BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00003227
         (2916 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265386.1| PREDICTED: DNA topoisomerase 1-like [Vitis v...  1065   0.0  
dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]              1045   0.0  
dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]                1019   0.0  
emb|CBI28783.3| unnamed protein product [Vitis vinifera]             1010   0.0  
ref|XP_004149585.1| PREDICTED: DNA topoisomerase 1-like [Cucumis...   996   0.0  

>ref|XP_002265386.1| PREDICTED: DNA topoisomerase 1-like [Vitis vinifera]
          Length = 883

 Score = 1065 bits (2753), Expect = 0.0
 Identities = 562/882 (63%), Positives = 635/882 (71%), Gaps = 12/882 (1%)
 Frame = -3

Query: 2893 PVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPLRKQASDALSPNGQKSTIQQGKEGSSSKA 2714
            PV    S+ +     LSA+++ +    +   L+  +S  L     K  +++    S  + 
Sbjct: 10   PVANGESEDSEDDKPLSAKLSARLPKGNSHVLKGLSSPVLQSQKTKVDVKKSDGDSEDEV 69

Query: 2713 TAAKKHVTSSNDSTLVADKSIHLGTTLGNNNLEKKYLNKRHAEPT----SDSEDSDDKKP 2546
              + K        T V   S   G +LG+  L    L+    E        S+DS+D+ P
Sbjct: 70   PLSSKFPLK----TYVRSSS---GKSLGSKGLNSPSLSSHPPETKVGVKESSDDSEDEIP 122

Query: 2545 LSFRLPAGLAAPPKSNSPSVLKGLTXXXXXXXXSEKPKVSVK--SEDSEDDMPLSSKFHL 2372
            L  +L         S+     KGL+        S+K KV+ K  S+DSED++PLSSKF L
Sbjct: 123  LLLKLQLKSRKGASSSKLLDSKGLSSPASSRPQSQKAKVNNKNLSDDSEDEIPLSSKFRL 182

Query: 2371 KSGAGASVKKPYDSDEDKPLSSKIQQNGNVKRDNESSQTPKNSNKRPLGDASTSAQXXXX 2192
            K+G GAS+ K  + DE KPL+S ++ NG  K+D+E  ++PK  NKRPLG A+TS      
Sbjct: 183  KTGMGASLDKLDNFDEKKPLTSTLKPNGISKKDDERVKSPKGQNKRPLGQANTSHHSSSK 242

Query: 2191 XXXXXXXXXXXXXXXAFVKAEPXXXXXXXD------VPIAQRMTKSATSDSKSKPMKQTP 2030
                             VKAE        +       P  +R+ KS +SD+K K  K   
Sbjct: 243  KPKLLDGSVKGKVKQVSVKAEQKDDDDDDNDDDDEYTPTVKRLKKSVSSDNK-KASKTKV 301

Query: 2029 XXXXXXXXXXXXXXXXXXXXXXKYLKSTKTPPGSGEGQKWTTLVHNGVIFPPPYKPHGVK 1850
                                  KY KSTK PP SGEGQKWTTL HNGVIFPPPYKPHGVK
Sbjct: 302  IPSSRKKATKKAKKSKKLAKSSKYSKSTKVPPNSGEGQKWTTLEHNGVIFPPPYKPHGVK 361

Query: 1849 MIYNGKPVDLTPEQEEVATMFAVMKDTDYATKPKFLENFMTDWRTVLGKNHIIKKFELCD 1670
            M+YNG+PVDLTPEQEEVATMFAVMKDTDYATKP+F+ENFM DWR  LGKNH+IKKFELCD
Sbjct: 362  MLYNGRPVDLTPEQEEVATMFAVMKDTDYATKPRFIENFMNDWRVRLGKNHVIKKFELCD 421

Query: 1669 FTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXXXXKYMWAIVDGVKEKVGNFRVEPPGLF 1490
            FTPIYEWH+ EKEKKKQM+T               KYMWA+VDGVKEKVGNFRVEPPGLF
Sbjct: 422  FTPIYEWHEKEKEKKKQMTTEEKKALKEEKLKQEDKYMWAVVDGVKEKVGNFRVEPPGLF 481

Query: 1489 RGRGEHPKMGKLKKRIRPRDITINIGKGAPVPECSIPGERWKEVKHDNTVTWLAFWNDPI 1310
            RGRGEHPKMGKLKKRI P DITINIGK APVPEC IPGERWKEV+HDNTVTWLAFWNDPI
Sbjct: 482  RGRGEHPKMGKLKKRICPSDITINIGKEAPVPECPIPGERWKEVRHDNTVTWLAFWNDPI 541

Query: 1309 NPKEFKYVFLAASSSLKGQSDKEKYEKARLLKEYIENIRVNYAKDFTNKDVVKRQVAVAT 1130
            NPKEFKYVFLAASSSLKGQSDKEKYEKARLLK+YI+NIR  Y KDFTNKD +KRQ+AVAT
Sbjct: 542  NPKEFKYVFLAASSSLKGQSDKEKYEKARLLKDYIQNIRATYTKDFTNKDTMKRQIAVAT 601

Query: 1129 YLIDKLALRAGNEKDDDEADTVGCCTLKVENVKLVPPNKLEFDFLGKDSVRYFNTVDVEV 950
            YLIDKLALRAGNEKDDDEADTVGCCTLKVENV LVPPNKLEFDFLGKDS+RYFNTV+VE+
Sbjct: 602  YLIDKLALRAGNEKDDDEADTVGCCTLKVENVALVPPNKLEFDFLGKDSIRYFNTVEVEL 661

Query: 949  PVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTFNASITLDDMLNKE 770
            PVYKAIGQFK+GK  GDDLFDKLDTSKLNAHLKELMPGLTAKVFRT+NASITLD+MLN+E
Sbjct: 662  PVYKAIGQFKTGKSGGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTYNASITLDEMLNRE 721

Query: 769  TRGGDIAQKIVVYQHANKEVAIICNHQRSVSKSHSAQMDKLTEKMNXXXXXXXXXXXXXA 590
             RGGD  +KIV+YQHANKEVAIICNHQRSVSKSH  QM +L+EKM              A
Sbjct: 722  ARGGDAMEKIVIYQHANKEVAIICNHQRSVSKSHDVQMSRLSEKMRELQGVLDELKTDLA 781

Query: 589  RARKGKPPLKDADGKTKKNLTPEALERKIAQTNTKIEKMERDRQTKEDLKTVALGTSKIN 410
            RA+KGKPPLKD+DGK KKN+TPE LERKIAQTNTKIEKMERD +TKEDLKTVALGTSKIN
Sbjct: 782  RAKKGKPPLKDSDGKPKKNMTPEVLERKIAQTNTKIEKMERDMKTKEDLKTVALGTSKIN 841

Query: 409  YLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAMDVEPDFRF 284
            YLDPRISVAWCKR EVPIEKIFNKSLLAKFAWAMDV+P FRF
Sbjct: 842  YLDPRISVAWCKRQEVPIEKIFNKSLLAKFAWAMDVDPSFRF 883


>dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]
          Length = 894

 Score = 1045 bits (2702), Expect = 0.0
 Identities = 553/887 (62%), Positives = 644/887 (72%), Gaps = 17/887 (1%)
 Frame = -3

Query: 2893 PVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPLRKQASDALSPNGQKSTIQQGKEGSSSKA 2714
            P+VFKRS  T+K NQL+++  +KSS + D    +Q SD  S NGQ S+  +GK   ++K 
Sbjct: 21   PLVFKRSNATSKHNQLNSDT-KKSSQRYDGQSGRQVSDVPSTNGQSSSASKGK---TTKL 76

Query: 2713 TAAKKHVTSSNDSTLVA---------------DKSIHLGTTLGNNNL-EKKYLNKRHAEP 2582
            ++ K  + S   ST  A               D+S H       N + E+K L    AEP
Sbjct: 77   SSMKSPIASPKASTSFAKASPVANPKVSSSSDDRSKHSSKQNTINVVKEEKELVNPAAEP 136

Query: 2581 TSDSEDSDDKKPLSFRLPAGLAAPPKSNSPSVLKGLTXXXXXXXXSEKPKVSVKSEDSED 2402
              DSED   +KPLS RL  GL    K +S +  KGL           KP+++  S+DS+D
Sbjct: 137  YGDSED---EKPLSARLFTGLT---KGSSNNANKGLINSSPASLPVPKPEINRYSDDSDD 190

Query: 2401 DMPLSSKFHLKSGAGASVKKPYDSDEDKPLSSKIQQNGNVKRDNESSQTPKNSNKRPLGD 2222
            ++PLSSKF LK+ AG S  K Y+SD++K L S  QQNG++ R ++SS      NKRPLG+
Sbjct: 191  EIPLSSKFRLKANAGTSTVKSYESDDNKTLVSNFQQNGSINRGSKSSI---KVNKRPLGE 247

Query: 2221 ASTSAQXXXXXXXXXXXXXXXXXXXAFVKAEPXXXXXXXD-VPIAQRMTKSATSDSKSKP 2045
              +S Q                   A  KAEP       D VPI+QRM KSATS +KS  
Sbjct: 248  VKSSVQSSVKKPKLSDASTPVNNKQASKKAEPKADDSDDDHVPISQRMKKSATSGNKSST 307

Query: 2044 MKQTPXXXXXXXXXXXXXXXXXXXXXXKYLKSTKTPPGSGEGQKWTTLVHNGVIFPPPYK 1865
             KQ                        KY KS+K PP SGEG+KW TLVHNGVIFPPPYK
Sbjct: 308  AKQKVTKVVSSSMKKASKKFKKVVKKSKYSKSSKVPPSSGEGKKWNTLVHNGVIFPPPYK 367

Query: 1864 PHGVKMIYNGKPVDLTPEQEEVATMFAVMKDTDYATKPKFLENFMTDWRTVLGKNHIIKK 1685
            PHGVKM+Y GKPVDLTPEQEEVATMFAVM DTDY TK KF ENFM DWR +LGKNH+I+ 
Sbjct: 368  PHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKSKFKENFMDDWRKILGKNHVIQN 427

Query: 1684 FELCDFTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXXXXKYMWAIVDGVKEKVGNFRVE 1505
             E CDFTPIYEWHQ EKEKKK+M+                KYMWAIV+GVKEKVGNFRVE
Sbjct: 428  LEDCDFTPIYEWHQREKEKKKKMTAEEKKVLKEERLKQEEKYMWAIVNGVKEKVGNFRVE 487

Query: 1504 PPGLFRGRGEHPKMGKLKKRIRPRDITINIGKGAPVPECSIPGERWKEVKHDNTVTWLAF 1325
            PP LFRGRGEHPKMGKLKK IRP DITINIGK AP+PEC IPGE WKE++HDNTVTWLAF
Sbjct: 488  PPSLFRGRGEHPKMGKLKKCIRPSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAF 547

Query: 1324 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKEYIENIRVNYAKDFTNKDVVKRQ 1145
            WNDPI P+EFKYVFLAASSSLKGQSDKEKYEKARLLK++I+ IR  Y KDF +KD+ KRQ
Sbjct: 548  WNDPIKPREFKYVFLAASSSLKGQSDKEKYEKARLLKDFIQGIRAAYTKDFASKDITKRQ 607

Query: 1144 VAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKLVPPNKLEFDFLGKDSVRYFNT 965
            +AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+  PP+ L+FDFLGKDS+RY N 
Sbjct: 608  IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPKPPSILKFDFLGKDSIRYQNE 667

Query: 964  VDVEVPVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTFNASITLDD 785
            V+VE+PVYKAI QF++GK+ GDDLFDKLDTSKLNAHLK LMPGLTAKVFRT+NASITLD+
Sbjct: 668  VEVELPVYKAIQQFRTGKRGGDDLFDKLDTSKLNAHLKGLMPGLTAKVFRTYNASITLDE 727

Query: 784  MLNKETRGGDIAQKIVVYQHANKEVAIICNHQRSVSKSHSAQMDKLTEKMNXXXXXXXXX 605
            ML++ET+GG++A+KIVVYQHANKEVAIICNHQR+VSKSH AQM +L  K++         
Sbjct: 728  MLSRETKGGNVAEKIVVYQHANKEVAIICNHQRTVSKSHPAQMTRLNGKIDELKGILDGL 787

Query: 604  XXXXARARKGKPPLKDADGKTKKNLTPEALERKIAQTNTKIEKMERDRQTKEDLKTVALG 425
                ARA+KGKPPL+DADGK K+NLTPEALERKI QTN KIEKMERD++TKE LKT+ALG
Sbjct: 788  QTDLARAKKGKPPLEDADGKPKRNLTPEALERKIGQTNAKIEKMERDKETKEGLKTIALG 847

Query: 424  TSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAMDVEPDFRF 284
            TSKI+YLDPRI+VAWCKRHEVPIEK+FNKSLLAKFAW+MDV+P FRF
Sbjct: 848  TSKISYLDPRITVAWCKRHEVPIEKVFNKSLLAKFAWSMDVDPSFRF 894


>dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]
          Length = 910

 Score = 1019 bits (2635), Expect = 0.0
 Identities = 543/908 (59%), Positives = 635/908 (69%), Gaps = 38/908 (4%)
 Frame = -3

Query: 2893 PVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPLRKQASDALSPNGQKSTIQQGK------- 2735
            P+VFKR+ + +KQ+Q ++E  + SS K D    +Q  D+   NG+ S+ Q+G+       
Sbjct: 21   PIVFKRNNSASKQSQTNSETRKVSSQKRDGQSVRQIYDSRPANGESSSGQKGRMVPPSKP 80

Query: 2734 -------EGSSSKATAAKKHVTSSNDSTLVADKS-------IHLGTTLG-------NNNL 2618
                       SK +  K  ++S   ST  A KS           +T G       N + 
Sbjct: 81   SQVKSPLSSPPSKPSQVKSPLSSPKVSTSSAKKSPVSSLPANSKPSTSGSFKVKPFNQHK 140

Query: 2617 EKKYLNKRHAEP-------TSDSEDSDDKKPLSFRLPAGLAAPPKSNSPSVLKGLTXXXX 2459
                + +  + P        +DSEDSDD KPLS R     A+  K N+  V K       
Sbjct: 141  SATVIKEEKSSPLRLASAIINDSEDSDDGKPLSLR---HSASSSKGNTNQVSK------- 190

Query: 2458 XXXXSEKPKVSVKSEDSEDDMPLSSKFHLKSGAGASVKKPYDSDEDKPLSSKIQQNGNVK 2279
                  KP    ++EDS+D+ PLSS+F LKSG G S  K YDSD+DKPL+ K+Q NG+  
Sbjct: 191  ---EGSKP----ENEDSDDEKPLSSRFPLKSGVGESTSKAYDSDDDKPLAKKLQHNGSAM 243

Query: 2278 RDNESSQTPKNSNKRPLGDASTSAQXXXXXXXXXXXXXXXXXXXAFVKAEP-XXXXXXXD 2102
            RD + ++    SNKRP GD  +S Q                   A VK EP         
Sbjct: 244  RDGQPNKYSNMSNKRPPGDIKSSNQLSVKKPKLSSVTAPTNKKQASVKPEPKAEDDDDDH 303

Query: 2101 VPIAQRMTKSATSDSKSKPMKQTPXXXXXXXXXXXXXXXXXXXXXXKYLKSTKTPPGSGE 1922
            +PI+QR+ KS TS+SKS  +K+T                       KY KS+K PP SGE
Sbjct: 304  LPISQRIKKSPTSESKSSSIKKT-TKVVSSSVKKVNKKSKKVMKNSKYSKSSKVPPSSGE 362

Query: 1921 GQKWTTLVHNGVIFPPPYKPHGVKMIYNGKPVDLTPEQEEVATMFAVMKDTDYATKPKFL 1742
            G+KW  L HNGVIFPPPYKPHG+KM+Y GKPVDLTPEQEEVATMFAVM DTDY  KP+F 
Sbjct: 363  GKKWNKLEHNGVIFPPPYKPHGIKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMNKPRFK 422

Query: 1741 ENFMTDWRTVLGKNHIIKKFELCDFTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXXXXK 1562
            ENF  DW+ +LGKNH+I+  E CDF+PIYEWHQ+EKEKKKQM+T               K
Sbjct: 423  ENFFGDWKKILGKNHVIQNLENCDFSPIYEWHQSEKEKKKQMTTEEKKALKEEKLKQEEK 482

Query: 1561 YMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKGAPVPECSI 1382
            YMWA VDG KEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRP DITINIGK AP+PEC +
Sbjct: 483  YMWAWVDGKKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPCDITINIGKDAPIPECPV 542

Query: 1381 PGERWKEVKHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKEYIE 1202
            PGERWKEV+HDNTVTWLAFWNDPINPKEFKYVFLAASS+LKG SDKEKYEKARLLK+YI+
Sbjct: 543  PGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSTLKGLSDKEKYEKARLLKDYIQ 602

Query: 1201 NIRVNYAKDFTNKDVVKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKLVP 1022
             IR  Y KDF +KD  K+Q+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ VP
Sbjct: 603  GIRAAYTKDFASKDPTKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVP 662

Query: 1021 PNKLEFDFLGKDSVRYFNTVDVEVPVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLKELM 842
            PN L+FDFLGKDS+RY N V+VE  V+KAI QF+SGK+   DLFD LDTSKLNAHLKELM
Sbjct: 663  PNILKFDFLGKDSIRYQNEVEVEAAVFKAIQQFRSGKEGSSDLFDLLDTSKLNAHLKELM 722

Query: 841  PGLTAKVFRTFNASITLDDMLNKETRGGDIAQKIVVYQHANKEVAIICNHQRSVSKSHSA 662
            PGLTAKVFRT+NASITLDDML++ET+GGD+A+K+VVYQHANKEVAIICNHQR+VSKSHSA
Sbjct: 723  PGLTAKVFRTYNASITLDDMLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSA 782

Query: 661  QMDKLTEKMNXXXXXXXXXXXXXARARKGKPPL--KDADGKTKKNLTPEALERKIAQTNT 488
            QM +L EK+              +R +KGKPPL  K+ADGK K+NL PE+LE+KIAQTN 
Sbjct: 783  QMLRLNEKIEELKAVVEELKSDLSRVKKGKPPLKSKNADGKPKRNLNPESLEKKIAQTNA 842

Query: 487  KIEKMERDRQTKEDLKTVALGTSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAM 308
            KIEKMERD++TKEDLKTVALGTSKINYLDPRI+VAWCKRHEVPIEKIFNKSLLAKF WAM
Sbjct: 843  KIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFTWAM 902

Query: 307  DVEPDFRF 284
            DV+P FRF
Sbjct: 903  DVDPSFRF 910


>emb|CBI28783.3| unnamed protein product [Vitis vinifera]
          Length = 724

 Score = 1010 bits (2611), Expect = 0.0
 Identities = 529/782 (67%), Positives = 582/782 (74%), Gaps = 9/782 (1%)
 Frame = -3

Query: 2602 NKRHAEP---TSDSEDSDDKKPLSFRLPAGLAAPPKSNSPSVLKGLTXXXXXXXXSEKPK 2432
            NK+   P     +SEDS+D KPLS +L A L   PK NS  VLKGL+         +K K
Sbjct: 3    NKQLINPPVANGESEDSEDDKPLSAKLSARL---PKGNS-HVLKGLSSPVLQS---QKTK 55

Query: 2431 VSVKSEDSEDDMPLSSKFHLKSGAGASVKKPYDSD------EDKPLSSKIQQNGNVKRDN 2270
            V   S+DSED++PL  K  LKS  GAS  K  DS         +P S K + N     D+
Sbjct: 56   VD-SSDDSEDEIPLLLKLQLKSRKGASSSKLLDSKGLSSPASSRPQSQKAKVNNKNLSDD 114

Query: 2269 ESSQTPKNSNKRPLGDASTSAQXXXXXXXXXXXXXXXXXXXAFVKAEPXXXXXXXDVPIA 2090
               + P +S  R                               V ++         +P +
Sbjct: 115  SEDEIPLSSKFR----------------------------LKTVSSDNKKASKTKVIPSS 146

Query: 2089 QRMTKSATSDSKSKPMKQTPXXXXXXXXXXXXXXXXXXXXXXKYLKSTKTPPGSGEGQKW 1910
            ++  K+     KSK + ++                        Y KSTK PP SGEGQKW
Sbjct: 147  RK--KATKKAKKSKKLAKSSK----------------------YSKSTKVPPNSGEGQKW 182

Query: 1909 TTLVHNGVIFPPPYKPHGVKMIYNGKPVDLTPEQEEVATMFAVMKDTDYATKPKFLENFM 1730
            TTL HNGVIFPPPYKPHGVKM+YNG+PVDLTPEQEEVATMFAVMKDTDYATKP+F+ENFM
Sbjct: 183  TTLEHNGVIFPPPYKPHGVKMLYNGRPVDLTPEQEEVATMFAVMKDTDYATKPRFIENFM 242

Query: 1729 TDWRTVLGKNHIIKKFELCDFTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXXXXKYMWA 1550
             DWR  LGKNH+IKKFELCDFTPIYEWH+ EKEKKKQM+T               KYMWA
Sbjct: 243  NDWRVRLGKNHVIKKFELCDFTPIYEWHEKEKEKKKQMTTEEKKALKEEKLKQEDKYMWA 302

Query: 1549 IVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKGAPVPECSIPGER 1370
            +VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI P DITINIGK APVPEC IPGER
Sbjct: 303  VVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRICPSDITINIGKEAPVPECPIPGER 362

Query: 1369 WKEVKHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKEYIENIRV 1190
            WKEV+HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLK+YI+NIR 
Sbjct: 363  WKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKDYIQNIRA 422

Query: 1189 NYAKDFTNKDVVKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKLVPPNKL 1010
             Y KDFTNKD +KRQ+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV LVPPNKL
Sbjct: 423  TYTKDFTNKDTMKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVALVPPNKL 482

Query: 1009 EFDFLGKDSVRYFNTVDVEVPVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLKELMPGLT 830
            EFDFLGKDS+RYFNTV+VE+PVYKAIGQFK+GK  GDDLFDKLDTSKLNAHLKELMPGLT
Sbjct: 483  EFDFLGKDSIRYFNTVEVELPVYKAIGQFKTGKSGGDDLFDKLDTSKLNAHLKELMPGLT 542

Query: 829  AKVFRTFNASITLDDMLNKETRGGDIAQKIVVYQHANKEVAIICNHQRSVSKSHSAQMDK 650
            AKVFRT+NASITLD+MLN+E RGGD  +KIV+YQHANKEVAIICNHQRSVSKSH  QM +
Sbjct: 543  AKVFRTYNASITLDEMLNREARGGDAMEKIVIYQHANKEVAIICNHQRSVSKSHDVQMSR 602

Query: 649  LTEKMNXXXXXXXXXXXXXARARKGKPPLKDADGKTKKNLTPEALERKIAQTNTKIEKME 470
            L+EKM              ARA+KGKPPLKD+DGK KKN+TPE LERKIAQTNTKIEKME
Sbjct: 603  LSEKMRELQGVLDELKTDLARAKKGKPPLKDSDGKPKKNMTPEVLERKIAQTNTKIEKME 662

Query: 469  RDRQTKEDLKTVALGTSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAMDVEPDF 290
            RD +TKEDLKTVALGTSKINYLDPRISVAWCKR EVPIEKIFNKSLLAKFAWAMDV+P F
Sbjct: 663  RDMKTKEDLKTVALGTSKINYLDPRISVAWCKRQEVPIEKIFNKSLLAKFAWAMDVDPSF 722

Query: 289  RF 284
            RF
Sbjct: 723  RF 724


>ref|XP_004149585.1| PREDICTED: DNA topoisomerase 1-like [Cucumis sativus]
            gi|449503189|ref|XP_004161878.1| PREDICTED: DNA
            topoisomerase 1-like [Cucumis sativus]
          Length = 889

 Score =  996 bits (2575), Expect = 0.0
 Identities = 532/886 (60%), Positives = 627/886 (70%), Gaps = 16/886 (1%)
 Frame = -3

Query: 2893 PVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPLRKQASDALSPNGQKSTIQQGKEGSSSKA 2714
            P+VFKR+ + ++QNQL +E+    + +      +Q SD  S NGQ S  Q+ +  S  K 
Sbjct: 21   PIVFKRT-SASRQNQLDSEIKHSQAQRLSKLPGRQGSDLCSTNGQSSNNQK-RMTSLPKT 78

Query: 2713 TAAKKHVTS---SNDSTLVADKSIHLGTTL----GNNNLEKKYLNKRHAE------PTSD 2573
            +  +  + S   S  S +    +  LGT+L      ++  K  +N    E      P+ +
Sbjct: 79   SPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPKSLVNAVVKEENVSINPSQE 138

Query: 2572 SEDSDDKKPLSFRLPAGLAAPPKSNSPSVLKGLTXXXXXXXXSEKPKVSVKSEDSEDDMP 2393
            + DS+D KPLS RL        K N     KGL           K KV     DS+D++P
Sbjct: 139  NSDSEDDKPLSIRL--------KGNPNQPNKGLVSTVP------KNKVKKSLADSDDEVP 184

Query: 2392 LSSKFHLKSGAGASVKKPYDSDEDKPLSSKIQQNGNVKRDNESSQTPKNSNKRPLG--DA 2219
            LSSKF +K  +G    K +  DE KPL  K+ QNG+  RD +  +    S+KRPL   D+
Sbjct: 185  LSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRD-KLQKPAVLSSKRPLPNEDS 243

Query: 2218 STSAQXXXXXXXXXXXXXXXXXXXAFVKAEPXXXXXXXDVPIAQRMTKSATSDSKSKPMK 2039
             + +Q                     +KA+        D+PI+QRM K + + +KS   K
Sbjct: 244  HSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKSSSSK 303

Query: 2038 QTPXXXXXXXXXXXXXXXXXXXXXXKYLKSTKTPPGSGEGQK-WTTLVHNGVIFPPPYKP 1862
             T                       KY+KSTK  P +G+GQK WTTLVHNGVIFPPPY+ 
Sbjct: 304  PTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQT 363

Query: 1861 HGVKMIYNGKPVDLTPEQEEVATMFAVMKDTDYATKPKFLENFMTDWRTVLGKNHIIKKF 1682
            HGVKM+Y GKPVDLTP QEEVATMFAVMKDTDY  KPKF ENF  DWR +LGKNH+I+  
Sbjct: 364  HGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNL 423

Query: 1681 ELCDFTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXXXXKYMWAIVDGVKEKVGNFRVEP 1502
            + CDFTPIY+W+Q EKEKKKQM+T               KYMWAI+DGVKEKVGNFRVEP
Sbjct: 424  DDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEKVGNFRVEP 483

Query: 1501 PGLFRGRGEHPKMGKLKKRIRPRDITINIGKGAPVPECSIPGERWKEVKHDNTVTWLAFW 1322
            PGLFRGRGEHPKMGKLK+RI P DITINIGKGAP+PEC IPGERWKEV+HDNTVTWLAFW
Sbjct: 484  PGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFW 543

Query: 1321 NDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKEYIENIRVNYAKDFTNKDVVKRQV 1142
            NDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR+LK+YI+NIR  Y KDFT+KD  +RQ+
Sbjct: 544  NDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQI 603

Query: 1141 AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKLVPPNKLEFDFLGKDSVRYFNTV 962
            AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPN LEF+FLGKDS+RY NTV
Sbjct: 604  AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTV 663

Query: 961  DVEVPVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTFNASITLDDM 782
            +VE+PV+KAI QF+SGK   DDLFDKLDTSKLNAHLK+LMPGLTAKVFRT+NASITLDDM
Sbjct: 664  EVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDM 723

Query: 781  LNKETRGGDIAQKIVVYQHANKEVAIICNHQRSVSKSHSAQMDKLTEKMNXXXXXXXXXX 602
            LN+ T+ G++++KIV YQHANKEVAIICNHQR+VSKSH AQM +L EK++          
Sbjct: 724  LNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELK 783

Query: 601  XXXARARKGKPPLKDADGKTKKNLTPEALERKIAQTNTKIEKMERDRQTKEDLKTVALGT 422
                RA+KGKPPLK +DGK K+NLTPEALE+KI+QTN KIEK+ERD +TKEDLKTVALGT
Sbjct: 784  IDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGT 843

Query: 421  SKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAMDVEPDFRF 284
            SKINYLDPRI+VAWCKRHEVPIEKIFNKSLLAKFAWAMDV+PDFRF
Sbjct: 844  SKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889