BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00003499
         (1677 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI27690.3| unnamed protein product [Vitis vinifera]              477   e-171
ref|XP_002330437.1| predicted protein [Populus trichocarpa] gi|2...   479   e-171
ref|XP_002268418.1| PREDICTED: E3 ubiquitin-protein ligase CHFR-...   475   e-171
ref|XP_004146401.1| PREDICTED: E3 ubiquitin-protein ligase CHFR-...   426   e-153
ref|XP_003630425.1| E3 ubiquitin-protein ligase CHFR [Medicago t...   425   e-152

>emb|CBI27690.3| unnamed protein product [Vitis vinifera]
          Length = 1150

 Score =  477 bits (1228), Expect(2) = e-171
 Identities = 229/379 (60%), Positives = 287/379 (75%), Gaps = 3/379 (0%)
 Frame = +3

Query: 84   VKLEPGESSDKPVTEP--WAKLVRLDSKQVDIEIRVDEMVICSENKLLSIGERGWCKITR 257
            +K+E GESS    ++   WAKLV  DS+  ++E+R+DEMVICS+    S  ++ WCK+TR
Sbjct: 687  MKMEFGESSAANPSQEIVWAKLVPSDSRYSEVELRLDEMVICSDVMFPSFDKQEWCKVTR 746

Query: 258  NSDLCSGTLQNLSSTALLVDGTCVEKDDKAVVKCGTEIISGSDKEGYLSYRFRVMAVPES 437
            NSD CS T+QN SS  +LVD   +  +D  V+KCG+EII G   EGYLSYRF VM+  ES
Sbjct: 747  NSDQCSATIQNKSSNTILVDEVAIPSEDSIVIKCGSEIIPGPKNEGYLSYRFEVMSPQES 806

Query: 438  YWKHLKIPIDSEHAKCSICLHIWHDVVTVAPCLHNFCNGCFSEWLKRSQKENSTIPCPQC 617
              + LKI +D EHAKCSICL+IWHDVVTVAPCLHNFCNGCFSEWL+RSQ ++S + CPQC
Sbjct: 807  IKEQLKICLDVEHAKCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQNKHSNVLCPQC 866

Query: 618  RADVHFVGRNHFLRNIEEGILESDLSLKRSNDELALLDGCASIQSNLVMRTGKLPCRKRP 797
            RA V FVGRNHFLR IEE IL++D SL+RS++E+A+L+  ASIQSNLV++TG    RKR 
Sbjct: 867  RAIVQFVGRNHFLRTIEETILQADPSLRRSDEEVAVLESHASIQSNLVLKTGTKLGRKRA 926

Query: 798  YSPPSEE-ETVELPCLQCGSRFGGFQCNQSTSHLQCQACGGMMPARTDISVPQHCVGCDR 974
            +S  +EE E +E PC QCG+ + GF CN +T HLQC ACGGMMP+R DI VPQHC+GCDR
Sbjct: 927  HSSLNEESEELEFPCPQCGAEYAGFLCNPNTVHLQCHACGGMMPSRNDIGVPQHCLGCDR 986

Query: 975  AFCWAYWRAQGVLPSDSNTVCCRENFKPISGHFVTRIPLLTHQSNRFEQDITERCIQQTG 1154
            AFC AYW  Q    SDS+ VCC E FKPIS H ++RIP L H++N  EQ+ITERCI+Q G
Sbjct: 987  AFCGAYWHTQRFGGSDSHPVCCHETFKPISDHTISRIPTLAHENNWHEQNITERCIRQMG 1046

Query: 1155 KTLQELISDWILKLDKREI 1211
            KTLQ++IS+ +++L  REI
Sbjct: 1047 KTLQDVISELLIRLSNREI 1065



 Score =  154 bits (388), Expect(2) = e-171
 Identities = 67/85 (78%), Positives = 74/85 (87%)
 Frame = +2

Query: 1304 DRTRMPLNHAEMITSGTHVCNECYDKLVSFLLYWFRITTRGFALPPDVSAREDCWYGHAC 1483
            DRTRM LNH+EMIT+ T+VCNECYDKLVSFLLYWFR++     LPPD S REDCWYG+AC
Sbjct: 1066 DRTRMQLNHSEMITASTYVCNECYDKLVSFLLYWFRVSMPKSFLPPDASKREDCWYGYAC 1125

Query: 1484 RTQNHSEEHARKRNHVCRPTRGSGM 1558
            RTQ H+EEHARKRNHVCRPTRGS M
Sbjct: 1126 RTQQHNEEHARKRNHVCRPTRGSNM 1150


>ref|XP_002330437.1| predicted protein [Populus trichocarpa] gi|222871849|gb|EEF08980.1|
            predicted protein [Populus trichocarpa]
          Length = 465

 Score =  479 bits (1232), Expect(2) = e-171
 Identities = 228/372 (61%), Positives = 284/372 (76%), Gaps = 3/372 (0%)
 Frame = +3

Query: 105  SSDKPVTEPWAKLVRLDSKQVDIEIRVDEMVICSENKLLSIGERGWCKITRNSDLCSGTL 284
            S+     E WAKLV  DS+  D+EIR +EMVICSE    S+ +  WCKITRNSD  S  +
Sbjct: 10   SNSSSSNEAWAKLVPSDSRYSDVEIRSNEMVICSEITSTSLEKHEWCKITRNSDQSSAMM 69

Query: 285  QNLSSTALLVDGTCVEKDDKAVVKCGTEIISGSDKEGYLSYRFRVMAVPESYWKHLKIPI 464
            QN S   +LVD   V+ +D  V+ CG+EII G  +EGYLSYRF++M   ES+ + LK+ I
Sbjct: 70   QNKSLNTILVDEATVQNEDDVVINCGSEIIPGPAREGYLSYRFKLMP-RESFTRWLKVSI 128

Query: 465  DSEHAKCSICLHIWHDVVTVAPCLHNFCNGCFSEWLKRSQKENSTIPCPQCRADVHFVGR 644
            D+EHAKCSICL++WHDVVTVAPCLHNFCNGCFSEWL+RSQ+ ++++ CPQCRA V FVGR
Sbjct: 129  DAEHAKCSICLNVWHDVVTVAPCLHNFCNGCFSEWLRRSQERHASVLCPQCRAVVQFVGR 188

Query: 645  NHFLRNIEEGILESDLSLKRSNDELALLDGCASIQSNLVMRTGKLPCRKRPYS-PPSEEE 821
            NHFL +IEE +L++D SLK+S++E+ LLD  ASI+SNL+++TGK   RKRP+S   +E E
Sbjct: 189  NHFLHSIEEDVLQADSSLKQSDEEIILLDSYASIKSNLIIQTGKKHRRKRPHSILDAENE 248

Query: 822  TVELPCLQCGSRFGGFQCNQSTSHLQCQACGGMMPARTDISVPQH--CVGCDRAFCWAYW 995
              + PC QCG+ + GF CNQ+T HLQCQACGGMMP+R DI VPQH  C+GCDR FC AYW
Sbjct: 249  VADFPCPQCGTEYSGFHCNQNTIHLQCQACGGMMPSRADIGVPQHCMCLGCDRVFCGAYW 308

Query: 996  RAQGVLPSDSNTVCCRENFKPISGHFVTRIPLLTHQSNRFEQDITERCIQQTGKTLQELI 1175
             AQGV  SD++++C  ENFKPIS H V+RIP L H+ NR EQDITERCI Q GKTLQ++I
Sbjct: 309  HAQGVSRSDTHSICSHENFKPISEHAVSRIPFLAHEKNRHEQDITERCIGQMGKTLQDVI 368

Query: 1176 SDWILKLDKREI 1211
            SDWI KL+ REI
Sbjct: 369  SDWIGKLNNREI 380



 Score =  152 bits (383), Expect(2) = e-171
 Identities = 64/83 (77%), Positives = 75/83 (90%)
 Frame = +2

Query: 1304 DRTRMPLNHAEMITSGTHVCNECYDKLVSFLLYWFRITTRGFALPPDVSAREDCWYGHAC 1483
            DR+RMPLNHAEMIT GTHVCN+CYDKL+SFLLYWFRI+     LPP+ + REDCWYG+AC
Sbjct: 381  DRSRMPLNHAEMITVGTHVCNDCYDKLISFLLYWFRISMPRNLLPPEAANREDCWYGYAC 440

Query: 1484 RTQNHSEEHARKRNHVCRPTRGS 1552
            RTQ+H+E+HARKRNHVCRPTRG+
Sbjct: 441  RTQHHNEDHARKRNHVCRPTRGN 463


>ref|XP_002268418.1| PREDICTED: E3 ubiquitin-protein ligase CHFR-like [Vitis vinifera]
          Length = 462

 Score =  475 bits (1222), Expect(2) = e-171
 Identities = 228/377 (60%), Positives = 285/377 (75%), Gaps = 3/377 (0%)
 Frame = +3

Query: 90   LEPGESSDKPVTEP--WAKLVRLDSKQVDIEIRVDEMVICSENKLLSIGERGWCKITRNS 263
            +E GESS    ++   WAKLV  DS+  ++E+R+DEMVICS+    S  ++ WCK+TRNS
Sbjct: 1    MEFGESSAANPSQEIVWAKLVPSDSRYSEVELRLDEMVICSDVMFPSFDKQEWCKVTRNS 60

Query: 264  DLCSGTLQNLSSTALLVDGTCVEKDDKAVVKCGTEIISGSDKEGYLSYRFRVMAVPESYW 443
            D CS T+QN SS  +LVD   +  +D  V+KCG+EII G   EGYLSYRF VM+  ES  
Sbjct: 61   DQCSATIQNKSSNTILVDEVAIPSEDSIVIKCGSEIIPGPKNEGYLSYRFEVMSPQESIK 120

Query: 444  KHLKIPIDSEHAKCSICLHIWHDVVTVAPCLHNFCNGCFSEWLKRSQKENSTIPCPQCRA 623
            + LKI +D EHAKCSICL+IWHDVVTVAPCLHNFCNGCFSEWL+RSQ ++S + CPQCRA
Sbjct: 121  EQLKICLDVEHAKCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQNKHSNVLCPQCRA 180

Query: 624  DVHFVGRNHFLRNIEEGILESDLSLKRSNDELALLDGCASIQSNLVMRTGKLPCRKRPYS 803
             V FVGRNHFLR IEE IL++D SL+RS++E+A+L+  ASIQSNLV++TG    RKR +S
Sbjct: 181  IVQFVGRNHFLRTIEETILQADPSLRRSDEEVAVLESHASIQSNLVLKTGTKLGRKRAHS 240

Query: 804  PPSEE-ETVELPCLQCGSRFGGFQCNQSTSHLQCQACGGMMPARTDISVPQHCVGCDRAF 980
              +EE E +E PC QCG+ + GF CN +T HLQC ACGGMMP+R DI VPQHC+GCDRAF
Sbjct: 241  SLNEESEELEFPCPQCGAEYAGFLCNPNTVHLQCHACGGMMPSRNDIGVPQHCLGCDRAF 300

Query: 981  CWAYWRAQGVLPSDSNTVCCRENFKPISGHFVTRIPLLTHQSNRFEQDITERCIQQTGKT 1160
            C AYW  Q    SDS+ VCC E FKPIS H ++RIP L H++N  EQ+ITERCI+Q GKT
Sbjct: 301  CGAYWHTQRFGGSDSHPVCCHETFKPISDHTISRIPTLAHENNWHEQNITERCIRQMGKT 360

Query: 1161 LQELISDWILKLDKREI 1211
            LQ++IS+ +++L  REI
Sbjct: 361  LQDVISELLIRLSNREI 377



 Score =  154 bits (388), Expect(2) = e-171
 Identities = 67/85 (78%), Positives = 74/85 (87%)
 Frame = +2

Query: 1304 DRTRMPLNHAEMITSGTHVCNECYDKLVSFLLYWFRITTRGFALPPDVSAREDCWYGHAC 1483
            DRTRM LNH+EMIT+ T+VCNECYDKLVSFLLYWFR++     LPPD S REDCWYG+AC
Sbjct: 378  DRTRMQLNHSEMITASTYVCNECYDKLVSFLLYWFRVSMPKSFLPPDASKREDCWYGYAC 437

Query: 1484 RTQNHSEEHARKRNHVCRPTRGSGM 1558
            RTQ H+EEHARKRNHVCRPTRGS M
Sbjct: 438  RTQQHNEEHARKRNHVCRPTRGSNM 462


>ref|XP_004146401.1| PREDICTED: E3 ubiquitin-protein ligase CHFR-like [Cucumis sativus]
          Length = 435

 Score =  426 bits (1096), Expect(2) = e-153
 Identities = 204/350 (58%), Positives = 257/350 (73%), Gaps = 2/350 (0%)
 Frame = +3

Query: 168  DIEIRVDEMVICSENKLLSIGERGWCKITRNSDLCSGTLQNLSSTALLVDGTCVEKDDKA 347
            DIEI  DE VI SE K  S+ +  WCKITRNSDL + TLQN SS A++VD T V+KD+  
Sbjct: 4    DIEINSDETVIFSETKSTSVEKHEWCKITRNSDLNTATLQNRSSNAIIVDETLVQKDETT 63

Query: 348  VVKCGTEIISGSDKEGYLSYRFRVMAVPESYWKHLKIPIDSEHAKCSICLHIWHDVVTVA 527
             +KCG+EI+SG  ++G LS++F +++      K LKI +D EHAKCSICL+IWHDVVT A
Sbjct: 64   FIKCGSEIVSGPVRDGNLSFKFEMLS-DSKLCKGLKISVDVEHAKCSICLNIWHDVVTAA 122

Query: 528  PCLHNFCNGCFSEWLKRSQKENSTIPCPQCRADVHFVGRNHFLRNIEEGILESDLSLKRS 707
            PC HNFCNGCFSEWLKRSQ++++ + CPQCRA V FVGRNH+L NI E IL++D SL+RS
Sbjct: 123  PCFHNFCNGCFSEWLKRSQEKHTNVLCPQCRAVVQFVGRNHYLLNIAEDILQADSSLRRS 182

Query: 708  NDELALLDGCASIQSNLVMRTGKLPCRKRPYSPPSEEET--VELPCLQCGSRFGGFQCNQ 881
             +E+A LD C+ IQSNLV+ + K   +KRP+     EE+  +ELPC QCG+ + GF+CN 
Sbjct: 183  VEEIATLDSCSLIQSNLVIGSVKKRNQKRPHFLLGLEESGGLELPCPQCGTEYAGFRCNP 242

Query: 882  STSHLQCQACGGMMPARTDISVPQHCVGCDRAFCWAYWRAQGVLPSDSNTVCCRENFKPI 1061
               HLQC  CGGMMP+R D  +PQHC+GCDRAFC AYW AQ +  SD    C  E FKPI
Sbjct: 243  HMVHLQCHECGGMMPSRFDTGIPQHCLGCDRAFCGAYWEAQSLQRSDIYATCSPETFKPI 302

Query: 1062 SGHFVTRIPLLTHQSNRFEQDITERCIQQTGKTLQELISDWILKLDKREI 1211
            S   ++RIP LTH++NR EQDIT RCI Q GKTLQ++IS+WI++   REI
Sbjct: 303  SRRTISRIPQLTHENNRHEQDITGRCISQMGKTLQDVISEWIVRFSNREI 352



 Score =  142 bits (359), Expect(2) = e-153
 Identities = 60/81 (74%), Positives = 72/81 (88%)
 Frame = +2

Query: 1304 DRTRMPLNHAEMITSGTHVCNECYDKLVSFLLYWFRITTRGFALPPDVSAREDCWYGHAC 1483
            DRTRMPLNHAEMIT+ T +C+ECY+KLV FLLYWFRI+T  + LP D + RE+CWYG+AC
Sbjct: 353  DRTRMPLNHAEMITAETPICHECYEKLVGFLLYWFRISTPKYLLPEDATERENCWYGYAC 412

Query: 1484 RTQNHSEEHARKRNHVCRPTR 1546
            RTQ+H+E+HARKRNHVCRPTR
Sbjct: 413  RTQHHNEDHARKRNHVCRPTR 433


>ref|XP_003630425.1| E3 ubiquitin-protein ligase CHFR [Medicago truncatula]
            gi|355524447|gb|AET04901.1| E3 ubiquitin-protein ligase
            CHFR [Medicago truncatula]
          Length = 471

 Score =  425 bits (1092), Expect(2) = e-152
 Identities = 211/372 (56%), Positives = 269/372 (72%), Gaps = 3/372 (0%)
 Frame = +3

Query: 105  SSDKPVTEPWAKLVRLDSKQVDIEIRVDEMVICSENKLLSIGERGWCKITRNSDLCSGTL 284
            +S KP  E WAKLV+ DS+  D+EIR D+  I SE    S  +  WCKI RNSDLCS TL
Sbjct: 16   ASAKPSDEIWAKLVQSDSRYPDVEIRSDKGQIRSEISTASCDKHRWCKIVRNSDLCSATL 75

Query: 285  QNLSSTALLVDGTCVEKDDKAVVKCGTEIISGSDKEGYLSYRFRVMAVPESYWKHLKIPI 464
            +N SS  +LVDG  V K D  V+K  +EII G  KEG++SY+F++++ PE     LKI +
Sbjct: 76   KNKSSNTILVDGAEVGKGDTVVIKDRSEIIPGPAKEGFVSYKFQIVSSPEICQTLLKICV 135

Query: 465  DSEHAKCSICLHIWHDVVTVAPCLHNFCNGCFSEWLKRSQKENSTIPCPQCRADVHFVGR 644
            D +HAKCSICL+IWHDVVTVAPCLHNFCNGCFSEWL+RSQ++ ST+ CPQCRA V FVG+
Sbjct: 136  DVDHAKCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQFVGK 195

Query: 645  NHFLRNIEEGILESDLSLKRSNDELALLDGCASIQSNLVMRTGKLPCRKRPYSPPSEEET 824
            NHFLR I E ++++D SL+RS+DE+ALLD  AS++SNLV+ +GK   RKR  +  +    
Sbjct: 196  NHFLRTIAEDMVKADSSLRRSHDEVALLDTYASVRSNLVIGSGK-KSRKRERANTTMVNQ 254

Query: 825  VELP---CLQCGSRFGGFQCNQSTSHLQCQACGGMMPARTDISVPQHCVGCDRAFCWAYW 995
             + P   C QC +  GGFQC+ ST HLQCQ CGGMMP+RT + VPQ+C+GCDR FC AYW
Sbjct: 255  SDGPDHHCSQCVTVVGGFQCDPSTIHLQCQECGGMMPSRTGLGVPQYCLGCDRPFCGAYW 314

Query: 996  RAQGVLPSDSNTVCCRENFKPISGHFVTRIPLLTHQSNRFEQDITERCIQQTGKTLQELI 1175
             A GV  S S  VC  +  +PIS H ++ IPL+ H+ N  EQ+ITE CI+Q G+TLQ++I
Sbjct: 315  SALGVTGSGSYPVCSPDTLRPISEHTISGIPLVAHEKNLHEQNITESCIRQMGRTLQDVI 374

Query: 1176 SDWILKLDKREI 1211
            S+WI KL+ REI
Sbjct: 375  SEWIAKLNNREI 386



 Score =  142 bits (358), Expect(2) = e-152
 Identities = 62/83 (74%), Positives = 71/83 (85%)
 Frame = +2

Query: 1304 DRTRMPLNHAEMITSGTHVCNECYDKLVSFLLYWFRITTRGFALPPDVSAREDCWYGHAC 1483
            D TR+ LNHAEM+T+GT VC++CY KLVSF LYWFRI+T    LPP+ S REDCWYG+AC
Sbjct: 387  DTTRLMLNHAEMMTAGTLVCSDCYQKLVSFFLYWFRISTPKHLLPPEASTREDCWYGYAC 446

Query: 1484 RTQNHSEEHARKRNHVCRPTRGS 1552
            RTQ+ SEEHARKRNHVCRPTRGS
Sbjct: 447  RTQHRSEEHARKRNHVCRPTRGS 469