BLASTX nr result
ID: Coptis23_contig00002592
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00002592
(1451 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 503 e-140
ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 458 e-126
ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|2... 453 e-125
ref|XP_004136614.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 422 e-115
ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabid... 415 e-113
>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
gi|297739656|emb|CBI29838.3| unnamed protein product
[Vitis vinifera]
Length = 468
Score = 503 bits (1294), Expect = e-140
Identities = 273/469 (58%), Positives = 331/469 (70%), Gaps = 35/469 (7%)
Frame = +2
Query: 11 AGACGSGCYK----------------AXXXXXXXXXXNTGRKCYKCKVAKFIESTGGL-- 136
AG C SGC++ N C KCKV + + S+G +
Sbjct: 5 AGNCQSGCFRDDDDSVVTEKKKLTNRTLSSGSDIDLPNHHNLCLKCKVNETLASSGTVSV 64
Query: 137 -------CGECLDRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPSSRVALEFVHEMQNKA 295
C +C +LF KFK AV++ +ISPTDNVL+AFSGGPSSRVAL+FVHEMQ+KA
Sbjct: 65 DGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSGGPSSRVALQFVHEMQHKA 124
Query: 296 QKNRDASRDKSYPVFGVGVAFIDETAVTSL---QLDKAIQDLRMIVSSLAPPRKELHIMP 466
QKN DASRD+S PVFGVGVAF+DE+AV+ + + DKAIQD++ IVS+LAPP K H++P
Sbjct: 125 QKNFDASRDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQDMKSIVSNLAPPDKAFHVVP 184
Query: 467 IQTLLPDDRDDAVNRLKELLLTVTDVTGKEDLLHHLRMLCLQKVALENNYNKLILGSCTS 646
I+++ + D RL+ELL V DVTGKEDLL HLRML LQK A EN Y KL+LGSCTS
Sbjct: 185 IESVYSSNGRD---RLEELLNAVKDVTGKEDLLQHLRMLSLQKNAYENGYTKLLLGSCTS 241
Query: 647 RIACHVLSATVKGQGYSLPADVQYADARWEIPVVLPLHDCVSQELNMLCRLNSLKTLDSF 826
RIACHV+SATVKGQGYSLPAD+QY DARWEIPVVLPL DC++QELNMLCRL+ LKT++
Sbjct: 242 RIACHVISATVKGQGYSLPADIQYVDARWEIPVVLPLRDCLAQELNMLCRLDGLKTMEVL 301
Query: 827 DSPHSGINELVSTFITLLTEENPARERTIVRTAEKLVPFQFNRINESLDS----GDRRRR 994
+SPHSGIN LV++F+TLL EENP+RE TIVRTA KL PF FN+I ES DS RRR+
Sbjct: 302 NSPHSGINGLVASFVTLLQEENPSRECTIVRTAGKLTPFHFNQIPESDDSNVHLASRRRQ 361
Query: 995 RKVNLKSYESISSEFLCPICCSPV---NNLNLEYLKSKLESCATSPTVFGDSCCPSCQFQ 1165
+K NLK ES+ E CPIC SP+ +++NL S L S T+ +FG +CCPSCQFQ
Sbjct: 362 KKFNLKPNESVPPESYCPICNSPLSKSDHINL----SSLGSDQTNADIFGATCCPSCQFQ 417
Query: 1166 ILPKESSSMEHFYSLVPQQMITRVKDSVGGDQAWLREQIEDCLLSDGED 1312
ILPKE SMEHFYSL+PQQ + + KD Q LREQI+D LLSD ED
Sbjct: 418 ILPKEPVSMEHFYSLLPQQFVVQAKDRSFSMQRQLREQIQDFLLSDSED 466
>ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Glycine max]
Length = 458
Score = 458 bits (1178), Expect = e-126
Identities = 242/454 (53%), Positives = 315/454 (69%), Gaps = 19/454 (4%)
Frame = +2
Query: 11 AGACGSGCYK-------AXXXXXXXXXXNTGRKCYKCKVAKFIESTGGL-----CGECLD 154
+G GCYK N C KCK+ + GG+ C +C
Sbjct: 6 SGCQSGGCYKDEDATCKQPPKSEEADSKNPSNVCIKCKLNDAVSGYGGIDDGRFCADCFK 65
Query: 155 RSLFAKFKQAVSTQNLISPTDNVLLAFSGGPSSRVALEFVHEMQNKAQKNRDASRDKSYP 334
+LF KF+ AV++ +I+PTD VL+AFSGGPSSRVAL+FVH+MQ +AQ+N DASRD+S P
Sbjct: 66 TNLFGKFRFAVTSNAMITPTDKVLVAFSGGPSSRVALQFVHDMQERAQRNFDASRDRSLP 125
Query: 335 VFGVGVAFIDETAVTSL---QLDKAIQDLRMIVSSLAPPRKELHIMPIQTLLPDDRDDAV 505
VFGVGV FIDE+AV + ++++A++ + +VSSLAPPRKELHI+PI+T+ D D
Sbjct: 126 VFGVGVVFIDESAVLPIPSNEMEEAVEVVSEVVSSLAPPRKELHIVPIETVYSSDSGDGK 185
Query: 506 NRLKELLLTVTDVTGKEDLLHHLRMLCLQKVALENNYNKLILGSCTSRIACHVLSATVKG 685
R+ +++ TV D TG+ED+L LRML LQKVA E YN++ILGSC SRIACHV+SATVKG
Sbjct: 186 ERITKVVNTVIDPTGREDVLLCLRMLALQKVASEFGYNRIILGSCISRIACHVISATVKG 245
Query: 686 QGYSLPADVQYADARWEIPVVLPLHDCVSQELNMLCRLNSLKTLDSFDSPHSGINELVST 865
QGYSLPAD+QY DARWE+PVVLPL DC +QE+NMLC L+ LKT+ S IN LVS+
Sbjct: 246 QGYSLPADIQYVDARWEVPVVLPLRDCFAQEINMLCHLDGLKTVKLSTDLCSSINGLVSS 305
Query: 866 FITLLTEENPARERTIVRTAEKLVPFQFNRINESLDS----GDRRRRRKVNLKSYESISS 1033
F+ LL EENP+RE TIVRTA KL PFQFNRI E +D RRR+++ NLKS ES+SS
Sbjct: 306 FVALLQEENPSRESTIVRTAGKLTPFQFNRIPEIIDGNVPLATRRRQKRYNLKSNESVSS 365
Query: 1034 EFLCPICCSPVNNLNLEYLKSKLESCATSPTVFGDSCCPSCQFQILPKESSSMEHFYSLV 1213
E CP+C SP++ + S ++S +S +F ++CC SC+FQILP +S +ME FY +
Sbjct: 366 ESFCPLCNSPLDKSEITDW-SNIDSHRSS-DIFYNTCCSSCRFQILPSDSMAMEQFYMDL 423
Query: 1214 PQQMITRVKDSVGGDQAWLREQIEDCLLSDGEDE 1315
PQ ++ R K + G+ + LREQI+D LLSDGEDE
Sbjct: 424 PQSVVGRAKQANNGNLSLLREQIQDYLLSDGEDE 457
>ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|222854006|gb|EEE91553.1|
predicted protein [Populus trichocarpa]
Length = 443
Score = 453 bits (1166), Expect = e-125
Identities = 243/431 (56%), Positives = 304/431 (70%), Gaps = 21/431 (4%)
Frame = +2
Query: 11 AGACGSGCYK--------AXXXXXXXXXXNTGRKCYKCKVAKFIE--STGG----LCGEC 148
+ C S CYK N+ C KCK I S G C +C
Sbjct: 5 SSGCQSNCYKNGDQVSDSPPPPPTLAAANNSNNLCLKCKTNSPITPGSLSGDEARFCADC 64
Query: 149 LDRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPSSRVALEFVHEMQNKAQKNRDASRDKS 328
+++ KF+ AV++Q +ISP+DNVL+AFSGGPSSRVAL+FVH +Q++AQKN +AS+DKS
Sbjct: 65 FRSNIYGKFRLAVTSQAMISPSDNVLVAFSGGPSSRVALQFVHGLQDRAQKNFEASKDKS 124
Query: 329 YPVFGVGVAFIDETAVTSLQLDK---AIQDLRMIVSSLAPPRKELHIMPIQTLLPDDRDD 499
PVFGVGVAFIDE+AV + DK AIQD+R +VS+LAPP K+LH++PI+ + D D
Sbjct: 125 LPVFGVGVAFIDESAVYPVSSDKVDGAIQDMRSVVSNLAPPTKQLHVVPIENIYASDSSD 184
Query: 500 AVNRLKELLLTVTDVTGKEDLLHHLRMLCLQKVALENNYNKLILGSCTSRIACHVLSATV 679
+RL +LL V D TGKEDLL HLRML LQ VA +N YN+LILGSCTSRIACHVL+ATV
Sbjct: 185 GKDRLTKLLDAVNDATGKEDLLLHLRMLALQMVASQNGYNRLILGSCTSRIACHVLAATV 244
Query: 680 KGQGYSLPADVQYADARWEIPVVLPLHDCVSQELNMLCRLNSLKTLDSFDSPHSGINELV 859
KGQGYSL AD+QY DARWEIPVVLPL DC SQELN+LC+L+ LKTL FDS SGIN L+
Sbjct: 245 KGQGYSLSADIQYVDARWEIPVVLPLRDCTSQELNLLCQLDGLKTLQPFDSTPSGINGLI 304
Query: 860 STFITLLTEENPARERTIVRTAEKLVPFQFNRINE----SLDSGDRRRRRKVNLKSYESI 1027
S+F+ +L EEN +RE TI+RTA KL PF+FNRI E ++ RRR+++ NLK+ ESI
Sbjct: 305 SSFVNVLQEENSSRECTILRTAGKLTPFEFNRIPETDNCNVPLAIRRRQKRYNLKTKESI 364
Query: 1028 SSEFLCPICCSPVNNLNLEYLKSKLESCATSPTVFGDSCCPSCQFQILPKESSSMEHFYS 1207
SSE CPIC +P+ N N L S L+SC +S F +CC SCQFQI+PK+ SSM +F S
Sbjct: 365 SSELFCPICNAPLKNFNSLNL-SSLKSCQSSR--FNAACCSSCQFQIIPKDPSSMGNFCS 421
Query: 1208 LVPQQMITRVK 1240
L+PQQ++ R K
Sbjct: 422 LLPQQVVDRAK 432
>ref|XP_004136614.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Cucumis
sativus]
Length = 469
Score = 422 bits (1084), Expect = e-115
Identities = 221/422 (52%), Positives = 298/422 (70%), Gaps = 13/422 (3%)
Frame = +2
Query: 89 CYKCKVAKFIESTG-----GLCGECLDRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPSS 253
C+KCK + + ++G C +C +LFAKF+QAV++ +ISP+D VL+AFSGGP S
Sbjct: 55 CFKCKSKERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCS 114
Query: 254 RVALEFVHEMQNKAQKNRDASRDKSYPVFGVGVAFIDETAVTSL---QLDKAIQDLRMIV 424
RVAL+FVHEMQ KA N +ASRD+S VFGVGVA+I+ETA S+ ++DK +Q ++ +V
Sbjct: 115 RVALQFVHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVV 174
Query: 425 SSLAPPRKELHIMPIQTLLPDDRDDAVNRLKELLLTVTDVTGKEDLLHHLRMLCLQKVAL 604
SSL PRK LHI+ I+++ + +D +LK+LL V DVTGKEDL+ +LRML LQK+A
Sbjct: 175 SSLCQPRKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAA 234
Query: 605 ENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADVQYADARWEIPVVLPLHDCVSQELN 784
EN YN+L++G+CTSRIACHV+S+TVKGQGYSLP DVQY DARWE+PVVLPL DC++QELN
Sbjct: 235 ENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELN 294
Query: 785 MLCRLNSLKTLDSFDSPHSGINELVSTFITLLTEENPARERTIVRTAEKLVPFQFNRINE 964
MLC ++ LKT++ SGIN+LVS+F+ LL ENP+RE TIVRTA KL PF F+RI +
Sbjct: 295 MLCCVDGLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPD 354
Query: 965 SLDSGD-----RRRRRKVNLKSYESISSEFLCPICCSPVNNLNLEYLKSKLESCATSPTV 1129
+S + +RR+++ N K S+SSE C +C P+N S + + ++
Sbjct: 355 LNESNNVPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNT-------SDVLTRHAGSSI 407
Query: 1130 FGDSCCPSCQFQILPKESSSMEHFYSLVPQQMITRVKDSVGGDQAWLREQIEDCLLSDGE 1309
G +CC SCQFQILPK+SSS E F SL+P M+ R G+ LR++I++ LLSD E
Sbjct: 408 LG-ACCSSCQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDE 466
Query: 1310 DE 1315
E
Sbjct: 467 AE 468
>ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabidopsis thaliana]
gi|332278212|sp|O65628.3|CTU2_ARATH RecName:
Full=Cytoplasmic tRNA 2-thiolation protein 2
gi|115646788|gb|ABJ17118.1| At4g35910 [Arabidopsis
thaliana] gi|332661188|gb|AEE86588.1| cytoplasmic tRNA
2-thiolation protein 2 [Arabidopsis thaliana]
Length = 458
Score = 415 bits (1066), Expect = e-113
Identities = 228/456 (50%), Positives = 303/456 (66%), Gaps = 20/456 (4%)
Frame = +2
Query: 11 AGACGSGCY-------KAXXXXXXXXXXNTGRKCYKCKV-AKFIESTGGL-----CGECL 151
+ C SGCY N C KCK A GG C +C
Sbjct: 5 SSGCESGCYDREKDNGSKIVDDAVSGGGNHESVCVKCKCNAPMTFGDGGFDDGRFCADCF 64
Query: 152 DRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPSSRVALEFVHEMQNKAQKNRDASRDKSY 331
++F KF+ AV++ +I+P+DNVL+AFSGG SSRV+L+FVHE+Q KA KN +ASRD+S
Sbjct: 65 RNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQFVHELQIKALKNYEASRDRSL 124
Query: 332 PVFGVGVAFIDETAV---TSLQLDKAIQDLRMIVSSLAPPRKELHIMPIQTLLPDDRDDA 502
PVFGVGVAF+DETA S ++ AI+ +R VS L+PP K+LH++P++++ D DA
Sbjct: 125 PVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPPAKDLHVVPVESIFGSDSLDA 184
Query: 503 VNRLKELLLTVTDVTGKEDLLHHLRMLCLQKVALENNYNKLILGSCTSRIACHVLSATVK 682
+RL +LL +V D TGKEDLL HL+ML LQKVA EN YN+L+LGSCTSRIA HVL+ATVK
Sbjct: 185 RDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNRLVLGSCTSRIASHVLTATVK 244
Query: 683 GQGYSLPADVQYADARWEIPVVLPLHDCVSQELNMLCRLNSLKTLDSFDSPHSGINELVS 862
G+GYSL AD+Q+ DARW++P+VLPL DCV E+ LC L+ LKT++ GIN+LVS
Sbjct: 245 GRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLDGLKTVELACRSQCGINDLVS 304
Query: 863 TFITLLTEENPARERTIVRTAEKLVPFQFNRINESLDS----GDRRRRRKVNLKSYESIS 1030
+F+ LL EENP+RE TIVRTA KL PF FN+I E+ DS +RR ++ NLK S++
Sbjct: 305 SFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDSNVPMATQRRLKRFNLKYDGSMT 364
Query: 1031 SEFLCPICCSPVNNLNLEYLKSKLESCATSPTVFGDSCCPSCQFQILPKESSSMEHFYSL 1210
+E CPIC P+N + L + E S ++ +CC SC+FQILP++ SS+E F S
Sbjct: 365 TEAFCPICNGPLNRSDSSELDT-FEEGQESDVLYA-ACCSSCRFQILPQDGSSLEQFSSF 422
Query: 1211 VPQQMITRVKDSVGGDQAWLREQIEDCLLSDGEDEV 1318
+P MI++VK QA+LRE+I+DCLL D E+ V
Sbjct: 423 LPDHMISQVKHQKVDSQAYLREKIKDCLLLDDEEVV 458