BLASTX nr result
ID: Coptis23_contig00001147
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00001147
(1992 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFP49810.1| 4-hydroxycinnamoyl-CoA ligase 3 [Coffea arabica] 864 0.0
gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus] 862 0.0
emb|CAN69130.1| hypothetical protein VITISV_039323 [Vitis vinifera] 859 0.0
gb|AEM63673.1| 4-hydroxycinnamoyl CoA ligase [Platycodon grandif... 858 0.0
ref|XP_002272782.1| PREDICTED: 4-coumarate--CoA ligase 1 isoform... 858 0.0
>gb|AFP49810.1| 4-hydroxycinnamoyl-CoA ligase 3 [Coffea arabica]
Length = 543
Score = 864 bits (2232), Expect = 0.0
Identities = 422/535 (78%), Positives = 479/535 (89%)
Frame = +2
Query: 140 EPQESIFRSKLPDIYIPNHLPLHTYCFENIAQFSTRPCIINGPTGDTYTYEDVELIARKV 319
+ QE +FRSKLPDIYIPNHLPLH+YCFENI +F RPC+ING TGD YTY DVELIARKV
Sbjct: 6 DQQEFVFRSKLPDIYIPNHLPLHSYCFENIPEFCNRPCLINGTTGDIYTYADVELIARKV 65
Query: 320 ASGLNKIGIQQGQVIMLLLPNSPEFVFAFLGASYRGVIITTANPFYTASEIAKQAKASDT 499
A+GL+KIGIQQG+VIMLLL NSPEF FA LGASYRG + TTANPFY +EI KQA+AS
Sbjct: 66 AAGLDKIGIQQGEVIMLLLQNSPEFAFALLGASYRGAMSTTANPFYKPAEIEKQARASKA 125
Query: 500 RLIITLASYVDKLKELAQDEEVKIMCIDSPPEGCLHFSELSQSDETELPEVKIRPDDIVA 679
+LIIT + YV+K+ + A++ VK+MC D+PPEGCLHFSELS +DE +P VKI P+D VA
Sbjct: 126 KLIITQSCYVEKVMDFAKENNVKVMCTDAPPEGCLHFSELSSADEKVIPAVKINPNDAVA 185
Query: 680 LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHQDDVLLCVLPLFHIYSLNSVL 859
LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFH++DV+LCVLPLFHIYSLNSVL
Sbjct: 186 LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFHIYSLNSVL 245
Query: 860 LCGLRAGAAILIMPKFEIKQLMELVEKYKVTIAPFVPPIVLAIAKSPDVEKYDLSSIRIL 1039
LCGLR GAAILIM KFEI LMELV+KYKVTIAPFVPPIVL IAKSP V+KYDLSSIR++
Sbjct: 246 LCGLRVGAAILIMQKFEINALMELVQKYKVTIAPFVPPIVLEIAKSPVVDKYDLSSIRMV 305
Query: 1040 MSGAAPMGKELEDTMRRKLPIAKLGQGYGMTEAGPVLSMCLAFAKEPLDIKSGSCGTVVR 1219
MSGAAPMGKELEDT+R KLP A LGQGYGMTEAGP+LSMCLAFAKEP D+KSG+CGTVVR
Sbjct: 306 MSGAAPMGKELEDTVRAKLPKAVLGQGYGMTEAGPLLSMCLAFAKEPFDVKSGACGTVVR 365
Query: 1220 NAEMKIIDIENGTSLPRNESGEICIRGDQIMKGYLNDPKATANTIDRDGWLHTGDVGFID 1399
NAEMKI+D E SLPRN++GEICIRGDQIMKGYLNDP+AT NTID++GWLHTGD+G+ID
Sbjct: 366 NAEMKIVDPETNLSLPRNQAGEICIRGDQIMKGYLNDPEATENTIDKEGWLHTGDIGYID 425
Query: 1400 DDEELFIVDRLKELIKYKGFQVAPAELEALLINHPNISDAAVVPMKDDAAGEVPVAFVVR 1579
DD+E+FIVDRLKELIKYKGFQVAPAELEA+L++HP ISDAAVV MKD+AAGEVPVAFVVR
Sbjct: 426 DDDEIFIVDRLKELIKYKGFQVAPAELEAMLLSHPGISDAAVVSMKDEAAGEVPVAFVVR 485
Query: 1580 SNGSQISEEEIKQYISKQVVFYKRISKVFFSETIPKGPSGKILRKELRQILAAGV 1744
++GS+ISE+EIKQ+IS QV+FYKRI +VFF + IPK PSGKILRK+LR LAA V
Sbjct: 486 ASGSKISEDEIKQFISNQVIFYKRIHRVFFMDKIPKAPSGKILRKDLRAKLAAEV 540
>gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus]
Length = 544
Score = 862 bits (2226), Expect = 0.0
Identities = 423/538 (78%), Positives = 485/538 (90%), Gaps = 1/538 (0%)
Frame = +2
Query: 140 EPQESIFRSKLPDIYIPNHLPLHTYCFENIAQFSTRPCIINGPTGDTYTYEDVELIARKV 319
+ E IFRSKLPDIYIPNHLPLHTYCFENI+QF RPC+ING TG+T+TY +VEL +R+V
Sbjct: 7 DDHEFIFRSKLPDIYIPNHLPLHTYCFENISQFHDRPCLINGNTGETFTYAEVELTSRRV 66
Query: 320 ASGLNKIGIQQGQVIMLLLPNSPEFVFAFLGASYRGVIITTANPFYTASEIAKQAKASDT 499
A+GL+K+GIQQ V+MLLL N PEF FAFLGASY G + TTANPFYT +E+AKQAKAS+
Sbjct: 67 AAGLDKLGIQQNDVVMLLLQNCPEFAFAFLGASYIGAMSTTANPFYTPAEVAKQAKASNA 126
Query: 500 RLIITLASYVDKLKELAQDEEVKIMCID-SPPEGCLHFSELSQSDETELPEVKIRPDDIV 676
+LIIT ++YVDK+K+ A+ +VK+MC+D + E LHFSEL +DE+E P VKI PDD+V
Sbjct: 127 KLIITQSAYVDKVKDFAKLNDVKVMCVDETSSEDVLHFSELMSADESETPAVKINPDDVV 186
Query: 677 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHQDDVLLCVLPLFHIYSLNSV 856
ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFH++DV+LCVLPLFHIYSLNSV
Sbjct: 187 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFHIYSLNSV 246
Query: 857 LLCGLRAGAAILIMPKFEIKQLMELVEKYKVTIAPFVPPIVLAIAKSPDVEKYDLSSIRI 1036
LCGLR GAAILIM KFEI +L+ELVEK KVTIAPFVPPIVL+IAK PD+ +YDLSSIR+
Sbjct: 247 FLCGLRVGAAILIMQKFEINKLLELVEKEKVTIAPFVPPIVLSIAKCPDLHRYDLSSIRM 306
Query: 1037 LMSGAAPMGKELEDTMRRKLPIAKLGQGYGMTEAGPVLSMCLAFAKEPLDIKSGSCGTVV 1216
+MSGAAPMGKELEDT+R KLP AKLGQGYGMTEAGPVLSMCLAFAKEP +IKSG+CGTVV
Sbjct: 307 VMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPYEIKSGACGTVV 366
Query: 1217 RNAEMKIIDIENGTSLPRNESGEICIRGDQIMKGYLNDPKATANTIDRDGWLHTGDVGFI 1396
RNAEMKIID + SLPRN+SGEICIRG QIMKGYLNDP+AT NTID++GWLHTGD+G+I
Sbjct: 367 RNAEMKIIDPDTNESLPRNQSGEICIRGSQIMKGYLNDPEATENTIDKEGWLHTGDIGYI 426
Query: 1397 DDDEELFIVDRLKELIKYKGFQVAPAELEALLINHPNISDAAVVPMKDDAAGEVPVAFVV 1576
DDD+ELFIVDRLKELIKYKGFQVAPAELEA+LI+HPN+SDAAVV MKD+AAGEVPVAFVV
Sbjct: 427 DDDDELFIVDRLKELIKYKGFQVAPAELEAMLISHPNLSDAAVVSMKDEAAGEVPVAFVV 486
Query: 1577 RSNGSQISEEEIKQYISKQVVFYKRISKVFFSETIPKGPSGKILRKELRQILAAGVPS 1750
RSNGS+ISE++IKQYISKQVVFYKRISKVFF++ IPK PSGKILRK+LR LAAG+P+
Sbjct: 487 RSNGSKISEDDIKQYISKQVVFYKRISKVFFTDKIPKAPSGKILRKDLRARLAAGLPN 544
>emb|CAN69130.1| hypothetical protein VITISV_039323 [Vitis vinifera]
Length = 548
Score = 859 bits (2219), Expect = 0.0
Identities = 420/537 (78%), Positives = 478/537 (89%)
Frame = +2
Query: 140 EPQESIFRSKLPDIYIPNHLPLHTYCFENIAQFSTRPCIINGPTGDTYTYEDVELIARKV 319
E +E IFRSKLPDIYIP+HLPLH+YCFENI++FS+RPC+I+G GD YTY DVEL AR+V
Sbjct: 12 EQREIIFRSKLPDIYIPDHLPLHSYCFENISKFSSRPCVIDGANGDIYTYADVELTARRV 71
Query: 320 ASGLNKIGIQQGQVIMLLLPNSPEFVFAFLGASYRGVIITTANPFYTASEIAKQAKASDT 499
+GL+K+GI+QG+VIMLLL N EFVFAFLGASY G TTANPFY +EI KQAKAS
Sbjct: 72 GAGLHKMGIKQGEVIMLLLQNCTEFVFAFLGASYIGATSTTANPFYMPAEIEKQAKASKC 131
Query: 500 RLIITLASYVDKLKELAQDEEVKIMCIDSPPEGCLHFSELSQSDETELPEVKIRPDDIVA 679
RLIIT A Y K A++ +VK+MCID+PPEGCLHFSEL+ ++E +LP VKI PDD+VA
Sbjct: 132 RLIITQACYSGKAMSFAEENDVKVMCIDTPPEGCLHFSELTNABEADLPTVKINPDDVVA 191
Query: 680 LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHQDDVLLCVLPLFHIYSLNSVL 859
LPYSSGTTGLPKGVMLTH+G VTSVAQQVDGENPNLYFH +DV+LCVLPLFH+YSLNSVL
Sbjct: 192 LPYSSGTTGLPKGVMLTHRGQVTSVAQQVDGENPNLYFHSEDVILCVLPLFHVYSLNSVL 251
Query: 860 LCGLRAGAAILIMPKFEIKQLMELVEKYKVTIAPFVPPIVLAIAKSPDVEKYDLSSIRIL 1039
LCGLR GAAILIM KFEI +L+EL+ KYKVTIAPFVPPIVLAIAKSP V++YDLSSIR +
Sbjct: 252 LCGLRVGAAILIMQKFEIIKLLELINKYKVTIAPFVPPIVLAIAKSPVVDEYDLSSIRTV 311
Query: 1040 MSGAAPMGKELEDTMRRKLPIAKLGQGYGMTEAGPVLSMCLAFAKEPLDIKSGSCGTVVR 1219
MSGAAPMGKELEDT+R KLP AKLGQGYGMTEAGPVL+MCLAFAKEP +IKSGSCGTVVR
Sbjct: 312 MSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGSCGTVVR 371
Query: 1220 NAEMKIIDIENGTSLPRNESGEICIRGDQIMKGYLNDPKATANTIDRDGWLHTGDVGFID 1399
NA+MKI+D + G SLPRN+ GEICIRG QIMKGYLNDP+AT TID++GWLHTGD+G+ID
Sbjct: 372 NAQMKILDPDTGASLPRNQPGEICIRGHQIMKGYLNDPEATERTIDKEGWLHTGDIGYID 431
Query: 1400 DDEELFIVDRLKELIKYKGFQVAPAELEALLINHPNISDAAVVPMKDDAAGEVPVAFVVR 1579
DD+ELFIVDRLKELIKYKGFQVAPAELEA+LI HPN+SDAAVV MKD+AAGEVPVAF+VR
Sbjct: 432 DDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNVSDAAVVSMKDEAAGEVPVAFIVR 491
Query: 1580 SNGSQISEEEIKQYISKQVVFYKRISKVFFSETIPKGPSGKILRKELRQILAAGVPS 1750
SNGS+I+E+EIKQYISKQVVFYKRI +VFF ETIPK PSGKILRK+LR LA GVP+
Sbjct: 492 SNGSKITEDEIKQYISKQVVFYKRIYRVFFVETIPKAPSGKILRKDLRARLAVGVPN 548
>gb|AEM63673.1| 4-hydroxycinnamoyl CoA ligase [Platycodon grandiflorus]
Length = 556
Score = 858 bits (2218), Expect = 0.0
Identities = 419/535 (78%), Positives = 477/535 (89%)
Frame = +2
Query: 146 QESIFRSKLPDIYIPNHLPLHTYCFENIAQFSTRPCIINGPTGDTYTYEDVELIARKVAS 325
QE IFRSKLPDIYIPNHLPLH+YCFENI++FS RPC+I G TG YTY DVE+ +RKVAS
Sbjct: 22 QEIIFRSKLPDIYIPNHLPLHSYCFENISKFSERPCLIEGATGKVYTYADVEVTSRKVAS 81
Query: 326 GLNKIGIQQGQVIMLLLPNSPEFVFAFLGASYRGVIITTANPFYTASEIAKQAKASDTRL 505
LNK+GI QG IMLLLPNS EFVFAFLGASY G I T ANPF+T +EI KQ KAS+ ++
Sbjct: 82 SLNKLGIGQGDTIMLLLPNSAEFVFAFLGASYLGAISTMANPFFTHAEIIKQVKASNAKI 141
Query: 506 IITLASYVDKLKELAQDEEVKIMCIDSPPEGCLHFSELSQSDETELPEVKIRPDDIVALP 685
IITLA +VDK+KE A + +VK++CID PEGCLH+SEL+ DE +LPEVKI PDD+VALP
Sbjct: 142 IITLACHVDKVKEFASENDVKVICIDKAPEGCLHYSELTLGDENDLPEVKISPDDVVALP 201
Query: 686 YSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHQDDVLLCVLPLFHIYSLNSVLLC 865
YSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NLY H +DVL+CVLPLFHIYSLNS+LLC
Sbjct: 202 YSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLICVLPLFHIYSLNSILLC 261
Query: 866 GLRAGAAILIMPKFEIKQLMELVEKYKVTIAPFVPPIVLAIAKSPDVEKYDLSSIRILMS 1045
GLR GAAILIM KF+I +EL+EKYKVTI PFVPPIVLA+AKS V+KYDL+SIR +MS
Sbjct: 262 GLRVGAAILIMQKFDIVPFLELIEKYKVTIGPFVPPIVLAMAKSSHVDKYDLTSIRTVMS 321
Query: 1046 GAAPMGKELEDTMRRKLPIAKLGQGYGMTEAGPVLSMCLAFAKEPLDIKSGSCGTVVRNA 1225
GAAP+GKELEDT+R KLP AKLGQGYGMTEAGPVL+MCLAFAKEP +IKSG+CGTVVRNA
Sbjct: 322 GAAPLGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNA 381
Query: 1226 EMKIIDIENGTSLPRNESGEICIRGDQIMKGYLNDPKATANTIDRDGWLHTGDVGFIDDD 1405
EMKI+D + G SLPRN+ GEICIRGDQIMKGYLNDP+AT TID++GWLHTGD+G+IDDD
Sbjct: 382 EMKIVDPDTGDSLPRNQRGEICIRGDQIMKGYLNDPEATERTIDKEGWLHTGDIGYIDDD 441
Query: 1406 EELFIVDRLKELIKYKGFQVAPAELEALLINHPNISDAAVVPMKDDAAGEVPVAFVVRSN 1585
+ELFIVDRLKELIKYKGFQVAPAELEALL+NHPNISDAAVV M D+AAGEVPVAFVVR+N
Sbjct: 442 DELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVSMIDEAAGEVPVAFVVRAN 501
Query: 1586 GSQISEEEIKQYISKQVVFYKRISKVFFSETIPKGPSGKILRKELRQILAAGVPS 1750
GS I+E+EIKQ+ISKQVVFYKRI++VFF++TIPK PSGKILRKELR LAAG+P+
Sbjct: 502 GSIITEDEIKQFISKQVVFYKRINRVFFTDTIPKAPSGKILRKELRARLAAGIPN 556
>ref|XP_002272782.1| PREDICTED: 4-coumarate--CoA ligase 1 isoform 1 [Vitis vinifera]
Length = 548
Score = 858 bits (2216), Expect = 0.0
Identities = 419/534 (78%), Positives = 477/534 (89%)
Frame = +2
Query: 149 ESIFRSKLPDIYIPNHLPLHTYCFENIAQFSTRPCIINGPTGDTYTYEDVELIARKVASG 328
E IFRSKLPDIYIP+HLPLH+YCFENI++FS+RPC+I+G GD YTY DVEL AR+V +G
Sbjct: 15 EIIFRSKLPDIYIPDHLPLHSYCFENISKFSSRPCVIDGANGDIYTYADVELTARRVGAG 74
Query: 329 LNKIGIQQGQVIMLLLPNSPEFVFAFLGASYRGVIITTANPFYTASEIAKQAKASDTRLI 508
L+K+GI+QG+VIMLLL N EFVFAFLGASY G TTANPFY +EI KQAKAS RLI
Sbjct: 75 LHKMGIKQGEVIMLLLQNCTEFVFAFLGASYIGATSTTANPFYMPAEIEKQAKASKCRLI 134
Query: 509 ITLASYVDKLKELAQDEEVKIMCIDSPPEGCLHFSELSQSDETELPEVKIRPDDIVALPY 688
IT A Y +K A++ +VK+MCID+PPEGCLHFSEL+ +DE +LP VKI PDD+VALPY
Sbjct: 135 ITQACYSEKAMSFAEENDVKVMCIDTPPEGCLHFSELTNADEADLPTVKINPDDVVALPY 194
Query: 689 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHQDDVLLCVLPLFHIYSLNSVLLCG 868
SSGTTGLPKGVMLTH+G VTSVAQQVDG+NPNLYFH +DV+LCVLPLFH+YSLNSVLLCG
Sbjct: 195 SSGTTGLPKGVMLTHRGQVTSVAQQVDGDNPNLYFHSEDVILCVLPLFHVYSLNSVLLCG 254
Query: 869 LRAGAAILIMPKFEIKQLMELVEKYKVTIAPFVPPIVLAIAKSPDVEKYDLSSIRILMSG 1048
LR GAAILIM KFEI +L+EL+ KYKVTIAPFVPPIVLAIAKSP V++YDLSSIR +MSG
Sbjct: 255 LRVGAAILIMQKFEIIKLLELINKYKVTIAPFVPPIVLAIAKSPVVDEYDLSSIRTVMSG 314
Query: 1049 AAPMGKELEDTMRRKLPIAKLGQGYGMTEAGPVLSMCLAFAKEPLDIKSGSCGTVVRNAE 1228
AAPMGKELEDT+R KLP AKLGQGYGMTEAGPVL+MCLAFAKEP +IKSGSCGTVVRNA+
Sbjct: 315 AAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGSCGTVVRNAQ 374
Query: 1229 MKIIDIENGTSLPRNESGEICIRGDQIMKGYLNDPKATANTIDRDGWLHTGDVGFIDDDE 1408
MKI+D + G SLPRN+ GEICIRG QIMKGYLNDP+AT TID++GWLHTGD+G+IDDD+
Sbjct: 375 MKILDPDTGASLPRNQPGEICIRGHQIMKGYLNDPEATERTIDKEGWLHTGDIGYIDDDD 434
Query: 1409 ELFIVDRLKELIKYKGFQVAPAELEALLINHPNISDAAVVPMKDDAAGEVPVAFVVRSNG 1588
ELFIVDRLKELIKYKGFQVAPAELEA+LI HPN+SDAAVV MKD+AAGEVPVAF+VRSNG
Sbjct: 435 ELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNVSDAAVVSMKDEAAGEVPVAFIVRSNG 494
Query: 1589 SQISEEEIKQYISKQVVFYKRISKVFFSETIPKGPSGKILRKELRQILAAGVPS 1750
S+I+E+EIKQYISKQVVFYKRI +VFF ETIPK PSGKILRK+LR LA GVP+
Sbjct: 495 SKITEDEIKQYISKQVVFYKRIYRVFFVETIPKAPSGKILRKDLRARLAVGVPN 548