BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00003024
         (2360 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002321248.1| cytochrome P450 probable ent-kaurenoic acid ...   642   0.0  
dbj|BAG71199.1| ent-kaurenoic acid oxidase [Lactuca sativa]           630   e-178
ref|XP_002892270.1| ent-kaurenoic acid hydroxylase 1 [Arabidopsi...   629   e-177
ref|XP_002526524.1| Ent-kaurenoic acid oxidase, putative [Ricinu...   627   e-177
gb|AGF25266.1| ent-kaurenoic acid oxidase [Pyrus communis]            626   e-177

>ref|XP_002321248.1| cytochrome P450 probable ent-kaurenoic acid oxidase [Populus
            trichocarpa] gi|222862021|gb|EEE99563.1| cytochrome P450
            probable ent-kaurenoic acid oxidase [Populus trichocarpa]
          Length = 493

 Score =  642 bits (1655), Expect = 0.0
 Identities = 303/432 (70%), Positives = 369/432 (85%), Gaps = 1/432 (0%)
 Frame = -2

Query: 2359 AFKSNDPESFINGFVKRFGRTGMYKAFMFGNPTIMVTIPETCRQVLMDDEKFKPGWPQST 2180
            AFKS+DP+SF+  F+ ++G  G+YKAFMFGNP++ VT PE CR+VL +D+ FKPGWP ST
Sbjct: 62   AFKSSDPDSFMRTFINKYGDNGIYKAFMFGNPSVFVTTPEACRRVLSNDDAFKPGWPIST 121

Query: 2179 VELIGRKSFVGLSYEDHKWLRRITAAPVNGHEALSVYLEFIEENVVSTLERWSNMGEIEF 2000
            ++LIGRKSF+ +SYE+HK LRR+T+APVNGHEALSVY+ +IEENV++ LE+W+ MG+IEF
Sbjct: 122  LKLIGRKSFIDISYEEHKRLRRLTSAPVNGHEALSVYIPYIEENVIAMLEKWTTMGKIEF 181

Query: 1999 LTQLRKLTFRIIMHIFISKGGESVMDALEEVYTTLNYGVRAMAINLPGFAYYRALKSRKR 1820
            LTQ+RKLTF+IIM+IF+S   E VM+ALE+ YTTLNYGVRAMAINLPGFAYY+ALK+RKR
Sbjct: 182  LTQVRKLTFKIIMYIFLSSESEVVMEALEKDYTTLNYGVRAMAINLPGFAYYKALKARKR 241

Query: 1819 LVEIFLFVVNERRAVRVRDGPPAKK-DMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGH 1643
            LV IF  +V+ERR +R      AKK DM+D+LL V+DEN RKL DEEIID ++MYLNAGH
Sbjct: 242  LVAIFQSIVDERRNLRKNSARNAKKKDMMDSLLGVEDENGRKLTDEEIIDVILMYLNAGH 301

Query: 1642 ESSGHITMWATVLLQEHPDMFQKAKAEQEQIVKNRPSMQKGLRLKEIRQMDYLNKVIDET 1463
            ESSGHIT WAT+ LQEHP+  QKAK EQEQIVK RP  Q GL LKE+R+MDYL+KVIDET
Sbjct: 302  ESSGHITTWATIFLQEHPEFLQKAKEEQEQIVKRRPPAQNGLSLKEVREMDYLSKVIDET 361

Query: 1462 LRLISFSLVVFREAKKDVSMNGYIIPMGWKVEVWLRNVHRDPEVYPDPNKFDPSRWDGFT 1283
            LRLI+FSL VFREAK D S+NGYIIP GWKV VW R VH DPE+Y +P +F+PSRWD +T
Sbjct: 362  LRLITFSLTVFREAKTDFSINGYIIPKGWKVLVWFRTVHLDPEIYQNPKEFNPSRWDNYT 421

Query: 1282 PKAGTYLPFGAGSRLCPGNELAKLEIAIFMHYFLLNYRVERVNPKCPVMYLPHTRHTDNC 1103
            PKAGT+LPFGAGSRLCPGN+LAKLEI+IF+HYFLL+YR+ER NP+C  M+LPHTR TDNC
Sbjct: 422  PKAGTFLPFGAGSRLCPGNDLAKLEISIFLHYFLLDYRLERENPECRWMFLPHTRPTDNC 481

Query: 1102 LARIRKISPPSM 1067
            +ARI+K+S  S+
Sbjct: 482  VARIKKVSSTSV 493


>dbj|BAG71199.1| ent-kaurenoic acid oxidase [Lactuca sativa]
          Length = 493

 Score =  630 bits (1626), Expect = e-178
 Identities = 295/427 (69%), Positives = 356/427 (83%)
 Frame = -2

Query: 2359 AFKSNDPESFINGFVKRFGRTGMYKAFMFGNPTIMVTIPETCRQVLMDDEKFKPGWPQST 2180
            AFKSNDP++FI+ F++RFG  G+YK+FMFG+P+I+VT+PE CR+VL DDE F+PGWP +T
Sbjct: 66   AFKSNDPDAFISNFLRRFGSRGLYKSFMFGSPSIIVTVPEACRKVLFDDEAFQPGWPTAT 125

Query: 2179 VELIGRKSFVGLSYEDHKWLRRITAAPVNGHEALSVYLEFIEENVVSTLERWSNMGEIEF 2000
             ELIG+KSF+G+SYEDHK LR++TAAPVNGHEALS Y+++IE NVV  LE+WS MG IEF
Sbjct: 126  RELIGKKSFIGISYEDHKRLRKLTAAPVNGHEALSTYMQYIETNVVLALEKWSKMGRIEF 185

Query: 1999 LTQLRKLTFRIIMHIFISKGGESVMDALEEVYTTLNYGVRAMAINLPGFAYYRALKSRKR 1820
            LT LRKLTF+IIMHIF+S   E VMDALE+ YT LNYGVRAMAIN+PGFAYY ALK+RK 
Sbjct: 186  LTYLRKLTFQIIMHIFLSTESEHVMDALEKEYTVLNYGVRAMAINIPGFAYYNALKARKN 245

Query: 1819 LVEIFLFVVNERRAVRVRDGPPAKKDMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHE 1640
            LV I   VV ERR  R  +   +KKDMLD LLD  DE  RKL+DEEIIDTL+MYLNAGHE
Sbjct: 246  LVAILQTVVYERRKKREANEGMSKKDMLDALLDTTDEKGRKLDDEEIIDTLVMYLNAGHE 305

Query: 1639 SSGHITMWATVLLQEHPDMFQKAKAEQEQIVKNRPSMQKGLRLKEIRQMDYLNKVIDETL 1460
            SSGHITMW+T+LLQ HP+ F  AK EQE+IVKN P  Q+GL LKE RQM+YL+KVIDETL
Sbjct: 306  SSGHITMWSTILLQAHPEFFHIAKEEQERIVKNMPPTQEGLTLKEYRQMEYLSKVIDETL 365

Query: 1459 RLISFSLVVFREAKKDVSMNGYIIPMGWKVEVWLRNVHRDPEVYPDPNKFDPSRWDGFTP 1280
            RL++FSL+ FREAKKDV + GY IP GWKV +W R+VH +PE+YP P +F+PSRWD   P
Sbjct: 366  RLVTFSLMTFREAKKDVDIKGYFIPKGWKVLLWFRSVHHNPELYPQPKEFNPSRWDDLVP 425

Query: 1279 KAGTYLPFGAGSRLCPGNELAKLEIAIFMHYFLLNYRVERVNPKCPVMYLPHTRHTDNCL 1100
            K GT+LPFGAGSRLCPGN+LAKLEIAIF+H+FL+NY  ER NP+CP+MYLPH+R  DNC+
Sbjct: 426  KPGTFLPFGAGSRLCPGNDLAKLEIAIFLHHFLVNYEFERENPECPIMYLPHSRPKDNCV 485

Query: 1099 ARIRKIS 1079
             R+R++S
Sbjct: 486  GRVRRVS 492


>ref|XP_002892270.1| ent-kaurenoic acid hydroxylase 1 [Arabidopsis lyrata subsp. lyrata]
            gi|297338112|gb|EFH68529.1| ent-kaurenoic acid
            hydroxylase 1 [Arabidopsis lyrata subsp. lyrata]
          Length = 491

 Score =  629 bits (1622), Expect = e-177
 Identities = 298/425 (70%), Positives = 358/425 (84%), Gaps = 2/425 (0%)
 Frame = -2

Query: 2359 AFKSNDPESFINGFVKRFGRTGMYKAFMFGNPTIMVTIPETCRQVLMDDEKFKPGWPQST 2180
            +FK++DP+SF +  +KR+G  G+YKA MFGNP+I+VT P+TCR+VL DD+ FKPGWP ST
Sbjct: 64   SFKTSDPDSFTSTLIKRYGPKGIYKAHMFGNPSIIVTTPDTCRRVLTDDDAFKPGWPTST 123

Query: 2179 VELIGRKSFVGLSYEDHKWLRRITAAPVNGHEALSVYLEFIEENVVSTLERWSNMGEIEF 2000
            +ELIGRKSF+G+S+E+HK LRR+TAAPVNGHEALS Y+ +IEENV++ L++W+ MGE EF
Sbjct: 124  MELIGRKSFIGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEF 183

Query: 1999 LTQLRKLTFRIIMHIFISKGGESVMDALEEVYTTLNYGVRAMAINLPGFAYYRALKSRKR 1820
            LT LRKLTFRIIM+IF+S   E+VMDALE  YT LNYGVRAMA+N+PGFAY+RALK+RK 
Sbjct: 184  LTHLRKLTFRIIMYIFLSSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKT 243

Query: 1819 LVEIFLFVVNERRAVRVRDGPPAKKDMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHE 1640
            LV  F  +V ERR  R ++  P KKDMLDNLL+VKDE+ R L+DEEIID L+MYLNAGHE
Sbjct: 244  LVAAFQSIVTERRNQREQNILPNKKDMLDNLLNVKDEDGRTLDDEEIIDVLLMYLNAGHE 303

Query: 1639 SSGHITMWATVLLQEHPDMFQKAKA-EQEQIVKNRPSMQKGLRLKEIRQMDYLNKVIDET 1463
            SSGH  MWAT+ LQEHP+  Q+AK  EQE I+KNRP  QKGL LKE RQM++L++V+DET
Sbjct: 304  SSGHTIMWATIFLQEHPEFLQRAKVNEQEMILKNRPEGQKGLTLKETRQMEFLSQVVDET 363

Query: 1462 LRLISFSLVVFREAKKDVSMNGYIIPMGWKVEVWLRNVHRDPEVYPDPNKFDPSRWD-GF 1286
            LR+I+FSL  FREAK DV MNGY+IP GWKV  W R+VH DPEVYPDP KFDPSRWD GF
Sbjct: 364  LRVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVYPDPRKFDPSRWDNGF 423

Query: 1285 TPKAGTYLPFGAGSRLCPGNELAKLEIAIFMHYFLLNYRVERVNPKCPVMYLPHTRHTDN 1106
             PKAG +LPFGAGS LCPGN+LAKLEI+IF+H+FLL Y+V+R NPKCPVMYLPHTR TDN
Sbjct: 424  VPKAGAFLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPKCPVMYLPHTRPTDN 483

Query: 1105 CLARI 1091
            CLARI
Sbjct: 484  CLARI 488


>ref|XP_002526524.1| Ent-kaurenoic acid oxidase, putative [Ricinus communis]
            gi|223534199|gb|EEF35915.1| Ent-kaurenoic acid oxidase,
            putative [Ricinus communis]
          Length = 492

 Score =  627 bits (1618), Expect = e-177
 Identities = 296/428 (69%), Positives = 360/428 (84%), Gaps = 2/428 (0%)
 Frame = -2

Query: 2359 AFKSNDPESFINGFVKRFGRTGMYKAFMFGNPTIMVTIPETCRQVLMDDEKFKPGWPQST 2180
            AFKSNDP+SF+  F  R+G  G+YKAFMFG P+++VT  E C++VL DD+ FKPGWP ST
Sbjct: 62   AFKSNDPDSFMRNFTARYGSGGIYKAFMFGKPSVLVTTSEACKRVLTDDDAFKPGWPSST 121

Query: 2179 VELIGRKSFVGLSYEDHKWLRRITAAPVNGHEALSVYLEFIEENVVSTLERWSNMGEIEF 2000
             ELIG+KSF+G+SYE+HK LRR+TA+PVNGHEALSVY+ +IE+ V S LE+WS MGEI+F
Sbjct: 122  TELIGKKSFIGISYEEHKRLRRLTASPVNGHEALSVYMHYIEDKVKSALEKWSTMGEIQF 181

Query: 1999 LTQLRKLTFRIIMHIFISKGGESVMDALEEVYTTLNYGVRAMAINLPGFAYYRALKSRKR 1820
            LTQLRKLTFRIIM+IF+S    SVM+ALE  YTTLNYGVRAMAINLPGFAYY+ALK+RK 
Sbjct: 182  LTQLRKLTFRIIMYIFLSSESHSVMEALEREYTTLNYGVRAMAINLPGFAYYKALKARKN 241

Query: 1819 LVEIFLFVVNERRAVRVRDGPPA--KKDMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAG 1646
            LV +  FVV+ RR  +    P +  KKDM+D LLDV+DE  RKL+DEEI+D L+MYLNAG
Sbjct: 242  LVAVLQFVVDARRNQKKGAEPNSSKKKDMMDALLDVEDEKGRKLSDEEIVDVLLMYLNAG 301

Query: 1645 HESSGHITMWATVLLQEHPDMFQKAKAEQEQIVKNRPSMQKGLRLKEIRQMDYLNKVIDE 1466
            HESSGHITMWATV LQEHP+  QKA+ EQE+I+K RP  QKGL LKE+R M+YL+KVIDE
Sbjct: 302  HESSGHITMWATVFLQEHPEFLQKARQEQEEIIKRRPPTQKGLTLKEVRDMEYLSKVIDE 361

Query: 1465 TLRLISFSLVVFREAKKDVSMNGYIIPMGWKVEVWLRNVHRDPEVYPDPNKFDPSRWDGF 1286
            TLRLI+FSLVVFREAK +V+++GY+IP GWK+ VW R+VH DPE+YP+P +F+PSRWD  
Sbjct: 362  TLRLITFSLVVFREAKTNVNISGYVIPKGWKILVWFRSVHLDPEIYPNPREFNPSRWDNH 421

Query: 1285 TPKAGTYLPFGAGSRLCPGNELAKLEIAIFMHYFLLNYRVERVNPKCPVMYLPHTRHTDN 1106
            T KAGT+LPFGAGSR+CPGN+LAKLEIAIF+H+FLLNY +ER+NP   +MYLPH+R  DN
Sbjct: 422  TAKAGTFLPFGAGSRMCPGNDLAKLEIAIFLHHFLLNYELERLNPGSSMMYLPHSRPKDN 481

Query: 1105 CLARIRKI 1082
            CLARI+KI
Sbjct: 482  CLARIKKI 489


>gb|AGF25266.1| ent-kaurenoic acid oxidase [Pyrus communis]
          Length = 503

 Score =  626 bits (1615), Expect = e-177
 Identities = 299/426 (70%), Positives = 365/426 (85%), Gaps = 1/426 (0%)
 Frame = -2

Query: 2359 AFKSNDPESFINGFVKRFGRTGMYKAFMFGNPTIMVTIPETCRQVLMDDEKFKPGWPQST 2180
            AFKSN+PESF++ FV RFG+TG+YKAFMFG P+I+VT PET ++VL DD+ FKPGWP ST
Sbjct: 72   AFKSNNPESFLDKFVSRFGKTGIYKAFMFGFPSIIVTTPETSKRVLTDDDAFKPGWPVST 131

Query: 2179 VELIGRKSFVGLSYEDHKWLRRITAAPVNGHEALSVYLEFIEENVVSTLERWSNMGEIEF 2000
            VELIG+KSF+G+SYE+HK LRR+TAAPVNGHEALSVY+++IEE V+++LE+WS +G+IEF
Sbjct: 132  VELIGKKSFIGISYEEHKRLRRLTAAPVNGHEALSVYMKYIEEIVITSLEKWSKLGQIEF 191

Query: 1999 LTQLRKLTFRIIMHIFISKGGESVMDALEEVYTTLNYGVRAMAINLPGFAYYRALKSRKR 1820
            LTQLRKLTF+IIM+IF+S   E VM+ALE  YT LNYGVRAMAINLPGFAY++ALK+RK 
Sbjct: 192  LTQLRKLTFKIIMYIFLSSESEPVMEALEREYTVLNYGVRAMAINLPGFAYHKALKARKN 251

Query: 1819 LVEIFLFVVNERRAVRVRDGPPAKK-DMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGH 1643
            LV IF  +V+ERRA R       KK DM+D LLDV+D++ RKLNDE+IID L+MYLNAGH
Sbjct: 252  LVAIFQSIVDERRAQRKSGNYYVKKKDMMDALLDVEDDDGRKLNDEDIIDVLLMYLNAGH 311

Query: 1642 ESSGHITMWATVLLQEHPDMFQKAKAEQEQIVKNRPSMQKGLRLKEIRQMDYLNKVIDET 1463
            ESSGH  MWATV LQ +P+ FQ+AKAEQE+I+K RP  QKGL LKE R+MDYL +VIDET
Sbjct: 312  ESSGHTIMWATVFLQANPEAFQRAKAEQEEILKRRPPTQKGLTLKEYREMDYLCQVIDET 371

Query: 1462 LRLISFSLVVFREAKKDVSMNGYIIPMGWKVEVWLRNVHRDPEVYPDPNKFDPSRWDGFT 1283
            LR+I+FSL VFREAKKDV +NGY IP GWKV VW R++H D E+YP+P +F+PSRWD FT
Sbjct: 372  LRVITFSLTVFREAKKDVKINGYSIPKGWKVLVWFRSIHYDSELYPNPMEFNPSRWDNFT 431

Query: 1282 PKAGTYLPFGAGSRLCPGNELAKLEIAIFMHYFLLNYRVERVNPKCPVMYLPHTRHTDNC 1103
            PKA ++LPFGAGS LCPGN+LAKLEIAIF+H+FLLNY++ER NP CP+MYLPHTR  DNC
Sbjct: 432  PKAFSFLPFGAGSHLCPGNDLAKLEIAIFLHHFLLNYKMERTNPGCPLMYLPHTRPKDNC 491

Query: 1102 LARIRK 1085
            +A+I+K
Sbjct: 492  VAKIKK 497