BLASTX nr result
ID: Coptis23_contig00002180
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00002180
(873 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002320506.1| f-box family protein [Populus trichocarpa] g... 58 5e-16
gb|AFK37781.1| unknown [Lotus japonicus] 47 4e-15
ref|XP_002301588.1| f-box family protein [Populus trichocarpa] g... 53 1e-13
ref|XP_003610871.1| F-box family protein [Medicago truncatula] g... 53 4e-13
ref|XP_002520393.1| ubiquitin-protein ligase, putative [Ricinus ... 47 5e-13
>ref|XP_002320506.1| f-box family protein [Populus trichocarpa]
gi|222861279|gb|EEE98821.1| f-box family protein
[Populus trichocarpa]
Length = 422
Score = 58.2 bits (139), Expect(3) = 5e-16
Identities = 43/142 (30%), Positives = 65/142 (45%), Gaps = 5/142 (3%)
Frame = +1
Query: 4 PRFAQIHYQMAIQNRCSPCLGLFLDYP-RNELRLIDHQVFDNLKTSVVSSDVETYPLV-E 177
P+ ++ + Q+ PCL L D+P RN L +D + K V ++ E
Sbjct: 62 PQLVDLYLSCSTQDTDHPCLILHCDFPIRNNLYFVDFAAHEEEKEKVKRIPAPFSSMMPE 121
Query: 178 FGFSYLQIIGVVNGLVCFGEDHHRSYLPRKYIIYNPITGEHFQLPEDTCIFTDIDFAAFG 357
F +++G NGL+C + + L IYNP TG + +LP+ + FG
Sbjct: 122 F-----EVVGSCNGLLCLSDSLYNDSL----YIYNPFTGCYKELPKSLQYPDQEVVSGFG 172
Query: 358 FDSAANEYKVIRI---RDDHRG 414
F+ EYKVIRI R+ H G
Sbjct: 173 FNPKTKEYKVIRIVYYRNGHGG 194
Score = 39.7 bits (91), Expect(3) = 5e-16
Identities = 22/67 (32%), Positives = 31/67 (46%), Gaps = 13/67 (19%)
Frame = +2
Query: 686 FDEFEMWVMKDYGVQGSWAKEYKFNAQVLNCTLHNYMCGQNVFK-------------LKN 826
+ E+WVMK+Y V+ SW KEY A + N ++K LKN
Sbjct: 299 YGRLEIWVMKEYNVKESWVKEYNIGAYMPKGLKQNQDRQLKIWKNSSNGRVVGALCVLKN 358
Query: 827 GEFLVKY 847
GE L++Y
Sbjct: 359 GEILLEY 365
Score = 32.7 bits (73), Expect(3) = 5e-16
Identities = 22/68 (32%), Positives = 29/68 (42%), Gaps = 2/68 (2%)
Frame = +3
Query: 408 SRCRDFTLGTTRWRRVEDHVCRDFYFGVLGVLVNGNLHWIS--SEQDGPLSIFAFNVASE 581
S + TLG WR + V G LVNG LHWIS + +F++ E
Sbjct: 206 SEVQILTLGCPEWRSL-GKVSYRLVRRASGALVNGRLHWISRPCRNKPARRLVSFDLTDE 264
Query: 582 KFCEFESP 605
+F E P
Sbjct: 265 QFREVSKP 272
>gb|AFK37781.1| unknown [Lotus japonicus]
Length = 489
Score = 47.0 bits (110), Expect(3) = 4e-15
Identities = 28/72 (38%), Positives = 38/72 (52%), Gaps = 6/72 (8%)
Frame = +1
Query: 199 IIGVVNGLVCFGEDHHRSYLPRKYIIYNPITGEHFQLPEDTCIFTDIDF------AAFGF 360
++ NGL+C D R Y +++ NPITGE +LP+ + I F A FGF
Sbjct: 177 VVNSCNGLICLC-DRERDY----FVVCNPITGEFIRLPQTSRIGKTNKFSIQEIYAGFGF 231
Query: 361 DSAANEYKVIRI 396
NEYKV+RI
Sbjct: 232 QPKNNEYKVVRI 243
Score = 47.0 bits (110), Expect(3) = 4e-15
Identities = 28/61 (45%), Positives = 34/61 (55%)
Frame = +3
Query: 426 TLGTTRWRRVEDHVCRDFYFGVLGVLVNGNLHWISSEQDGPLSIFAFNVASEKFCEFESP 605
TLGT+ WR VE + FY V+G LHWI S G LSI F+ SE+F F +P
Sbjct: 260 TLGTSTWRNVEVNSMY-FYHLRFPTCVSGALHWIGSYH-GTLSILCFDFESERFRSFPTP 317
Query: 606 P 608
P
Sbjct: 318 P 318
Score = 33.5 bits (75), Expect(3) = 4e-15
Identities = 17/66 (25%), Positives = 29/66 (43%), Gaps = 8/66 (12%)
Frame = +2
Query: 695 FEMWVMKDYGVQGSWAKEYKFNAQV--------LNCTLHNYMCGQNVFKLKNGEFLVKYG 850
F MW+MK+YG + SW K + F+ L + ++ G + + F + Y
Sbjct: 349 FVMWIMKEYGFKESWTKIFSFDTMSSYRWPFGGLYWPVKHFKNGSAILMYHSYNFFIYYE 408
Query: 851 PKDFGY 868
P G+
Sbjct: 409 PGKDGF 414
>ref|XP_002301588.1| f-box family protein [Populus trichocarpa]
gi|222843314|gb|EEE80861.1| f-box family protein
[Populus trichocarpa]
Length = 408
Score = 52.8 bits (125), Expect(3) = 1e-13
Identities = 38/116 (32%), Positives = 53/116 (45%), Gaps = 2/116 (1%)
Frame = +1
Query: 55 PCLGLFLDYP-RNELRLIDHQVFDNLKTSVVSSDVETYPLV-EFGFSYLQIIGVVNGLVC 228
PC+ L D+P RN L +D + K V ++ EF +++G NGL+C
Sbjct: 78 PCVILHCDFPIRNNLYFVDFAAHEEEKEKVKRIRAPFSSMMPEF-----EVVGSCNGLLC 132
Query: 229 FGEDHHRSYLPRKYIIYNPITGEHFQLPEDTCIFTDIDFAAFGFDSAANEYKVIRI 396
+ L IYNP TG + +LP+ FGF+ NEYKVIRI
Sbjct: 133 LSDSLFNDSL----YIYNPFTGRYKELPKSLQYPDQEVVFGFGFNPKTNEYKVIRI 184
Score = 41.2 bits (95), Expect(3) = 1e-13
Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 13/67 (19%)
Frame = +2
Query: 686 FDEFEMWVMKDYGVQGSWAKEYKFNAQVLNCTLHNYMCGQNVFK-------------LKN 826
+ E+WVMK+Y V+ SW KEY A + N + ++K LKN
Sbjct: 298 YGRLEIWVMKEYNVKESWVKEYNIGAYMPKGLKQNLVRPLKIWKNASNGRAVRALCVLKN 357
Query: 827 GEFLVKY 847
GE L++Y
Sbjct: 358 GEILLEY 364
Score = 28.5 bits (62), Expect(3) = 1e-13
Identities = 20/68 (29%), Positives = 29/68 (42%), Gaps = 2/68 (2%)
Frame = +3
Query: 408 SRCRDFTLGTTRWRRVEDHVCRDFYFGVLGVLVNGNLHWIS--SEQDGPLSIFAFNVASE 581
S+ + TLG WR + V LVNG LHW+S + +F++ E
Sbjct: 205 SQVQILTLGCPGWRSL-GKVSYRLVRRASETLVNGRLHWVSRPCRNKPARRLVSFDLTDE 263
Query: 582 KFCEFESP 605
+F E P
Sbjct: 264 QFREVPKP 271
>ref|XP_003610871.1| F-box family protein [Medicago truncatula]
gi|355512206|gb|AES93829.1| F-box family protein
[Medicago truncatula]
Length = 503
Score = 52.8 bits (125), Expect(3) = 4e-13
Identities = 31/75 (41%), Positives = 38/75 (50%), Gaps = 3/75 (4%)
Frame = +1
Query: 184 FSYLQIIGVVNGLVCFGEDHHRSYLPRKYIIYNPITGEHFQLPEDTCIFTDI---DFAAF 354
F I+ NGL+C + Y +I NPITGE +LPE T T + AAF
Sbjct: 219 FDKFNIVNSCNGLLCLSDP----YFGNPLVICNPITGEFIRLPESTANQTRVGRQGRAAF 274
Query: 355 GFDSAANEYKVIRIR 399
GF NEYKVI +R
Sbjct: 275 GFQPKTNEYKVINMR 289
Score = 43.1 bits (100), Expect(3) = 4e-13
Identities = 26/63 (41%), Positives = 31/63 (49%)
Frame = +3
Query: 426 TLGTTRWRRVEDHVCRDFYFGVLGVLVNGNLHWISSEQDGPLSIFAFNVASEKFCEFESP 605
TLGT WR VE F+ VNG LHWI E + +SI F SE+ F SP
Sbjct: 310 TLGTPSWRNVEVDPQISFWMLKNPTCVNGALHWIRYEGE-QMSILVFCFESERLQSFPSP 368
Query: 606 PFQ 614
P +
Sbjct: 369 PHE 371
Score = 24.6 bits (52), Expect(3) = 4e-13
Identities = 7/15 (46%), Positives = 12/15 (80%)
Frame = +2
Query: 716 DYGVQGSWAKEYKFN 760
+YG++ SW K YK++
Sbjct: 385 EYGIEESWTKVYKYD 399
>ref|XP_002520393.1| ubiquitin-protein ligase, putative [Ricinus communis]
gi|223540440|gb|EEF42009.1| ubiquitin-protein ligase,
putative [Ricinus communis]
Length = 369
Score = 47.0 bits (110), Expect(3) = 5e-13
Identities = 34/132 (25%), Positives = 61/132 (46%), Gaps = 2/132 (1%)
Frame = +1
Query: 4 PRFAQIHYQMAIQNRCSPCLGLFLDYPRNELRLIDHQVFDNLKTSVVSSDVETYPLVEFG 183
P FA++H + +Q L L + + +D + + + + + + +P+
Sbjct: 43 PEFAKLHLERVLQVTNVHLHRLLLS--ADPFQSVDMEAYCDADDNFLIRE-HRFPVRNPE 99
Query: 184 FSYLQIIGVVNGLVC--FGEDHHRSYLPRKYIIYNPITGEHFQLPEDTCIFTDIDFAAFG 357
+ +G NGL+ FG DH + ++NP TGE +LP T D F FG
Sbjct: 100 DDDFEFVGSCNGLISAVFGSDH-------EITVWNPSTGESRKLPAPTSSTEDKLFYGFG 152
Query: 358 FDSAANEYKVIR 393
+DS ++YK++R
Sbjct: 153 YDSKLDDYKIVR 164
Score = 37.7 bits (86), Expect(3) = 5e-13
Identities = 19/57 (33%), Positives = 31/57 (54%)
Frame = +3
Query: 423 FTLGTTRWRRVEDHVCRDFYFGVLGVLVNGNLHWISSEQDGPLSIFAFNVASEKFCE 593
F L RWR +++ C + G + +NG LHW+ + + L I + ++A EKF E
Sbjct: 179 FNLKGNRWRAIQNLHCNVRFQGS-AIALNGILHWLVDQLNEGLMIVSLDLAEEKFLE 234
Score = 35.4 bits (80), Expect(3) = 5e-13
Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 1/70 (1%)
Frame = +2
Query: 641 LRVIEELLCFIDMKEFDEFEMWVMKDYGVQGSWAKEYKFNAQVL-NCTLHNYMCGQNVFK 817
L+V+ + L FE W +K YG + SW K + FN+ L C + ++
Sbjct: 250 LKVLGDSLSVCSSSHTTNFEAWTVKGYGSKASWLKLFSFNSDPLPGCKYWLNV----LWV 305
Query: 818 LKNGEFLVKY 847
KNG L+ Y
Sbjct: 306 AKNGNVLLNY 315