BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00002769
         (1476 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   699   0.0  
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc...   692   0.0  
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra...   620   e-175
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe...   494   e-137
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   489   e-135

>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  699 bits (1803), Expect = 0.0
 Identities = 348/350 (99%), Positives = 349/350 (99%)
 Frame = +1

Query: 118  MAFHGKDDILDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP 297
            MAFHGKDD LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDI+DNNNQPMALADLASKLP
Sbjct: 1    MAFHGKDDALDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIVDNNNQPMALADLASKLP 60

Query: 298  VSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQ 477
            VSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQ
Sbjct: 61   VSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQ 120

Query: 478  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 657
            KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS
Sbjct: 121  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 180

Query: 658  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 837
            GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM
Sbjct: 181  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 240

Query: 838  FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 1017
            FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST
Sbjct: 241  FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 300

Query: 1018 RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 1167
            RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP
Sbjct: 301  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350


>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
            4'-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:3'-hydroxy-N-
            methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
            gi|9711916|dbj|BAB08005.1|
            S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
            4'-O-methyltransferase [Coptis japonica]
          Length = 350

 Score =  692 bits (1787), Expect = 0.0
 Identities = 345/350 (98%), Positives = 349/350 (99%)
 Frame = +1

Query: 118  MAFHGKDDILDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP 297
            MAFHGKDD+LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP
Sbjct: 1    MAFHGKDDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP 60

Query: 298  VSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQ 477
            VS+VNCDNLYRILRYLVKMEILRVEKSDDG+KKYALEPIATLLSRNAKRSMVPMILGMTQ
Sbjct: 61   VSDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQ 120

Query: 478  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 657
            KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS
Sbjct: 121  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 180

Query: 658  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 837
            GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYD PNIERIGGDM
Sbjct: 181  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDM 240

Query: 838  FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 1017
            FKSVPSAQAI+LKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST
Sbjct: 241  FKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 300

Query: 1018 RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 1167
            RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP
Sbjct: 301  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350


>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase [Thalictrum
            flavum subsp. glaucum]
          Length = 348

 Score =  620 bits (1599), Expect = e-175
 Identities = 312/350 (89%), Positives = 325/350 (92%), Gaps = 1/350 (0%)
 Frame = +1

Query: 121  AFHGKDDILDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPV 300
            AF GKD  +DIKAQAHVWKIIYGFADSLVLRCAVELGI DIIDNNNQP+ LADLASKLP+
Sbjct: 3    AFQGKDASVDIKAQAHVWKIIYGFADSLVLRCAVELGIFDIIDNNNQPIPLADLASKLPI 62

Query: 301  SNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQK 480
            SNVN DNL+RILRYLVKME+L     D    KYALEPIA L+ RN KRSMVPM+LGMTQK
Sbjct: 63   SNVNLDNLFRILRYLVKMELLSYAADD----KYALEPIAKLVLRNEKRSMVPMVLGMTQK 118

Query: 481  DFMTPWHSMKDGLSDNG-TAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 657
            DFMTPWHSMKDGL+DNG TAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS
Sbjct: 119  DFMTPWHSMKDGLTDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 178

Query: 658  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 837
            GS+DMFQ IDSLVDVGGGNGTT+KAIS AFPHIKCTLFDLPHVIANSYDHPNIERIGGDM
Sbjct: 179  GSKDMFQSIDSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 238

Query: 838  FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 1017
            FK +PSAQAILLKLILHDWNDEDSIKILKQCR AVPKDGGKVIIVDVALDE+SDHELSST
Sbjct: 239  FKGMPSAQAILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDEDSDHELSST 298

Query: 1018 RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 1167
            RLILDIDMLVNTGGKERTKE WEK+VK AGFSGCKIRHIAAIQSVIEVFP
Sbjct: 299  RLILDIDMLVNTGGKERTKEDWEKLVKCAGFSGCKIRHIAAIQSVIEVFP 348


>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
            californica]
          Length = 353

 Score =  494 bits (1273), Expect = e-137
 Identities = 238/345 (68%), Positives = 295/345 (85%), Gaps = 4/345 (1%)
 Frame = +1

Query: 145  LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSNVNCDNL 324
            +DIKAQAH+W IIYGFADSLVLR AVELGI DII NNN  + +++LASKLP+SNVN DNL
Sbjct: 9    VDIKAQAHLWNIIYGFADSLVLRSAVELGIADIIKNNNGSITVSELASKLPISNVNSDNL 68

Query: 325  YRILRYLVKMEILRVEKS--DDGE--KKYALEPIATLLSRNAKRSMVPMILGMTQKDFMT 492
            YR+LRYLV M IL+  KS  + GE  K Y+LEP+ +LL ++A+R+MVP++LGMTQ+DFM 
Sbjct: 69   YRVLRYLVHMGILKETKSTINGGEIKKLYSLEPVGSLLVKDAERNMVPIVLGMTQQDFMI 128

Query: 493  PWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDM 672
            PWH +K+GL +  TAFEK MGMT+WEYLEGHP+Q  LFN GM GETRLLT +LI   +D 
Sbjct: 129  PWHYIKEGLGEGSTAFEKGMGMTLWEYLEGHPEQGHLFNVGMEGETRLLTKTLIESCKDT 188

Query: 673  FQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKSVP 852
            F+G+ SLVDVGGGNGTT+KAIS+AFPHIKC+L+DLPHV+A+S+D PNIE+I GD+FK +P
Sbjct: 189  FEGLSSLVDVGGGNGTTIKAISEAFPHIKCSLYDLPHVVADSHDLPNIEKIPGDIFKFIP 248

Query: 853  SAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILD 1032
            +AQAILLKLILHDW+DEDS+KILK+CR AVP+D G+VIIVDVAL+EES+H L+ TRL+LD
Sbjct: 249  NAQAILLKLILHDWSDEDSVKILKKCREAVPQDTGRVIIVDVALEEESEHPLTKTRLVLD 308

Query: 1033 IDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 1167
            +DMLVNTGG+ER+++ W K++K AGF   KIRHIAA+QSVIE FP
Sbjct: 309  VDMLVNTGGRERSEDDWAKLLKLAGFRTHKIRHIAAVQSVIEAFP 353


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase 2 [Papaver somniferum]
          Length = 357

 Score =  489 bits (1258), Expect = e-135
 Identities = 241/343 (70%), Positives = 287/343 (83%), Gaps = 2/343 (0%)
 Frame = +1

Query: 145  LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSNVNCDNL 324
            + IK QA +W IIYGFADSLVLRCAVE+GI DII NN+  + LA LA+KLP++NV+ D L
Sbjct: 15   VSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYL 74

Query: 325  YRILRYLVKMEILRVEKSDDG-EKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWH 501
            YR++RYLV + I+  E  + G EK Y+L+P+ TLL R+A+RSMVPMILGMTQKDFM  WH
Sbjct: 75   YRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWH 134

Query: 502  SMKDGLSDNGT-AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQ 678
             MK+GL +  T AFEK MGM IW+YLEG+PDQSQLFNEGMAGETRLLT +LI   RD FQ
Sbjct: 135  FMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQ 194

Query: 679  GIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKSVPSA 858
            G+DSLVD+GGGNGTT+KAI +AFPHIKCTL+DLPHV+ANS+D PNIE++ GDMFKSVPSA
Sbjct: 195  GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSA 254

Query: 859  QAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDID 1038
            QAILLKLILHDW DE+ + ILK+C+ A+PK+ GKVIIVDVAL+EES+HEL+ TRLILDID
Sbjct: 255  QAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDID 314

Query: 1039 MLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 1167
            MLVNTGG+ERT + WE ++K AGF   KIR I AIQSVIE FP
Sbjct: 315  MLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357