BLASTX nr result
ID: Coptis23_contig00003002
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00003002
(1726 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002275214.1| PREDICTED: short-chain dehydrogenase/reducta... 731 0.0
gb|AFX66971.1| 3-beta-hydroxy-delta5-steroid dehydrogenase [Sola... 726 0.0
ref|XP_002510725.1| hydroxysteroid dehydrogenase, putative [Rici... 724 0.0
emb|CBI40764.3| unnamed protein product [Vitis vinifera] 719 0.0
ref|XP_004145560.1| PREDICTED: short-chain dehydrogenase/reducta... 698 0.0
>ref|XP_002275214.1| PREDICTED: short-chain dehydrogenase/reductase family 42E member 1
[Vitis vinifera]
Length = 478
Score = 731 bits (1887), Expect = 0.0
Identities = 351/477 (73%), Positives = 407/477 (85%)
Frame = +2
Query: 11 MHLSENEGIEKNXXXXXXXXXXXXSALCCELIRRGAHEVRTLDPRASSPWSDHLKNIGRV 190
MHLSENEGIE + +ALC EL+RRGA ++R +D R++SPWSD L+N G V
Sbjct: 1 MHLSENEGIEGSTFVVTGGLGFVGAALCLELVRRGARQIRAIDLRSTSPWSDDLENKG-V 59
Query: 191 RFIQGDVSKKKDVGKALKGADCVFHLASYGMSGKEMMQTGRIDNVNINGTCHVLDACLEF 370
IQGD++ KKDV +AL+GADCVFHLASYGMSGKEM+Q GR+D VNINGTCH+LDAC+EF
Sbjct: 60 HCIQGDITVKKDVERALRGADCVFHLASYGMSGKEMIQYGRVDEVNINGTCHILDACIEF 119
Query: 371 GIKRLVYVSTYNVVFGGKEIINGNEALPYFPMDDHVDPYGRSKSIAEQLVLKSSGRPSKK 550
GIKRLVY STYNVVFGGKEI+NGNEALPYFP+DDHVD Y RSKSIAEQLVLK++GRP K
Sbjct: 120 GIKRLVYTSTYNVVFGGKEILNGNEALPYFPLDDHVDSYSRSKSIAEQLVLKNNGRPFKN 179
Query: 551 KNGTCLYTCAIRPAAIYGPGEERHFPRIVSLAKLGLYTFRIGDKDVKSDWVYVDNLVLAL 730
K+G CLYTCA+RPAAIYGPGE+RHFPRI+SLAKLG+ F IG+ +VK DW+YVDNLV A
Sbjct: 180 KSGKCLYTCAVRPAAIYGPGEDRHFPRIISLAKLGVLPFTIGEANVKGDWIYVDNLVHAQ 239
Query: 731 ILASMGLLDDIPGKKGRPVAAGQPYFISDGSPINTFEFMQPLLRSLEYDLPKATLDVPRA 910
ILASMGLLDDIPG++ RP+AAGQ YFI+DGSP+N +EF++PLLRSLEYDLPKA+L VP A
Sbjct: 240 ILASMGLLDDIPGREKRPIAAGQSYFINDGSPVNIYEFLRPLLRSLEYDLPKASLPVPYA 299
Query: 911 LRLGWIFWYLYSFFYPWLNRSWLPQPLILPAEVYKVGVTHYFSYVKAKEELRYVPMVSPQ 1090
L + I +Y+ YPWLNR WLPQPL+LPAEVYKVGVTHYFSY+KAKEEL YVP+VSP+
Sbjct: 300 LFMSRINCMIYTLLYPWLNRWWLPQPLMLPAEVYKVGVTHYFSYLKAKEELGYVPLVSPR 359
Query: 1091 EGMSRTISYWQDQKRKTLDGPTIYAWLFCIIGMSSLFCAAFAPEYWPVKIMRAISLFFFR 1270
EGM+ TISYWQ++KR++L+GPT+ WLFCIIGM LFCAA+ P+ PV I RAISLFF R
Sbjct: 360 EGMAATISYWQERKRRSLEGPTLQTWLFCIIGMFVLFCAAYLPDIGPVPIFRAISLFFLR 419
Query: 1271 SMTTIRVVFLLATAAHIIEAVYAWNLAKRVDPANAKGWFWQTFVLGGFSLRFLLKRA 1441
SM IRVVFLLATAAHI EAVYAW+LAKRVDPANA+GWFWQTF LG FSLRFLLKRA
Sbjct: 420 SMAIIRVVFLLATAAHIGEAVYAWHLAKRVDPANARGWFWQTFALGIFSLRFLLKRA 476
>gb|AFX66971.1| 3-beta-hydroxy-delta5-steroid dehydrogenase [Solanum tuberosum]
Length = 478
Score = 726 bits (1873), Expect = 0.0
Identities = 343/477 (71%), Positives = 401/477 (84%)
Frame = +2
Query: 11 MHLSENEGIEKNXXXXXXXXXXXXSALCCELIRRGAHEVRTLDPRASSPWSDHLKNIGRV 190
MHLSENEGIEK +ALC EL+RRGA V+ D R SPWS L+ G V
Sbjct: 1 MHLSENEGIEKKSFVVTGGLGFIGAALCLELVRRGARLVKAFDLRTHSPWSSQLRQYG-V 59
Query: 191 RFIQGDVSKKKDVGKALKGADCVFHLASYGMSGKEMMQTGRIDNVNINGTCHVLDACLEF 370
IQGD++ K+ V KAL+G+DCVFHLASYGMSGKEM+Q R+D VNINGTCH++DACL+
Sbjct: 60 HLIQGDITDKQHVQKALQGSDCVFHLASYGMSGKEMLQYSRVDEVNINGTCHIIDACLDH 119
Query: 371 GIKRLVYVSTYNVVFGGKEIINGNEALPYFPMDDHVDPYGRSKSIAEQLVLKSSGRPSKK 550
IKRLVYVST NVV+GGKEI+NGNE LPYFP+DDHVDPYGRSKSIAEQLVLKS+GRP K
Sbjct: 120 QIKRLVYVSTPNVVYGGKEIVNGNENLPYFPIDDHVDPYGRSKSIAEQLVLKSNGRPFTK 179
Query: 551 KNGTCLYTCAIRPAAIYGPGEERHFPRIVSLAKLGLYTFRIGDKDVKSDWVYVDNLVLAL 730
KNG CLYTCAIRPAAIYGPGEERH PRI++L KLGL+ F+IG +VKSDWVYVDNLVLAL
Sbjct: 180 KNGKCLYTCAIRPAAIYGPGEERHLPRIITLTKLGLFPFKIGSPNVKSDWVYVDNLVLAL 239
Query: 731 ILASMGLLDDIPGKKGRPVAAGQPYFISDGSPINTFEFMQPLLRSLEYDLPKATLDVPRA 910
+LASMGLLDDIPG++G P+AAGQPYFISDGSPIN+FEF+ PLL+SL+YDLPK +L V A
Sbjct: 240 LLASMGLLDDIPGREGLPIAAGQPYFISDGSPINSFEFLLPLLKSLDYDLPKTSLAVSHA 299
Query: 911 LRLGWIFWYLYSFFYPWLNRSWLPQPLILPAEVYKVGVTHYFSYVKAKEELRYVPMVSPQ 1090
L LG IFW YSF YPWLN WLPQPLILPAEVYKVGVTHYFS++KAKEEL YVPMVS +
Sbjct: 300 LLLGKIFWAFYSFLYPWLNSRWLPQPLILPAEVYKVGVTHYFSFLKAKEELGYVPMVSSK 359
Query: 1091 EGMSRTISYWQDQKRKTLDGPTIYAWLFCIIGMSSLFCAAFAPEYWPVKIMRAISLFFFR 1270
EGM+ TI+YWQ++KR++LDGPTI+AWLFC+IGMS+LF AA+ P+Y P+ +RA+ LFFFR
Sbjct: 360 EGMAATIAYWQERKRRSLDGPTIWAWLFCVIGMSALFAAAYLPDYGPIPFIRAVHLFFFR 419
Query: 1271 SMTTIRVVFLLATAAHIIEAVYAWNLAKRVDPANAKGWFWQTFVLGGFSLRFLLKRA 1441
SM ++V+F+LA AAH+ EA+YAWN+AK +DPANA+GWFWQTF LG FSLR LLKRA
Sbjct: 420 SMLALKVIFVLAAAAHVGEAIYAWNVAKTIDPANARGWFWQTFALGIFSLRLLLKRA 476
>ref|XP_002510725.1| hydroxysteroid dehydrogenase, putative [Ricinus communis]
gi|223551426|gb|EEF52912.1| hydroxysteroid dehydrogenase,
putative [Ricinus communis]
Length = 478
Score = 724 bits (1869), Expect = 0.0
Identities = 352/477 (73%), Positives = 400/477 (83%)
Frame = +2
Query: 11 MHLSENEGIEKNXXXXXXXXXXXXSALCCELIRRGAHEVRTLDPRASSPWSDHLKNIGRV 190
MHL+ENEGIE N SALC EL+RRGA +VR D R +SPWS L + G V
Sbjct: 1 MHLTENEGIENNNFAVTGGLGFVGSALCLELMRRGARQVRAFDVRPTSPWSHQLTSRG-V 59
Query: 191 RFIQGDVSKKKDVGKALKGADCVFHLASYGMSGKEMMQTGRIDNVNINGTCHVLDACLEF 370
I+GD+++K DV KAL+G DCVFHLAS+GMSGKEM++ GR+D VNINGTCHVL+AC+EF
Sbjct: 60 HCIRGDLTRKSDVEKALRGVDCVFHLASFGMSGKEMIRFGRVDEVNINGTCHVLEACIEF 119
Query: 371 GIKRLVYVSTYNVVFGGKEIINGNEALPYFPMDDHVDPYGRSKSIAEQLVLKSSGRPSKK 550
GIKRLVYVSTYNVVFGGK+I+NGNE+LPYFP DDHVD YG SKSIAEQLVLK +GRP KK
Sbjct: 120 GIKRLVYVSTYNVVFGGKQIVNGNESLPYFPTDDHVDSYGGSKSIAEQLVLKYNGRPLKK 179
Query: 551 KNGTCLYTCAIRPAAIYGPGEERHFPRIVSLAKLGLYTFRIGDKDVKSDWVYVDNLVLAL 730
NG LYTCAIRPAAIYGPGEERH PRIVS AKLGL FRIGD +VK+DWVYVDNLVLAL
Sbjct: 180 NNGKRLYTCAIRPAAIYGPGEERHMPRIVSYAKLGLMPFRIGDANVKTDWVYVDNLVLAL 239
Query: 731 ILASMGLLDDIPGKKGRPVAAGQPYFISDGSPINTFEFMQPLLRSLEYDLPKATLDVPRA 910
ILASMGLLDDIP G PVAAGQPYFISDGSPIN+FEF+QPLL+SL YD+PKA+L VP A
Sbjct: 240 ILASMGLLDDIPNSGGHPVAAGQPYFISDGSPINSFEFLQPLLKSLNYDMPKASLSVPHA 299
Query: 911 LRLGWIFWYLYSFFYPWLNRSWLPQPLILPAEVYKVGVTHYFSYVKAKEELRYVPMVSPQ 1090
L LG IF +Y+ YPWLNR WLPQP ILPAEVYKVGVTHYFS++KAKEEL YVPMV+P+
Sbjct: 300 LILGKIFGAIYTLLYPWLNRWWLPQPFILPAEVYKVGVTHYFSFLKAKEELGYVPMVTPR 359
Query: 1091 EGMSRTISYWQDQKRKTLDGPTIYAWLFCIIGMSSLFCAAFAPEYWPVKIMRAISLFFFR 1270
EGM+ TI+YWQ++KRK+LDGPTIY WLF +IGMS+LFCAA+ P+ PV + RAISLFF R
Sbjct: 360 EGMAATIAYWQEKKRKSLDGPTIYIWLFAVIGMSTLFCAAYLPDIGPVPLFRAISLFFLR 419
Query: 1271 SMTTIRVVFLLATAAHIIEAVYAWNLAKRVDPANAKGWFWQTFVLGGFSLRFLLKRA 1441
SM R VFLLA+AAHI E++YAW+LAKRVDPANA+GWFWQT LG FSLRFLLKRA
Sbjct: 420 SMRMTRTVFLLASAAHIGESIYAWHLAKRVDPANARGWFWQTLALGIFSLRFLLKRA 476
>emb|CBI40764.3| unnamed protein product [Vitis vinifera]
Length = 479
Score = 719 bits (1855), Expect = 0.0
Identities = 344/471 (73%), Positives = 401/471 (85%)
Frame = +2
Query: 11 MHLSENEGIEKNXXXXXXXXXXXXSALCCELIRRGAHEVRTLDPRASSPWSDHLKNIGRV 190
MHLSENEGIE + +ALC EL+RRGA ++R +D R++SPWSD L+N G V
Sbjct: 1 MHLSENEGIEGSTFVVTGGLGFVGAALCLELVRRGARQIRAIDLRSTSPWSDDLENKG-V 59
Query: 191 RFIQGDVSKKKDVGKALKGADCVFHLASYGMSGKEMMQTGRIDNVNINGTCHVLDACLEF 370
IQGD++ KKDV +AL+GADCVFHLASYGMSGKEM+Q GR+D VNINGTCH+LDAC+EF
Sbjct: 60 HCIQGDITVKKDVERALRGADCVFHLASYGMSGKEMIQYGRVDEVNINGTCHILDACIEF 119
Query: 371 GIKRLVYVSTYNVVFGGKEIINGNEALPYFPMDDHVDPYGRSKSIAEQLVLKSSGRPSKK 550
GIKRLVY STYNVVFGGKEI+NGNEALPYFP+DDHVD Y RSKSIAEQLVLK++GRP K
Sbjct: 120 GIKRLVYTSTYNVVFGGKEILNGNEALPYFPLDDHVDSYSRSKSIAEQLVLKNNGRPFKN 179
Query: 551 KNGTCLYTCAIRPAAIYGPGEERHFPRIVSLAKLGLYTFRIGDKDVKSDWVYVDNLVLAL 730
K+G CLYTCA+RPAAIYGPGE+RHFPRI+SLAKLG+ F IG+ +VK DW+YVDNLV A
Sbjct: 180 KSGKCLYTCAVRPAAIYGPGEDRHFPRIISLAKLGVLPFTIGEANVKGDWIYVDNLVHAQ 239
Query: 731 ILASMGLLDDIPGKKGRPVAAGQPYFISDGSPINTFEFMQPLLRSLEYDLPKATLDVPRA 910
ILASMGLLDDIPG++ RP+AAGQ YFI+DGSP+N +EF++PLLRSLEYDLPKA+L VP A
Sbjct: 240 ILASMGLLDDIPGREKRPIAAGQSYFINDGSPVNIYEFLRPLLRSLEYDLPKASLPVPYA 299
Query: 911 LRLGWIFWYLYSFFYPWLNRSWLPQPLILPAEVYKVGVTHYFSYVKAKEELRYVPMVSPQ 1090
L + I +Y+ YPWLNR WLPQPL+LPAEVYKVGVTHYFSY+KAKEEL YVP+VSP+
Sbjct: 300 LFMSRINCMIYTLLYPWLNRWWLPQPLMLPAEVYKVGVTHYFSYLKAKEELGYVPLVSPR 359
Query: 1091 EGMSRTISYWQDQKRKTLDGPTIYAWLFCIIGMSSLFCAAFAPEYWPVKIMRAISLFFFR 1270
EGM+ TISYWQ++KR++L+GPT+ WLFCIIGM LFCAA+ P+ PV I RAISLFF R
Sbjct: 360 EGMAATISYWQERKRRSLEGPTLQTWLFCIIGMFVLFCAAYLPDIGPVPIFRAISLFFLR 419
Query: 1271 SMTTIRVVFLLATAAHIIEAVYAWNLAKRVDPANAKGWFWQTFVLGGFSLR 1423
SM IRVVFLLATAAHI EAVYAW+LAKRVDPANA+GWFWQTF LG FSL+
Sbjct: 420 SMAIIRVVFLLATAAHIGEAVYAWHLAKRVDPANARGWFWQTFALGIFSLQ 470
>ref|XP_004145560.1| PREDICTED: short-chain dehydrogenase/reductase family 42E member
1-like [Cucumis sativus] gi|449522962|ref|XP_004168494.1|
PREDICTED: short-chain dehydrogenase/reductase family 42E
member 1-like [Cucumis sativus]
Length = 478
Score = 698 bits (1802), Expect = 0.0
Identities = 331/477 (69%), Positives = 394/477 (82%)
Frame = +2
Query: 11 MHLSENEGIEKNXXXXXXXXXXXXSALCCELIRRGAHEVRTLDPRASSPWSDHLKNIGRV 190
MHLS +EGIE N SALC EL+RRGA +VR D R +SPWSDHLK G V
Sbjct: 1 MHLSASEGIEGNTFTVTGGLGFAGSALCLELLRRGALQVRAFDLRPASPWSDHLKTQG-V 59
Query: 191 RFIQGDVSKKKDVGKALKGADCVFHLASYGMSGKEMMQTGRIDNVNINGTCHVLDACLEF 370
+ IQGDV+ KKDV +AL+G DCVFHLA+YG+SGKEM+Q GRID VNINGTCHVLDACLEF
Sbjct: 60 KIIQGDVTCKKDVERALRGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLDACLEF 119
Query: 371 GIKRLVYVSTYNVVFGGKEIINGNEALPYFPMDDHVDPYGRSKSIAEQLVLKSSGRPSKK 550
G++RL+Y+STYNVVFG +EI+NGNE LPY P+DDHVD YGRSKSIAEQLVLK++GRP K
Sbjct: 120 GVRRLIYMSTYNVVFGSQEIVNGNEGLPYLPIDDHVDAYGRSKSIAEQLVLKTNGRPLKN 179
Query: 551 KNGTCLYTCAIRPAAIYGPGEERHFPRIVSLAKLGLYTFRIGDKDVKSDWVYVDNLVLAL 730
+NG CL+TCAIR AIYGPGEERHF R+VSLAKLGL FR+G + K+DW+YVDNLVLAL
Sbjct: 180 RNGKCLHTCAIRSCAIYGPGEERHFTRLVSLAKLGLLPFRVGKQSAKTDWIYVDNLVLAL 239
Query: 731 ILASMGLLDDIPGKKGRPVAAGQPYFISDGSPINTFEFMQPLLRSLEYDLPKATLDVPRA 910
ILASMGLLDDIPGK PVAAGQPY++SDG P+N+FEF++PLL SL YDLP L VP+A
Sbjct: 240 ILASMGLLDDIPGKGKDPVAAGQPYYVSDGHPVNSFEFVKPLLNSLGYDLPNYYLPVPKA 299
Query: 911 LRLGWIFWYLYSFFYPWLNRSWLPQPLILPAEVYKVGVTHYFSYVKAKEELRYVPMVSPQ 1090
L LG F LY+ YPWL+R WLP PL+LPAE+YKVGV++YFSY+KAKEEL Y P+V+P+
Sbjct: 300 LPLGKFFALLYTILYPWLDRWWLPHPLMLPAEIYKVGVSNYFSYLKAKEELGYAPIVTPK 359
Query: 1091 EGMSRTISYWQDQKRKTLDGPTIYAWLFCIIGMSSLFCAAFAPEYWPVKIMRAISLFFFR 1270
EGM+ TISYWQ+++RK+LDGPTIY WLFC++GMS LFCAAF P+ PV +AISLFFFR
Sbjct: 360 EGMAATISYWQERERKSLDGPTIYVWLFCLVGMSILFCAAFLPDVGPVPFFKAISLFFFR 419
Query: 1271 SMTTIRVVFLLATAAHIIEAVYAWNLAKRVDPANAKGWFWQTFVLGGFSLRFLLKRA 1441
S+ +R+VFL+A H+ EA+YAW LA++VDPAN++GWFWQTF LG FSLRFLLKRA
Sbjct: 420 SIKVLRMVFLVALLLHVGEAIYAWFLARKVDPANSRGWFWQTFALGFFSLRFLLKRA 476