BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00000610
         (1114 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]       216   1e-53
gb|AFB74617.1| cytochrome P450 [Papaver somniferum]                   127   7e-27
ref|XP_002510313.1| cytochrome P450, putative [Ricinus communis]...   125   2e-26
ref|XP_003527704.1| PREDICTED: cytochrome P450 82A1-like [Glycin...   123   9e-26
gb|AAC39454.1| (S)-N-methylcoclaurine 3'-hydroxylase [Eschscholz...   122   1e-25

>dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 534

 Score =  216 bits (549), Expect = 1e-53
 Identities = 102/107 (95%), Positives = 103/107 (96%)
 Frame = +2

Query: 2   MIRTKKAPEVVGAWPVIGHLNLFSVPKPAYIVLGELADQYGPAFSIHFGIHPTLVVSSWE 181
           MIRTKKAPEVVGAWPVIGHLNL SVPKPAYIVLGELADQYGPAFSI FG+HP LVVSSWE
Sbjct: 39  MIRTKKAPEVVGAWPVIGHLNLLSVPKPAYIVLGELADQYGPAFSIQFGVHPILVVSSWE 98

Query: 182 LVKACLTTNDKFFSSRLVNKAIKYMFYDQKTISFAPHGPYWRELRKM 322
           LVKAC TTNDKFFSSRLVNKAIKYMFYDQKTISFAPHGPYWRELRKM
Sbjct: 99  LVKACFTTNDKFFSSRLVNKAIKYMFYDQKTISFAPHGPYWRELRKM 145



 Score =  210 bits (534), Expect = 6e-52
 Identities = 103/105 (98%), Positives = 103/105 (98%)
 Frame = +3

Query: 471 LYELSTKRKDENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPKTERYKNVMEEARRLMD 650
           LYELSTKRKDENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPKTERY NVMEEARRLMD
Sbjct: 174 LYELSTKRKDENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPKTERYMNVMEEARRLMD 233

Query: 651 VMVFSDVIPYLGWLDRLRGVDSEIKRTAKELDSVLESWVDEHRQK 785
           VMVFSDVIPYLGWLDRLRGVDSEIKRTAKELDS LESWVDEHRQK
Sbjct: 234 VMVFSDVIPYLGWLDRLRGVDSEIKRTAKELDSALESWVDEHRQK 278



 Score =  109 bits (273), Expect = 1e-21
 Identities = 53/53 (100%), Positives = 53/53 (100%)
 Frame = +3

Query: 954  IKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQCELDAQVGKERQVED 1112
            IKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQCELDAQVGKERQVED
Sbjct: 322  IKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQCELDAQVGKERQVED 374


>gb|AFB74617.1| cytochrome P450 [Papaver somniferum]
          Length = 556

 Score =  127 bits (318), Expect = 7e-27
 Identities = 63/108 (58%), Positives = 76/108 (70%), Gaps = 2/108 (1%)
 Frame = +2

Query: 5   IRTKKAPEVVGAWPVIGHLNLFSVPKPA-YIVLGELADQYGPAFSIHFGIHPTLVVSSWE 181
           I T   PE  GAWPVIGHL LF       ++ LG +AD+YGP FS+ FG H TLVVSSWE
Sbjct: 52  ITTTPLPEASGAWPVIGHLLLFMNENDLNHVTLGHMADKYGPIFSLRFGRHRTLVVSSWE 111

Query: 182 LVKACLT-TNDKFFSSRLVNKAIKYMFYDQKTISFAPHGPYWRELRKM 322
           +VK C T TNDK FS+R  + A+K MFYD ++  FAP+G YWRELRK+
Sbjct: 112 MVKECFTGTNDKLFSNRPSSLAVKLMFYDTESYGFAPYGKYWRELRKI 159



 Score = 77.4 bits (189), Expect = 6e-12
 Identities = 44/114 (38%), Positives = 64/114 (56%), Gaps = 9/114 (7%)
 Frame = +3

Query: 471 LYELSTKRKDEN----AGVLVDMSKWFGDISFNVVTRIVAG---KHIFGPKT--ERYKNV 623
           L+EL +  K          LV M  WF  ++FNV+ RIV+G     + G     E+YK  
Sbjct: 188 LHELCSNNKQGGDTTYVASLVRMDDWFAYLTFNVIGRIVSGFQSNAVAGATNSQEKYKLA 247

Query: 624 MEEARRLMDVMVFSDVIPYLGWLDRLRGVDSEIKRTAKELDSVLESWVDEHRQK 785
           ++E   LM     SDV+P LGW+DRL G+  ++K   K+LD+V+   V++HRQK
Sbjct: 248 IDEVSNLMATFAVSDVVPRLGWIDRLTGLTGKMKNCGKKLDAVVGDAVEDHRQK 301


>ref|XP_002510313.1| cytochrome P450, putative [Ricinus communis]
           gi|223551014|gb|EEF52500.1| cytochrome P450, putative
           [Ricinus communis]
          Length = 528

 Score =  125 bits (313), Expect = 2e-26
 Identities = 54/107 (50%), Positives = 77/107 (71%)
 Frame = +2

Query: 2   MIRTKKAPEVVGAWPVIGHLNLFSVPKPAYIVLGELADQYGPAFSIHFGIHPTLVVSSWE 181
           M + K APE  GAWP+IGHL+L   P+P++IV G +AD+YGP F+I  G+HPTLV S+WE
Sbjct: 32  MKKKKAAPEAGGAWPLIGHLHLLRGPQPSHIVFGNMADKYGPIFTIKMGVHPTLVASNWE 91

Query: 182 LVKACLTTNDKFFSSRLVNKAIKYMFYDQKTISFAPHGPYWRELRKM 322
           + K C TTNDK F++R    A+  + Y +   +F+P+G YWR++RK+
Sbjct: 92  MAKECFTTNDKAFANRPNILAMDLLGYGRSMFAFSPYGNYWRQIRKI 138



 Score = 78.2 bits (191), Expect = 3e-12
 Identities = 43/115 (37%), Positives = 68/115 (59%), Gaps = 10/115 (8%)
 Frame = +3

Query: 471 LYELSTKRKDENAG--VLVDMSKWFGDISFNVVTRIVAGKHIFGPKT--------ERYKN 620
           LY+L  K K  N+   VLV+M +WFGDI+ N++ RI+ GK I G +T        E +K 
Sbjct: 167 LYKLWEKNKTTNSNNKVLVEMKRWFGDITLNIILRIIVGKFI-GYETADEGKESNEGWKQ 225

Query: 621 VMEEARRLMDVMVFSDVIPYLGWLDRLRGVDSEIKRTAKELDSVLESWVDEHRQK 785
            + +   L    + +D +P+L WLD + G +  +K TA +LD V+  W++EH++K
Sbjct: 226 ALRDFFHLSGRFIAADAVPFLRWLD-IGGHEKTMKHTANKLDIVVTEWLNEHKEK 279



 Score = 62.0 bits (149), Expect = 3e-07
 Identities = 32/52 (61%), Positives = 38/52 (73%)
 Frame = +3

Query: 957  KAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQCELDAQVGKERQVED 1112
            KA    + LAA D T+VTL WAL LL+NN  VLK+AQ ELD QVG+ERQV +
Sbjct: 317  KATCLALTLAASDTTSVTLIWALSLLVNNPDVLKKAQDELDVQVGRERQVHE 368


>ref|XP_003527704.1| PREDICTED: cytochrome P450 82A1-like [Glycine max]
          Length = 535

 Score =  123 bits (308), Expect = 9e-26
 Identities = 52/103 (50%), Positives = 72/103 (69%)
 Frame = +2

Query: 14  KKAPEVVGAWPVIGHLNLFSVPKPAYIVLGELADQYGPAFSIHFGIHPTLVVSSWELVKA 193
           K  PE  GAWP+IGHL+LF   KP ++ LG +AD+YGP F++  G+H TLVVS+WE+ K 
Sbjct: 43  KSPPEASGAWPLIGHLHLFGASKPPHVTLGNMADKYGPIFTLRLGVHKTLVVSNWEMAKQ 102

Query: 194 CLTTNDKFFSSRLVNKAIKYMFYDQKTISFAPHGPYWRELRKM 322
           C T NDK F+SR  + A + + Y+   I F+P+G YWR +RK+
Sbjct: 103 CFTVNDKAFASRPKSVAFEVLGYNFSMIGFSPYGSYWRHVRKI 145



 Score = 72.8 bits (177), Expect = 1e-10
 Identities = 40/101 (39%), Positives = 57/101 (56%), Gaps = 3/101 (2%)
 Frame = +3

Query: 489 KRKDENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPKTERYKNVMEEARRLMDV---MV 659
           K K  +  V  +M +WFGDI   V+ R V GK  F  +TE  + + +  R L D+     
Sbjct: 181 KNKSGSEKVTTEMKRWFGDIMLKVMFRTVVGKR-FVLETEENERIRKAMRDLFDLSGSFS 239

Query: 660 FSDVIPYLGWLDRLRGVDSEIKRTAKELDSVLESWVDEHRQ 782
            SD +PYL W D L G + ++K TAKELD  +E W+ EH++
Sbjct: 240 VSDALPYLRWFD-LDGAEKKMKTTAKELDGFVEVWLQEHKR 279


>gb|AAC39454.1| (S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia californica]
          Length = 560

 Score =  122 bits (307), Expect = 1e-25
 Identities = 53/104 (50%), Positives = 73/104 (70%), Gaps = 1/104 (0%)
 Frame = +2

Query: 14  KKAPEVVGAWPVIGHL-NLFSVPKPAYIVLGELADQYGPAFSIHFGIHPTLVVSSWELVK 190
           K  PE  G+WP++GHL  L    KP + VLG++AD++GP F + FG++PTLVVS+WE+ K
Sbjct: 41  KHPPEAAGSWPIVGHLPQLVGSGKPLFRVLGDMADKFGPIFMVRFGVYPTLVVSTWEMAK 100

Query: 191 ACLTTNDKFFSSRLVNKAIKYMFYDQKTISFAPHGPYWRELRKM 322
            C T+NDKF +SR  + A  YM YD     F+ +GPYWRE+RK+
Sbjct: 101 ECFTSNDKFLASRPPSAASSYMTYDHAMFGFSFYGPYWREIRKI 144



 Score = 63.2 bits (152), Expect = 1e-07
 Identities = 32/53 (60%), Positives = 41/53 (77%)
 Frame = +3

Query: 954  IKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQCELDAQVGKERQVED 1112
            IKA    M +AA D T+V+LTWAL LLLNN QVL++AQ ELD +VG++R VE+
Sbjct: 344  IKATSLSMVVAASDTTSVSLTWALSLLLNNIQVLRKAQDELDTKVGRDRHVEE 396