BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00001576
         (1199 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]   342   1e-91
gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]             342   1e-91
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   273   5e-71
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...   270   5e-70
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra...   269   9e-70

>gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]
          Length = 356

 Score =  342 bits (877), Expect = 1e-91
 Identities = 169/328 (51%), Positives = 235/328 (71%), Gaps = 4/328 (1%)
 Frame = -1

Query: 1085 DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIA----LSSLATSPTLVSI 918
            +MK  +QI N +CG VD+ VL+CA++L +FD I N+   KP+     LSS+ +SP+  SI
Sbjct: 11   EMKYQSQIWNQICGTVDTSVLRCAIQLGIFDAIHNS--GKPMITLTELSSIVSSPSSSSI 68

Query: 917  KPNNLYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLIRDQKRSLVDWALAIIDETVID 738
            +P NLYRL+RYL  M+L++I    N+ T SLT  SKLL+R+Q++SL+DW LAI  E ++ 
Sbjct: 69   EPCNLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLAIXCEMMVV 128

Query: 737  GWHELSGCCTSPAGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCC 558
             WHELS   ++PA  P  F++VHGK+  ELAGE    N +IN+AM SDT +  P  +Q C
Sbjct: 129  VWHELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVTKPALIQGC 188

Query: 557  DKLLNGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIAASAQLPGVEMVGGDMFK 378
             K+LNG+TS++D+GGG G TM+YIV+AFPHIK  V DLPHV+ A+ + PGVE + GD+FK
Sbjct: 189  GKILNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERPGVEFISGDIFK 248

Query: 377  FIPPADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQEDDLTRAK 198
             I  ADA+ LK++LHNW D EC+ +LK+CKE +P DKGKVII+D+V D ++    LT+AK
Sbjct: 249  SISNADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVID-DDDNSILTQAK 307

Query: 197  MNLDIDMMVTSGGRERTENEWEVLLRAS 114
            ++LD+ +M   GGRERT+ +W  L+  S
Sbjct: 308  LSLDLTVMNHGGGRERTKEDWRNLIEMS 335


>gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]
          Length = 356

 Score =  342 bits (876), Expect = 1e-91
 Identities = 169/328 (51%), Positives = 235/328 (71%), Gaps = 4/328 (1%)
 Frame = -1

Query: 1085 DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIA----LSSLATSPTLVSI 918
            +MK  +QI N +CG VD+ VL+CA++L +FD I N+   KP+     LSS+ +SP+  SI
Sbjct: 11   EMKYQSQIWNQICGTVDTSVLRCAIQLGIFDAIHNS--GKPMITLTELSSIVSSPSSSSI 68

Query: 917  KPNNLYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLIRDQKRSLVDWALAIIDETVID 738
            +P NLYRL+RYL  M+L++I    N+ T SLT  SKLL+R+Q++SL+DW LAI  E ++ 
Sbjct: 69   EPCNLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLAISCEMMVV 128

Query: 737  GWHELSGCCTSPAGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCC 558
             WHELS   ++PA  P  F++VHGK+  ELAGE    N +IN+AM SDT +  P  +Q C
Sbjct: 129  VWHELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVTKPALIQGC 188

Query: 557  DKLLNGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIAASAQLPGVEMVGGDMFK 378
             K+LNG+TS++D+GGG G TM+YIV+AFPHIK  V DLPHV+ A+ + PGVE + GD+FK
Sbjct: 189  GKILNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERPGVEFISGDIFK 248

Query: 377  FIPPADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQEDDLTRAK 198
             I  ADA+ LK++LHNW D EC+ +LK+CKE +P DKGKVII+D+V D ++    LT+AK
Sbjct: 249  SISNADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVID-DDDNSILTQAK 307

Query: 197  MNLDIDMMVTSGGRERTENEWEVLLRAS 114
            ++LD+ +M   GGRERT+ +W  L+  S
Sbjct: 308  LSLDLTVMNHGGGRERTKEDWRNLIEMS 335


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase 2 [Papaver somniferum]
          Length = 357

 Score =  273 bits (699), Expect = 5e-71
 Identities = 137/323 (42%), Positives = 206/323 (63%), Gaps = 2/323 (0%)
 Frame = -1

Query: 1082 MKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIALSSLATSPTLVSIKPNNL 903
            +K  AQ+ N + G  DS+VL+CAVE+ + D+I NN  +  I L+ LA    + ++  + L
Sbjct: 17   IKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGA--ITLAQLAAKLPITNVSSDYL 74

Query: 902  YRLLRYLVHMNLLTIHV--EGNDETFSLTELSKLLIRDQKRSLVDWALAIIDETVIDGWH 729
            YR++RYLVH+N++       G ++ +SL  +  LL+RD +RS+V   L +  +  +  WH
Sbjct: 75   YRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWH 134

Query: 728  ELSGCCTSPAGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKL 549
             +     +  G  T FE+  G  +W+    N   +Q+ N+ M  +T L+    ++ C   
Sbjct: 135  FMKEGLGN--GSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDT 192

Query: 548  LNGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIAASAQLPGVEMVGGDMFKFIP 369
              G+ S+VDIGGG G T+  I +AFPHIKCT++DLPHV+A S  LP +E V GDMFK +P
Sbjct: 193  FQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVP 252

Query: 368  PADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQEDDLTRAKMNL 189
             A AI LK +LH+W D+EC+ ILKKCKE IP++ GKVII+D+  ++ E   +LT+ ++ L
Sbjct: 253  SAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEE-ESNHELTKTRLIL 311

Query: 188  DIDMMVTSGGRERTENEWEVLLR 120
            DIDM+V +GGRERT ++WE LL+
Sbjct: 312  DIDMLVNTGGRERTADDWENLLK 334


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
            glaucum]
          Length = 350

 Score =  270 bits (690), Expect = 5e-70
 Identities = 135/321 (42%), Positives = 201/321 (62%)
 Frame = -1

Query: 1085 DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIALSSLATSPTLVSIKPNN 906
            ++ S A++ N + G  DS+VLK AV+L+L ++I N+    P+ LS L+       +  + 
Sbjct: 8    NLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNH--GSPMTLSELSLHLPSQPVNQDA 65

Query: 905  LYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLIRDQKRSLVDWALAIIDETVIDGWHE 726
            LYR+LRYLVHM L T      +  + L   +K L++   + ++   L I D+  +  WH 
Sbjct: 66   LYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPWHY 125

Query: 725  LSGCCTSPAGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKLL 546
            L     +     T FE+  G ++W+   E+   NQ+ N+ M +DT L+M   V+ C  + 
Sbjct: 126  LKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSSMF 185

Query: 545  NGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIAASAQLPGVEMVGGDMFKFIPP 366
            +GIT++VD+GGG G  +  I KAFPHIKCTV+DLPHVIA S     +  + GDMFK+IP 
Sbjct: 186  DGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKYIPN 245

Query: 365  ADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQEDDLTRAKMNLD 186
            ADAI +K +LH+W DKECI ILK+CK+ +P+D GKVII+DI+ D  + E   T+ ++ LD
Sbjct: 246  ADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDV-KSEHPYTKMRLTLD 304

Query: 185  IDMMVTSGGRERTENEWEVLL 123
            +DMM+ +GG+ERTE EW+ L+
Sbjct: 305  LDMMLNTGGKERTEEEWKKLI 325


>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase [Thalictrum
            flavum subsp. glaucum]
          Length = 348

 Score =  269 bits (688), Expect = 9e-70
 Identities = 133/324 (41%), Positives = 211/324 (65%)
 Frame = -1

Query: 1085 DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIALSSLATSPTLVSIKPNN 906
            D+K+ A +   + G  DS+VL+CAVEL +FD+I NN  ++PI L+ LA+   + ++  +N
Sbjct: 12   DIKAQAHVWKIIYGFADSLVLRCAVELGIFDIIDNN--NQPIPLADLASKLPISNVNLDN 69

Query: 905  LYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLIRDQKRSLVDWALAIIDETVIDGWHE 726
            L+R+LRYLV M LL+      D+ ++L  ++KL++R++KRS+V   L +  +  +  WH 
Sbjct: 70   LFRILRYLVKMELLSYAA---DDKYALEPIAKLVLRNEKRSMVPMVLGMTQKDFMTPWHS 126

Query: 725  LSGCCTSPAGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKLL 546
            +    T    G T FE+  G ++WE    +   +Q+ N+ M  +T L+    +     + 
Sbjct: 127  MKDGLTD--NGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSKDMF 184

Query: 545  NGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIAASAQLPGVEMVGGDMFKFIPP 366
              I S+VD+GGG G T+  I  AFPHIKCT+FDLPHVIA S   P +E +GGDMFK +P 
Sbjct: 185  QSIDSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKGMPS 244

Query: 365  ADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQEDDLTRAKMNLD 186
            A AI LK +LH+W+D++ I ILK+C++ +P+D GKVII+D+  D+ + + +L+  ++ LD
Sbjct: 245  AQAILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDE-DSDHELSSTRLILD 303

Query: 185  IDMMVTSGGRERTENEWEVLLRAS 114
            IDM+V +GG+ERT+ +WE L++ +
Sbjct: 304  IDMLVNTGGKERTKEDWEKLVKCA 327