BLASTX nr result
ID: Coptis23_contig00003685
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00003685
(1987 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002265171.1| PREDICTED: pectinesterase 3-like [Vitis vini... 723 0.0
gb|AAC72288.1| putative pectin methylesterase [Arabidopsis thali... 705 0.0
ref|NP_188048.1| pectinesterase 3 [Arabidopsis thaliana] gi|2298... 703 0.0
ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|2... 703 0.0
gb|AAF23892.1|AF152172_1 pectin methyl esterase [Solanum tuberosum] 702 0.0
>ref|XP_002265171.1| PREDICTED: pectinesterase 3-like [Vitis vinifera]
Length = 611
Score = 723 bits (1865), Expect = 0.0
Identities = 362/528 (68%), Positives = 423/528 (80%)
Frame = -2
Query: 1707 IASVPGVAAKISTQKDVIEASLNLTITAVEHNYFTIKKIIKTRKALTKREKTALHDCLEM 1528
IA+VPG + K+++QKDVI SLN+T+TAVEHNYFTI+K++ K LTKREK ALHDCLE
Sbjct: 114 IATVPGASKKVTSQKDVIAVSLNITVTAVEHNYFTIEKLLDF-KNLTKREKAALHDCLET 172
Query: 1527 IDETLDEIRDTEDELDDYPKKKSLNQYAEDLKTLVSGAMTNQDSCLDGFSYEKADKEVRK 1348
IDETLDE+ D+LD+YP KKSL Q+A+DLKTL+S AMTNQ++CLDGFS++ ADK VR+
Sbjct: 173 IDETLDELHVAMDDLDEYPDKKSLTQHADDLKTLMSAAMTNQETCLDGFSHDDADKHVRE 232
Query: 1347 LLIAGQVHVYHMLSNALAMICNMTNTDMFNQRSVSGRNLVEEEDVNKIDSDGFPKWLSAG 1168
+L+ GQ HV HM SNALAMI NMT+TD+ +R R L+EE D G+PKWLSAG
Sbjct: 233 VLLKGQRHVEHMCSNALAMIKNMTDTDIAREREAMNRKLMEERD-----ESGWPKWLSAG 287
Query: 1167 DRRLLXXXXXXXXXXXXADGSGNYRTXXXXXXXAPEKSSTRYIIRIKAGTYRENVEIPKK 988
DRRLL ADGSG+Y+T APEKSS RYII IKAG Y+ENVE+ KK
Sbjct: 288 DRRLLQSSSVTPDVVVAADGSGDYKTVSAAVAAAPEKSSKRYIIGIKAGVYKENVEVGKK 347
Query: 987 KTNIMFMGDGRRNTIITGSRNVVDGSTTFNSATVAAVGERFLARDITFENTAGPSKHQAV 808
KTNIMF+GDGR NTIITGS+NVVDGSTTFNSATVA VGE+F+ARDITF+NTAGPSKHQAV
Sbjct: 348 KTNIMFLGDGRSNTIITGSKNVVDGSTTFNSATVAVVGEKFIARDITFQNTAGPSKHQAV 407
Query: 807 ALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKCIVAGTVDFIFGNAAAVLQDCDLHAR 628
ALRVGSDLSAFY+CDMLAYQDTLYVHS RQFY+ C+VAGTVDFIFGNAAAV QDCD+HAR
Sbjct: 408 ALRVGSDLSAFYKCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIFGNAAAVFQDCDIHAR 467
Query: 627 RPNSGQRNMFTAQGRDDPNQNTGIVIQKCRIGATSDLLPVIGSFPSFLGRPWKEYSRTIV 448
RPNSGQ+NM TAQGR DPNQNTGIVIQKCRIGATSDL VI SF ++LGRPWKEYSRT+V
Sbjct: 468 RPNSGQKNMLTAQGRTDPNQNTGIVIQKCRIGATSDLQAVISSFKTYLGRPWKEYSRTVV 527
Query: 447 MQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTGAGAGTANRVKWRGYRVLTNAADVQP 268
MQ+SI+NVI+PAGW+EW+G+FAL+TLYY EYQNTGAGAGT+ RV W+G++V+T+A++ Q
Sbjct: 528 MQTSITNVIDPAGWHEWSGSFALSTLYYGEYQNTGAGAGTSKRVTWKGFKVITSASEAQG 587
Query: 267 FTVGRFIQGSSWLGXXXXXXXXXXXXXXXXXQGSSWLGSTGFPFSLGL 124
FT G FI G SSWLGSTGFP+SLGL
Sbjct: 588 FTPGTFIAG------------------------SSWLGSTGFPYSLGL 611
>gb|AAC72288.1| putative pectin methylesterase [Arabidopsis thaliana]
Length = 592
Score = 705 bits (1819), Expect = 0.0
Identities = 359/542 (66%), Positives = 416/542 (76%), Gaps = 15/542 (2%)
Frame = -2
Query: 1704 ASVPGVAAKISTQKDVIEASLNLTITAVEHNYFTIKKIIKTRKALTKREKTALHDCLEMI 1525
A V A ++++QKDVIEAS+NLTITAVEHNYFT+KK+IK RK LT REKTALHDCLE I
Sbjct: 75 AVVTAGACELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 134
Query: 1524 DETLDEIRDTEDELDDYPKKKSLNQYAEDLKTLVSGAMTNQDSCLDGFSYEKADKEVRKL 1345
DETLDE+ +T ++L YP KK+L ++A DLKTL+S A+TNQ++CLDGFS++ ADK+VRK
Sbjct: 135 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 194
Query: 1344 LIAGQVHVYHMLSNALAMICNMTNTDMFN-----QRSVSGRNLVEEED----------VN 1210
L+ GQ+HV HM SNALAMI NMT+TD+ N + + + R L EE
Sbjct: 195 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAG 254
Query: 1209 KIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXADGSGNYRTXXXXXXXAPEKSSTRYIIRI 1030
++DS+G+P WLSAGDRRLL ADGSG ++T APE S+ RY+I I
Sbjct: 255 ELDSEGWPTWLSAGDRRLLQGSGVKRDATVAADGSGTFKTVAAAVAAAPENSNKRYVIHI 314
Query: 1029 KAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFNSATVAAVGERFLARDI 850
KAG YRENVE+ KKK NIMFMGDGR TIITGSRNVVDGSTTF+SATVAAVGERFLARDI
Sbjct: 315 KAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDI 374
Query: 849 TFENTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKCIVAGTVDFIFG 670
TF+NTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLYVHS RQF+VKC++AGTVDFIFG
Sbjct: 375 TFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFG 434
Query: 669 NAAAVLQDCDLHARRPNSGQRNMFTAQGRDDPNQNTGIVIQKCRIGATSDLLPVIGSFPS 490
NAA VLQDCD+HARRPNSGQ+NM TAQGR DPNQNTGIVIQKCRIGATSDL V GSFP+
Sbjct: 435 NAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPT 494
Query: 489 FLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTGAGAGTANRVKW 310
+LGRPWKEYS+T++MQS+IS+VI P GW EW G FALNTL Y EY NTGAGAGTANRVKW
Sbjct: 495 YLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKW 554
Query: 309 RGYRVLTNAADVQPFTVGRFIQGSSWLGXXXXXXXXXXXXXXXXXQGSSWLGSTGFPFSL 130
RG++V+T AA+ Q +T G+FI G WL STGFPFSL
Sbjct: 555 RGFKVITAAAEAQKYTAGQFI------------------------GGGGWLSSTGFPFSL 590
Query: 129 GL 124
GL
Sbjct: 591 GL 592
>ref|NP_188048.1| pectinesterase 3 [Arabidopsis thaliana]
gi|229891485|sp|O49006.2|PME3_ARATH RecName:
Full=Pectinesterase/pectinesterase inhibitor 3; Includes:
RecName: Full=Pectinesterase inhibitor 3; AltName:
Full=Pectin methylesterase inhibitor 3; Includes:
RecName: Full=Pectinesterase 3; Short=PE 3; AltName:
Full=Pectin methylesterase 27; Short=AtPME27; AltName:
Full=Pectin methylesterase 3; Flags: Precursor
gi|9279579|dbj|BAB01037.1| pectinesterase [Arabidopsis
thaliana] gi|14335010|gb|AAK59769.1| AT3g14310/MLN21_9
[Arabidopsis thaliana] gi|15529256|gb|AAK97722.1|
AT3g14310/MLN21_9 [Arabidopsis thaliana]
gi|23506059|gb|AAN28889.1| At3g14310/MLN21_9 [Arabidopsis
thaliana] gi|332641979|gb|AEE75500.1| pectinesterase 3
[Arabidopsis thaliana]
Length = 592
Score = 703 bits (1815), Expect = 0.0
Identities = 358/542 (66%), Positives = 415/542 (76%), Gaps = 15/542 (2%)
Frame = -2
Query: 1704 ASVPGVAAKISTQKDVIEASLNLTITAVEHNYFTIKKIIKTRKALTKREKTALHDCLEMI 1525
A V ++++QKDVIEAS+NLTITAVEHNYFT+KK+IK RK LT REKTALHDCLE I
Sbjct: 75 AVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 134
Query: 1524 DETLDEIRDTEDELDDYPKKKSLNQYAEDLKTLVSGAMTNQDSCLDGFSYEKADKEVRKL 1345
DETLDE+ +T ++L YP KK+L ++A DLKTL+S A+TNQ++CLDGFS++ ADK+VRK
Sbjct: 135 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 194
Query: 1344 LIAGQVHVYHMLSNALAMICNMTNTDMFN-----QRSVSGRNLVEEED----------VN 1210
L+ GQ+HV HM SNALAMI NMT+TD+ N + + + R L EE
Sbjct: 195 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAG 254
Query: 1209 KIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXADGSGNYRTXXXXXXXAPEKSSTRYIIRI 1030
++DS+G+P WLSAGDRRLL ADGSG ++T APE S+ RY+I I
Sbjct: 255 ELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVIHI 314
Query: 1029 KAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFNSATVAAVGERFLARDI 850
KAG YRENVE+ KKK NIMFMGDGR TIITGSRNVVDGSTTF+SATVAAVGERFLARDI
Sbjct: 315 KAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDI 374
Query: 849 TFENTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKCIVAGTVDFIFG 670
TF+NTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLYVHS RQF+VKC++AGTVDFIFG
Sbjct: 375 TFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFG 434
Query: 669 NAAAVLQDCDLHARRPNSGQRNMFTAQGRDDPNQNTGIVIQKCRIGATSDLLPVIGSFPS 490
NAA VLQDCD+HARRPNSGQ+NM TAQGR DPNQNTGIVIQKCRIGATSDL V GSFP+
Sbjct: 435 NAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPT 494
Query: 489 FLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTGAGAGTANRVKW 310
+LGRPWKEYS+T++MQS+IS+VI P GW EW G FALNTL Y EY NTGAGAGTANRVKW
Sbjct: 495 YLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKW 554
Query: 309 RGYRVLTNAADVQPFTVGRFIQGSSWLGXXXXXXXXXXXXXXXXXQGSSWLGSTGFPFSL 130
RG++V+T AA+ Q +T G+FI G WL STGFPFSL
Sbjct: 555 RGFKVITAAAEAQKYTAGQFI------------------------GGGGWLSSTGFPFSL 590
Query: 129 GL 124
GL
Sbjct: 591 GL 592
>ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|222841689|gb|EEE79236.1|
predicted protein [Populus trichocarpa]
Length = 579
Score = 703 bits (1814), Expect = 0.0
Identities = 350/528 (66%), Positives = 421/528 (79%)
Frame = -2
Query: 1707 IASVPGVAAKISTQKDVIEASLNLTITAVEHNYFTIKKIIKTRKALTKREKTALHDCLEM 1528
+A+VPG + +++QKDVIE S+NLT AV+HN+FT++K+I T+K LTKREKTALHDCLE+
Sbjct: 80 VATVPGATSNLASQKDVIELSINLTTKAVQHNFFTVEKLIATKK-LTKREKTALHDCLEI 138
Query: 1527 IDETLDEIRDTEDELDDYPKKKSLNQYAEDLKTLVSGAMTNQDSCLDGFSYEKADKEVRK 1348
IDETLDE+ + +L+DYP KSL ++A+DLKTL+S A+TNQ++CLDGFS+++ADK+VRK
Sbjct: 139 IDETLDELHEALVDLNDYPNNKSLKKHADDLKTLLSSAITNQETCLDGFSHDEADKKVRK 198
Query: 1347 LLIAGQVHVYHMLSNALAMICNMTNTDMFNQRSVSGRNLVEEEDVNKIDSDGFPKWLSAG 1168
L+ GQ+HV M SN LAMI NMT+TD+ N+ + R L++E++ N+ + +P+W+S
Sbjct: 199 ALLKGQIHVEKMCSNVLAMIKNMTDTDVANELKTTNRKLMQEKEGNESE---WPEWMSVA 255
Query: 1167 DRRLLXXXXXXXXXXXXADGSGNYRTXXXXXXXAPEKSSTRYIIRIKAGTYRENVEIPKK 988
DRRLL ADGSGNY+T AP+KSS RYIIRIKAG YRENV++PK
Sbjct: 256 DRRLLQSSSVTPDVVVAADGSGNYKTVSAAVAAAPKKSSKRYIIRIKAGVYRENVDVPKD 315
Query: 987 KTNIMFMGDGRRNTIITGSRNVVDGSTTFNSATVAAVGERFLARDITFENTAGPSKHQAV 808
KTNIMFMGDGR+ TIIT SRNVVDGSTTFNSATVAAVG+ FLAR ITF+NTAGPSKHQAV
Sbjct: 316 KTNIMFMGDGRKTTIITASRNVVDGSTTFNSATVAAVGQGFLARGITFQNTAGPSKHQAV 375
Query: 807 ALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKCIVAGTVDFIFGNAAAVLQDCDLHAR 628
ALRVGSDLSAFY CDMLAYQDTLYVHS RQF++ C+VAGTVDFIFGNAA VLQDCD+HAR
Sbjct: 376 ALRVGSDLSAFYDCDMLAYQDTLYVHSNRQFFINCLVAGTVDFIFGNAAVVLQDCDIHAR 435
Query: 627 RPNSGQRNMFTAQGRDDPNQNTGIVIQKCRIGATSDLLPVIGSFPSFLGRPWKEYSRTIV 448
RPNSGQ+NM TAQGR DPNQNTGIVIQK RIGATSDL PV SFP++LGRPWKEYSRT++
Sbjct: 436 RPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPVKSSFPTYLGRPWKEYSRTVI 495
Query: 447 MQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTGAGAGTANRVKWRGYRVLTNAADVQP 268
MQSSI++VI PAGW+EW+G+FALNTLYYAEYQN+GAGAGT+ RV W+GYRV+T+A + Q
Sbjct: 496 MQSSITDVIQPAGWFEWSGSFALNTLYYAEYQNSGAGAGTSRRVTWKGYRVITSATEAQR 555
Query: 267 FTVGRFIQGSSWLGXXXXXXXXXXXXXXXXXQGSSWLGSTGFPFSLGL 124
FT G FI GSSWL ST FPFSLGL
Sbjct: 556 FTPGNFI------------------------AGSSWLRSTTFPFSLGL 579
>gb|AAF23892.1|AF152172_1 pectin methyl esterase [Solanum tuberosum]
Length = 576
Score = 702 bits (1813), Expect = 0.0
Identities = 352/529 (66%), Positives = 417/529 (78%), Gaps = 1/529 (0%)
Frame = -2
Query: 1707 IASVPGVAAKISTQKDVIEASLNLTITAVEHNYFTIKKIIKTRKALTKREKTALHDCLEM 1528
I +V + K+++QKDVIE SLN+T+ AV NY+ +K++IKTRK LT REK ALHDCLE
Sbjct: 79 IVNVTDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVALHDCLET 138
Query: 1527 IDETLDEIRDTEDELDDYPKKKSLNQYAEDLKTLVSGAMTNQDSCLDGFSYEKADKEVRK 1348
+DETLDE+ +L+ YP KKSL ++AEDLKTL+S A+TNQ++CLDGFS+++ADK+VRK
Sbjct: 139 MDETLDELHTAVADLELYPNKKSLKEHAEDLKTLISSAITNQETCLDGFSHDEADKKVRK 198
Query: 1347 LLIAGQVHVYHMLSNALAMICNMTNTDMFNQRSVSG-RNLVEEEDVNKIDSDGFPKWLSA 1171
+L+ GQ HV M SNALAMICNMTNTD+ N+ +SG R LVE D+ +P+WLSA
Sbjct: 199 VLLKGQKHVEKMCSNALAMICNMTNTDIANEMKLSGSRKLVE-------DNGEWPEWLSA 251
Query: 1170 GDRRLLXXXXXXXXXXXXADGSGNYRTXXXXXXXAPEKSSTRYIIRIKAGTYRENVEIPK 991
GDRRLL ADGSG+Y+T APEKSS RY+IRIKAG YRENV++PK
Sbjct: 252 GDRRLLQSSTVTPDVVVAADGSGDYKTVSEAVAKAPEKSSKRYVIRIKAGVYRENVDVPK 311
Query: 990 KKTNIMFMGDGRRNTIITGSRNVVDGSTTFNSATVAAVGERFLARDITFENTAGPSKHQA 811
KKTNIMFMGDGR NTIIT SRNV DGSTTF+SATVAAVGE+FLARDITF+NTAG SKHQA
Sbjct: 312 KKTNIMFMGDGRSNTIITASRNVQDGSTTFHSATVAAVGEKFLARDITFQNTAGASKHQA 371
Query: 810 VALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKCIVAGTVDFIFGNAAAVLQDCDLHA 631
VALRVGSDLSAFY+CD+LAYQDTLYVHS RQF+V+C+VAGTVDFIFGN AAVLQDCD+HA
Sbjct: 372 VALRVGSDLSAFYKCDILAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVLQDCDIHA 431
Query: 630 RRPNSGQRNMFTAQGRDDPNQNTGIVIQKCRIGATSDLLPVIGSFPSFLGRPWKEYSRTI 451
RRP SGQ+NM TAQGR DPNQNTGIVIQKCRIGATSDL PV SFP++LGRPWKEYSRT+
Sbjct: 432 RRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTV 491
Query: 450 VMQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTGAGAGTANRVKWRGYRVLTNAADVQ 271
+MQSSI++VI PAGW+EWNGNFALNTL+Y EY NTGAGA T+ RVKW+G++V+T++ + Q
Sbjct: 492 IMQSSITDVIQPAGWHEWNGNFALNTLFYGEYANTGAGAATSGRVKWKGHKVITSSTEAQ 551
Query: 270 PFTVGRFIQGSSWLGXXXXXXXXXXXXXXXXXQGSSWLGSTGFPFSLGL 124
+T G FI G SWL STGFPFSLGL
Sbjct: 552 AYTPGSFI------------------------AGGSWLSSTGFPFSLGL 576