BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00001005
         (1365 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   647   0.0  
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      465   e-128
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   451   e-124
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   438   e-120
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       436   e-120

>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
            gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
            [Thalictrum flavum]
          Length = 356

 Score =  647 bits (1668), Expect = 0.0
 Identities = 309/349 (88%), Positives = 334/349 (95%)
 Frame = +3

Query: 3    KKEAIADFIKRMELGQVPDEEIRKKMRTVVEKRLKWGYKPTHEQQLAQLLSFIQSLRSME 182
            KKEA+A+ IKR+E G+V DEEIR  M+  V+KRLKWGYKPTHEQQLAQL++F QSL+ ME
Sbjct: 7    KKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGME 66

Query: 183  MAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEINMLDLYCERAQIK 362
            MAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESE+ M+DLYCERAQIK
Sbjct: 67   MAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQIK 126

Query: 363  DGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDECKKLDLSNVEIILADV 542
            DGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMD+CKKLDLSNVEIIL DV
Sbjct: 127  DGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDV 186

Query: 543  TKFETENTYDRIFAVALIEHMKNYELFLKEVSKWMAQDGLLFVEHHCHKVFAYKYEPLDD 722
            TKFETE TYDRIFAVALIEHMKNYELFLK+VS W+AQ GLLFVEHHCHKVFAY+YEPLD+
Sbjct: 187  TKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQYEPLDE 246

Query: 723  DDWYTEYIFPTGTLVMSSSSILLYFQEDVSILNHWTLSGKHPSLGFKEWLKRLDDNMDAV 902
            DDWYTEYIFP+GTLVMSSSSILLYFQEDVS++NHWTLSGKHPSLGFK+WLKRLDDN+D V
Sbjct: 247  DDWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEV 306

Query: 903  KEIFESFYGSKEKAMKWITYWRVFCIAHSEMYASNNGEEWMLSQVLFKK 1049
            KEIFESFYGSKEKAMK+ITYWRVFCIAHS+MY++NNGEEWMLSQVLFKK
Sbjct: 307  KEIFESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQVLFKK 355


>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  465 bits (1197), Expect = e-128
 Identities = 212/349 (60%), Positives = 282/349 (80%)
 Frame = +3

Query: 3    KKEAIADFIKRMELGQVPDEEIRKKMRTVVEKRLKWGYKPTHEQQLAQLLSFIQSLRSME 182
            KK AI + +K++ELG VP ++I++ +R  + +RL+WGYKPT+E+Q+A++ +   SLR M+
Sbjct: 9    KKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNLTHSLRQMK 68

Query: 183  MAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEINMLDLYCERAQIK 362
            +A EV+TLD++LYEIP+ FL IM G  LK S  YFK++STTLDE+EI MLDLYCERAQI+
Sbjct: 69   IATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQ 128

Query: 363  DGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDECKKLDLSNVEIILADV 542
            DGQS+LDLGCG G+LTLHVAQKY+ C+VT +TNSVSQKE+I +E ++ +L NVE+ LAD+
Sbjct: 129  DGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADI 188

Query: 543  TKFETENTYDRIFAVALIEHMKNYELFLKEVSKWMAQDGLLFVEHHCHKVFAYKYEPLDD 722
            T  E   TYDRI  + L EHMKNYEL L+++S+W+++DGLLF+EH CHK FAY YEPLDD
Sbjct: 189  TTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDD 248

Query: 723  DDWYTEYIFPTGTLVMSSSSILLYFQEDVSILNHWTLSGKHPSLGFKEWLKRLDDNMDAV 902
            DDW+TEY+FP GT+++ S+S  LYFQ+DVS++NHWTLSGKH S   +EWLKRLD N+D +
Sbjct: 249  DDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVI 308

Query: 903  KEIFESFYGSKEKAMKWITYWRVFCIAHSEMYASNNGEEWMLSQVLFKK 1049
            K +FE+  G++E+A+K I YWR FC++  EM+  NNGEEWM S VLFKK
Sbjct: 309  KPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK 357


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
            gi|51980204|gb|AAU20766.1| (S)-coclaurine
            N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  451 bits (1161), Expect = e-124
 Identities = 210/350 (60%), Positives = 278/350 (79%), Gaps = 1/350 (0%)
 Frame = +3

Query: 3    KKEAIADFIKRMELGQVPDEEIRKKMRTVVEKRLKWGYKPTHEQQLAQLLSFIQSLRSME 182
            KK  I + +K++ELG VPD+EI+K +R  + +RL+WG K T+E+Q+AQL++   SLR M+
Sbjct: 11   KKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLVNLTHSLRQMK 70

Query: 183  MAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEINMLDLYCERAQIK 362
            +A EV+TLD ++YE+P+ FL IM G  LK S  YFK++STTLDE+EI ML+LYCERAQIK
Sbjct: 71   IATEVETLDDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAMLELYCERAQIK 130

Query: 363  DGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDECKKLDLSNVEIILADV 542
            DG S+LDLGCG G+LTL+VAQKY+  +VT +TNSVSQKEFI +E +K +LSNVE++LAD+
Sbjct: 131  DGHSVLDLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNLSNVEVLLADI 190

Query: 543  TKFETENTYDRIFAVALIEHMKNYELFLKEVSKWMAQDGLLFVEHHCHKVFAYKYEPLDD 722
            T  +  +TYDRI  V L EHMKNYEL L+++ +WMA+DGLLFVEH CHK FAY YEP+D+
Sbjct: 191  TTHKMPDTYDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPIDE 250

Query: 723  DDWYTEYIFPTGTLVMSSSSILLYFQEDVSILNHWTLSGKHPSLGFKEWLKRLDDNMDAV 902
            DDW+TEY+FP GT+++ S+S  LYFQ+DVS++NHWTLSGKH S   +EWLKRLD N++ +
Sbjct: 251  DDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANVELI 310

Query: 903  KEIFESFYGS-KEKAMKWITYWRVFCIAHSEMYASNNGEEWMLSQVLFKK 1049
            K +F +  G  +++AMK I YWR FC++  EM+  NNGEEWM S VLFKK
Sbjct: 311  KPMFVTITGQCRQEAMKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK 360


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
            somniferum]
          Length = 351

 Score =  438 bits (1127), Expect = e-120
 Identities = 200/347 (57%), Positives = 270/347 (77%)
 Frame = +3

Query: 9    EAIADFIKRMELGQVPDEEIRKKMRTVVEKRLKWGYKPTHEQQLAQLLSFIQSLRSMEMA 188
            +A  + ++ MELG +PD+EIR+ +R  +EKRL+WGYK THE+QL+QLL  + SL+ M+MA
Sbjct: 4    KAKEELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMA 63

Query: 189  EEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEINMLDLYCERAQIKDG 368
             E++ LD +LYE P+ FL I  G  +K S GY+ DESTTLDE+EI MLDLY ERAQIKDG
Sbjct: 64   TEMENLDLKLYEAPMEFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDG 123

Query: 369  QSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDECKKLDLSNVEIILADVTK 548
            QS+LDLGCG G++ L  A K++ C+ TG+T+SV QK++I  +CK+L L+NV+++LAD+T 
Sbjct: 124  QSVLDLGCGLGAVALFGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITT 183

Query: 549  FETENTYDRIFAVALIEHMKNYELFLKEVSKWMAQDGLLFVEHHCHKVFAYKYEPLDDDD 728
            +ETE  +DRIFAV LIEHMKNY+L LK++S+WM  DGLLFVEH CHK  AY YEP+D +D
Sbjct: 184  YETEERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAED 243

Query: 729  WYTEYIFPTGTLVMSSSSILLYFQEDVSILNHWTLSGKHPSLGFKEWLKRLDDNMDAVKE 908
            WYT YIFP GTL +SS+S+LLYFQ+DVS++N WTLSGKH S   +EWLK +D N+   KE
Sbjct: 244  WYTNYIFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKE 303

Query: 909  IFESFYGSKEKAMKWITYWRVFCIAHSEMYASNNGEEWMLSQVLFKK 1049
            I  S   ++++A+K + +WR+FC+  +E++   NGEEWML+ +LFKK
Sbjct: 304  IMRSITKTEKEAIKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKK 350


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  436 bits (1120), Expect = e-120
 Identities = 201/343 (58%), Positives = 263/343 (76%)
 Frame = +3

Query: 21   DFIKRMELGQVPDEEIRKKMRTVVEKRLKWGYKPTHEQQLAQLLSFIQSLRSMEMAEEVD 200
            +  K++ELG +P++E++K MR  + +RL+WGYK T+E+Q+AQ+L    SLR M +A EVD
Sbjct: 8    ELFKKLELGLIPNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVD 67

Query: 201  TLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEINMLDLYCERAQIKDGQSIL 380
            TLD+ +YE+PL FL IM G  LK S  YFKD+STTLDE+EI M+DL+CERAQIKDG S+L
Sbjct: 68   TLDSNMYEVPLDFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKDGHSVL 127

Query: 381  DLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDECKKLDLSNVEIILADVTKFETE 560
            DLGCGHGSLT +VAQKY+   VT +TNSVSQKE+I +E ++ +LSNVE++LAD+   E  
Sbjct: 128  DLGCGHGSLTFYVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIATHEMA 187

Query: 561  NTYDRIFAVALIEHMKNYELFLKEVSKWMAQDGLLFVEHHCHKVFAYKYEPLDDDDWYTE 740
            +TYDRI  V L EHMKNY L LK++S+WMA+DGLLFV+H CHK FA+  EP+D+DDW +E
Sbjct: 188  DTYDRILVVGLFEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSE 247

Query: 741  YIFPTGTLVMSSSSILLYFQEDVSILNHWTLSGKHPSLGFKEWLKRLDDNMDAVKEIFES 920
            Y FP GT+++ S+S  LYFQ+D S++NHWTLSGKH S   +EWLKRLD N+  +  +F +
Sbjct: 248  YAFPAGTMIIPSASFFLYFQDDASVVNHWTLSGKHFSRTTEEWLKRLDANVKVIIPMFAT 307

Query: 921  FYGSKEKAMKWITYWRVFCIAHSEMYASNNGEEWMLSQVLFKK 1049
              GS+E+A+K + YWR  C    E+Y  NNGEEWM S VLFKK
Sbjct: 308  ITGSEEEAVKLLNYWRGLCFFGVELYGYNNGEEWMESHVLFKK 350