BLASTX nr result
ID: Coptis23_contig00002444
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00002444
(1941 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 828 0.0
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 820 0.0
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 712 0.0
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 634 e-179
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 634 e-179
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 828 bits (2140), Expect = 0.0
Identities = 426/488 (87%), Positives = 431/488 (88%)
Frame = -1
Query: 1773 MEVVSIAIVSXXXXXXXXXXLRDSRQKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 1594
MEVVSIAIVS LRD RQKNLPPGPRP PIVGNLLQLGDKP AEFAKLAQKY
Sbjct: 1 MEVVSIAIVSFSFLLFLFFILRDPRQKNLPPGPRPLPIVGNLLQLGDKPRAEFAKLAQKY 60
Query: 1593 GQLFSLKLGSQTXXXXXXXXXXXXVLKTHDRILSGRYVFQSFRVKEHVENSIVWSECNDN 1414
GQLFSLKLGSQT VLKTHD+ILSGRYVFQSFRVKEHVENSIVWSECNDN
Sbjct: 61 GQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120
Query: 1413 WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRSXXXXXXXXXXXVFGTLVNIFGN 1234
WKLLRKVCRTELFTPKMIESQSEIRE KAREMVKFLRS VFGTLVNIFGN
Sbjct: 121 WKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGN 180
Query: 1233 LIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVAEVLQ 1054
LIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVA+VLQ
Sbjct: 181 LIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQ 240
Query: 1053 QIYDVWGINLKERRGMKGSESNNDFVNVLLNAGLEDQKINALLMELFGAGTETSASTIEW 874
QIYDVWGINLKERRGMKGSESNNDFV+VLLNAGLEDQKINALLMELFG GTETSASTIEW
Sbjct: 241 QIYDVWGINLKERRGMKGSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEW 300
Query: 873 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXX 694
AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKE
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRR 360
Query: 693 XXXTCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 514
TCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF
Sbjct: 361 ALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 420
Query: 513 GGGRRICPGLPLASQFINLIVATLVQNFEWSLPQGMSTSELSMNEKFGLTLQKDPPLLIV 334
GGGRRICPGLPLASQFI+LIVATLVQNFEWSLPQGMSTSELSM+EKFGLTLQKDPPLLIV
Sbjct: 421 GGGRRICPGLPLASQFISLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIV 480
Query: 333 LKARPSNI 310
LKARPS I
Sbjct: 481 LKARPSYI 488
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 820 bits (2118), Expect = 0.0
Identities = 420/488 (86%), Positives = 429/488 (87%)
Frame = -1
Query: 1773 MEVVSIAIVSXXXXXXXXXXLRDSRQKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 1594
MEV+SIAIVS LRDSR KNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY
Sbjct: 1 MEVLSIAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 60
Query: 1593 GQLFSLKLGSQTXXXXXXXXXXXXVLKTHDRILSGRYVFQSFRVKEHVENSIVWSECNDN 1414
G+LFSLKLGSQT +LKTHD+ILSGRYVFQSFRVKEHVENSIVWSECNDN
Sbjct: 61 GELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120
Query: 1413 WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRSXXXXXXXXXXXVFGTLVNIFGN 1234
WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLR VFGTLVNIFGN
Sbjct: 121 WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGN 180
Query: 1233 LIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVAEVLQ 1054
LIFSKDVFDLEDPTGGSVELKEHLWKLL+MGNSTNPADYFPIMGK DLFGQRRAVAEVLQ
Sbjct: 181 LIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQ 240
Query: 1053 QIYDVWGINLKERRGMKGSESNNDFVNVLLNAGLEDQKINALLMELFGAGTETSASTIEW 874
QIYDVWG+ LKERRG KGSES NDFV+VLLNAGL+DQKINALLMELFGAGTETSASTIEW
Sbjct: 241 QIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW 300
Query: 873 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXX 694
AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKE
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRR 360
Query: 693 XXXTCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 514
TCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF
Sbjct: 361 ALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 420
Query: 513 GGGRRICPGLPLASQFINLIVATLVQNFEWSLPQGMSTSELSMNEKFGLTLQKDPPLLIV 334
GGGRRICPGLPLASQF NLIVATLVQNFEWSLPQGMSTSELSM+EKFGLTLQKDPPLLIV
Sbjct: 421 GGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIV 480
Query: 333 LKARPSNI 310
LKAR SNI
Sbjct: 481 LKARASNI 488
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 712 bits (1837), Expect = 0.0
Identities = 362/487 (74%), Positives = 399/487 (81%), Gaps = 1/487 (0%)
Frame = -1
Query: 1773 MEVVSIAIVSXXXXXXXXXXLR-DSRQKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQK 1597
MEV+S A+VS + DSR K+LPPGPRPSPIVGNLLQLG+KPHAEFAKLA+K
Sbjct: 1 MEVLSAAMVSLFFIFLFIFFILCDSRNKDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEK 60
Query: 1596 YGQLFSLKLGSQTXXXXXXXXXXXXVLKTHDRILSGRYVFQSFRVKEHVENSIVWSECND 1417
YG+LF+LKLGSQT +LKT D+ILSGRYVFQSFRV EHV NSIVWSECN+
Sbjct: 61 YGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNE 120
Query: 1416 NWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRSXXXXXXXXXXXVFGTLVNIFG 1237
NWKLLRKVCRTELF+PKMIESQ+ IREAKA +MV+FLR VF TLVNIFG
Sbjct: 121 NWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFG 180
Query: 1236 NLIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVAEVL 1057
NLIFSKDVFDLEDPTGGS ELKEHLWKLL+MGNSTNPADYFPIMGK+DLFGQRR VA+VL
Sbjct: 181 NLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVL 240
Query: 1056 QQIYDVWGINLKERRGMKGSESNNDFVNVLLNAGLEDQKINALLMELFGAGTETSASTIE 877
+QIYDVWG+ LKERR + G NDF NVLLNAGL+DQKINALLMELFGAGTET+ASTIE
Sbjct: 241 KQIYDVWGVMLKERRSITG-HRENDFANVLLNAGLDDQKINALLMELFGAGTETTASTIE 299
Query: 876 WAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXX 697
WAITELT V+SK+R EL+NVVG TVKES++PHLPYLQA VKE
Sbjct: 300 WAITELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKETLRLHPPTPLLLPR 359
Query: 696 XXXXTCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIP 517
TC VMNYTIPKECQIMVNAWAIGRDPKTWDDPL FKPERF++S VDYKGNDFELIP
Sbjct: 360 RALETCKVMNYTIPKECQIMVNAWAIGRDPKTWDDPLTFKPERFMNSTVDYKGNDFELIP 419
Query: 516 FGGGRRICPGLPLASQFINLIVATLVQNFEWSLPQGMSTSELSMNEKFGLTLQKDPPLLI 337
FGGGRRICPGLPLASQF++LIVATLVQNF+WS PQGM+ +E+ M+EKFGL LQKDPPLLI
Sbjct: 420 FGGGRRICPGLPLASQFLSLIVATLVQNFDWSFPQGMTPNEVPMDEKFGLPLQKDPPLLI 479
Query: 336 VLKARPS 316
VLKAR S
Sbjct: 480 VLKARTS 486
>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 634 bits (1636), Expect = e-179
Identities = 310/465 (66%), Positives = 368/465 (79%), Gaps = 2/465 (0%)
Frame = -1
Query: 1710 RDSRQKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGQLFSLKLGSQTXXXXXXXXX 1531
RDS K LPPGP+P PIVGNLLQLG+KPH++FA+LA+ YG LFSLKLGS+T
Sbjct: 16 RDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLA 75
Query: 1530 XXXVLKTHDRILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQ 1351
+LKTHDR+LSGRYVFQSFRVKEHVENSIVWSECN+ WK LRKVCRTELFT KMIESQ
Sbjct: 76 ASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQ 135
Query: 1350 SEIREAKAREMVKFLRSXXXXXXXXXXXVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELK 1171
+E+RE+KA EMV++L+ VFGTLVNIFGNLIFS+++F L D + GSVE+K
Sbjct: 136 AEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMK 195
Query: 1170 EHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVAEVLQQIYDVWGINLKERRGMK--GS 997
EHLW++LE+GNSTNPADYFP +GK+DLFGQ + VA+ LQ IY VWG LKER+ K +
Sbjct: 196 EHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNN 255
Query: 996 ESNNDFVNVLLNAGLEDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLEL 817
NDFV +LL++GL+DQ+INALLME+FGAGTETSASTIEWA++ELTK P V + +RLEL
Sbjct: 256 SKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLEL 315
Query: 816 VNVVGDNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXXXXXTCTVMNYTIPKECQIM 637
++VVG VKESD+P++PYLQAFVKE TC V+NYTIPKECQIM
Sbjct: 316 LSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIM 375
Query: 636 VNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFINL 457
VNAW IGRDPK W DPL F PERFL+S +D+KGNDFELIPFG GRRICPG+PLA+QFI+L
Sbjct: 376 VNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISL 435
Query: 456 IVATLVQNFEWSLPQGMSTSELSMNEKFGLTLQKDPPLLIVLKAR 322
IV++LVQNF+W P+GM S+L M EKFGLTLQK+PPL IV K R
Sbjct: 436 IVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 634 bits (1634), Expect = e-179
Identities = 313/483 (64%), Positives = 374/483 (77%), Gaps = 2/483 (0%)
Frame = -1
Query: 1764 VSIAIVSXXXXXXXXXXLRDSRQKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGQL 1585
V++++V+ RDS K LPPGP+P PIVGNLLQLG+KPH++FA+LA+ YG L
Sbjct: 3 VTVSLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDL 62
Query: 1584 FSLKLGSQTXXXXXXXXXXXXVLKTHDRILSGRYVFQSFRVKEHVENSIVWSECNDNWKL 1405
F+LKLGS+T +LKTHDR+LSGRYVFQSFRVKEHVENSIVWSECN+ WK
Sbjct: 63 FTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKK 122
Query: 1404 LRKVCRTELFTPKMIESQSEIREAKAREMVKFLRSXXXXXXXXXXXVFGTLVNIFGNLIF 1225
LRKVCR ELFT KMIESQ+EIRE+KA EMV+FL+ VFGTLVNIFGNLIF
Sbjct: 123 LRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIF 182
Query: 1224 SKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVAEVLQQIY 1045
S+++F L D + GSVE+KEHLW++LE+GNSTNPADYFP +G++DLFGQR+ VA+ LQ IY
Sbjct: 183 SQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIY 242
Query: 1044 DVWGINLKERRGMK--GSESNNDFVNVLLNAGLEDQKINALLMELFGAGTETSASTIEWA 871
VWG LKER+ K + NDFV +LL++GL+DQ+INALLME+FGAGTETSASTIEWA
Sbjct: 243 SVWGAMLKERKIAKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWA 302
Query: 870 ITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXXX 691
++ELTK P V + +R EL++VVG VKESD+P++PYLQAFVKE
Sbjct: 303 LSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRA 362
Query: 690 XXTCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFG 511
TC V+NYTIPKECQIMVNAW IGRDPK W DPL F PERFL+S +D+KGNDFELIPFG
Sbjct: 363 LETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFAPERFLNSSIDFKGNDFELIPFG 422
Query: 510 GGRRICPGLPLASQFINLIVATLVQNFEWSLPQGMSTSELSMNEKFGLTLQKDPPLLIVL 331
GRRICPG+PLA+QFI+LIV TLVQNF+W LP+GM S+L M EKFGLTLQK+PPL IV
Sbjct: 423 AGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVP 482
Query: 330 KAR 322
K R
Sbjct: 483 KNR 485