BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00002734
         (1801 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast prec...   844   0.0  
ref|XP_002304493.1| predicted protein [Populus trichocarpa] gi|2...   838   0.0  
ref|XP_002298035.1| predicted protein [Populus trichocarpa] gi|2...   837   0.0  
gb|AFF18844.2| gamma-glutamylcysteine synthetase [Dimocarpus lon...   836   0.0  
gb|ABK96626.1| unknown [Populus trichocarpa x Populus deltoides]      835   0.0  

>ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast precursor, putative [Ricinus
            communis] gi|223549699|gb|EEF51187.1| Glutamate--cysteine
            ligase, chloroplast precursor, putative [Ricinus
            communis]
          Length = 526

 Score =  844 bits (2180), Expect = 0.0
 Identities = 404/463 (87%), Positives = 429/463 (92%)
 Frame = -2

Query: 1626 NKTTSSKRGNRFVVAASPPTEDAVIATEPLTKQDLVNYLASGCKPKDQWRIGTEHEKFGF 1447
            N    S+RGN+ +VAASPP EDAVIATEPLTK+DLV YLASGCKPK++WRIGTEHEKFGF
Sbjct: 64   NSWVRSRRGNQMIVAASPPIEDAVIATEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGF 123

Query: 1446 ELDTLRPMKYEQIADLLYGLAERFDWEKIMEGDYIIGLKQGKQSVSLEPGGQFELSGAPL 1267
            EL TLRPMKYEQIA+LL G+AERFDWEK+MEGDYIIGLKQGKQS+SLEPGGQFELSGAPL
Sbjct: 124  ELGTLRPMKYEQIAELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPL 183

Query: 1266 ETLHQTCAEVNSHLYQVKAVAEEXXXXXXXXXFQPKWRIKDIPIMPKGRYDIMRNYMPKA 1087
            ETLHQTCAEVNSHLYQVKAV EE         FQPKW +KDIPIMPKGRY+IMRNYMPK 
Sbjct: 184  ETLHQTCAEVNSHLYQVKAVTEEMGIGFLGIGFQPKWGVKDIPIMPKGRYEIMRNYMPKV 243

Query: 1086 GSLGLDMMFRTCTVQVNLDFSSESDMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSM 907
            G+LGLDMMFRTCTVQVNLDFSSE+DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSM
Sbjct: 244  GTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSM 303

Query: 906  RSHIWSDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRNKKYIDCTGLSFRDFLE 727
            RS IW+DTD +RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYR KKYIDCTG++FRDFL 
Sbjct: 304  RSQIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLA 363

Query: 726  GKLPAAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDE 547
            GKLP  PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDE
Sbjct: 364  GKLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDE 423

Query: 546  VSLQNVLDMTADWTSAERQMLRNKVPMTGLKTPFRDGLLRHVAEDVLKLAKDGLERRGYK 367
            +SLQNVLDMTADWT  ER+MLRNKVP TGLKTPFRDGLL+HVAEDVLKLAKDGLERRGYK
Sbjct: 424  ISLQNVLDMTADWTPEEREMLRNKVPKTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYK 483

Query: 366  ETGFLNALTEVVTTGVTPAEKLLELYEGKWGRSVDPVFEELLY 238
            E GFLN +TEVV TGVTPAE+LLELY GKWG+SVDPVFEELLY
Sbjct: 484  EIGFLNEVTEVVRTGVTPAERLLELYNGKWGQSVDPVFEELLY 526


>ref|XP_002304493.1| predicted protein [Populus trichocarpa] gi|222841925|gb|EEE79472.1|
            predicted protein [Populus trichocarpa]
          Length = 526

 Score =  838 bits (2166), Expect = 0.0
 Identities = 401/478 (83%), Positives = 436/478 (91%), Gaps = 1/478 (0%)
 Frame = -2

Query: 1668 IQCNKNVEKKVVFRN-KTTSSKRGNRFVVAASPPTEDAVIATEPLTKQDLVNYLASGCKP 1492
            + CN +   K++     +   KRG++FVVAASPPTEDAV+ATEPLTK+DL+ YLASGCKP
Sbjct: 49   LSCNSSKTPKILSSECLSVGMKRGHQFVVAASPPTEDAVVATEPLTKEDLIRYLASGCKP 108

Query: 1491 KDQWRIGTEHEKFGFELDTLRPMKYEQIADLLYGLAERFDWEKIMEGDYIIGLKQGKQSV 1312
            K+ WRIGTEHEKFGFE+ TLRPMKYEQIA+LL+G+AERFDW+KIMEGD IIGLKQ KQS+
Sbjct: 109  KENWRIGTEHEKFGFEIGTLRPMKYEQIAELLHGIAERFDWDKIMEGDTIIGLKQEKQSI 168

Query: 1311 SLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEXXXXXXXXXFQPKWRIKDIPIM 1132
            SLEPGGQFELSGAP+ETLHQTCAEVNSHLYQVKAVAEE         FQPKW +KDIPIM
Sbjct: 169  SLEPGGQFELSGAPVETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWVLKDIPIM 228

Query: 1131 PKGRYDIMRNYMPKAGSLGLDMMFRTCTVQVNLDFSSESDMIRKFRAGLALQPIATALFA 952
            PKGRYDIMRNYMPK GSLGLDMMFRTCTVQVNLDFSSE+DMIRKFRAGLALQPIATALFA
Sbjct: 229  PKGRYDIMRNYMPKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFA 288

Query: 951  NSPFTEGKPNGYLSMRSHIWSDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRNK 772
            NSPFT+GKPNGYLS RSHIW+DTDNNR GMLPFVFDD+FGFEQYVDYALDVPMYFVYR +
Sbjct: 289  NSPFTDGKPNGYLSKRSHIWTDTDNNRAGMLPFVFDDNFGFEQYVDYALDVPMYFVYRKE 348

Query: 771  KYIDCTGLSFRDFLEGKLPAAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRR 592
            KYIDCTG+SFRDFL G+LP  PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRR
Sbjct: 349  KYIDCTGMSFRDFLAGRLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRR 408

Query: 591  LCALPAFWVGILYDEVSLQNVLDMTADWTSAERQMLRNKVPMTGLKTPFRDGLLRHVAED 412
            LCALPAFWVG+LYDE SLQ+VLDMTADWTS ERQMLRNKVP TGLKTPFRDGLLRHVAE+
Sbjct: 409  LCALPAFWVGLLYDEDSLQSVLDMTADWTSEERQMLRNKVPKTGLKTPFRDGLLRHVAEE 468

Query: 411  VLKLAKDGLERRGYKETGFLNALTEVVTTGVTPAEKLLELYEGKWGRSVDPVFEELLY 238
            VLKLAKDGLERRG+KE GFLNA+ EV +TG+TPAEKLLELY GKWG+S+DPVFEELLY
Sbjct: 469  VLKLAKDGLERRGFKEVGFLNAVAEVASTGITPAEKLLELYHGKWGQSIDPVFEELLY 526


>ref|XP_002298035.1| predicted protein [Populus trichocarpa] gi|222845293|gb|EEE82840.1|
            predicted protein [Populus trichocarpa]
          Length = 526

 Score =  837 bits (2161), Expect = 0.0
 Identities = 400/478 (83%), Positives = 436/478 (91%), Gaps = 1/478 (0%)
 Frame = -2

Query: 1668 IQCNKNVEKKVVFRNK-TTSSKRGNRFVVAASPPTEDAVIATEPLTKQDLVNYLASGCKP 1492
            + CN +   +++     +   KRG+R VVAASPPTEDAVIATEPLTK+DL+ YLASGCKP
Sbjct: 49   LSCNSSKTPQILSSESLSVGMKRGHRVVVAASPPTEDAVIATEPLTKEDLIGYLASGCKP 108

Query: 1491 KDQWRIGTEHEKFGFELDTLRPMKYEQIADLLYGLAERFDWEKIMEGDYIIGLKQGKQSV 1312
            K+ WRIGTEHEKFGFE+ TLRPMKYEQIA LL+G+AERFDW++IMEGD IIGL+QGKQS+
Sbjct: 109  KENWRIGTEHEKFGFEIGTLRPMKYEQIAGLLHGIAERFDWDRIMEGDNIIGLQQGKQSI 168

Query: 1311 SLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEXXXXXXXXXFQPKWRIKDIPIM 1132
            SLEPGGQFELSGAP+ETLHQTCAEVNSHLYQVKAVAEE         FQPKW +KDIP+M
Sbjct: 169  SLEPGGQFELSGAPVETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGLKDIPVM 228

Query: 1131 PKGRYDIMRNYMPKAGSLGLDMMFRTCTVQVNLDFSSESDMIRKFRAGLALQPIATALFA 952
            PKGRYDIMRNYMPK GSLGLDMMFRTCTVQVNLDF SE+DMIRKFRAGLALQPIATALFA
Sbjct: 229  PKGRYDIMRNYMPKVGSLGLDMMFRTCTVQVNLDFISEADMIRKFRAGLALQPIATALFA 288

Query: 951  NSPFTEGKPNGYLSMRSHIWSDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRNK 772
            NSPFTEGKPNGYLS+RSHIW+DTDNNR GMLPFVF+D+FGFEQYVDYALDVPMYFVYR K
Sbjct: 289  NSPFTEGKPNGYLSLRSHIWTDTDNNRAGMLPFVFNDTFGFEQYVDYALDVPMYFVYRKK 348

Query: 771  KYIDCTGLSFRDFLEGKLPAAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRR 592
            KYIDCTG+SFRDFL GKLP  PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRR
Sbjct: 349  KYIDCTGMSFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRR 408

Query: 591  LCALPAFWVGILYDEVSLQNVLDMTADWTSAERQMLRNKVPMTGLKTPFRDGLLRHVAED 412
            LCALPAFWVG+LYDE SLQ+VLDMTADWTS ERQMLRNKVP TGLKTPFR GLLRHVAE+
Sbjct: 409  LCALPAFWVGLLYDEDSLQSVLDMTADWTSEERQMLRNKVPKTGLKTPFRGGLLRHVAEE 468

Query: 411  VLKLAKDGLERRGYKETGFLNALTEVVTTGVTPAEKLLELYEGKWGRSVDPVFEELLY 238
            VLKLAKDGLERRG+KE GFLNA+++VV+TG+TPAEKLLELY GKWGRS+DPVFEELLY
Sbjct: 469  VLKLAKDGLERRGFKEVGFLNAVSDVVSTGITPAEKLLELYHGKWGRSIDPVFEELLY 526


>gb|AFF18844.2| gamma-glutamylcysteine synthetase [Dimocarpus longan]
          Length = 511

 Score =  836 bits (2160), Expect = 0.0
 Identities = 413/512 (80%), Positives = 448/512 (87%), Gaps = 17/512 (3%)
 Frame = -2

Query: 1722 MALISKIGCSGYLFGSEI-------------IQCNKNVEKKVVFRN----KTTSSKRGNR 1594
            MAL+S+ G + Y   SEI             ++ ++  E  V F +     T  S+RG R
Sbjct: 1    MALVSQGGAT-YRVRSEITPGHRGVFGLVNNVEASRVKESCVSFSSLSCYSTKGSRRGYR 59

Query: 1593 FVVAASPPTEDAVIATEPLTKQDLVNYLASGCKPKDQWRIGTEHEKFGFELDTLRPMKYE 1414
             +VAASPPT+DAVIA EPLTK+DLV YLASGCKP+++WRIGTEHEKFGFE+ TLRPMKYE
Sbjct: 60   AIVAASPPTDDAVIAAEPLTKEDLVGYLASGCKPREKWRIGTEHEKFGFEIGTLRPMKYE 119

Query: 1413 QIADLLYGLAERFDWEKIMEGDYIIGLKQGKQSVSLEPGGQFELSGAPLETLHQTCAEVN 1234
            QIA+LL  +AERFDWEKIME + IIGLKQGKQS+SLEPGGQFELSGAPLETLHQTCAEVN
Sbjct: 120  QIAELLNSIAERFDWEKIMEDNNIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVN 179

Query: 1233 SHLYQVKAVAEEXXXXXXXXXFQPKWRIKDIPIMPKGRYDIMRNYMPKAGSLGLDMMFRT 1054
            SHLYQVKAVAEE         FQPKW IKDIP+MPKGRY+IM+NYMPK GSLGLDMMFRT
Sbjct: 180  SHLYQVKAVAEEMGIGFLGIGFQPKWGIKDIPVMPKGRYEIMKNYMPKVGSLGLDMMFRT 239

Query: 1053 CTVQVNLDFSSESDMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWSDTDNN 874
            CTVQVNLDFSSE+DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRS IW+DTDNN
Sbjct: 240  CTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQIWTDTDNN 299

Query: 873  RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRNKKYIDCTGLSFRDFLEGKLPAAPGELP 694
            RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYR KKYIDCTG+SFRDFL GKLP  PGELP
Sbjct: 300  RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMSFRDFLVGKLPCLPGELP 359

Query: 693  TLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQNVLDMTA 514
            TLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDEVSLQN LD+TA
Sbjct: 360  TLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEVSLQNALDLTA 419

Query: 513  DWTSAERQMLRNKVPMTGLKTPFRDGLLRHVAEDVLKLAKDGLERRGYKETGFLNALTEV 334
            DWTS ERQMLRNKVP TGLKTPFRDGLLRHVA+D+LKLAKDGLERRG+KE+GFLNA+ EV
Sbjct: 420  DWTSEERQMLRNKVPKTGLKTPFRDGLLRHVAQDILKLAKDGLERRGFKESGFLNAVAEV 479

Query: 333  VTTGVTPAEKLLELYEGKWGRSVDPVFEELLY 238
            V TGVTPAEKLLE+Y GKWG+SVDPVFEELLY
Sbjct: 480  VRTGVTPAEKLLEMYHGKWGQSVDPVFEELLY 511


>gb|ABK96626.1| unknown [Populus trichocarpa x Populus deltoides]
          Length = 526

 Score =  835 bits (2156), Expect = 0.0
 Identities = 399/478 (83%), Positives = 434/478 (90%), Gaps = 1/478 (0%)
 Frame = -2

Query: 1668 IQCNKNVEKKVVFRN-KTTSSKRGNRFVVAASPPTEDAVIATEPLTKQDLVNYLASGCKP 1492
            + CN +   K++     +   KRG++FVVAASPPTEDAV+ATEPLTK+DL+ YLASGCKP
Sbjct: 49   LSCNSSKTPKILSSECLSVGMKRGHQFVVAASPPTEDAVVATEPLTKEDLIRYLASGCKP 108

Query: 1491 KDQWRIGTEHEKFGFELDTLRPMKYEQIADLLYGLAERFDWEKIMEGDYIIGLKQGKQSV 1312
            K+ WRIGTEHEKFGFE+ TLRPMKYEQIA+LL+G+AERFDW+KIMEGD IIGLKQ KQS+
Sbjct: 109  KENWRIGTEHEKFGFEIGTLRPMKYEQIAELLHGIAERFDWDKIMEGDTIIGLKQEKQSI 168

Query: 1311 SLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEXXXXXXXXXFQPKWRIKDIPIM 1132
            SLEPGGQFELSGAP+ETLHQTCAEVNSHLYQVKAVAEE         FQPKW +KDIPIM
Sbjct: 169  SLEPGGQFELSGAPVETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWVLKDIPIM 228

Query: 1131 PKGRYDIMRNYMPKAGSLGLDMMFRTCTVQVNLDFSSESDMIRKFRAGLALQPIATALFA 952
            PKGRYDIMRNYMPK GSLGLDMMFRTCTVQVNLDFSSE+DMIRKFRAGLALQPIATALFA
Sbjct: 229  PKGRYDIMRNYMPKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFA 288

Query: 951  NSPFTEGKPNGYLSMRSHIWSDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRNK 772
            NSPFT+GKPNGYLS RSHIW+DTDNNR G LPFVFDD+FGFEQYVDYALDVPMYFVYR +
Sbjct: 289  NSPFTDGKPNGYLSKRSHIWTDTDNNRAGKLPFVFDDNFGFEQYVDYALDVPMYFVYRKE 348

Query: 771  KYIDCTGLSFRDFLEGKLPAAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRR 592
            KYIDCTG+SFRDFL G+LP  PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRR
Sbjct: 349  KYIDCTGMSFRDFLAGRLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRR 408

Query: 591  LCALPAFWVGILYDEVSLQNVLDMTADWTSAERQMLRNKVPMTGLKTPFRDGLLRHVAED 412
            LCALPAFWVG+LYDE SLQ+VLDMTADWTS ERQMLRNKVP TGLKTPFRDGLLRHVAE+
Sbjct: 409  LCALPAFWVGLLYDEDSLQSVLDMTADWTSEERQMLRNKVPKTGLKTPFRDGLLRHVAEE 468

Query: 411  VLKLAKDGLERRGYKETGFLNALTEVVTTGVTPAEKLLELYEGKWGRSVDPVFEELLY 238
            VLKLAKDGLERRG+KE GFLNA+ EV +TG+TPAEKLLELY GKWG+S+DP FEELLY
Sbjct: 469  VLKLAKDGLERRGFKEVGFLNAVAEVASTGITPAEKLLELYHGKWGQSIDPAFEELLY 526