BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00002989
         (1291 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002513571.1| spermidine synthase 1, putative [Ricinus com...   491   e-144
ref|XP_004145928.1| PREDICTED: spermine synthase-like [Cucumis s...   486   e-144
dbj|BAE19758.1| spermine synthase [Malus sylvestris]                  481   e-143
emb|CAM35498.1| spermine synthase [Lotus japonicus]                   484   e-141
ref|XP_002318386.1| predicted protein [Populus trichocarpa] gi|2...   482   e-139

>ref|XP_002513571.1| spermidine synthase 1, putative [Ricinus communis]
            gi|223547479|gb|EEF48974.1| spermidine synthase 1,
            putative [Ricinus communis]
          Length = 368

 Score =  491 bits (1265), Expect(2) = e-144
 Identities = 239/294 (81%), Positives = 259/294 (88%)
 Frame = +3

Query: 369  SRRSYYNNPMWPGEAHSLQAEKILFKEKSEYQEILVFESVTYGKVLVLDGIVQLTEKDEC 548
            S+R Y+NNPMWPGEAHSL+ + IL+K KSEYQEILVFES  YGKVLVLDGIVQLTEKDEC
Sbjct: 66   SKRVYFNNPMWPGEAHSLEVKNILYKGKSEYQEILVFESSAYGKVLVLDGIVQLTEKDEC 125

Query: 549  AYQEMIVHLPLCSISSPKNXXXXXXXXXXXXREISRHRSVKCIDICEIDKMVIDVSKKFF 728
            AYQEMIVHLPLCSI SPK+            REISRH SV+ IDICEIDKMVIDV K+FF
Sbjct: 126  AYQEMIVHLPLCSIPSPKSVLVVGGGDGGVLREISRHSSVELIDICEIDKMVIDVCKEFF 185

Query: 729  PNLSVGFEDPRVRLHVGDAVEFLRNAPEGMYDAIIVDSSDPVGPAQELVERPFFEMIARA 908
            P L VGFEDPRVRLHVGDAVEFLR AP G YDAIIVDSSDPVGPAQELVE+PFF+ IA+A
Sbjct: 186  PELYVGFEDPRVRLHVGDAVEFLRLAPVGKYDAIIVDSSDPVGPAQELVEKPFFQTIAKA 245

Query: 909  LRPGGVLCNMAESMWLHTHLIQDMISICRQIFKGSVHYAWTNVPTYPSGVIGFLICSTAG 1088
            LRPGGVLCNMAESMWLHTHLIQDMISICR+ FKGSVHYAW +VPTYPSGVIGFLICST G
Sbjct: 246  LRPGGVLCNMAESMWLHTHLIQDMISICRETFKGSVHYAWASVPTYPSGVIGFLICSTEG 305

Query: 1089 PPVDFLNPINPIEKIEGALKFNRELRFYNSEMHVAAFALPSFLRREVSILRDSP 1250
            PPVDFLNP+NPIEK+EGA K+ RELRFYNSE+H AAFALP+FL+REV +LRDSP
Sbjct: 306  PPVDFLNPVNPIEKLEGADKYKRELRFYNSEIHSAAFALPTFLKREVRLLRDSP 359



 Score = 49.7 bits (117), Expect(2) = e-144
 Identities = 27/45 (60%), Positives = 31/45 (68%)
 Frame = +1

Query: 121 MEGDAGKGLGCQKITDGKVISDNNDSVRAIPPCCIKAMASAPELD 255
           ME   G+GL CQ+  DGKV +D N S   IP CC+KA ASAPELD
Sbjct: 1   MEDGVGRGLECQRTMDGKV-NDWNGS-EDIPSCCLKARASAPELD 43


>ref|XP_004145928.1| PREDICTED: spermine synthase-like [Cucumis sativus]
            gi|449497322|ref|XP_004160371.1| PREDICTED: spermine
            synthase-like [Cucumis sativus]
          Length = 365

 Score =  486 bits (1251), Expect(2) = e-144
 Identities = 230/296 (77%), Positives = 257/296 (86%)
 Frame = +3

Query: 363  DGSRRSYYNNPMWPGEAHSLQAEKILFKEKSEYQEILVFESVTYGKVLVLDGIVQLTEKD 542
            DG +R Y+NNPMWPGEAHSLQ E ILFK KSE+QE++VFES TYGKVLVLDGIVQLTEKD
Sbjct: 65   DGKKRVYFNNPMWPGEAHSLQVESILFKGKSEFQEVVVFESTTYGKVLVLDGIVQLTEKD 124

Query: 543  ECAYQEMIVHLPLCSISSPKNXXXXXXXXXXXXREISRHRSVKCIDICEIDKMVIDVSKK 722
            ECAYQEMI HLPLCSI SPK              EISRH S++ IDICEID+MVIDVSK+
Sbjct: 125  ECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSIEHIDICEIDQMVIDVSKE 184

Query: 723  FFPNLSVGFEDPRVRLHVGDAVEFLRNAPEGMYDAIIVDSSDPVGPAQELVERPFFEMIA 902
            FFP+L++GFEDPRV LHVGDAVEFLR AP G YDAIIVDSSDPVGPAQELVE+PFFE +A
Sbjct: 185  FFPDLAIGFEDPRVHLHVGDAVEFLRRAPRGKYDAIIVDSSDPVGPAQELVEKPFFETLA 244

Query: 903  RALRPGGVLCNMAESMWLHTHLIQDMISICRQIFKGSVHYAWTNVPTYPSGVIGFLICST 1082
            +AL+PGGVLCNMAESMWLHTHLI DMISICR++FKGS+HYAW +VPTYPSGVIGFL+CST
Sbjct: 245  KALKPGGVLCNMAESMWLHTHLIDDMISICREVFKGSIHYAWASVPTYPSGVIGFLLCST 304

Query: 1083 AGPPVDFLNPINPIEKIEGALKFNRELRFYNSEMHVAAFALPSFLRREVSILRDSP 1250
             GPPVDF NPINPIEK+EGA+K  ++L+FYNSEMH AAFALPSFLR+EV  L DSP
Sbjct: 305  EGPPVDFKNPINPIEKLEGAVKHKKDLKFYNSEMHSAAFALPSFLRKEVKALVDSP 360



 Score = 52.8 bits (125), Expect(2) = e-144
 Identities = 25/45 (55%), Positives = 32/45 (71%)
 Frame = +1

Query: 121 MEGDAGKGLGCQKITDGKVISDNNDSVRAIPPCCIKAMASAPELD 255
           ME DAG+GLGCQK  DG+  S+   + + IP CC+KA AS PEL+
Sbjct: 1   MEADAGRGLGCQKTMDGRA-SNGILTKKEIPSCCLKARASVPELE 44


>dbj|BAE19758.1| spermine synthase [Malus sylvestris]
          Length = 369

 Score =  481 bits (1237), Expect(2) = e-143
 Identities = 232/294 (78%), Positives = 255/294 (86%)
 Frame = +3

Query: 372  RRSYYNNPMWPGEAHSLQAEKILFKEKSEYQEILVFESVTYGKVLVLDGIVQLTEKDECA 551
            ++ Y+NNPMWPGEAHSL+ E IL++ KSEYQEILVFES  YGKVLVLDGI+QL+EKDECA
Sbjct: 68   KKVYFNNPMWPGEAHSLKVENILYRGKSEYQEILVFESSIYGKVLVLDGIIQLSEKDECA 127

Query: 552  YQEMIVHLPLCSISSPKNXXXXXXXXXXXXREISRHRSVKCIDICEIDKMVIDVSKKFFP 731
            YQEMI HLPLCSISSPK             RE+SRH S++ IDICEIDKMVIDVSKKFFP
Sbjct: 128  YQEMIAHLPLCSISSPKTVLVVGGGDGGVLREVSRHPSIEHIDICEIDKMVIDVSKKFFP 187

Query: 732  NLSVGFEDPRVRLHVGDAVEFLRNAPEGMYDAIIVDSSDPVGPAQELVERPFFEMIARAL 911
             L+VGF+DPRV LHVGDA EFLR APEG YDA+IVDSSDPVGPAQELVE+PFFE IARAL
Sbjct: 188  QLAVGFQDPRVHLHVGDATEFLRLAPEGKYDAVIVDSSDPVGPAQELVEKPFFETIARAL 247

Query: 912  RPGGVLCNMAESMWLHTHLIQDMISICRQIFKGSVHYAWTNVPTYPSGVIGFLICSTAGP 1091
            RPGGVLCNM ESMWLHTHLIQDMIS+CRQIFKGSV+YAW +VPTYPSGVIGFL+CSTAGP
Sbjct: 248  RPGGVLCNMTESMWLHTHLIQDMISVCRQIFKGSVNYAWASVPTYPSGVIGFLLCSTAGP 307

Query: 1092 PVDFLNPINPIEKIEGALKFNRELRFYNSEMHVAAFALPSFLRREVSILRDSPT 1253
            PVDF NP+N IEK+E ALK  REL+FYNSEMH AAFALP FLRREVS LR+S T
Sbjct: 308  PVDFKNPVNSIEKLERALKHKRELKFYNSEMHSAAFALPPFLRREVSALRESST 361



 Score = 54.7 bits (130), Expect(2) = e-143
 Identities = 27/45 (60%), Positives = 32/45 (71%)
 Frame = +1

Query: 121 MEGDAGKGLGCQKITDGKVISDNNDSVRAIPPCCIKAMASAPELD 255
           ME  AG+GL CQK  DGK  S+ N S +AIP CC+KAMAS PE +
Sbjct: 1   MEDGAGRGLECQKTMDGKG-SNGNGSEKAIPSCCLKAMASTPEAE 44


>emb|CAM35498.1| spermine synthase [Lotus japonicus]
          Length = 361

 Score =  484 bits (1246), Expect(2) = e-141
 Identities = 229/289 (79%), Positives = 257/289 (88%)
 Frame = +3

Query: 378  SYYNNPMWPGEAHSLQAEKILFKEKSEYQEILVFESVTYGKVLVLDGIVQLTEKDECAYQ 557
            +Y+NNPMWPGEAHS++ +KIL+KEKSEYQE+LVFES  YGKVLVLDGIVQLTEKDECAYQ
Sbjct: 70   AYFNNPMWPGEAHSIKVDKILYKEKSEYQEVLVFESSIYGKVLVLDGIVQLTEKDECAYQ 129

Query: 558  EMIVHLPLCSISSPKNXXXXXXXXXXXXREISRHRSVKCIDICEIDKMVIDVSKKFFPNL 737
            EMI HLPLCSI+SP+             RE+SRH SVK IDICEIDKMVIDVS+KFFP L
Sbjct: 130  EMIAHLPLCSIASPETVLVVGGGDGGVLREVSRHSSVKRIDICEIDKMVIDVSRKFFPEL 189

Query: 738  SVGFEDPRVRLHVGDAVEFLRNAPEGMYDAIIVDSSDPVGPAQELVERPFFEMIARALRP 917
            +VGF+DPRV LHVGDAVEFLR  PEG YDAIIVDSSDPVGPAQELVE+PFFE +A+ALRP
Sbjct: 190  AVGFDDPRVHLHVGDAVEFLRCTPEGKYDAIIVDSSDPVGPAQELVEKPFFETLAKALRP 249

Query: 918  GGVLCNMAESMWLHTHLIQDMISICRQIFKGSVHYAWTNVPTYPSGVIGFLICSTAGPPV 1097
            GGVLCNMAESMWLHTHLIQDMIS+CR+ FKGSVHYAW +VPTYPSG+IGFL+CST GPPV
Sbjct: 250  GGVLCNMAESMWLHTHLIQDMISVCRETFKGSVHYAWASVPTYPSGLIGFLLCSTEGPPV 309

Query: 1098 DFLNPINPIEKIEGALKFNRELRFYNSEMHVAAFALPSFLRREVSILRD 1244
            DF+NPINPIEK+EGA K  R+LRFYNSEMH AAFALP+FL+REVS++RD
Sbjct: 310  DFVNPINPIEKLEGADKHKRKLRFYNSEMHTAAFALPAFLKREVSLIRD 358



 Score = 47.4 bits (111), Expect(2) = e-141
 Identities = 23/41 (56%), Positives = 29/41 (70%)
 Frame = +1

Query: 133 AGKGLGCQKITDGKVISDNNDSVRAIPPCCIKAMASAPELD 255
           AG+GL CQK  DGK  S+ N   + +P CC+KA+AS PELD
Sbjct: 5   AGRGLECQKTMDGKE-SNRNGLEKELPLCCLKAIASDPELD 44


>ref|XP_002318386.1| predicted protein [Populus trichocarpa] gi|222859059|gb|EEE96606.1|
            predicted protein [Populus trichocarpa]
          Length = 347

 Score =  482 bits (1241), Expect(2) = e-139
 Identities = 226/292 (77%), Positives = 261/292 (89%)
 Frame = +3

Query: 369  SRRSYYNNPMWPGEAHSLQAEKILFKEKSEYQEILVFESVTYGKVLVLDGIVQLTEKDEC 548
            S++ Y+NNPMWPGEAH+L+ +++L+K KSEYQE+LVFES +YGKVLVLDGIVQLTEKDEC
Sbjct: 53   SKKVYFNNPMWPGEAHTLEVKEVLYKGKSEYQEVLVFESTSYGKVLVLDGIVQLTEKDEC 112

Query: 549  AYQEMIVHLPLCSISSPKNXXXXXXXXXXXXREISRHRSVKCIDICEIDKMVIDVSKKFF 728
            AYQEMI HLPLCSI SPK             REISRH SV+ IDICEID+MVIDVSKKFF
Sbjct: 113  AYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHSSVEHIDICEIDQMVIDVSKKFF 172

Query: 729  PNLSVGFEDPRVRLHVGDAVEFLRNAPEGMYDAIIVDSSDPVGPAQELVERPFFEMIARA 908
            P L+VGFEDPRVRLHVGDAV+FLR+ PEG YDAIIVDSSDPVGPAQELVE+PFFE IARA
Sbjct: 173  PQLAVGFEDPRVRLHVGDAVDFLRSTPEGKYDAIIVDSSDPVGPAQELVEKPFFETIARA 232

Query: 909  LRPGGVLCNMAESMWLHTHLIQDMISICRQIFKGSVHYAWTNVPTYPSGVIGFLICSTAG 1088
            LRPGGVLCNMAESMWLHTHLI+DMISICR+ FKGSVHYAW +VPTYPSG+IGF++CST G
Sbjct: 233  LRPGGVLCNMAESMWLHTHLIEDMISICRETFKGSVHYAWASVPTYPSGLIGFVLCSTEG 292

Query: 1089 PPVDFLNPINPIEKIEGALKFNRELRFYNSEMHVAAFALPSFLRREVSILRD 1244
            PPV+FLNP+NPIEK+EGA K+ REL++YNSE+H AAFALP+FL++EVS+L+D
Sbjct: 293  PPVEFLNPVNPIEKLEGATKYKRELKYYNSEVHSAAFALPTFLKKEVSLLQD 344



 Score = 40.0 bits (92), Expect(2) = e-139
 Identities = 19/30 (63%), Positives = 23/30 (76%)
 Frame = +1

Query: 166 DGKVISDNNDSVRAIPPCCIKAMASAPELD 255
           DGK  ++ N S +AIP CC+KA ASAPELD
Sbjct: 2   DGKA-NNGNGSEKAIPSCCLKARASAPELD 30