BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00002337
         (838 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|A2A1A1.2|NCS2_COPJA RecName: Full=S-norcoclaurine synthase 2;...   262   3e-84
sp|Q67A25.1|NCS_THLFG RecName: Full=S-norcoclaurine synthase; Fl...   183   4e-51
gb|ACO90247.1| norcoclaurine synthase [Thalictrum flavum]             184   3e-44
pdb|2VNE|A Chain A, The X-Ray Structure Of Norcoclaurine Synthas...   174   3e-41
gb|ACJ76785.1| S-norcoclaurine synthase 1 [Argemone mexicana]         129   6e-28

>sp|A2A1A1.2|NCS2_COPJA RecName: Full=S-norcoclaurine synthase 2; AltName:
           Full=Pathogenesis related protein 10A; Short=CjPR10A;
           Flags: Precursor gi|289176978|dbj|BAF45338.2|
           pathogenesis related protein 10A [Coptis japonica var.
           dissecta]
          Length = 196

 Score =  262 bits (669), Expect(2) = 3e-84
 Identities = 125/129 (96%), Positives = 128/129 (99%)
 Frame = -3

Query: 617 LHLPDLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEMID 438
           LHLPDLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVE ID
Sbjct: 65  LHLPDLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEQID 124

Query: 437 GDFFDLGVTYYMDTIRVVATGPDSCVIKSSTEYHVKPEYAKIVKPLIDTVPLAIMSEAIA 258
           GDFFDLGVTYYMDTIRVVATGPDSCVIKS+TEYHVKPE+AKIVKPLIDTVPLAIMSEAIA
Sbjct: 125 GDFFDLGVTYYMDTIRVVATGPDSCVIKSTTEYHVKPEFAKIVKPLIDTVPLAIMSEAIA 184

Query: 257 KVVLENRHK 231
           KVVLEN+HK
Sbjct: 185 KVVLENKHK 193



 Score = 77.0 bits (188), Expect(2) = 3e-84
 Identities = 37/38 (97%), Positives = 37/38 (97%)
 Frame = -1

Query: 766 MRMEVVLVVFLMFIGTINCERLIFNGRPLLHRVTNEET 653
           MRMEVVLVVFLMFIGTINCERLIFNGRPLLHRVT EET
Sbjct: 1   MRMEVVLVVFLMFIGTINCERLIFNGRPLLHRVTKEET 38


>sp|Q67A25.1|NCS_THLFG RecName: Full=S-norcoclaurine synthase; Flags: Precursor
           gi|38492394|gb|AAR22502.1| (S)-norcoclaurine synthase
           [Thalictrum flavum subsp. glaucum]
           gi|226897754|gb|ACO90248.1| norcoclaurine synthase
           [Thalictrum flavum]
          Length = 210

 Score =  183 bits (464), Expect(2) = 4e-51
 Identities = 88/128 (68%), Positives = 106/128 (82%)
 Frame = -3

Query: 614 HLPDLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEMIDG 435
           HLPDLLP G F K EI GDGG G+ILDMTF PG+FPH Y+EKF+  D+++R K V+MI+G
Sbjct: 71  HLPDLLP-GAFEKLEIIGDGGVGTILDMTFVPGEFPHEYKEKFILVDNEHRLKKVQMIEG 129

Query: 434 DFFDLGVTYYMDTIRVVATGPDSCVIKSSTEYHVKPEYAKIVKPLIDTVPLAIMSEAIAK 255
            + DLGVTYYMDTI VV TG DSCVIKSSTEYHVKPE+ KIV+PLI T PLA M++AI+K
Sbjct: 130 GYLDLGVTYYMDTIHVVPTGKDSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAMADAISK 189

Query: 254 VVLENRHK 231
           +VLE++ K
Sbjct: 190 LVLEHKSK 197



 Score = 45.4 bits (106), Expect(2) = 4e-51
 Identities = 21/31 (67%), Positives = 25/31 (80%)
 Frame = -1

Query: 766 MRMEVVLVVFLMFIGTINCERLIFNGRPLLH 674
           M+MEVV V FLM +GTINC++LI  GRP LH
Sbjct: 2   MKMEVVFV-FLMLLGTINCQKLILTGRPFLH 31


>gb|ACO90247.1| norcoclaurine synthase [Thalictrum flavum]
          Length = 190

 Score =  184 bits (466), Expect = 3e-44
 Identities = 87/128 (67%), Positives = 108/128 (84%)
 Frame = -3

Query: 614 HLPDLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEMIDG 435
           HLPDLLP G F K EI GDGG G+ILDMTF PG+FPH Y+EKF+  D+++R K V+MI+G
Sbjct: 45  HLPDLLP-GAFEKLEIIGDGGVGTILDMTFTPGEFPHEYKEKFILVDNEHRLKKVQMIEG 103

Query: 434 DFFDLGVTYYMDTIRVVATGPDSCVIKSSTEYHVKPEYAKIVKPLIDTVPLAIMSEAIAK 255
            + DLGVTYYMDTI+V+ TG +SCVIKSSTEYHVKPE+ KIV+PLI T PLA M+EAI+K
Sbjct: 104 GYLDLGVTYYMDTIQVIPTGTNSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAMAEAISK 163

Query: 254 VVLENRHK 231
           +VLE+++K
Sbjct: 164 LVLEHKYK 171


>pdb|2VNE|A Chain A, The X-Ray Structure Of Norcoclaurine Synthase From
           Thalictrum Flavum gi|195927323|pdb|2VNE|B Chain B, The
           X-Ray Structure Of Norcoclaurine Synthase From
           Thalictrum Flavum gi|195927324|pdb|2VQ5|A Chain A, X-Ray
           Structure Of Norcoclaurine Synthase From Thalictrum
           Flavum In Complex With Dopamine And Hydroxybenzaldehyde
           gi|195927325|pdb|2VQ5|B Chain B, X-Ray Structure Of
           Norcoclaurine Synthase From Thalictrum Flavum In Complex
           With Dopamine And Hydroxybenzaldehyde
          Length = 201

 Score =  174 bits (440), Expect = 3e-41
 Identities = 84/128 (65%), Positives = 102/128 (79%)
 Frame = -3

Query: 614 HLPDLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEMIDG 435
           HLPDLLP G F K EI GDGG G+ILD TF PG+FPH Y+EKF+  D+++R K V+ I+G
Sbjct: 56  HLPDLLP-GAFEKLEIIGDGGVGTILDXTFVPGEFPHEYKEKFILVDNEHRLKKVQXIEG 114

Query: 434 DFFDLGVTYYMDTIRVVATGPDSCVIKSSTEYHVKPEYAKIVKPLIDTVPLAIMSEAIAK 255
            + DLGVTYY DTI VV TG DSCVIKSSTEYHVKPE+ KIV+PLI T PLA  ++AI+K
Sbjct: 115 GYLDLGVTYYXDTIHVVPTGKDSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAXADAISK 174

Query: 254 VVLENRHK 231
           +VLE++ K
Sbjct: 175 LVLEHKSK 182


>gb|ACJ76785.1| S-norcoclaurine synthase 1 [Argemone mexicana]
          Length = 275

 Score =  129 bits (325), Expect = 6e-28
 Identities = 66/126 (52%), Positives = 86/126 (68%), Gaps = 1/126 (0%)
 Frame = -3

Query: 611 LPDLLPAGIFAKFE-ITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEMIDG 435
           L D+L  G+F K + I G+GG G++LD+ FPPG  P  Y+EKF+  D+K R K V MI+G
Sbjct: 58  LRDVLLPGVFEKLDVIEGNGGVGTVLDIVFPPGAVPRCYKEKFINIDNKKRLKEVIMIEG 117

Query: 434 DFFDLGVTYYMDTIRVVATGPDSCVIKSSTEYHVKPEYAKIVKPLIDTVPLAIMSEAIAK 255
              D+G TYY+D I V+A  P+SCVIKSS  Y VK EYA+ +  LI T+PL  MSE IA 
Sbjct: 118 GHLDMGCTYYLDRIHVIAKTPNSCVIKSSIIYDVKKEYAEAMSKLITTIPLKSMSEVIAN 177

Query: 254 VVLENR 237
            VL+N+
Sbjct: 178 YVLKNQ 183