BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00001876
         (1673 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   863   0.0  
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]                    501   e-139
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN...   489   e-136
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   432   e-118
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   428   e-117

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  863 bits (2231), Expect = 0.0
 Identities = 437/486 (89%), Positives = 440/486 (90%)
 Frame = +1

Query: 145  MDLQIAXXXXXXXXXXXXXXXXXXXXXXXXXXHPWPLIGNLPTLFTNTEVPLHITLANMA 324
            MDLQIA                          HPWPLIGNLP LFTNTEVPLHITLANMA
Sbjct: 1    MDLQIALFSLIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMA 60

Query: 325  RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDC 504
            RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDC
Sbjct: 61   RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDC 120

Query: 505  TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIEFLRSKEGQVVKISQFVFGTLLNI 684
            TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELI+FLRSKEGQVVKISQFVFGTLLNI
Sbjct: 121  TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNI 180

Query: 685  LGNVVFSKDVFVYSDETDKGGIQNLIREMLMIGAEPNVAEFYPILEELDLQGLKKKCDDR 864
            LGNVVFSKDVFVY DETDKGGIQNLIREMLMIGAEPNVAEFYP LEELDLQGLKKKCD+R
Sbjct: 181  LGNVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDER 240

Query: 865  FIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 1044
            FIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE
Sbjct: 241  FIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 300

Query: 1045 WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMXXXXXXXXXXXX 1224
            WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAM            
Sbjct: 301  WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLLPR 360

Query: 1225 XXXETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFRPERFLESDVDFHGAHYQFIP 1404
               ETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTF PERFLESDVDFHGAHYQFIP
Sbjct: 361  RAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQFIP 420

Query: 1405 FGSGRRTCVGMPLATRTIPLIVGSLVHNYDLGLPGGNRPEDLKMNEMLSLSLAIDPSLCV 1584
            FGSGRRTCVGMPLATRTIPLIVGSLVHNYD GLPGGNRPEDLKMNEMLSL+LAIDPSLCV
Sbjct: 421  FGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAIDPSLCV 480

Query: 1585 VPKARA 1602
            VPKARA
Sbjct: 481  VPKARA 486


>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
          Length = 346

 Score =  501 bits (1290), Expect = e-139
 Identities = 249/346 (71%), Positives = 285/346 (82%)
 Frame = +1

Query: 562  MLQAQSHVREQKVAELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDK 741
            MLQ Q HVREQKVAEL+EFL+ KEGQVVKISQ VFGTLLNILGNV+FSKDVF Y    D 
Sbjct: 1    MLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYVG-ADG 59

Query: 742  GGIQNLIREMLMIGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANR 921
              +Q LI+EMLMIGA PN+AEF+PI EE DLQGLKK+C+ RF  VMKMW+GTV+ERK  R
Sbjct: 60   DKMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVKR 119

Query: 922  NEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKE 1101
            NEE KDMLDVLL NDFNDAQINA+FLETFGPGSETSSAT EW +AELIK+P  +AKVR+E
Sbjct: 120  NEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAKVRQE 179

Query: 1102 LNEVVGTSTIKESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQV 1281
            L++VVG ST+KESDLP LPYLQACIKE M               ETC VMGYTIPK++QV
Sbjct: 180  LDQVVGKSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQV 239

Query: 1282 LVNAYAIGRDPKSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIP 1461
            LVN YAIGRDPK+WK+P+TF+PERFLESDVD++G H++FIPFG+GRR CVGMPLATRTIP
Sbjct: 240  LVNIYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHFEFIPFGAGRRQCVGMPLATRTIP 299

Query: 1462 LIVGSLVHNYDLGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKAR 1599
            LIV +LV   D  LP G  P+DL MNE LSLSLA+DP+L V+PK+R
Sbjct: 300  LIVSNLVQTLDWSLPDGKLPQDLVMNESLSLSLALDPTLTVIPKSR 345


>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
            Full=(S)-N-methylcoclaurine oxidase [C-O
            phenol-coupling]; AltName: Full=CYPLXXX; AltName:
            Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
            cytochrome P-450 CYP80 [Berberis stolonifera]
          Length = 487

 Score =  489 bits (1259), Expect = e-136
 Identities = 239/452 (52%), Positives = 321/452 (71%), Gaps = 1/452 (0%)
 Frame = +1

Query: 247  WPLIGNLPTLFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDR 426
            WP++G+LP L +    P    ++N+A+ +GP++ L  G   ++ AST EAAME+L+T+D+
Sbjct: 35   WPIVGHLPDLISKNSPPFLDYMSNIAQKYGPLIHLKFGLHSSIFASTKEAAMEVLQTNDK 94

Query: 427  IFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAE 606
            + S R     FR+K HI YS++WSD   YWK  RKI+ TEIFS KMLQAQ   RE+    
Sbjct: 95   VLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGRKILHTEIFSQKMLQAQEKNRERVAGN 154

Query: 607  LIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDKGGIQNLIREMLMIGA 786
            L+ F+ +K G VV++  ++FG  LN+LG+VVFSKDVF YSD++D+ G+  LI  MLM G 
Sbjct: 155  LVNFIMTKVGDVVELRSWLFGCALNVLGHVVFSKDVFEYSDQSDEVGMDKLIHGMLMTGG 214

Query: 787  EPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANRNEESKDMLDVLLAND 966
            + +VA ++P+L   DL GLK+K D++F  ++K+WEG V  R+ANRN E KDMLDVL+AND
Sbjct: 215  DFDVASYFPVLARFDLHGLKRKMDEQFKLLIKIWEGEVLARRANRNPEPKDMLDVLIAND 274

Query: 967  FNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVG-TSTIKESD 1143
            FN+ QINA+F+ETFGPGS+T+S  IEW +A+LIK+P ++AK+R+EL+ VVG +ST+KES 
Sbjct: 275  FNEHQINAMFMETFGPGSDTNSNIIEWALAQLIKNPDKLAKLREELDRVVGRSSTVKESH 334

Query: 1144 LPQLPYLQACIKEAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAIGRDPKSW 1323
              +LPYLQAC+KE M               ETC+VMGYTIPK   V VNA+AIGRDPK W
Sbjct: 335  FSELPYLQACVKETMRLYPPISIMIPHRCMETCQVMGYTIPKGMDVHVNAHAIGRDPKDW 394

Query: 1324 KDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDLGL 1503
            KDP  F+PERFL+SD++++G  +QFIPFGSGRR C G PLA R IPL++ SLVH +   L
Sbjct: 395  KDPLKFQPERFLDSDIEYNGKQFQFIPFGSGRRICPGRPLAVRIIPLVLASLVHAFGWEL 454

Query: 1504 PGGNRPEDLKMNEMLSLSLAIDPSLCVVPKAR 1599
            P G   E L M E+ +LSL +   L V+PK R
Sbjct: 455  PDGVPNEKLDMEELFTLSLCMAKPLRVIPKVR 486


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2; AltName: Full=Cytochrome P450 80B2
            gi|9971208|dbj|BAB12433.1|
            (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  432 bits (1110), Expect = e-118
 Identities = 223/457 (48%), Positives = 305/457 (66%), Gaps = 4/457 (0%)
 Frame = +1

Query: 244  PWPLIGNLPTLFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHD 423
            P P++GNL  L        H   A +A+ +G +  L LG+Q  V+AS+  AA EILKTHD
Sbjct: 35   PSPIVGNLLQLGDKP----HAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHD 90

Query: 424  RIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVA 603
            +I S R++  SFR+K H++ S+VWS+C D WKLLRK+ RTE+F+PKM+++QS +RE K  
Sbjct: 91   KILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAR 150

Query: 604  ELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDKGGIQNL---IREML 774
            E+++FLR KEG+VVKI + VFGTL+NI GN++FSKDVF   D T  GG   L   + ++L
Sbjct: 151  EMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPT--GGSVELKEHLWKLL 208

Query: 775  MIGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANRNEESK-DMLDV 951
             +G   N A+++PI+ +LDL G ++   +   ++  +W   +KER+  +  ESK D +DV
Sbjct: 209  DMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGSESKNDFVDV 268

Query: 952  LLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTI 1131
            LL    +D +INAL +E FG G+ETS++TIEW I EL K P  ++K+R EL  VVG +T+
Sbjct: 269  LLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTV 328

Query: 1132 KESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAIGRD 1311
            KESDLP LPYLQA +KE +               ETC VM YTIPK  Q++VNA+AIGRD
Sbjct: 329  KESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRD 388

Query: 1312 PKSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNY 1491
            PK+W DP  F+PERFL SDVD+ G  ++ IPFG GRR C G+PLA++   LIV +LV N+
Sbjct: 389  PKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNF 448

Query: 1492 DLGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKARA 1602
            +  LP G    +L M+E   L+L  DP L +V KARA
Sbjct: 449  EWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARA 485


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  428 bits (1101), Expect = e-117
 Identities = 221/456 (48%), Positives = 302/456 (66%), Gaps = 4/456 (0%)
 Frame = +1

Query: 244  PWPLIGNLPTLFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHD 423
            P P++GNL  L            A +A+ +G +  L LG+Q  V+AS+  AA E+LKTHD
Sbjct: 35   PLPIVGNLLQLGDKPRAEF----AKLAQKYGQLFSLKLGSQTVVVASSPAAAAEVLKTHD 90

Query: 424  RIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVA 603
            +I S R++  SFR+K H++ S+VWS+C D WKLLRK+ RTE+F+PKM+++QS +RE K  
Sbjct: 91   KILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREGKAR 150

Query: 604  ELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDKGGIQNL---IREML 774
            E+++FLRSKEG+VVKI + VFGTL+NI GN++FSKDVF   D T  GG   L   + ++L
Sbjct: 151  EMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPT--GGSVELKEHLWKLL 208

Query: 775  MIGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANRNEESK-DMLDV 951
             +G   N A+++PI+ + DL G ++   D   ++  +W   +KER+  +  ES  D +DV
Sbjct: 209  EMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESNNDFVDV 268

Query: 952  LLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTI 1131
            LL     D +INAL +E FG G+ETS++TIEW I EL K P  ++K+R EL  VVG +T+
Sbjct: 269  LLNAGLEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTV 328

Query: 1132 KESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAIGRD 1311
            KESDLP LPYLQA +KE +               ETC VM YTIPK  Q++VNA+AIGRD
Sbjct: 329  KESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRD 388

Query: 1312 PKSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNY 1491
            PK+W DP  F+PERFL SDVD+ G  ++ IPFG GRR C G+PLA++ I LIV +LV N+
Sbjct: 389  PKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFISLIVATLVQNF 448

Query: 1492 DLGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKAR 1599
            +  LP G    +L M+E   L+L  DP L +V KAR
Sbjct: 449  EWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484