BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00001246
         (934 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]       394   e-107
gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver...   291   2e-76
dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]    270   3e-70
gb|AFB74617.1| cytochrome P450 [Papaver somniferum]                   258   1e-66
gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]           254   2e-65

>dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 534

 Score =  394 bits (1013), Expect = e-107
 Identities = 198/304 (65%), Positives = 232/304 (76%)
 Frame = +2

Query: 23  PAPIVFSFIVIVLICIVKKRSGNVIKTRKAPEVSGAWPLVGHLNLVSGPKLVHFVLGELT 202
           PAPI+ SFIVI+L    KKRS N+I+T+KAPEV GAWP++GHLNL+S PK  + VLGEL 
Sbjct: 16  PAPIIVSFIVILLFYFFKKRSSNMIRTKKAPEVVGAWPVIGHLNLLSVPKPAYIVLGELA 75

Query: 203 DKYGPAFTIHLGMYPTFVVSNWELVKECFTTNDKIFSNRPVNKAIKYMFYNEESIGFTPY 382
           D+YGPAF+I  G++P  VVS+WELVK CFTTNDK FS+R VNKAIKYMFY++++I F P+
Sbjct: 76  DQYGPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSSRLVNKAIKYMFYDQKTISFAPH 135

Query: 383 GSYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNKDDNVWVLVDMSKW 562
           G YWRELRKM TL LLSN RL MLK  RISEMDAC + LYEL TK KD+N  VLVDMSKW
Sbjct: 136 GPYWRELRKMITLNLLSNERLKMLKHQRISEMDACLKKLYELSTKRKDENAGVLVDMSKW 195

Query: 563 FGEISFNVVARIVAGKKNFGSKGDRYKKVMEEVVRLMCVTALSDIVPYXXXXXXXXXXXX 742
           FG+ISFNVV RIVAGK  FG K +RY  VMEE  RLM V   SD++PY            
Sbjct: 196 FGDISFNVVTRIVAGKHIFGPKTERYMNVMEEARRLMDVMVFSDVIPYLGWLDRLRGVDS 255

Query: 743 AMKRAAKELDSVLESWVEEHRQRRVSVSAGTGSTANTTKEEVEKDFIDITLSIMEENQLP 922
            +KR AKELDS LESWV+EHRQ+RVS+SAG G   N T+EE E DFIDI LSI+ +N+L 
Sbjct: 256 EIKRTAKELDSALESWVDEHRQKRVSISAGIGGIVNITEEE-EIDFIDIMLSIIAKNKLL 314

Query: 923 GDDP 934
           GDDP
Sbjct: 315 GDDP 318


>gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver somniferum]
          Length = 526

 Score =  291 bits (744), Expect = 2e-76
 Identities = 148/307 (48%), Positives = 200/307 (65%), Gaps = 5/307 (1%)
 Frame = +2

Query: 29  PIVFSFIVIVLICIVKKRSGNVIKTRK-APEVSGAWPLVGHLNLVSGPKLVHFVLGELTD 205
           PI  + +VI L+    +R+     T+K APE SG  P++GHL+L +  +L H  LG + D
Sbjct: 21  PISVALVVIALVWNYGRRN----PTKKLAPEASGGRPIMGHLHLFNDGELTHRKLGAMAD 76

Query: 206 KYGPAFTIHLGMYPTFVVSNWELVKECFTTNDKIFSNRPVNKAIKYMFYNEESIGFTPYG 385
            YGP F I  G + T VVS+WE+VKECFTTNDK+FSNRP    IK MFY+ +S+G+ PYG
Sbjct: 77  TYGPVFNIRFGSHKTLVVSDWEIVKECFTTNDKLFSNRPGTLGIKLMFYDADSVGYAPYG 136

Query: 386 SYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNKDDNVWVLVDMSKWF 565
           +YWR+LRK++TLKLLSNHR+D +K LR SE+++CF +LY  W   +    +  V M  W 
Sbjct: 137 AYWRDLRKISTLKLLSNHRIDTIKHLRSSEVESCFESLYSQWGNGEKSGEFAPVRMDSWL 196

Query: 566 GEISFNVVARIVAGKKNFGSKGD----RYKKVMEEVVRLMCVTALSDIVPYXXXXXXXXX 733
           G+++FNVVARIVAGKKNF + GD    RYK  M+E +RLM   A SD++P          
Sbjct: 197 GDLTFNVVARIVAGKKNFSANGDVGAQRYKAAMDEAMRLMRFFAFSDVIPSLSWLDNLRG 256

Query: 734 XXXAMKRAAKELDSVLESWVEEHRQRRVSVSAGTGSTANTTKEEVEKDFIDITLSIMEEN 913
               MK+ A E+DS++ +WVEEHR +R   ++G  S       ++E DFID+ L IME +
Sbjct: 257 LVREMKKCASEIDSIMATWVEEHRVKR---NSGGNS-------QLEHDFIDVCLDIMEHS 306

Query: 914 QLPGDDP 934
            LPGDDP
Sbjct: 307 SLPGDDP 313


>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
          Length = 524

 Score =  270 bits (691), Expect = 3e-70
 Identities = 139/317 (43%), Positives = 199/317 (62%), Gaps = 7/317 (2%)
 Frame = +2

Query: 2   LPTSIIAPAPIVFSFIVIVLICIVKKRSGNVIKTRKAPEVSGAWPLVGHLNLVSGPKLVH 181
           L  + + P  +      + L  +   R+    K R AP  +GAWP++GHL+L    +L H
Sbjct: 4   LMLAYLFPISVASIIAFVFLYNLFSSRTLKNKKIRTAPMATGAWPVLGHLHLFGSGELPH 63

Query: 182 FVLGELTDKYGPAFTIHLGMYPTFVVSNWELVKECFTTNDKIFSNRPVNKAIKYMFYNEE 361
            +L  + DKYG AF +  G + T VVS+  +VKECFTTND +FSNRP  KA + M Y+ E
Sbjct: 64  KMLAAMADKYGSAFRMKFGKHTTLVVSDTRIVKECFTTNDTLFSNRPSTKAFQLMTYDNE 123

Query: 362 SIGFTPYGSYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNKDDNVWV 541
           S+ FTPYGSYWRE+RK++TLKLLSNHRL  +K +R SE++ CF+ LY+   KN   +  +
Sbjct: 124 SVAFTPYGSYWREIRKISTLKLLSNHRLQAIKDVRASEVNVCFKTLYD-QCKNPSGSAPI 182

Query: 542 LVDMSKWFGEISFNVVARIVAGKKNFGSK-------GDRYKKVMEEVVRLMCVTALSDIV 700
           L+DM KWF E+S NVV R++ G++NFGSK          YKKVM+E++RL  ++  SD  
Sbjct: 183 LIDMKKWFEEVSNNVVMRVIVGRQNFGSKIVQGEEEAIHYKKVMDELLRLASLSMFSDFA 242

Query: 701 PYXXXXXXXXXXXXAMKRAAKELDSVLESWVEEHRQRRVSVSAGTGSTANTTKEEVEKDF 880
           P             AMKR AK++D++LE+W+EEHR+++ SV+            E ++DF
Sbjct: 243 PLLGFVDIFQGNLSAMKRNAKKVDAILENWLEEHRKKKNSVA------------ESQQDF 290

Query: 881 IDITLSIMEENQLPGDD 931
           +D+ LSI+EE++L G D
Sbjct: 291 MDVMLSIVEESKLSGHD 307


>gb|AFB74617.1| cytochrome P450 [Papaver somniferum]
          Length = 556

 Score =  258 bits (659), Expect = 1e-66
 Identities = 140/297 (47%), Positives = 191/297 (64%), Gaps = 11/297 (3%)
 Frame = +2

Query: 77  KRSGNVIKTRKAPEVSGAWPLVGHLNLVSGPK-LVHFVLGELTDKYGPAFTIHLGMYPTF 253
           +R+   I T   PE SGAWP++GHL L      L H  LG + DKYGP F++  G + T 
Sbjct: 46  RRNRTAITTTPLPEASGAWPVIGHLLLFMNENDLNHVTLGHMADKYGPIFSLRFGRHRTL 105

Query: 254 VVSNWELVKECFT-TNDKIFSNRPVNKAIKYMFYNEESIGFTPYGSYWRELRKMTTLKLL 430
           VVS+WE+VKECFT TNDK+FSNRP + A+K MFY+ ES GF PYG YWRELRK++T KLL
Sbjct: 106 VVSSWEMVKECFTGTNDKLFSNRPSSLAVKLMFYDTESYGFAPYGKYWRELRKISTHKLL 165

Query: 431 SNHRLDMLKPLRISEMDACFRNLYELWTKNKD--DNVWV--LVDMSKWFGEISFNVVARI 598
           SN +L+  K LRISE+D  F+ L+EL + NK   D  +V  LV M  WF  ++FNV+ RI
Sbjct: 166 SNQQLEKFKHLRISEVDNSFKKLHELCSNNKQGGDTTYVASLVRMDDWFAYLTFNVIGRI 225

Query: 599 VAGKKNFGSKG-----DRYKKVMEEVVRLMCVTALSDIVPYXXXXXXXXXXXXAMKRAAK 763
           V+G ++    G     ++YK  ++EV  LM   A+SD+VP              MK   K
Sbjct: 226 VSGFQSNAVAGATNSQEKYKLAIDEVSNLMATFAVSDVVPRLGWIDRLTGLTGKMKNCGK 285

Query: 764 ELDSVLESWVEEHRQRRVSVSAGTGSTANTTKEEVEKDFIDITLSIMEENQLPGDDP 934
           +LD+V+   VE+HRQ+++ +S    +T   T+ E E+DFID+ LSIME++Q+PG+ P
Sbjct: 286 KLDAVVGDAVEDHRQKKLKIS--RNNTGALTEHE-EEDFIDVCLSIMEQSQIPGNHP 339


>gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]
          Length = 541

 Score =  254 bits (649), Expect = 2e-65
 Identities = 132/290 (45%), Positives = 181/290 (62%), Gaps = 8/290 (2%)
 Frame = +2

Query: 86  GNVIKTRKAPEVSGAWPLVGHLNLVSGPKLVHFVLGELTDKYGPAFTIHLGMYPTFVVSN 265
           G  I TR  P V+GAWP+ GHL+L    +  H +L +L +KYGP+FT+  G + T VVS+
Sbjct: 47  GKKIITRP-PAVTGAWPVFGHLHLFGSGEHPHEMLSKLAEKYGPSFTMKFGKHTTLVVSD 105

Query: 266 WELVKECFTTNDKIFSNRPVNKAIKYMFYNEESIGFTPYGSYWRELRKMTTLKLLSNHRL 445
             +VKECFTTND +FSNRP   A   M Y  +SI FTPY  YWRELRK++TLKLLSN+RL
Sbjct: 106 TRVVKECFTTNDTLFSNRPSTIAFDLMTYATDSIAFTPYSPYWRELRKISTLKLLSNNRL 165

Query: 446 DMLKPLRISEMDACFRNLYELWTKNKDDNVWVLVDMSKWFGEISFNVVARIVAGKKNFGS 625
           + +K LR SE+  CF+ LY+L  K  D+   V +D+ +WF E+S NV+ R++ GK+NFGS
Sbjct: 166 ESIKQLRTSEVSVCFKELYDLTNKKNDNGAPVPIDLKRWFDEVSNNVIMRVIFGKQNFGS 225

Query: 626 K--------GDRYKKVMEEVVRLMCVTALSDIVPYXXXXXXXXXXXXAMKRAAKELDSVL 781
           K           YKK+M+E+ RL  +T LSD+VP             AMKR  KEL+S+L
Sbjct: 226 KIVLGEDQEAVHYKKIMDELSRLSSLTMLSDMVPLLGWLDYFKGDLRAMKRNGKELNSIL 285

Query: 782 ESWVEEHRQRRVSVSAGTGSTANTTKEEVEKDFIDITLSIMEENQLPGDD 931
           + W+EEH+ ++ S              +  +DF+D+ LSI ++ QL G D
Sbjct: 286 QKWLEEHKSKKSS--------------DARQDFMDVMLSISKDTQLYGHD 321