BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00002335
         (2500 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278061.1| PREDICTED: probable pectinesterase/pectinest...   426   e-116
ref|XP_002321999.1| predicted protein [Populus trichocarpa] gi|2...   423   e-115
emb|CAE76634.1| pectin methylesterase [Cicer arietinum]               422   e-115
emb|CBI23005.3| unnamed protein product [Vitis vinifera]              417   e-114
ref|XP_003602238.1| Pectinesterase [Medicago truncatula] gi|3554...   416   e-113

>ref|XP_002278061.1| PREDICTED: probable pectinesterase/pectinesterase inhibitor 34-like
            [Vitis vinifera]
          Length = 597

 Score =  426 bits (1095), Expect = e-116
 Identities = 220/317 (69%), Positives = 261/317 (82%), Gaps = 4/317 (1%)
 Frame = +2

Query: 179  SAILLVHYRTNGSGPSESLVRAKPTQAISKTCSKTRYPELCVNSLLDFPGSLTADQRDLV 358
            S + LV  R   +  S+  VR KPTQAIS TCS+TR+P LCV+SLLDFPGSLTA +RDLV
Sbjct: 49   STVALVVRREGSAAGSDGRVRRKPTQAISHTCSRTRFPTLCVDSLLDFPGSLTAGERDLV 108

Query: 359  HISVNMTLQRVGHALYGASEISNLEMDTRVRSAYEDCLELLEDSVDHLSSSLNSV--GPD 532
            HIS+NMTLQR G ALY +SEI+NL+MDTRVR+AYEDCLELLE+SV+ LS SL SV  G D
Sbjct: 109  HISMNMTLQRFGKALYVSSEIANLQMDTRVRAAYEDCLELLEESVEQLSRSLTSVAGGGD 168

Query: 533  SKA-GSTGDVLTWLSAALTNQDTCTEGFEDVNGVVKNQMVLQLRDLTELVSNCLAIYSAS 709
             +A GST DVLTWLSAA+TNQDTCTEGF+DV+G VK+QMV +LRDL++LVSNCLAI++AS
Sbjct: 169  GQAVGSTQDVLTWLSAAMTNQDTCTEGFDDVSGFVKDQMVEKLRDLSDLVSNCLAIFAAS 228

Query: 710  SDDEDFSGIPIQNRRKLLEYDP-VPTYTTLPPFPPWVGRKDRRLLQMPISAMQADIIVSK 886
              D DF+G+PIQNRR+ L  D  +        FP W+ R++R LLQMP+ A+QADIIVS+
Sbjct: 229  GGDNDFAGVPIQNRRRRLMQDSDISANQDSTGFPKWLTRRERSLLQMPVPAIQADIIVSQ 288

Query: 887  DGNGTYKTITEAIKAAPQKSSRRTVIYVKAGRYEEKMIKVGKKNTNLMFIGDGMGKTIIS 1066
            DGNGTYKTITEAIK AP+ SSRRT+IYVKAGRYEE  +KVG+K TNLMFIGDG GKTII+
Sbjct: 289  DGNGTYKTITEAIKKAPEYSSRRTIIYVKAGRYEENNLKVGRKKTNLMFIGDGKGKTIIT 348

Query: 1067 GGKNVFDGLTTFHTASF 1117
            GGK+VF+ LTTFHTASF
Sbjct: 349  GGKSVFNNLTTFHTASF 365



 Score =  417 bits (1073), Expect = e-114
 Identities = 195/238 (81%), Positives = 208/238 (87%)
 Frame = +2

Query: 1511 FHTXXXXXXXXXXIARDMTFENWAGPSKHQAVALRVGADHAVVYRCNVIGYQDTLYVHSQ 1690
            FHT          IARDMTFENWAGP KHQAVALRVGADH VVYRCN+IGYQDTLYVHSQ
Sbjct: 360  FHTASFAATGAGFIARDMTFENWAGPGKHQAVALRVGADHGVVYRCNIIGYQDTLYVHSQ 419

Query: 1691 RQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKPMAQQMNTITAQSRKDPNQNTGISIHN 1870
            RQF+RECDIYGTVDFIFGNAAVVFQNCS+YARKPMAQQ NTITAQ+RKDPNQNTGISIH 
Sbjct: 420  RQFFRECDIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHA 479

Query: 1871 CRIVATSDLEASKNSFATYLGRPWKMYSRVVYMISYMGGHIQPQGWLEWNSTSALDKLYY 2050
            CRI+   DL   K SF TYLGRPWK+YSR VYM+SYMG HI P+GWLEWN+T ALD LYY
Sbjct: 480  CRILPAGDLAPVKGSFPTYLGRPWKLYSRTVYMLSYMGDHIHPKGWLEWNTTFALDTLYY 539

Query: 2051 GEYMNYGPGGAVGQRVKWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFVSGLSL 2224
            GEYMNYGPGGAVGQRV WPGYRVITS VEA+KFTV QFIYGSSWLPSTGVAF++GLS+
Sbjct: 540  GEYMNYGPGGAVGQRVNWPGYRVITSVVEATKFTVGQFIYGSSWLPSTGVAFLAGLSV 597


>ref|XP_002321999.1| predicted protein [Populus trichocarpa] gi|222868995|gb|EEF06126.1|
            predicted protein [Populus trichocarpa]
          Length = 605

 Score =  423 bits (1087), Expect = e-115
 Identities = 197/238 (82%), Positives = 214/238 (89%)
 Frame = +2

Query: 1511 FHTXXXXXXXXXXIARDMTFENWAGPSKHQAVALRVGADHAVVYRCNVIGYQDTLYVHSQ 1690
            FHT          IARDMTFENWAGP+KHQAVALRVGADHAVVYRCN+IGYQDTLYVHS 
Sbjct: 368  FHTASFAATGAGFIARDMTFENWAGPAKHQAVALRVGADHAVVYRCNIIGYQDTLYVHSN 427

Query: 1691 RQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKPMAQQMNTITAQSRKDPNQNTGISIHN 1870
            RQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKPMA Q NTITAQ+RKDPNQNTGISIH 
Sbjct: 428  RQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKPMAFQKNTITAQNRKDPNQNTGISIHA 487

Query: 1871 CRIVATSDLEASKNSFATYLGRPWKMYSRVVYMISYMGGHIQPQGWLEWNSTSALDKLYY 2050
            CRI+ATSDL   K SF T+LGRPWK+YSR VYM+SY+G H+ P+GWLEWN+T ALD LYY
Sbjct: 488  CRILATSDLTPLKGSFPTFLGRPWKLYSRTVYMLSYIGDHVHPRGWLEWNTTFALDTLYY 547

Query: 2051 GEYMNYGPGGAVGQRVKWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFVSGLSL 2224
            GEYMNYGPGGAVGQRVKWPGYRV+TST+EASKFTVAQFIYGSSWLPSTGV+F++GLSL
Sbjct: 548  GEYMNYGPGGAVGQRVKWPGYRVVTSTIEASKFTVAQFIYGSSWLPSTGVSFLAGLSL 605



 Score =  398 bits (1023), Expect = e-108
 Identities = 207/321 (64%), Positives = 255/321 (79%), Gaps = 6/321 (1%)
 Frame = +2

Query: 173  AISAILLVHYRTNGSGPSE--SLVRAKPTQAISKTCSKTRYPELCVNSLLDFPGSLTADQ 346
            A+SA+LL+  RT  SG  +  SL   KPTQAISKTCSKTR+P LCV+SLLDFPGS++A +
Sbjct: 56   AVSAVLLLGIRTKASGQPDPSSLTHRKPTQAISKTCSKTRFPNLCVSSLLDFPGSVSASE 115

Query: 347  RDLVHISVNMTLQRVGHALYGASEISNLEMDTRVRSAYEDCLELLEDSVDHLSSSLNSVG 526
             DLVHIS NMTLQ    ALY +S IS + M+TRVRSA++DCLELL+DS+D LS SL++V 
Sbjct: 116  SDLVHISFNMTLQHFSKALYLSSAISYVNMETRVRSAFDDCLELLDDSIDALSRSLSTVA 175

Query: 527  PDSKAGSTG----DVLTWLSAALTNQDTCTEGFEDVNGVVKNQMVLQLRDLTELVSNCLA 694
            P    G+ G    DV+TWLSAALTNQDTC+EGF+ VNG VKNQM  +L+DLTELVSNCLA
Sbjct: 176  PSHGGGTGGGSPADVVTWLSAALTNQDTCSEGFDGVNGAVKNQMTGRLKDLTELVSNCLA 235

Query: 695  IYSASSDDEDFSGIPIQNRRKLLEYDPVPTYTTLPPFPPWVGRKDRRLLQMPISAMQADI 874
            I+S+++ D DFSG+P+QN+R+LL  +   +Y     FP W+GR+DR+LL +P+ A+ ADI
Sbjct: 236  IFSSANGD-DFSGVPVQNKRRLLTENEDISYEE--NFPRWLGRRDRKLLDVPVPAIHADI 292

Query: 875  IVSKDGNGTYKTITEAIKAAPQKSSRRTVIYVKAGRYEEKMIKVGKKNTNLMFIGDGMGK 1054
            IVS DGNGT KTI+EAIK AP+ S+RRTVIYV+AGRYEE  +KVG+K  NLMFIGDG GK
Sbjct: 293  IVSGDGNGTCKTISEAIKKAPEYSTRRTVIYVRAGRYEENNLKVGRKKWNLMFIGDGKGK 352

Query: 1055 TIISGGKNVFDGLTTFHTASF 1117
            TIISGGK+V + LTTFHTASF
Sbjct: 353  TIISGGKSVLNNLTTFHTASF 373


>emb|CAE76634.1| pectin methylesterase [Cicer arietinum]
          Length = 254

 Score =  422 bits (1086), Expect = e-115
 Identities = 197/237 (83%), Positives = 215/237 (90%)
 Frame = +2

Query: 1511 FHTXXXXXXXXXXIARDMTFENWAGPSKHQAVALRVGADHAVVYRCNVIGYQDTLYVHSQ 1690
            FHT          IARD+TFEN+AGP+KHQAVALRVGADHAVVYRCN+IGYQDT+YVHS 
Sbjct: 17   FHTASFAASGPGFIARDVTFENYAGPAKHQAVALRVGADHAVVYRCNIIGYQDTMYVHSN 76

Query: 1691 RQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKPMAQQMNTITAQSRKDPNQNTGISIHN 1870
            RQFYRECDIYGTVDFIFGNAAVVFQNCS+YARKPMAQQ NTITAQ+RKDPNQNTGISIHN
Sbjct: 77   RQFYRECDIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHN 136

Query: 1871 CRIVATSDLEASKNSFATYLGRPWKMYSRVVYMISYMGGHIQPQGWLEWNSTSALDKLYY 2050
            CRI+AT DLEASK SF TYLGRPWK+YS+ VYM+SYMG HI P+GWLEWN+T ALD LYY
Sbjct: 137  CRILATPDLEASKGSFQTYLGRPWKLYSKTVYMLSYMGDHIHPRGWLEWNATFALDTLYY 196

Query: 2051 GEYMNYGPGGAVGQRVKWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFVSGLS 2221
            GEYMNYGPGGA+GQRV WPG+RVITSTVEA++FTVAQFI GSSWLPSTGVAFV+GLS
Sbjct: 197  GEYMNYGPGGAIGQRVTWPGFRVITSTVEANRFTVAQFISGSSWLPSTGVAFVAGLS 253


>emb|CBI23005.3| unnamed protein product [Vitis vinifera]
          Length = 393

 Score =  417 bits (1072), Expect = e-114
 Identities = 195/237 (82%), Positives = 207/237 (87%)
 Frame = +2

Query: 1511 FHTXXXXXXXXXXIARDMTFENWAGPSKHQAVALRVGADHAVVYRCNVIGYQDTLYVHSQ 1690
            FHT          IARDMTFENWAGP KHQAVALRVGADH VVYRCN+IGYQDTLYVHSQ
Sbjct: 114  FHTASFAATGAGFIARDMTFENWAGPGKHQAVALRVGADHGVVYRCNIIGYQDTLYVHSQ 173

Query: 1691 RQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKPMAQQMNTITAQSRKDPNQNTGISIHN 1870
            RQF+RECDIYGTVDFIFGNAAVVFQNCS+YARKPMAQQ NTITAQ+RKDPNQNTGISIH 
Sbjct: 174  RQFFRECDIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHA 233

Query: 1871 CRIVATSDLEASKNSFATYLGRPWKMYSRVVYMISYMGGHIQPQGWLEWNSTSALDKLYY 2050
            CRI+   DL   K SF TYLGRPWK+YSR VYM+SYMG HI P+GWLEWN+T ALD LYY
Sbjct: 234  CRILPAGDLAPVKGSFPTYLGRPWKLYSRTVYMLSYMGDHIHPKGWLEWNTTFALDTLYY 293

Query: 2051 GEYMNYGPGGAVGQRVKWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFVSGLS 2221
            GEYMNYGPGGAVGQRV WPGYRVITS VEA+KFTV QFIYGSSWLPSTGVAF++GLS
Sbjct: 294  GEYMNYGPGGAVGQRVNWPGYRVITSVVEATKFTVGQFIYGSSWLPSTGVAFLAGLS 350



 Score =  170 bits (430), Expect = 2e-39
 Identities = 81/105 (77%), Positives = 94/105 (89%)
 Frame = +2

Query: 803  FPPWVGRKDRRLLQMPISAMQADIIVSKDGNGTYKTITEAIKAAPQKSSRRTVIYVKAGR 982
            FP W+ R++R LLQMP+ A+QADIIVS+DGNGTYKTITEAIK AP+ SSRRT+IYVKAGR
Sbjct: 15   FPKWLTRRERSLLQMPVPAIQADIIVSQDGNGTYKTITEAIKKAPEYSSRRTIIYVKAGR 74

Query: 983  YEEKMIKVGKKNTNLMFIGDGMGKTIISGGKNVFDGLTTFHTASF 1117
            YEE  +KVG+K TNLMFIGDG GKTII+GGK+VF+ LTTFHTASF
Sbjct: 75   YEENNLKVGRKKTNLMFIGDGKGKTIITGGKSVFNNLTTFHTASF 119


>ref|XP_003602238.1| Pectinesterase [Medicago truncatula] gi|355491286|gb|AES72489.1|
            Pectinesterase [Medicago truncatula]
          Length = 599

 Score =  416 bits (1069), Expect = e-113
 Identities = 192/237 (81%), Positives = 212/237 (89%)
 Frame = +2

Query: 1511 FHTXXXXXXXXXXIARDMTFENWAGPSKHQAVALRVGADHAVVYRCNVIGYQDTLYVHSQ 1690
            FHT          IA+DMTFEN+AGP+KHQAVALRV +DHAVVYRCNVIGYQDT+Y HS 
Sbjct: 362  FHTASFAASGPGFIAKDMTFENYAGPAKHQAVALRVSSDHAVVYRCNVIGYQDTMYAHSN 421

Query: 1691 RQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKPMAQQMNTITAQSRKDPNQNTGISIHN 1870
            RQFYRECDIYGTVDFIFGNAAVVFQNCS+YARKPM  Q NTITAQ+RKDPNQNTGISIHN
Sbjct: 422  RQFYRECDIYGTVDFIFGNAAVVFQNCSLYARKPMPYQKNTITAQNRKDPNQNTGISIHN 481

Query: 1871 CRIVATSDLEASKNSFATYLGRPWKMYSRVVYMISYMGGHIQPQGWLEWNSTSALDKLYY 2050
            CRI+AT DLEASK +F TYLGRPWK+YSR VYM+SYMG H+ P+GWLEWN+T ALD LYY
Sbjct: 482  CRILATQDLEASKGNFTTYLGRPWKLYSRTVYMLSYMGDHVHPRGWLEWNTTFALDTLYY 541

Query: 2051 GEYMNYGPGGAVGQRVKWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFVSGLS 2221
            GEYMNYGPGGA+GQRVKWPGYRVITSTVEA++FTVAQFI GS+WLPSTGVA+V+GLS
Sbjct: 542  GEYMNYGPGGAIGQRVKWPGYRVITSTVEANRFTVAQFISGSTWLPSTGVAYVAGLS 598



 Score =  349 bits (895), Expect = 2e-93
 Identities = 189/347 (54%), Positives = 239/347 (68%), Gaps = 12/347 (3%)
 Frame = +2

Query: 113  SKKRKXXXXXXXXXXXXXXCAISAILL--VHYRTNGSGPSESLVRAKPTQAISKTCSKTR 286
            S K+K                ISA +L  +H  T  S P    +R  PTQAIS TCSKTR
Sbjct: 28   SSKKKIIFLSLLAVLLIIASTISAAMLTGIHSHTT-SEPKNPTLRRNPTQAISNTCSKTR 86

Query: 287  YPELCVNSLLDFPGSLTADQRDLVHISVNMTLQRVGHALYGASEISN-LEMDTRVRSAYE 463
            +P LC+N LLDFP S  A ++DLVHIS+NMTLQ +  ALY ++ IS+ + ++  +R+AY 
Sbjct: 87   FPSLCINYLLDFPDSTGASEKDLVHISLNMTLQHLSKALYTSASISSTVGINPYIRAAYT 146

Query: 464  DCLELLEDSVDHLSSSLNSVGPDSKAG---------STGDVLTWLSAALTNQDTCTEGFE 616
            DCLELL++SVD L+ +L S  P S +          ST DVLTWLSAALTNQDTC EGF 
Sbjct: 147  DCLELLDNSVDALARALTSAVPSSSSNGAVKPLTSSSTEDVLTWLSAALTNQDTCAEGFA 206

Query: 617  DVNGVVKNQMVLQLRDLTELVSNCLAIYSASSDDEDFSGIPIQNRRKLLEYDPVPTYTTL 796
            D +G VK+QM   L+DL+ELVSNCLAI+SA   D DFSG+PI NRR+L+   P P     
Sbjct: 207  DTSGDVKDQMTNNLKDLSELVSNCLAIFSAGGGD-DFSGVPIGNRRRLMTM-PEPE---- 260

Query: 797  PPFPPWVGRKDRRLLQMPISAMQADIIVSKDGNGTYKTITEAIKAAPQKSSRRTVIYVKA 976
              FP W+ R++RRLL +P++ +QAD+IVSKDGNGT KTI+EA+K  P+  +RR +IY+K 
Sbjct: 261  DDFPVWLKRRERRLLSLPVTTIQADVIVSKDGNGTVKTISEALKKIPEYGNRRFIIYIKQ 320

Query: 977  GRYEEKMIKVGKKNTNLMFIGDGMGKTIISGGKNVFDGLTTFHTASF 1117
            GRYEE  +KVG+K TN+M IGDG GKT+I+GGKNV   LTTFHTASF
Sbjct: 321  GRYEEDNLKVGRKKTNVMIIGDGKGKTVITGGKNVMQNLTTFHTASF 367