BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00003171
         (1887 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]       382   e-103
ref|XP_002263652.1| PREDICTED: secologanin synthase-like [Vitis ...   341   5e-91
gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago ...   335   2e-89
dbj|BAL45198.1| cytochrome P450 monooxygenase [Medicago truncatula]   335   2e-89
emb|CBI39912.3| unnamed protein product [Vitis vinifera]              335   2e-89

>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 518

 Score =  382 bits (982), Expect = e-103
 Identities = 185/268 (69%), Positives = 213/268 (79%)
 Frame = +3

Query: 873  PTKRNNRMKEIYREVKTLLGDMIKKREKAMKLGEPSTDDLLGLLMESNHKEIKDGGNAKN 1052
            PTKRNNRMKEIY E + LL D++ KREKAMK+GE   DDLLGLLMESN KEI D  N+KN
Sbjct: 251  PTKRNNRMKEIYGEARVLLRDIVNKREKAMKIGEAHNDDLLGLLMESNFKEITDNVNSKN 310

Query: 1053 AGMSIDEVIEECKLFYIAGQETTSTLLLWTMVVLCMHPEWQEKAREEVLQVFGQNKPDLE 1232
             GM+IDEVI+ECKLFY AGQETTSTLL+WTMVVL MHP+WQEKAREEVLQVFG   PD +
Sbjct: 311  FGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFD 370

Query: 1233 GLNHLKIVTMILYEVLRLYPPVVMYSRATYKAIKLGEICIXXXXXXXXXXXXXHHDRDLW 1412
            GLNHLKIVTMILYEVLRLYPP V+ +R TYK +KLGEI +             HHDR+LW
Sbjct: 371  GLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELW 430

Query: 1413 GEDAEEFKPERFVEGISKATKNRVSFFPFSYGPRICIGQSFXXXXXXXXXXXXLQNFSFQ 1592
            GEDAE+F PERF  G+SKATKN+VSFFPFS+GPRICIGQ+F            LQ FSF+
Sbjct: 431  GEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFE 490

Query: 1593 LSATYAHAPFSFLTIQPQYGAQLILHKV 1676
            LS+TY HAP++ +T+QPQ+GAQLILHK+
Sbjct: 491  LSSTYVHAPYTVITLQPQHGAQLILHKL 518



 Score =  157 bits (396), Expect = 1e-35
 Identities = 68/113 (60%), Positives = 89/113 (78%)
 Frame = +1

Query: 94  VFAISWALRTFYWVWVKPKRMEKYLREQGINGPPYKLFYGNLKEMVGMMIEARSRPMELS 273
           ++  SWA + F WVW+ P+++EK LREQGI GP YK   GNLK++V    EAR++PMELS
Sbjct: 16  IYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDIVSATQEARAKPMELS 75

Query: 274 HRIIPRLLPFFYHTVQGYGKLNLSWHGPNPRLNIMDPEMIRDILSNKFGHFGK 432
           H+I+PR++PF + TV+ YGKL+  W+GP  R+NIMDP+MIRDILSNKFGHF K
Sbjct: 76  HKIVPRVMPFVHQTVEKYGKLSFIWYGPYCRVNIMDPDMIRDILSNKFGHFEK 128



 Score = 95.9 bits (237), Expect = 3e-17
 Identities = 41/59 (69%), Positives = 52/59 (88%)
 Frame = +2

Query: 557 EKLKLMVPAFYASCSDLVSKWQKLTSKESCEVDVWPGLQSLTGDAISRTAFGSSYEEGR 733
           EKLK+M+PA Y SCS++V+KW+ + S++SCE+DVWP LQ+LT D ISRTAFGSSYEEGR
Sbjct: 164 EKLKMMLPAIYTSCSEMVNKWEMMVSQDSCELDVWPHLQNLTADVISRTAFGSSYEEGR 222


>ref|XP_002263652.1| PREDICTED: secologanin synthase-like [Vitis vinifera]
          Length = 516

 Score =  341 bits (874), Expect = 5e-91
 Identities = 164/268 (61%), Positives = 202/268 (75%)
 Frame = +3

Query: 873  PTKRNNRMKEIYREVKTLLGDMIKKREKAMKLGEPSTDDLLGLLMESNHKEIKDGGNAKN 1052
            PTKRN RMK+I +E+ TLL D+I KR+KAMK G+ + +DLLG+LMESN KEI++GGN+KN
Sbjct: 249  PTKRNKRMKKIDKEMNTLLNDIITKRDKAMKDGKTANEDLLGILMESNSKEIQEGGNSKN 308

Query: 1053 AGMSIDEVIEECKLFYIAGQETTSTLLLWTMVVLCMHPEWQEKAREEVLQVFGQNKPDLE 1232
            AG+S+ EVIEECKLFY+AGQETTS LLLWTMV+L  HP WQ  AREEV QVFG+NKP+  
Sbjct: 309  AGISMQEVIEECKLFYLAGQETTSNLLLWTMVLLSKHPNWQTLAREEVFQVFGKNKPEFA 368

Query: 1233 GLNHLKIVTMILYEVLRLYPPVVMYSRATYKAIKLGEICIXXXXXXXXXXXXXHHDRDLW 1412
            GL+ LK+VTMI YEVLRLYPP    +RA Y+ I LGE+ +             HHD ++W
Sbjct: 369  GLSRLKVVTMIFYEVLRLYPPGATLNRAVYEDINLGELYLPSGVEIVLPTILVHHDPEIW 428

Query: 1413 GEDAEEFKPERFVEGISKATKNRVSFFPFSYGPRICIGQSFXXXXXXXXXXXXLQNFSFQ 1592
            G+D +EFKPERF EG+ KATK +VS+FPF +GPRICIGQ+F            LQ F+F+
Sbjct: 429  GDDVKEFKPERFSEGVMKATKGQVSYFPFGWGPRICIGQNFAMAEAKMALAMILQCFTFE 488

Query: 1593 LSATYAHAPFSFLTIQPQYGAQLILHKV 1676
            LS +Y HAP S LT+QPQYGA LILHK+
Sbjct: 489  LSPSYTHAPTSVLTLQPQYGAHLILHKI 516



 Score =  110 bits (276), Expect = 1e-21
 Identities = 49/100 (49%), Positives = 73/100 (73%)
 Frame = +1

Query: 103 ISWALRTFYWVWVKPKRMEKYLREQGINGPPYKLFYGNLKEMVGMMIEARSRPMELSHRI 282
           +S A R FY VW++P+R+E+ LR QG+ G  Y+  +G+ K++  M+ EA SRP+ LS  I
Sbjct: 17  LSLAWRAFYLVWLRPRRLERCLRRQGLMGNSYRPLHGDAKKVSIMLKEANSRPINLSDDI 76

Query: 283 IPRLLPFFYHTVQGYGKLNLSWHGPNPRLNIMDPEMIRDI 402
           +PR++PF Y T+Q YGK + +W GP PR+NIM PE+IR++
Sbjct: 77  VPRVIPFLYKTIQQYGKNSFTWVGPIPRVNIMKPELIREV 116



 Score = 84.3 bits (207), Expect = 1e-13
 Identities = 40/60 (66%), Positives = 50/60 (83%), Gaps = 1/60 (1%)
 Frame = +2

Query: 557 EKLKLMVPAFYASCSDLVSKWQKLTSKE-SCEVDVWPGLQSLTGDAISRTAFGSSYEEGR 733
           EKLKLM PAF+ SC  ++SK +++ S E SCE+DVWP L++LT DA+SRTAFGSSYEEGR
Sbjct: 161 EKLKLMSPAFHLSCRQMISKMEEMVSPEGSCELDVWPFLKNLTADALSRTAFGSSYEEGR 220


>gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago truncatula]
          Length = 518

 Score =  335 bits (860), Expect = 2e-89
 Identities = 161/268 (60%), Positives = 199/268 (74%)
 Frame = +3

Query: 873  PTKRNNRMKEIYREVKTLLGDMIKKREKAMKLGEPSTDDLLGLLMESNHKEIKDGGNAKN 1052
            PT    RMKEI R++K  L DMI  RE+A+K GE + DDLLG+L+ESNHKE+++ GN K+
Sbjct: 249  PTATPRRMKEIDRDIKASLTDMINNRERALKAGEATKDDLLGILLESNHKEMEEHGNNKD 308

Query: 1053 AGMSIDEVIEECKLFYIAGQETTSTLLLWTMVVLCMHPEWQEKAREEVLQVFGQNKPDLE 1232
             GMS+D+VIEECKLFY AGQETTS LL+WTMV+L  +P+WQ +AREEVLQVFG NKPD +
Sbjct: 309  VGMSLDDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVLQVFGNNKPDFD 368

Query: 1233 GLNHLKIVTMILYEVLRLYPPVVMYSRATYKAIKLGEICIXXXXXXXXXXXXXHHDRDLW 1412
            GL+HLKIVTMILYEVLRLYPPV+  +R  +K +KLG + +             HHD +LW
Sbjct: 369  GLSHLKIVTMILYEVLRLYPPVIALARRVHKDVKLGNLALPAGVQVFLSIIMVHHDIELW 428

Query: 1413 GEDAEEFKPERFVEGISKATKNRVSFFPFSYGPRICIGQSFXXXXXXXXXXXXLQNFSFQ 1592
            GEDA+ F PERF EG+ KAT  R SFFPF+ GPRICIGQ+F            LQ FSF+
Sbjct: 429  GEDAKVFNPERFSEGVLKATNGRNSFFPFAGGPRICIGQNFSMLEAKMAIAMILQRFSFE 488

Query: 1593 LSATYAHAPFSFLTIQPQYGAQLILHKV 1676
            LS +YAHAP + +T+QPQYGA +ILHK+
Sbjct: 489  LSPSYAHAPATVITLQPQYGAHIILHKL 516



 Score =  110 bits (275), Expect = 1e-21
 Identities = 50/111 (45%), Positives = 74/111 (66%)
 Frame = +1

Query: 100 AISWALRTFYWVWVKPKRMEKYLREQGINGPPYKLFYGNLKEMVGMMIEARSRPMELSHR 279
           ++ WA R   W+W+KPK++EK LREQG+ G  Y+L  G++ +++ M  EA S+PM LS  
Sbjct: 16  SLIWAWRILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDMNDLLKMRKEATSKPMNLSDD 75

Query: 280 IIPRLLPFFYHTVQGYGKLNLSWHGPNPRLNIMDPEMIRDILSNKFGHFGK 432
           I+PR+  F + +V  +GK +  W GP PR+NI DP+ I+D+L NK   F K
Sbjct: 76  IVPRVYSFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVL-NKIYDFQK 125



 Score = 85.5 bits (210), Expect = 4e-14
 Identities = 38/60 (63%), Positives = 48/60 (80%), Gaps = 1/60 (1%)
 Frame = +2

Query: 557 EKLKLMVPAFYASCSDLVSKWQKLTSKE-SCEVDVWPGLQSLTGDAISRTAFGSSYEEGR 733
           EKLK+M+P F+ SC DL+SKW+K+ S + SCE+DVWP LQ+   D ISR AFGSSYEEG+
Sbjct: 161 EKLKIMLPIFHKSCDDLISKWEKMLSSDGSCEMDVWPFLQNFASDVISRAAFGSSYEEGK 220


>dbj|BAL45198.1| cytochrome P450 monooxygenase [Medicago truncatula]
          Length = 518

 Score =  335 bits (860), Expect = 2e-89
 Identities = 161/268 (60%), Positives = 199/268 (74%)
 Frame = +3

Query: 873  PTKRNNRMKEIYREVKTLLGDMIKKREKAMKLGEPSTDDLLGLLMESNHKEIKDGGNAKN 1052
            PT    RMKEI R++K  L DMI  RE+A+K GE + DDLLG+L+ESNHKE+++ GN K+
Sbjct: 249  PTATPRRMKEIDRDIKASLTDMINNRERALKAGEATKDDLLGILLESNHKEMEEHGNNKD 308

Query: 1053 AGMSIDEVIEECKLFYIAGQETTSTLLLWTMVVLCMHPEWQEKAREEVLQVFGQNKPDLE 1232
             GMS+D+VIEECKLFY AGQETTS LL+WTMV+L  +P+WQ +AREEVLQVFG NKPD +
Sbjct: 309  VGMSLDDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVLQVFGNNKPDFD 368

Query: 1233 GLNHLKIVTMILYEVLRLYPPVVMYSRATYKAIKLGEICIXXXXXXXXXXXXXHHDRDLW 1412
            GL+HLKIVTMILYEVLRLYPPV+  +R  +K +KLG + +             HHD +LW
Sbjct: 369  GLSHLKIVTMILYEVLRLYPPVIALARRVHKDVKLGNLALPAGVQVFLSIIMVHHDIELW 428

Query: 1413 GEDAEEFKPERFVEGISKATKNRVSFFPFSYGPRICIGQSFXXXXXXXXXXXXLQNFSFQ 1592
            GEDA+ F PERF EG+ KAT  R SFFPF+ GPRICIGQ+F            LQ FSF+
Sbjct: 429  GEDAKVFNPERFSEGVLKATNGRNSFFPFAGGPRICIGQNFSMLEAKMAIAMILQRFSFE 488

Query: 1593 LSATYAHAPFSFLTIQPQYGAQLILHKV 1676
            LS +YAHAP + +T+QPQYGA +ILHK+
Sbjct: 489  LSPSYAHAPATVITLQPQYGAHIILHKL 516



 Score =  109 bits (273), Expect = 2e-21
 Identities = 49/111 (44%), Positives = 75/111 (67%)
 Frame = +1

Query: 100 AISWALRTFYWVWVKPKRMEKYLREQGINGPPYKLFYGNLKEMVGMMIEARSRPMELSHR 279
           ++ WA R   W+W+KPK++EK LREQG+ G  Y+L  G++K+++ +  EA ++PM LS  
Sbjct: 16  SLIWAWRILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDIKDLLKIRKEATTKPMNLSDD 75

Query: 280 IIPRLLPFFYHTVQGYGKLNLSWHGPNPRLNIMDPEMIRDILSNKFGHFGK 432
           I+PR+  F + +V  +GK +  W GP PR+NI DP+ I+D+L NK   F K
Sbjct: 76  IVPRVYSFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVL-NKIYDFQK 125



 Score = 84.7 bits (208), Expect = 8e-14
 Identities = 37/60 (61%), Positives = 48/60 (80%), Gaps = 1/60 (1%)
 Frame = +2

Query: 557 EKLKLMVPAFYASCSDLVSKWQKLTSKE-SCEVDVWPGLQSLTGDAISRTAFGSSYEEGR 733
           EKLK+M+P F+ SC D++SKW+K+ S + SCE+DVWP LQ+   D ISR AFGSSYEEG+
Sbjct: 161 EKLKIMLPIFHKSCDDMISKWEKMLSSDGSCEMDVWPFLQNFASDVISRAAFGSSYEEGK 220


>emb|CBI39912.3| unnamed protein product [Vitis vinifera]
          Length = 440

 Score =  335 bits (860), Expect = 2e-89
 Identities = 162/265 (61%), Positives = 196/265 (73%)
 Frame = +3

Query: 873  PTKRNNRMKEIYREVKTLLGDMIKKREKAMKLGEPSTDDLLGLLMESNHKEIKDGGNAKN 1052
            PTK N RMK+I +EV  LL  +I KREKAMK GE +  DLLG+LMESN +EI++  N K 
Sbjct: 171  PTKTNRRMKQISKEVYALLRGIINKREKAMKAGETANSDLLGILMESNFREIQEHQNNKK 230

Query: 1053 AGMSIDEVIEECKLFYIAGQETTSTLLLWTMVVLCMHPEWQEKAREEVLQVFGQNKPDLE 1232
              MS+ +VIEECKLFY+AGQETTS LL+WTMV+L  HP WQ +AREEVLQVFG  KP+  
Sbjct: 231  IRMSVKDVIEECKLFYLAGQETTSVLLVWTMVLLSEHPNWQARAREEVLQVFGNKKPEAA 290

Query: 1233 GLNHLKIVTMILYEVLRLYPPVVMYSRATYKAIKLGEICIXXXXXXXXXXXXXHHDRDLW 1412
            GLNHLKIVTMI +EVLRLYPPV M +RA YK  ++G++C              HHD ++W
Sbjct: 291  GLNHLKIVTMIFHEVLRLYPPVAMLARAVYKDTQVGDMCFPAGVQVVLPTILVHHDHEIW 350

Query: 1413 GEDAEEFKPERFVEGISKATKNRVSFFPFSYGPRICIGQSFXXXXXXXXXXXXLQNFSFQ 1592
            G+DA+EF PERF EG+ KATKN+VSFFPF +GPR+CIGQ+F            LQ+FSF+
Sbjct: 351  GDDAKEFNPERFAEGVLKATKNQVSFFPFGWGPRVCIGQNFAMMEAKIALAMILQHFSFE 410

Query: 1593 LSATYAHAPFSFLTIQPQYGAQLIL 1667
            LS +YAHAPFS LT+QPQYGA LIL
Sbjct: 411  LSPSYAHAPFSILTMQPQYGAHLIL 435



 Score = 92.0 bits (227), Expect = 5e-16
 Identities = 42/59 (71%), Positives = 51/59 (86%)
 Frame = +2

Query: 557 EKLKLMVPAFYASCSDLVSKWQKLTSKESCEVDVWPGLQSLTGDAISRTAFGSSYEEGR 733
           EKLK M+PAF+ SCSD+V+KW+ L+   SCE+DVWP L++LTGD ISRTAFGSSYEEGR
Sbjct: 84  EKLKNMLPAFHLSCSDMVTKWKMLSVGGSCELDVWPYLENLTGDVISRTAFGSSYEEGR 142