BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00002779
         (595 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin...   171   9e-41
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi...   153   2e-35
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin...   150   2e-34
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro...   149   4e-34
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      134   9e-30

>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
           Short=EcTNMT gi|226897702|gb|ACO90222.1|
           tetrahydroprotoberberine N-methyltransferase
           [Eschscholzia californica]
          Length = 350

 Score =  171 bits (433), Expect = 9e-41
 Identities = 78/112 (69%), Positives = 95/112 (84%)
 Frame = -1

Query: 595 DEEDWYSNYIFPKGSVTIPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLRKLDKNID 416
           DE+DWYS +IFPKG VTI  +STLLYFQDDV+ +D WV NG HMAR+VE W +KLD+ I+
Sbjct: 239 DEDDWYSGFIFPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEAWRKKLDETIE 298

Query: 415 AVRKILEPCLGSKDAVDKVITHSRTFCIGAYEQFSCNDGQEWMISHILFRKK 260
           A R+ILEP LGSK+AV++VITH RTFCIG YEQFS N+G+EWMI+ ILF+KK
Sbjct: 299 AAREILEPGLGSKEAVNQVITHIRTFCIGGYEQFSYNNGEEWMITQILFKKK 350


>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
           Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
           tetrahydroprotoberberine N-methyltransferase [Papaver
           bracteatum]
          Length = 358

 Score =  153 bits (387), Expect = 2e-35
 Identities = 70/113 (61%), Positives = 90/113 (79%), Gaps = 1/113 (0%)
 Frame = -1

Query: 595 DEEDWYSNYIFPKGSVTIPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLRKLDKNID 416
           DE+DWYS +IFP G  TI  A++LLYFQDDV+VVD WV NG HMAR+V+ W + LDKN++
Sbjct: 246 DEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNME 305

Query: 415 AVRKILEPCL-GSKDAVDKVITHSRTFCIGAYEQFSCNDGQEWMISHILFRKK 260
           A ++IL P L GS +AV+ V+TH RTFC+G YEQFS NDG EWM++ +LF+KK
Sbjct: 306 AAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFKKK 358


>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
           Short=PsTNMT gi|67764091|gb|AAY79177.1|
           S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
           cis-N-methyltransferase [Papaver somniferum]
          Length = 358

 Score =  150 bits (378), Expect = 2e-34
 Identities = 68/113 (60%), Positives = 89/113 (78%), Gaps = 1/113 (0%)
 Frame = -1

Query: 595 DEEDWYSNYIFPKGSVTIPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLRKLDKNID 416
           DE+DWYS +IFP G  TI  A++LLYFQDDV+VVD WV NG HMAR+V+ W + LDKN++
Sbjct: 246 DEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNME 305

Query: 415 AVRKILEPCL-GSKDAVDKVITHSRTFCIGAYEQFSCNDGQEWMISHILFRKK 260
           A ++IL P L GS + V+ V+TH RTFC+G YEQFS N+G EWM++ +LF+KK
Sbjct: 306 AAKEILLPGLGGSHETVNGVVTHIRTFCMGGYEQFSMNNGDEWMVAQLLFKKK 358


>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
           N-methyltransferase 2; Short=PbTNMT2
           gi|226897730|gb|ACO90236.1| putative N-methyltransferase
           [Papaver bracteatum]
          Length = 358

 Score =  149 bits (376), Expect = 4e-34
 Identities = 70/113 (61%), Positives = 86/113 (76%), Gaps = 1/113 (0%)
 Frame = -1

Query: 595 DEEDWYSNYIFPKGSVTIPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLRKLDKNID 416
           DE+DWYS YIFP G  T   A +LLYFQDDV+VVD WV NG H ARTV+ W +KLDKN++
Sbjct: 246 DEDDWYSGYIFPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDAWRKKLDKNME 305

Query: 415 AVRKILEPCL-GSKDAVDKVITHSRTFCIGAYEQFSCNDGQEWMISHILFRKK 260
           AV++IL P L G+ +AV+ VITH RT C+G Y QFS NDG EWM + +LF+KK
Sbjct: 306 AVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDGDEWMNAQLLFKKK 358


>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  134 bits (338), Expect = 9e-30
 Identities = 54/112 (48%), Positives = 82/112 (73%)
 Frame = -1

Query: 595 DEEDWYSNYIFPKGSVTIPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLRKLDKNID 416
           D++DW++ Y+FP G++ IP AS  LYFQDDV+VV+ W  +GKH +RT EEWL++LD N+D
Sbjct: 247 DDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLD 306

Query: 415 AVRKILEPCLGSKDAVDKVITHSRTFCIGAYEQFSCNDGQEWMISHILFRKK 260
            ++ + E  +G+++   K+I + R FC+   E F  N+G+EWM SH+LF+KK
Sbjct: 307 VIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 358