BLASTX nr result
ID: Coptis23_contig00003115
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00003115
(1441 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002271820.2| PREDICTED: pullulanase 1, chloroplastic-like... 717 0.0
emb|CBI31395.3| unnamed protein product [Vitis vinifera] 717 0.0
ref|XP_002532780.1| pullulanase, putative [Ricinus communis] gi|... 695 0.0
ref|XP_002315334.1| predicted protein [Populus trichocarpa] gi|2... 695 0.0
ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic-like... 680 0.0
>ref|XP_002271820.2| PREDICTED: pullulanase 1, chloroplastic-like [Vitis vinifera]
Length = 896
Score = 717 bits (1850), Expect = 0.0
Identities = 341/479 (71%), Positives = 398/479 (83%)
Frame = +3
Query: 3 LFYSKAYWVTKSLIAWNVDVGNNASCFLFSSQTADLLVNNNCIQGYDLEIRLEPVKDQLP 182
L YS+AYWV++S+IAWNVDVG+ SC+L+SS+ A L + ++ I G+D+ I+LE LP
Sbjct: 16 LLYSRAYWVSESIIAWNVDVGDG-SCYLYSSKIAALSIGDSGITGHDMTIQLEEDNGGLP 74
Query: 183 ASVVDKFPHIRDYRVFHVPDDVDVKSLLKCQLAVGLVNANGECSDVTCLQLPGILDDLYS 362
+V++KFP ++ Y+ F VP VD KSL+KCQLAV + +G+C++VT LQLPG+LD+L+S
Sbjct: 75 INVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNVTGLQLPGVLDELFS 134
Query: 363 YGGPLGAVFSTDTVSLYL*APTAQAVSACIYGAPSGGDPLETVQLEEVNGVWSFDGPRSW 542
Y GPLGA+FS + V+LYL APTAQ V ACIYG PSGGDPLE V LEE NGVWS GP+SW
Sbjct: 135 YEGPLGAIFSKEAVTLYLWAPTAQVVHACIYGDPSGGDPLEIVCLEEFNGVWSASGPKSW 194
Query: 543 EGCYYQYAVSVYHPSTLKIENCMATDPYARGLSSDGRRTLLINLDCENLKPERWDDLANE 722
EGCYY+Y VSVYHPSTL+IE C A DPYARGLS+D RRTLL+NLD ++LKPE WD+LA+E
Sbjct: 195 EGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADARRTLLVNLDSDDLKPEGWDNLADE 254
Query: 723 KPELSSFSDISIYELHIRDFSANDQTVNPDLRGGYLAFTYQDSAGLLHLKKLSNAGLTHV 902
KP L SFSDISIYELHIRDFSA+D TV+PD RGGYLAFT QDSAG+LHLKKL NAG++H+
Sbjct: 255 KPALLSFSDISIYELHIRDFSASDHTVHPDFRGGYLAFTSQDSAGILHLKKLCNAGISHL 314
Query: 903 HLLPTFQFAGVDDEKQKWKNLDADKLARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGV 1082
HLLPTFQFAGVDDEK KWK +D + L LPPDS EQQAQ+ IQD+DGYNWGYNPVLWGV
Sbjct: 315 HLLPTFQFAGVDDEKDKWKCVDPNILETLPPDSVEQQAQIMTIQDEDGYNWGYNPVLWGV 374
Query: 1083 PKXXXXXXXXXXXXXLEFRKMVQSLNRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGY 1262
PK LEFRKMVQ+LNRIG RVVLDVVYNHLHG+GP D+NSVLDKIVPGY
Sbjct: 375 PKGSYASNPNSPCRTLEFRKMVQALNRIGFRVVLDVVYNHLHGSGPFDENSVLDKIVPGY 434
Query: 1263 YLRRSTDGSIEHCTCVNNTASEHFMVERLILDDLLCWVVEYKVDGFRFDLMGHIMKRTM 1439
YLRR++DG IEH CVNNTASEHFMVERLILDDLLCW V+YKVDGFRFDLMGHIMKRTM
Sbjct: 435 YLRRNSDGCIEHSACVNNTASEHFMVERLILDDLLCWAVDYKVDGFRFDLMGHIMKRTM 493
>emb|CBI31395.3| unnamed protein product [Vitis vinifera]
Length = 956
Score = 717 bits (1850), Expect = 0.0
Identities = 341/479 (71%), Positives = 398/479 (83%)
Frame = +3
Query: 3 LFYSKAYWVTKSLIAWNVDVGNNASCFLFSSQTADLLVNNNCIQGYDLEIRLEPVKDQLP 182
L YS+AYWV++S+IAWNVDVG+ SC+L+SS+ A L + ++ I G+D+ I+LE LP
Sbjct: 76 LLYSRAYWVSESIIAWNVDVGDG-SCYLYSSKIAALSIGDSGITGHDMTIQLEEDNGGLP 134
Query: 183 ASVVDKFPHIRDYRVFHVPDDVDVKSLLKCQLAVGLVNANGECSDVTCLQLPGILDDLYS 362
+V++KFP ++ Y+ F VP VD KSL+KCQLAV + +G+C++VT LQLPG+LD+L+S
Sbjct: 135 INVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNVTGLQLPGVLDELFS 194
Query: 363 YGGPLGAVFSTDTVSLYL*APTAQAVSACIYGAPSGGDPLETVQLEEVNGVWSFDGPRSW 542
Y GPLGA+FS + V+LYL APTAQ V ACIYG PSGGDPLE V LEE NGVWS GP+SW
Sbjct: 195 YEGPLGAIFSKEAVTLYLWAPTAQVVHACIYGDPSGGDPLEIVCLEEFNGVWSASGPKSW 254
Query: 543 EGCYYQYAVSVYHPSTLKIENCMATDPYARGLSSDGRRTLLINLDCENLKPERWDDLANE 722
EGCYY+Y VSVYHPSTL+IE C A DPYARGLS+D RRTLL+NLD ++LKPE WD+LA+E
Sbjct: 255 EGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADARRTLLVNLDSDDLKPEGWDNLADE 314
Query: 723 KPELSSFSDISIYELHIRDFSANDQTVNPDLRGGYLAFTYQDSAGLLHLKKLSNAGLTHV 902
KP L SFSDISIYELHIRDFSA+D TV+PD RGGYLAFT QDSAG+LHLKKL NAG++H+
Sbjct: 315 KPALLSFSDISIYELHIRDFSASDHTVHPDFRGGYLAFTSQDSAGILHLKKLCNAGISHL 374
Query: 903 HLLPTFQFAGVDDEKQKWKNLDADKLARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGV 1082
HLLPTFQFAGVDDEK KWK +D + L LPPDS EQQAQ+ IQD+DGYNWGYNPVLWGV
Sbjct: 375 HLLPTFQFAGVDDEKDKWKCVDPNILETLPPDSVEQQAQIMTIQDEDGYNWGYNPVLWGV 434
Query: 1083 PKXXXXXXXXXXXXXLEFRKMVQSLNRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGY 1262
PK LEFRKMVQ+LNRIG RVVLDVVYNHLHG+GP D+NSVLDKIVPGY
Sbjct: 435 PKGSYASNPNSPCRTLEFRKMVQALNRIGFRVVLDVVYNHLHGSGPFDENSVLDKIVPGY 494
Query: 1263 YLRRSTDGSIEHCTCVNNTASEHFMVERLILDDLLCWVVEYKVDGFRFDLMGHIMKRTM 1439
YLRR++DG IEH CVNNTASEHFMVERLILDDLLCW V+YKVDGFRFDLMGHIMKRTM
Sbjct: 495 YLRRNSDGCIEHSACVNNTASEHFMVERLILDDLLCWAVDYKVDGFRFDLMGHIMKRTM 553
>ref|XP_002532780.1| pullulanase, putative [Ricinus communis] gi|223527468|gb|EEF29599.1|
pullulanase, putative [Ricinus communis]
Length = 964
Score = 695 bits (1794), Expect = 0.0
Identities = 336/479 (70%), Positives = 386/479 (80%)
Frame = +3
Query: 3 LFYSKAYWVTKSLIAWNVDVGNNASCFLFSSQTADLLVNNNCIQGYDLEIRLEPVKDQLP 182
L YS+A+WV+K++IAWNVDVG+N SCFL++S T L V+N IQG+D+E++LE LP
Sbjct: 85 LLYSRAFWVSKTIIAWNVDVGDNGSCFLYASDTGSLSVSNAGIQGHDVEVKLEKYNGGLP 144
Query: 183 ASVVDKFPHIRDYRVFHVPDDVDVKSLLKCQLAVGLVNANGECSDVTCLQLPGILDDLYS 362
+VV KFPHIRDYR F P +D KSLLKCQLAV A+G+C T LQLPGILD+LYS
Sbjct: 145 ENVVVKFPHIRDYRAFKAPPTLDAKSLLKCQLAVASYEADGKCRSATGLQLPGILDELYS 204
Query: 363 YGGPLGAVFSTDTVSLYL*APTAQAVSACIYGAPSGGDPLETVQLEEVNGVWSFDGPRSW 542
Y GPLGA +S + VSL+L APTAQAV IY PLE QL+EVNGVWS GP+ W
Sbjct: 205 YDGPLGAHYSKNAVSLHLWAPTAQAVLVYIYKDSFSKVPLEIHQLKEVNGVWSIKGPKDW 264
Query: 543 EGCYYQYAVSVYHPSTLKIENCMATDPYARGLSSDGRRTLLINLDCENLKPERWDDLANE 722
EGCYY Y VSVYHPSTL+IE C A DPYARGLSSDG+RTLL+NL E+LKPE WD+LA E
Sbjct: 265 EGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDGKRTLLVNLTSESLKPEGWDNLAKE 324
Query: 723 KPELSSFSDISIYELHIRDFSANDQTVNPDLRGGYLAFTYQDSAGLLHLKKLSNAGLTHV 902
KP L SF DIS+YELHIRDFSANDQTV+PD RGGYLAFT+++SAG+LHLKKLSNAG+THV
Sbjct: 325 KPSLLSFCDISLYELHIRDFSANDQTVHPDFRGGYLAFTFENSAGVLHLKKLSNAGITHV 384
Query: 903 HLLPTFQFAGVDDEKQKWKNLDADKLARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGV 1082
HLLPTFQFAGVDD K+ WK +D L L PDS EQQAQ+TA+QD+DGYNWGYNPVLWGV
Sbjct: 385 HLLPTFQFAGVDDVKENWKCVDNKMLETLLPDSIEQQAQITAVQDNDGYNWGYNPVLWGV 444
Query: 1083 PKXXXXXXXXXXXXXLEFRKMVQSLNRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGY 1262
PK ++FRKMVQ+LNRIGLRVVLDVVYNHLHG+GP D+NSVLDKIVPGY
Sbjct: 445 PKGSYASNPNGPSRTIQFRKMVQALNRIGLRVVLDVVYNHLHGSGPFDENSVLDKIVPGY 504
Query: 1263 YLRRSTDGSIEHCTCVNNTASEHFMVERLILDDLLCWVVEYKVDGFRFDLMGHIMKRTM 1439
YLRR+ DG IE+ TCVNNTASEH+MVERLI+DDLL W V YKVDGFRFDLMGH+MK TM
Sbjct: 505 YLRRNIDGIIENSTCVNNTASEHYMVERLIIDDLLNWAVNYKVDGFRFDLMGHLMKSTM 563
>ref|XP_002315334.1| predicted protein [Populus trichocarpa] gi|222864374|gb|EEF01505.1|
predicted protein [Populus trichocarpa]
Length = 893
Score = 695 bits (1794), Expect = 0.0
Identities = 327/479 (68%), Positives = 386/479 (80%)
Frame = +3
Query: 3 LFYSKAYWVTKSLIAWNVDVGNNASCFLFSSQTADLLVNNNCIQGYDLEIRLEPVKDQLP 182
L YS+A+WVT+S+IAWN DV + SC+L++SQTA L V + ++G+D +I+LE +P
Sbjct: 14 LLYSRAFWVTQSIIAWNADVVRDGSCYLYASQTAALSVTDGEVEGHDFKIKLEEDSGGIP 73
Query: 183 ASVVDKFPHIRDYRVFHVPDDVDVKSLLKCQLAVGLVNANGECSDVTCLQLPGILDDLYS 362
+V+ KFPH+RDY+ F VP VD KSL+KCQLAV ++G+CS T LQLPG+LD+L++
Sbjct: 74 QNVIAKFPHVRDYKAFKVPSTVDAKSLVKCQLAVATFGSDGKCSYATGLQLPGVLDELFA 133
Query: 363 YGGPLGAVFSTDTVSLYL*APTAQAVSACIYGAPSGGDPLETVQLEEVNGVWSFDGPRSW 542
Y GPLGA +S D VSLYL APTAQAV AC+Y + DP+E VQL+EVNGVWS +G + W
Sbjct: 134 YDGPLGAHYSEDAVSLYLWAPTAQAVCACVYKNANSRDPVEVVQLKEVNGVWSVEGSKDW 193
Query: 543 EGCYYQYAVSVYHPSTLKIENCMATDPYARGLSSDGRRTLLINLDCENLKPERWDDLANE 722
EGCYY Y VSVYHPSTL +E C A DPYARGLS D +RTL +NLD + LKPE W+ LA+E
Sbjct: 194 EGCYYVYEVSVYHPSTLHVEKCYANDPYARGLSPDSQRTLFVNLDSDTLKPEGWEKLADE 253
Query: 723 KPELSSFSDISIYELHIRDFSANDQTVNPDLRGGYLAFTYQDSAGLLHLKKLSNAGLTHV 902
KP + SFSDISIYELH+RDFSAND TV+PD RGGYLAFT +DSAG+LHLKKLSNAG+THV
Sbjct: 254 KPIILSFSDISIYELHVRDFSANDHTVHPDFRGGYLAFTLEDSAGVLHLKKLSNAGITHV 313
Query: 903 HLLPTFQFAGVDDEKQKWKNLDADKLARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGV 1082
HLLPTFQFAGVDD K+ WK +D+ L +LPPDS EQQAQ+T IQDDDGYNWGYNPVLWGV
Sbjct: 314 HLLPTFQFAGVDDVKENWKCVDSTVLEKLPPDSTEQQAQITLIQDDDGYNWGYNPVLWGV 373
Query: 1083 PKXXXXXXXXXXXXXLEFRKMVQSLNRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGY 1262
PK +EFRKMVQ+LN IGLRVVLDVVYNHLHGNGP D+NSVLDKIVPGY
Sbjct: 374 PKGSYASNPSGSCRTIEFRKMVQALNHIGLRVVLDVVYNHLHGNGPFDENSVLDKIVPGY 433
Query: 1263 YLRRSTDGSIEHCTCVNNTASEHFMVERLILDDLLCWVVEYKVDGFRFDLMGHIMKRTM 1439
YLRR+TDG IEH TCVNNTASEH+MVER+I+DD+L W YKVDGFRFDLMGHIMK TM
Sbjct: 434 YLRRNTDGFIEHSTCVNNTASEHYMVERMIIDDMLNWAGNYKVDGFRFDLMGHIMKSTM 492
>ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic-like [Glycine max]
Length = 951
Score = 680 bits (1754), Expect = 0.0
Identities = 326/479 (68%), Positives = 388/479 (81%)
Frame = +3
Query: 3 LFYSKAYWVTKSLIAWNVDVGNNASCFLFSSQTADLLVNNNCIQGYDLEIRLEPVKDQLP 182
L YS+AYWV +SLIAW+VDVGN SC+L +S+ A L + N IQG DL+I+L+ + LP
Sbjct: 70 LLYSRAYWVAESLIAWDVDVGNGFSCYLLASKNASLTIANCQIQGEDLKIKLQEDRVGLP 129
Query: 183 ASVVDKFPHIRDYRVFHVPDDVDVKSLLKCQLAVGLVNANGECSDVTCLQLPGILDDLYS 362
A+VV+KFPHIR ++VF +P +DVK LLK +LAV + +++GEC + T LQLPG+LDDL+S
Sbjct: 130 ANVVEKFPHIRGHKVFSLPPTLDVKPLLKFRLAVVICDSDGECMNCTGLQLPGVLDDLFS 189
Query: 363 YGGPLGAVFSTDTVSLYL*APTAQAVSACIYGAPSGGDPLETVQLEEVNGVWSFDGPRSW 542
Y GPLGA+FS + VSLYL APTAQAV A IY PSG DP+E V LEE NGVW GP+SW
Sbjct: 190 YSGPLGALFSEEAVSLYLWAPTAQAVHAYIYKDPSGDDPIEIVCLEEENGVWRTKGPKSW 249
Query: 543 EGCYYQYAVSVYHPSTLKIENCMATDPYARGLSSDGRRTLLINLDCENLKPERWDDLANE 722
EGCYY Y V VYHPST++IE C +DPYARGLSSDGRR+ L+NLD LKP+ WD+LAN+
Sbjct: 250 EGCYYVYEVCVYHPSTMRIEKCYTSDPYARGLSSDGRRSFLLNLDSVKLKPDGWDNLANK 309
Query: 723 KPELSSFSDISIYELHIRDFSANDQTVNPDLRGGYLAFTYQDSAGLLHLKKLSNAGLTHV 902
KP + SFSDISIYE+HIRDFSA+D +V P+ RGGYLAFT QDSAG+LHLKKLS+AG+THV
Sbjct: 310 KPTIHSFSDISIYEMHIRDFSASDLSVQPEFRGGYLAFTLQDSAGVLHLKKLSSAGITHV 369
Query: 903 HLLPTFQFAGVDDEKQKWKNLDADKLARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGV 1082
HLLPTFQFAGVDD+K+ W+ +D L LPPDSD+QQA +TAIQ+ DGYNWGYNPVLWGV
Sbjct: 370 HLLPTFQFAGVDDQKEDWRFVDTSILESLPPDSDQQQALITAIQNFDGYNWGYNPVLWGV 429
Query: 1083 PKXXXXXXXXXXXXXLEFRKMVQSLNRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGY 1262
PK +EFRKMV +LN IGLRVVLDVVYNHL G+GP D++SVLDKIVPGY
Sbjct: 430 PKGSYASNPNGPYRTIEFRKMVMALNHIGLRVVLDVVYNHLQGSGPFDEHSVLDKIVPGY 489
Query: 1263 YLRRSTDGSIEHCTCVNNTASEHFMVERLILDDLLCWVVEYKVDGFRFDLMGHIMKRTM 1439
YLRR++DG IEH TC+NNTASEHFMVERLILDDL+ W V YK+DGFRFDLMGHIMK TM
Sbjct: 490 YLRRNSDGLIEHSTCINNTASEHFMVERLILDDLVHWAVNYKIDGFRFDLMGHIMKSTM 548