BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00003035
         (1450 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002523698.1| AMP dependent CoA ligase, putative [Ricinus ...   587   e-165
ref|XP_002329323.1| 4-coumarate-coa ligase [Populus trichocarpa]...   579   e-163
gb|AFD33347.1| acyl-activating enzyme 3 [Cannabis sativa]             573   e-161
ref|XP_002872738.1| hypothetical protein ARALYDRAFT_490166 [Arab...   572   e-161
ref|XP_002305282.1| 4-coumarate-coa ligase [Populus trichocarpa]...   572   e-161

>ref|XP_002523698.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223537002|gb|EEF38638.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 542

 Score =  587 bits (1512), Expect = e-165
 Identities = 284/382 (74%), Positives = 335/382 (87%)
 Frame = -3

Query: 1343 NGKLISFSELIKMAGPVTEFPVSIVKQSDTAALLYSSGTTGTSKGVILTHRNFISASLMA 1164
            N ++ SF  L+++ G  +EFP   VKQ+D A LLYSSGTTG SKGVILTH NFI+AS M 
Sbjct: 161  NSRIKSFDHLVELGGSNSEFPTINVKQTDIATLLYSSGTTGISKGVILTHGNFIAASQMI 220

Query: 1163 TADQEFGGERNNVFLCFLPMFHIFGLSIITYSQLQRGNTVISMGRFDIEMVLRSVEKYKV 984
            T DQE  GE +NVFLCFLPMFH+FGL++I YSQLQ GN V+SMG+FD E+VL++VEKY++
Sbjct: 221  TMDQEIAGELHNVFLCFLPMFHVFGLAVIAYSQLQTGNAVVSMGKFDFELVLKAVEKYRI 280

Query: 983  TNLFVVPPVMIAFAKLSVVKKYDLSSLKQLGSGAAPLGKDIMEECAKNIPGAAVVQGYGL 804
            T+L+VVPPV++A AK S+VKKYDLSSL+ +GSGAAPL K++MEECAK IP AA+ QGYG+
Sbjct: 281  THLWVVPPVILALAKQSLVKKYDLSSLQHVGSGAAPLSKELMEECAKTIPHAAIAQGYGM 340

Query: 803  TESCGIVSLENAYEGSRHSGSTGTLVPGVESKIVSVDTMKALPPTELGEIWIRGPNMMQG 624
            TE+ GIVS+EN   G RHSGS GTL  G+E++I+SVDT+K LPP +LGEIW+RGPNMM+G
Sbjct: 341  TETTGIVSVENPRIGVRHSGSAGTLAAGIEAQIISVDTLKPLPPNQLGEIWVRGPNMMRG 400

Query: 623  YFNNPQATKLTIDKQGWIHTGDLGYFDERGQLFVVDRIKELIKYKGFQVAPAELEGLLLT 444
            YFNNPQATK TIDK+GW+HTGDLGYFDE G+L+VVDRIKELIKYKGFQVAPAELEGLL++
Sbjct: 401  YFNNPQATKQTIDKKGWLHTGDLGYFDEDGKLYVVDRIKELIKYKGFQVAPAELEGLLVS 460

Query: 443  HPEILDAVVIPFPDAEAGEVPIAYVVRSPNSSLNEETVQNFIAKQVAPFKRLRRVTFINT 264
            HPE+LDAVVIPFPDAEAGEVP+AYVVRSPNSSL EE VQ +IA QVAPFKRLRRVTFINT
Sbjct: 461  HPELLDAVVIPFPDAEAGEVPVAYVVRSPNSSLTEEEVQKYIADQVAPFKRLRRVTFINT 520

Query: 263  VPKSASGKILRRELIEKVRSKV 198
            VPKSASGKILRRELIEKV+SK+
Sbjct: 521  VPKSASGKILRRELIEKVKSKL 542


>ref|XP_002329323.1| 4-coumarate-coa ligase [Populus trichocarpa]
            gi|224127626|ref|XP_002329324.1| acyl:coa ligase [Populus
            trichocarpa] gi|222870777|gb|EEF07908.1| 4-coumarate-coa
            ligase [Populus trichocarpa] gi|222870778|gb|EEF07909.1|
            acyl:coa ligase [Populus trichocarpa]
          Length = 543

 Score =  579 bits (1493), Expect = e-163
 Identities = 276/383 (72%), Positives = 337/383 (87%)
 Frame = -3

Query: 1346 NNGKLISFSELIKMAGPVTEFPVSIVKQSDTAALLYSSGTTGTSKGVILTHRNFISASLM 1167
            +  ++ SF  L+++ G  +EFPVS VKQSD A LLYSSGTTG SKGVILTH NFI+ASLM
Sbjct: 161  SGSRIRSFHSLVELGGSNSEFPVSDVKQSDIATLLYSSGTTGVSKGVILTHGNFIAASLM 220

Query: 1166 ATADQEFGGERNNVFLCFLPMFHIFGLSIITYSQLQRGNTVISMGRFDIEMVLRSVEKYK 987
             + DQ   GE +NVFLCFLPMFH+FGL++ITYSQLQ GN V+SMG+F+ EMVLR++EKY+
Sbjct: 221  VSMDQVMAGEIHNVFLCFLPMFHVFGLAVITYSQLQMGNAVVSMGKFEFEMVLRTIEKYR 280

Query: 986  VTNLFVVPPVMIAFAKLSVVKKYDLSSLKQLGSGAAPLGKDIMEECAKNIPGAAVVQGYG 807
            VT+++VVPPV++A +K ++VKKYDLSSL+ +GSGAAPLGKD+M+ECAKN+P   ++QG+G
Sbjct: 281  VTHMWVVPPVILALSKQNLVKKYDLSSLRNIGSGAAPLGKDLMKECAKNLPDTTIIQGFG 340

Query: 806  LTESCGIVSLENAYEGSRHSGSTGTLVPGVESKIVSVDTMKALPPTELGEIWIRGPNMMQ 627
            +TE+CGIVSLE+   G RHSGS G L  G+E++I+SV+T K LPP +LGEIW+RGPNMM+
Sbjct: 341  MTETCGIVSLEDPRIGVRHSGSAGILNAGIEAQIISVETAKPLPPNQLGEIWVRGPNMMR 400

Query: 626  GYFNNPQATKLTIDKQGWIHTGDLGYFDERGQLFVVDRIKELIKYKGFQVAPAELEGLLL 447
            GYFNNPQATK TIDK+GW+HTGDLGYFD+ GQLFVVDRIKELIKYKGFQVAPAELEGLL+
Sbjct: 401  GYFNNPQATKDTIDKKGWVHTGDLGYFDDDGQLFVVDRIKELIKYKGFQVAPAELEGLLV 460

Query: 446  THPEILDAVVIPFPDAEAGEVPIAYVVRSPNSSLNEETVQNFIAKQVAPFKRLRRVTFIN 267
            +HPEILDAVV P+PDAEAGEVP+AYVVRSPNS+L EE VQ FI+ QVAPFKRLR+VTFIN
Sbjct: 461  SHPEILDAVVTPYPDAEAGEVPVAYVVRSPNSALTEEDVQKFISDQVAPFKRLRKVTFIN 520

Query: 266  TVPKSASGKILRRELIEKVRSKV 198
            +VPKSASGKILRREL++KV+SK+
Sbjct: 521  SVPKSASGKILRRELVQKVKSKM 543


>gb|AFD33347.1| acyl-activating enzyme 3 [Cannabis sativa]
          Length = 543

 Score =  573 bits (1476), Expect = e-161
 Identities = 281/386 (72%), Positives = 340/386 (88%), Gaps = 2/386 (0%)
 Frame = -3

Query: 1349 NNNGKLISFSELIKMAGPV-TEFP-VSIVKQSDTAALLYSSGTTGTSKGVILTHRNFISA 1176
            +++ K+++F++L+ + G   +EFP V   KQSDTAALLYSSGTTG SKGV+LTH+NFI++
Sbjct: 158  SSSDKVMTFNDLVNLGGSSGSEFPIVDDFKQSDTAALLYSSGTTGMSKGVVLTHKNFIAS 217

Query: 1175 SLMATADQEFGGERNNVFLCFLPMFHIFGLSIITYSQLQRGNTVISMGRFDIEMVLRSVE 996
            SLM T +Q+  GE +NVFLCFLPMFH+FGL+IITY+QLQRGNTVISM RFD+E +L+ VE
Sbjct: 218  SLMVTMEQDLVGEMDNVFLCFLPMFHVFGLAIITYAQLQRGNTVISMARFDLEKMLKDVE 277

Query: 995  KYKVTNLFVVPPVMIAFAKLSVVKKYDLSSLKQLGSGAAPLGKDIMEECAKNIPGAAVVQ 816
            KYKVT+L+VVPPV++A +K S+VKK++LSS+K +GSGAAPLGKD+MEEC+K +P   V Q
Sbjct: 278  KYKVTHLWVVPPVILALSKNSMVKKFNLSSIKYIGSGAAPLGKDLMEECSKVVPYGIVAQ 337

Query: 815  GYGLTESCGIVSLENAYEGSRHSGSTGTLVPGVESKIVSVDTMKALPPTELGEIWIRGPN 636
            GYG+TE+CGIVS+E+   G R+SGS G L  GVE++IVSVDT+K LPP +LGEIW++GPN
Sbjct: 338  GYGMTETCGIVSMEDIRGGKRNSGSAGMLASGVEAQIVSVDTLKPLPPNQLGEIWVKGPN 397

Query: 635  MMQGYFNNPQATKLTIDKQGWIHTGDLGYFDERGQLFVVDRIKELIKYKGFQVAPAELEG 456
            MMQGYFNNPQATKLTIDK+GW+HTGDLGYFDE G L+VVDRIKELIKYKGFQVAPAELEG
Sbjct: 398  MMQGYFNNPQATKLTIDKKGWVHTGDLGYFDEDGHLYVVDRIKELIKYKGFQVAPAELEG 457

Query: 455  LLLTHPEILDAVVIPFPDAEAGEVPIAYVVRSPNSSLNEETVQNFIAKQVAPFKRLRRVT 276
            LL++HPEILDAVVIPFPDAEAGEVP+AYVVRSPNSSL E  V+ FIA QVA FKRLR+VT
Sbjct: 458  LLVSHPEILDAVVIPFPDAEAGEVPVAYVVRSPNSSLTENDVKKFIAGQVASFKRLRKVT 517

Query: 275  FINTVPKSASGKILRRELIEKVRSKV 198
            FIN+VPKSASGKILRRELI+KVRS +
Sbjct: 518  FINSVPKSASGKILRRELIQKVRSNM 543


>ref|XP_002872738.1| hypothetical protein ARALYDRAFT_490166 [Arabidopsis lyrata subsp.
            lyrata] gi|297318575|gb|EFH48997.1| hypothetical protein
            ARALYDRAFT_490166 [Arabidopsis lyrata subsp. lyrata]
          Length = 544

 Score =  572 bits (1474), Expect = e-161
 Identities = 272/391 (69%), Positives = 344/391 (87%)
 Frame = -3

Query: 1370 SVYFPNNNNNGKLISFSELIKMAGPVTEFPVSIVKQSDTAALLYSSGTTGTSKGVILTHR 1191
            SV  P  +N+ K++SF+++++++ PV+++P   +KQSDTAALLYSSGTTGTSKGV LTH 
Sbjct: 155  SVQIPPGSNS-KILSFNDVMELSEPVSDYPFVEIKQSDTAALLYSSGTTGTSKGVELTHG 213

Query: 1190 NFISASLMATADQEFGGERNNVFLCFLPMFHIFGLSIITYSQLQRGNTVISMGRFDIEMV 1011
            NFI+ASLM T DQ+  GE + VFLCFLPMFH+FGL++ITYSQLQRGN ++SM +F++E+V
Sbjct: 214  NFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLTVITYSQLQRGNALVSMAKFELELV 273

Query: 1010 LRSVEKYKVTNLFVVPPVMIAFAKLSVVKKYDLSSLKQLGSGAAPLGKDIMEECAKNIPG 831
            L+++EKY+VT+L+VVPPV +A +K S+VKK+DLSSLK +GSGAAPLGKD+MEEC +NIP 
Sbjct: 274  LKNIEKYRVTHLWVVPPVFLALSKQSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPN 333

Query: 830  AAVVQGYGLTESCGIVSLENAYEGSRHSGSTGTLVPGVESKIVSVDTMKALPPTELGEIW 651
              ++QGYG+TE+CGIVS+E+   G R+SGS G L PGVE++IVSV+T  + PP + GEIW
Sbjct: 334  VLLMQGYGMTETCGIVSVEDPRLGKRNSGSAGMLAPGVEAQIVSVETGNSQPPNQQGEIW 393

Query: 650  IRGPNMMQGYFNNPQATKLTIDKQGWIHTGDLGYFDERGQLFVVDRIKELIKYKGFQVAP 471
            +RGPNMM+GY NNPQATK TIDK+GW+HTGDLGYF+E G L+VVDR+KELIKYKGFQVAP
Sbjct: 394  VRGPNMMKGYLNNPQATKETIDKKGWVHTGDLGYFNEDGNLYVVDRLKELIKYKGFQVAP 453

Query: 470  AELEGLLLTHPEILDAVVIPFPDAEAGEVPIAYVVRSPNSSLNEETVQNFIAKQVAPFKR 291
            AELEGLL++HPEILDAVVIPFPD EAGEVPIA+VVRSPNSS+ E+ +Q FIAKQVAP+KR
Sbjct: 454  AELEGLLVSHPEILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKR 513

Query: 290  LRRVTFINTVPKSASGKILRRELIEKVRSKV 198
            LRRV+FINTVPKSA+GKILRREL+++VRSK+
Sbjct: 514  LRRVSFINTVPKSAAGKILRRELVQQVRSKM 544


>ref|XP_002305282.1| 4-coumarate-coa ligase [Populus trichocarpa]
            gi|222848246|gb|EEE85793.1| 4-coumarate-coa ligase
            [Populus trichocarpa]
          Length = 543

 Score =  572 bits (1474), Expect = e-161
 Identities = 271/383 (70%), Positives = 335/383 (87%)
 Frame = -3

Query: 1346 NNGKLISFSELIKMAGPVTEFPVSIVKQSDTAALLYSSGTTGTSKGVILTHRNFISASLM 1167
            +  ++ SF  L+ + G  ++FP S VKQSD + LLYSSGTTG SKGVILTH NFI+ASLM
Sbjct: 161  SGSRITSFDSLVGLGGSNSQFPSSNVKQSDISTLLYSSGTTGVSKGVILTHGNFIAASLM 220

Query: 1166 ATADQEFGGERNNVFLCFLPMFHIFGLSIITYSQLQRGNTVISMGRFDIEMVLRSVEKYK 987
             T DQ   GE ++VFLCFLPMFH+FGL++ITYSQLQ GN V+SMG+F+ EMVL+++EK++
Sbjct: 221  VTMDQAMAGEMHDVFLCFLPMFHVFGLAVITYSQLQMGNAVVSMGKFEFEMVLKTIEKHR 280

Query: 986  VTNLFVVPPVMIAFAKLSVVKKYDLSSLKQLGSGAAPLGKDIMEECAKNIPGAAVVQGYG 807
            VT+++VVPPV++A AK  +VKKYD+SSL+ +GSGAAPLGKD+M+ECAKN+P   + QGYG
Sbjct: 281  VTDMWVVPPVVLALAKQDMVKKYDISSLRNIGSGAAPLGKDLMKECAKNLPDTIISQGYG 340

Query: 806  LTESCGIVSLENAYEGSRHSGSTGTLVPGVESKIVSVDTMKALPPTELGEIWIRGPNMMQ 627
            +TE+CGIVS+E++  G RHSGS G L  G+E++IVSV+T+K LPP +LGEIW+RGPNMM+
Sbjct: 341  MTETCGIVSVEDSRLGVRHSGSAGILAAGIEAQIVSVETLKPLPPNQLGEIWVRGPNMMR 400

Query: 626  GYFNNPQATKLTIDKQGWIHTGDLGYFDERGQLFVVDRIKELIKYKGFQVAPAELEGLLL 447
            GYFNNPQATK T+DK+GW+HTGD+GYFD+ GQLFVVDRIKELIKYKGFQVAPAELEGLL+
Sbjct: 401  GYFNNPQATKDTMDKKGWVHTGDVGYFDDDGQLFVVDRIKELIKYKGFQVAPAELEGLLV 460

Query: 446  THPEILDAVVIPFPDAEAGEVPIAYVVRSPNSSLNEETVQNFIAKQVAPFKRLRRVTFIN 267
            +HP+ILDAVVIP+PDAEAGEVP+AYVVRSPNS+L E  VQ FIA QVAPFKRLR+VTFIN
Sbjct: 461  SHPQILDAVVIPYPDAEAGEVPVAYVVRSPNSALTEVDVQKFIADQVAPFKRLRKVTFIN 520

Query: 266  TVPKSASGKILRRELIEKVRSKV 198
            TVPKSASGKILRRELI++V+SK+
Sbjct: 521  TVPKSASGKILRRELIQQVKSKI 543