLQSSSVTPNAVVAADGSGNFKTVAAAVAAAP 295

Query: 1027 EGSSSRYIIRIKAGTYRENVEVPRKKSNIMFMGDGRRNTIITASRNVVDGSTTFNSYTVA 1206
            +G + RYIIRIKAG YRENVEV +K  NIMF+GDGR  TIIT SRNVVDGSTTF S T A
Sbjct: 296  QGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAA 355

Query: 1207 VVGERFLARDITFENTAGPSKGQAVALRVGADLSAFYQCDMLAYQDTLYVHSLRQFYVKC 1386
            VVGE FLARDITF+NTAGPSK QAVALRVGADLSAFY CDMLAYQDTLYVHS RQF+V C
Sbjct: 356  VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 415

Query: 1387 LVAGTVDFIFGNAAVVFQDCDLHARRPNRGQANMFTAQGRDDPNQNTGIVIQKCRIGATS 1566
            L+AGTVDFIFGNAA V Q+CD+HAR+PN GQ NM TAQGR DPNQNTGIVIQK RIGATS
Sbjct: 416  LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATS 475

Query: 1567 DLLPVIGSIRSYLGRPWRRYSRTVVMQNTISNVIDPLGWNPWTGEF-LNTLYYAEYQNTG 1743
            DL PV GS  +YLGRPW+ YSRTV+MQ++I+++I P GW+ W G F LNTL+Y E+QN+G
Sbjct: 476  DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSG 535

Query: 1744 AGAGTGNRVNWSGFRVLTNAADVQPFTVGRFLQGSSWLGSTGFPFSLGL 1890
            AGAGT  RV W GFRV+T+A + Q FT G F+ GSSWLGSTGFPFSLGL
Sbjct: 536  AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584