BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00000481
         (1459 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]    214   e-108
gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]           211   e-104
ref|XP_002510298.1| cytochrome P450, putative [Ricinus communis]...   198   5e-99
ref|XP_003523888.1| PREDICTED: cytochrome P450 82C4-like [Glycin...   197   3e-97
ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif...   197   4e-97

>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
          Length = 524

 Score =  214 bits (545), Expect(2) = e-108
 Identities = 100/178 (56%), Positives = 139/178 (78%)
 Frame = +2

Query: 923  SKELDAILATWVEEHRHKKVSGSEDDEQDFIDLTLSMIDQTQHQGADVDTFIKSMCVGMI 1102
            +K++DAIL  W+EEHR KK S +E  +QDF+D+ LS++++++  G D D  IK+ C+ MI
Sbjct: 262  AKKVDAILENWLEEHRKKKNSVAES-QQDFMDVMLSIVEESKLSGHDADAVIKATCLAMI 320

Query: 1103 FGGSDSTSVALTWALSILMNNRPVLKKAREEIDRQVGTDRKVNDLDVLKLDHLKAIIKES 1282
             GG+D+T+V+LTW +S+LMNNR  LKKAREE+D  VG DR+V D D+  L ++ AI+KE+
Sbjct: 321  MGGTDTTAVSLTWIISLLMNNRHALKKAREELDALVGKDRQVEDSDLKNLVYMNAIVKET 380

Query: 1283 MRICLVGPLLERVTVEDCEIGGYHLKTGSRVIVNIWKMQHDPNLWPDPFEFRPERFLT 1456
            MR+  +G LLER T EDCEI G+H+K G+R++VN+WK+Q DPN+W DP EFRPERFLT
Sbjct: 381  MRMYPLGTLLERETKEDCEIDGFHVKGGTRLLVNVWKLQRDPNVWVDPTEFRPERFLT 438



 Score =  205 bits (522), Expect(2) = e-108
 Identities = 110/231 (47%), Positives = 149/231 (64%), Gaps = 2/231 (0%)
 Frame = +1

Query: 133 MDFTMLIQWLLTSLATLVAFVLIWYSATSSSTRKINK--TAPVAAGALPIVGHLHMLMGR 306
           MD  ML      S+A+++AFV + Y+  SS T K  K  TAP+A GA P++GHLH+    
Sbjct: 1   MDSLMLAYLFPISVASIIAFVFL-YNLFSSRTLKNKKIRTAPMATGAWPVLGHLHLFGSG 59

Query: 307 ELPHQLLSNMADKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAIFKRPRNKALKNLT 486
           ELPH++L+ MADKYG AF MK+G    LVVS  ++ KECF  ND     RP  KA + +T
Sbjct: 60  ELPHKMLAAMADKYGSAFRMKFGKHTTLVVSDTRIVKECFTTNDTLFSNRPSTKAFQLMT 119

Query: 487 YDQASFGFAPYGPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFIKELYDLWSSNSNN 666
           YD  S  F PYG  W E+RK++   LLSN RLQ  + VRASEV+   K LYD      N 
Sbjct: 120 YDNESVAFTPYGSYWREIRKISTLKLLSNHRLQAIKDVRASEVNVCFKTLYD---QCKNP 176

Query: 667 NKGAPLLVEMNKWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHYKKVV 819
           +  AP+L++M KWFEE++ NVV R++ G+++ GSK  + E+ E++HYKKV+
Sbjct: 177 SGSAPILIDMKKWFEEVSNNVVMRVIVGRQNFGSKIVQGEE-EAIHYKKVM 226


>gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]
          Length = 541

 Score =  211 bits (538), Expect(2) = e-104
 Identities = 103/177 (58%), Positives = 133/177 (75%)
 Frame = +2

Query: 926  KELDAILATWVEEHRHKKVSGSEDDEQDFIDLTLSMIDQTQHQGADVDTFIKSMCVGMIF 1105
            KEL++IL  W+EEH+ KK   S D  QDF+D+ LS+   TQ  G D DTFIK+ C+ MI 
Sbjct: 279  KELNSILQKWLEEHKSKK---SSDARQDFMDVMLSISKDTQLYGHDQDTFIKATCLAMIM 335

Query: 1106 GGSDSTSVALTWALSILMNNRPVLKKAREEIDRQVGTDRKVNDLDVLKLDHLKAIIKESM 1285
            GG++ST VALTW LS+LMNNR  L KAREEID  VG DR+V D DV  L ++ AIIKE+M
Sbjct: 336  GGTNSTEVALTWILSLLMNNRCALHKAREEIDLLVGKDRQVEDSDVKNLTYMNAIIKETM 395

Query: 1286 RICLVGPLLERVTVEDCEIGGYHLKTGSRVIVNIWKMQHDPNLWPDPFEFRPERFLT 1456
            R+  +G LLER T EDCE+ G+++K G+R+++N+WK+Q DPN+W DP EF+PERFLT
Sbjct: 396  RLYPLGFLLERDTKEDCEVSGFNIKGGTRLLINVWKLQRDPNVWTDPMEFKPERFLT 452



 Score =  196 bits (499), Expect(2) = e-104
 Identities = 102/251 (40%), Positives = 148/251 (58%), Gaps = 15/251 (5%)
 Frame = +1

Query: 133 MDFTMLIQWLLT------SLATLVAFVLIWY---------SATSSSTRKINKTAPVAAGA 267
           MDF+ L+  LL       S+A L+A VL++            TS   +KI    P   GA
Sbjct: 1   MDFSSLLLLLLNTWISAYSMAALLALVLVYNLRMTKSSSSKTTSLKGKKIITRPPAVTGA 60

Query: 268 LPIVGHLHMLMGRELPHQLLSNMADKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAI 447
            P+ GHLH+    E PH++LS +A+KYGP+F MK+G    LVVS  ++ KECF  ND   
Sbjct: 61  WPVFGHLHLFGSGEHPHEMLSKLAEKYGPSFTMKFGKHTTLVVSDTRVVKECFTTNDTLF 120

Query: 448 FKRPRNKALKNLTYDQASFGFAPYGPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFI 627
             RP   A   +TY   S  F PY P W E+RK++   LLSN RL+  + +R SEV    
Sbjct: 121 SNRPSTIAFDLMTYATDSIAFTPYSPYWRELRKISTLKLLSNNRLESIKQLRTSEVSVCF 180

Query: 628 KELYDLWSSNSNNNKGAPLLVEMNKWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHY 807
           KELYDL  +N  N+ GAP+ +++ +WF+E++ NV+ R++ GK++ GSK    ED E++HY
Sbjct: 181 KELYDL--TNKKNDNGAPVPIDLKRWFDEVSNNVIMRVIFGKQNFGSKIVLGEDQEAVHY 238

Query: 808 KKVVVDATLLT 840
           KK++ + + L+
Sbjct: 239 KKIMDELSRLS 249


>ref|XP_002510298.1| cytochrome P450, putative [Ricinus communis]
           gi|223550999|gb|EEF52485.1| cytochrome P450, putative
           [Ricinus communis]
          Length = 523

 Score =  198 bits (504), Expect(2) = 5e-99
 Identities = 101/223 (45%), Positives = 150/223 (67%), Gaps = 4/223 (1%)
 Frame = +1

Query: 163 LTSLATLVAFVLI---WYSATSSSTRKINKTAPVAAGALPIVGHLHMLMGRE-LPHQLLS 330
           LT++A +++F+ +   W      S    ++ AP  AG+ PI+GHLH+L G + L H+ L 
Sbjct: 7   LTAIAVIISFIFVCSTWLRGKKKSVGSKSREAPEPAGSWPIIGHLHLLGGADKLLHRTLG 66

Query: 331 NMADKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAIFKRPRNKALKNLTYDQASFGF 510
           ++ADKYGPAF ++ GS  ALVV+S +LAKECF  ND+ +  R    A K++ YD A FGF
Sbjct: 67  SLADKYGPAFNIRIGSHRALVVASKELAKECFTINDKTLASRSTTAATKHMCYDHAVFGF 126

Query: 511 APYGPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFIKELYDLWSSNSNNNKGAPLLV 690
           APY   W EMRK+    LLSN+RL+M +HV+ASEVD  I++LY+LW+     N+  P++V
Sbjct: 127 APYSSHWREMRKIVMLELLSNRRLEMVKHVQASEVDLGIRKLYNLWA----QNRCLPVIV 182

Query: 691 EMNKWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHYKKVV 819
           E+ ++FE+L L+V+ R+V+GKR+ GS    S+D E+  Y+K +
Sbjct: 183 ELKQFFEDLTLDVIVRVVAGKRYTGS----SDDDEARQYQKAI 221



 Score =  191 bits (484), Expect(2) = 5e-99
 Identities = 88/184 (47%), Positives = 135/184 (73%), Gaps = 4/184 (2%)
 Frame = +2

Query: 920  TSKELDAILATWVEEHRHKKVSGS--EDDEQDFIDLTLSMIDQTQHQGA--DVDTFIKSM 1087
            T+K+LDA+LA W++EHR ++VSG    + +QDFID+ LS+ ++    G   D DT IKS 
Sbjct: 255  TAKDLDAVLAGWLDEHRRRRVSGEVKSEGDQDFIDVMLSLEEKGHLSGFQYDADTSIKST 314

Query: 1088 CVGMIFGGSDSTSVALTWALSILMNNRPVLKKAREEIDRQVGTDRKVNDLDVLKLDHLKA 1267
            C+ +I G SD+T+  L WA+S+L+NN+  LK A+EE+++ +GT+R+V++ D+  L +L+A
Sbjct: 315  CLALIAGASDTTTTTLVWAISLLLNNQLALKNAKEELEKHIGTERQVDESDLKNLVYLQA 374

Query: 1268 IIKESMRICLVGPLLERVTVEDCEIGGYHLKTGSRVIVNIWKMQHDPNLWPDPFEFRPER 1447
            +IKE++R+  V PL+ R  VEDC +GGYH+  G+R++VN+WK+Q DP LW     F+PER
Sbjct: 375  VIKETLRLYPVAPLIPREFVEDCRVGGYHVPAGTRLLVNVWKIQRDPMLWTKASAFQPER 434

Query: 1448 FLTT 1459
            FLT+
Sbjct: 435  FLTS 438


>ref|XP_003523888.1| PREDICTED: cytochrome P450 82C4-like [Glycine max]
          Length = 526

 Score =  197 bits (501), Expect(2) = 3e-97
 Identities = 100/222 (45%), Positives = 149/222 (67%), Gaps = 3/222 (1%)
 Frame = +1

Query: 163 LTSLATLVAF-VLIWYSATS-SSTRKINKTAPVAAGALPIVGHLHMLMGRE-LPHQLLSN 333
           LT +A LV+  V +W++  +   ++  +K AP+ AGA P++GHLH+L G + L ++ L  
Sbjct: 7   LTIIAILVSLLVFLWHTKRNRGGSKNKSKEAPIPAGAWPLIGHLHLLGGDDQLLYRTLGT 66

Query: 334 MADKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAIFKRPRNKALKNLTYDQASFGFA 513
           MAD+YGPAF +  G++ A VVSS ++AKECF  ND+A+  RP   A K++ Y+ A FGFA
Sbjct: 67  MADQYGPAFNIWLGTRRAFVVSSWEVAKECFTSNDKALASRPTTVAAKHMGYNYAVFGFA 126

Query: 514 PYGPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFIKELYDLWSSNSNNNKGAPLLVE 693
           PY P W EMRK+A   LLSN+RL+M +HV  SE++  +++LY+ W      N+  P+LVE
Sbjct: 127 PYSPFWREMRKIATLELLSNRRLEMLKHVMVSELNMVMRDLYNSWV----QNRSRPVLVE 182

Query: 694 MNKWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHYKKVV 819
           +N+W E+L LN+V RMV+GKR+ G+ A    D E+   +K +
Sbjct: 183 LNRWLEDLTLNMVVRMVAGKRYFGASASCDNDDEARRCQKAI 224



 Score =  186 bits (472), Expect(2) = 3e-97
 Identities = 92/188 (48%), Positives = 132/188 (70%), Gaps = 8/188 (4%)
 Frame = +2

Query: 920  TSKELDAILATWVEEHRHKKVSGS--EDDEQDFIDLTLSM-----IDQTQHQGADVDTFI 1078
            T+KELDAIL  W++EHR ++V G    + EQDFID+ LS+     +   Q+   D DT I
Sbjct: 258  TAKELDAILEGWLKEHREQRVDGEIKAEGEQDFIDIMLSLQKGGHLSNFQY---DSDTSI 314

Query: 1079 KSMCVGMIFGGSDSTSVALTWALSILMNNRPVLKKAREEIDRQVGTDRKVNDLDVLKLDH 1258
            KS C+ +I GGSD+T+  +TWA+S+L+NNR  LKKA+EE+D  VG +R+V + D+  L +
Sbjct: 315  KSTCLALILGGSDTTAGTVTWAISLLLNNRQALKKAQEELDLNVGMERQVEESDIRNLAY 374

Query: 1259 LKAIIKESMRICLVGPLL-ERVTVEDCEIGGYHLKTGSRVIVNIWKMQHDPNLWPDPFEF 1435
            ++AIIKE++R+   GPLL  R   EDC + GYH+  G+R++VN+WK+  DP +W +P  F
Sbjct: 375  VQAIIKETLRLYPAGPLLGPREAQEDCNVAGYHVPAGTRLVVNLWKIHRDPRVWQEPSAF 434

Query: 1436 RPERFLTT 1459
            RPERFLT+
Sbjct: 435  RPERFLTS 442


>ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
           gi|302142392|emb|CBI19595.3| unnamed protein product
           [Vitis vinifera]
          Length = 527

 Score =  197 bits (501), Expect(2) = 4e-97
 Identities = 101/195 (51%), Positives = 137/195 (70%), Gaps = 1/195 (0%)
 Frame = +1

Query: 238 NKTAPVAAGALPIVGHLHMLMGRE-LPHQLLSNMADKYGPAFMMKYGSQPALVVSSLKLA 414
           ++ AP   GA PI+GHLH+L G + L ++ L  MADKYGPAF ++ GS+ A VVSS ++A
Sbjct: 35  SRDAPEPGGAWPIIGHLHLLGGGDQLLYRTLGAMADKYGPAFNIRLGSRRAFVVSSWEVA 94

Query: 415 KECFVHNDRAIFKRPRNKALKNLTYDQASFGFAPYGPLWVEMRKVAKTNLLSNQRLQMQR 594
           KECF  ND+A+  RP   A K++ Y+ A FGFAPY P W EMRK+A   LLSN+RL++ +
Sbjct: 95  KECFTINDKALATRPTTVAAKHMGYNYAVFGFAPYSPFWREMRKIATLELLSNRRLEILK 154

Query: 595 HVRASEVDAFIKELYDLWSSNSNNNKGAPLLVEMNKWFEELALNVVTRMVSGKRHIGSKA 774
           HVR SEVD  I+ELY LW  NS+     PLLVE+N+W E++ LNV+ RMV+GKR+ G+ A
Sbjct: 155 HVRTSEVDMGIRELYGLWVKNSSR----PLLVELNRWLEDMTLNVIVRMVAGKRYFGA-A 209

Query: 775 RRSEDSESMHYKKVV 819
             S+ SE+   +K +
Sbjct: 210 AASDSSEARRCQKAI 224



 Score =  186 bits (471), Expect(2) = 4e-97
 Identities = 93/183 (50%), Positives = 134/183 (73%), Gaps = 5/183 (2%)
 Frame = +2

Query: 920  TSKELDAILATWVEEHRHKKVSG--SEDDEQDFIDLTLSMIDQTQHQGA--DVDTFIKSM 1087
            T+KELD+IL  W+EEHR ++VS     + EQDFID+ LS+ ++ +  G   D +T IKS 
Sbjct: 258  TAKELDSILEGWLEEHRQRRVSSLIKAEGEQDFIDVMLSLQEEGRLSGFQYDSETSIKST 317

Query: 1088 CVGMIFGGSDSTSVALTWALSILMNNRPVLKKAREEIDRQVGTDRKVNDLDVLKLDHLKA 1267
            C+ +I GGSD+T+  LTWA+S+L+NNR  LKKA+EE+D  VG +R+V + DV  L +L+A
Sbjct: 318  CLALILGGSDTTAGTLTWAISLLLNNRHALKKAQEELDLCVGMERQVEESDVKNLVYLQA 377

Query: 1268 IIKESMRICLVGPLL-ERVTVEDCEIGGYHLKTGSRVIVNIWKMQHDPNLWPDPFEFRPE 1444
            IIKE++R+   GPLL  R  ++DC + GY++  G+R+IVNIWK+Q DP++W +P  F+PE
Sbjct: 378  IIKETLRLYPAGPLLGPREALDDCTVAGYNVPAGTRLIVNIWKLQRDPSVWTNPCAFQPE 437

Query: 1445 RFL 1453
            RFL
Sbjct: 438  RFL 440