BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00033666
         (918 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281545.1| PREDICTED: probable E3 ubiquitin-protein lig...   292   8e-77
emb|CAN65617.1| hypothetical protein VITISV_032298 [Vitis vinifera]   290   2e-76
ref|XP_002525919.1| Protein pim1, putative [Ricinus communis] gi...   285   1e-74
ref|XP_002330952.1| predicted protein [Populus trichocarpa] gi|2...   284   2e-74
ref|XP_004156169.1| PREDICTED: probable E3 ubiquitin-protein lig...   280   3e-73

>ref|XP_002281545.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1 [Vitis
           vinifera] gi|298204453|emb|CBI16933.3| unnamed protein
           product [Vitis vinifera]
          Length = 478

 Score =  292 bits (747), Expect = 8e-77
 Identities = 133/168 (79%), Positives = 146/168 (86%)
 Frame = -2

Query: 581 MGVLDVLGETEQLVNNVPTKSAIYVWGYNQSGQTGRKCKEFHLRIPKHLPPELFGCPGGV 402
           M + D+ GE+  +  N+PTKSAIYVWGYNQ GQTGR  KE  LRIPK LPPELFGCP G 
Sbjct: 1   MDIGDIFGESRPV--NIPTKSAIYVWGYNQRGQTGRNGKELQLRIPKQLPPELFGCPAGA 58

Query: 401 NARWLDIACGREHTAAVASDGSLFTWGANDFGQLGDGTEDGRKRPQKVNQFQTEFVKSVS 222
           N+RWL IACGREHTAAVASDGSLFTWGANDFGQLGDGTEDGRK P+KV Q QTEFVKS+S
Sbjct: 59  NSRWLSIACGREHTAAVASDGSLFTWGANDFGQLGDGTEDGRKHPKKVKQLQTEFVKSIS 118

Query: 221 CGAHCTAAISEPRENDGSLSTSRLWVWGQNQGSNYPRLYWGAFTPNTV 78
           CGAHCTAAI+EPRE +G +STSRLWVWGQNQGSNYP L+WGAFTPNT+
Sbjct: 119 CGAHCTAAIAEPREKNGIVSTSRLWVWGQNQGSNYPHLFWGAFTPNTI 166



 Score = 60.1 bits (144), Expect = 7e-07
 Identities = 40/129 (31%), Positives = 59/129 (45%), Gaps = 9/129 (6%)
 Frame = -2

Query: 539 NNVPTKSAIYVWGYNQSGQTGRKCKEFHLRIPKHLPPELFGCPGGVNARWLDIACGREHT 360
           N + + S ++VWG NQ                    P LF      N     ++CG  HT
Sbjct: 134 NGIVSTSRLWVWGQNQGSNY----------------PHLFWGAFTPNTIIRQVSCGAVHT 177

Query: 359 AAVASDGSLFTWGANDFGQLGDG-TEDGRKRPQKVNQFQTEF--------VKSVSCGAHC 207
            A++ +G +  WG N++GQLG G T +G +  + +N +   F        +  VSCG + 
Sbjct: 178 VALSEEGLVQAWGYNEYGQLGRGITCEGLQGARVINAYSKFFNEAPELVKITQVSCGEYH 237

Query: 206 TAAISEPRE 180
           TAAISE  E
Sbjct: 238 TAAISENGE 246


>emb|CAN65617.1| hypothetical protein VITISV_032298 [Vitis vinifera]
          Length = 461

 Score =  290 bits (743), Expect = 2e-76
 Identities = 133/168 (79%), Positives = 145/168 (86%)
 Frame = -2

Query: 581 MGVLDVLGETEQLVNNVPTKSAIYVWGYNQSGQTGRKCKEFHLRIPKHLPPELFGCPGGV 402
           M + D+ GE+  +  N+PTKSAIYVWGYNQ GQTGR  KE  LRIPK LPPELFGCP G 
Sbjct: 1   MDIGDIFGESRPV--NIPTKSAIYVWGYNQRGQTGRNGKELQLRIPKQLPPELFGCPAGA 58

Query: 401 NARWLDIACGREHTAAVASDGSLFTWGANDFGQLGDGTEDGRKRPQKVNQFQTEFVKSVS 222
           N+RWL IACGREHTAAVASDGSLFTWGANDFGQLGDGTEDGRK P+KV Q QTEFVKS S
Sbjct: 59  NSRWLSIACGREHTAAVASDGSLFTWGANDFGQLGDGTEDGRKHPKKVKQLQTEFVKSXS 118

Query: 221 CGAHCTAAISEPRENDGSLSTSRLWVWGQNQGSNYPRLYWGAFTPNTV 78
           CGAHCTAAI+EPRE +G +STSRLWVWGQNQGSNYP L+WGAFTPNT+
Sbjct: 119 CGAHCTAAIAEPREKNGIVSTSRLWVWGQNQGSNYPHLFWGAFTPNTI 166



 Score = 59.3 bits (142), Expect = 1e-06
 Identities = 42/129 (32%), Positives = 60/129 (46%), Gaps = 9/129 (6%)
 Frame = -2

Query: 539 NNVPTKSAIYVWGYNQSGQTGRKCKEFHLRIPKHLPPELFGCPGGVNARWLDIACGREHT 360
           N + + S ++VWG NQ                    P LF      N     ++CG  HT
Sbjct: 134 NGIVSTSRLWVWGQNQGSNY----------------PHLFWGAFTPNTIIRQVSCGAVHT 177

Query: 359 AAVASDGSLFTWGANDFGQLGDG-TEDGRKRPQKVNQFQ------TEFVK--SVSCGAHC 207
            A++ +G +  WG N++GQLG G T +G +  + +N +        E VK   VSCG + 
Sbjct: 178 VALSEEGLVQAWGYNEYGQLGRGITCEGLQGARVINAYSKFLNEAPELVKITQVSCGEYH 237

Query: 206 TAAISEPRE 180
           TAAISE  E
Sbjct: 238 TAAISENGE 246



 Score = 58.9 bits (141), Expect = 2e-06
 Identities = 41/135 (30%), Positives = 62/135 (45%), Gaps = 6/135 (4%)
 Frame = -2

Query: 515 IYVWGYNQSGQTGR--KCKEFH-LRIPKHLPPELFGCPGGVNARWLDIACGREHTAAVAS 345
           +  WGYN+ GQ GR   C+     R+       L   P  V      ++CG  HTAA++ 
Sbjct: 186 VQAWGYNEYGQLGRGITCEGLQGARVINAYSKFLNEAPELVKIT--QVSCGEYHTAAISE 243

Query: 344 DGSLFTWGANDFGQLGD-GTEDGRKR--PQKVNQFQTEFVKSVSCGAHCTAAISEPREND 174
           +G ++TWG  + GQLG    + G K   P++V       +K ++CG   T A++      
Sbjct: 244 NGEVYTWGLGNMGQLGHCSLQYGDKELVPRRVVALDGISIKDIACGGVHTCALT------ 297

Query: 173 GSLSTSRLWVWGQNQ 129
              S   L+ WG  Q
Sbjct: 298 ---SKGALYAWGGGQ 309


>ref|XP_002525919.1| Protein pim1, putative [Ricinus communis]
           gi|223534748|gb|EEF36439.1| Protein pim1, putative
           [Ricinus communis]
          Length = 478

 Score =  285 bits (728), Expect = 1e-74
 Identities = 131/168 (77%), Positives = 147/168 (87%)
 Frame = -2

Query: 581 MGVLDVLGETEQLVNNVPTKSAIYVWGYNQSGQTGRKCKEFHLRIPKHLPPELFGCPGGV 402
           M + +++GET     N+PTKSAIYVWGYN +GQTGRK KE  LRIPK L PELFGCP G 
Sbjct: 1   MDIDEIIGETTTRSMNLPTKSAIYVWGYNHNGQTGRKGKEQELRIPKQLSPELFGCPAGA 60

Query: 401 NARWLDIACGREHTAAVASDGSLFTWGANDFGQLGDGTEDGRKRPQKVNQFQTEFVKSVS 222
           NARWLDIACGREHTAAVASDGSLFTWGAN+FGQLGDGTE+G+K P+KV Q +TEFVKSVS
Sbjct: 61  NARWLDIACGREHTAAVASDGSLFTWGANEFGQLGDGTEEGKKYPKKVKQLETEFVKSVS 120

Query: 221 CGAHCTAAISEPRENDGSLSTSRLWVWGQNQGSNYPRLYWGAFTPNTV 78
           CGAHCTAAI+EPRENDG++STSRLWVWGQNQGSNYPRL+ GAF  NT+
Sbjct: 121 CGAHCTAAIAEPRENDGTISTSRLWVWGQNQGSNYPRLFSGAFALNTI 168



 Score = 65.5 bits (158), Expect = 2e-08
 Identities = 50/161 (31%), Positives = 71/161 (44%), Gaps = 10/161 (6%)
 Frame = -2

Query: 632 YPKTIEN*SSLFIYLKKMGVLDVLGETEQLVNN-VPTKSAIYVWGYNQSGQTGRKCKEFH 456
           YPK ++   + F+     G        E   N+   + S ++VWG NQ            
Sbjct: 104 YPKKVKQLETEFVKSVSCGAHCTAAIAEPRENDGTISTSRLWVWGQNQGSNY-------- 155

Query: 455 LRIPKHLPPELFGCPGGVNARWLDIACGREHTAAVASDGSLFTWGANDFGQLGDG-TEDG 279
                   P LF     +N     ++CG  H  A++ DG L  WG N++GQLG G T +G
Sbjct: 156 --------PRLFSGAFALNTIICQVSCGAVHVVALSEDGLLQAWGYNEYGQLGRGITCEG 207

Query: 278 RKRPQKVNQF------QTEFVK--SVSCGAHCTAAISEPRE 180
            + P+ +N +        E VK   VSCG + TAAISE  E
Sbjct: 208 LQGPRVINAYAKFLDEAPELVKITRVSCGEYHTAAISEKGE 248



 Score = 59.3 bits (142), Expect = 1e-06
 Identities = 46/168 (27%), Positives = 73/168 (43%), Gaps = 19/168 (11%)
 Frame = -2

Query: 515 IYVWGYNQSGQTGRKCKEFHLRIPKHLPPELFGCPGGVNARWLD----------IACGRE 366
           +  WGYN+ GQ GR      L+ P+ +            A++LD          ++CG  
Sbjct: 188 LQAWGYNEYGQLGRGITCEGLQGPRVI---------NAYAKFLDEAPELVKITRVSCGEY 238

Query: 365 HTAAVASDGSLFTWGANDFGQLG-DGTEDGRKR--PQKVNQFQTEFVKSVSCGAHCTAAI 195
           HTAA++  G ++TWG    GQLG    + G K   P++V       +  V+CG   T A+
Sbjct: 239 HTAAISEKGEVYTWGLGSTGQLGHTSLQSGNKELLPRRVVALDGILITDVACGGVHTCAV 298

Query: 194 SEPRENDGSLSTSRLWVWGQNQGSNYPRLYWG------AFTPNTVHNF 69
           ++            L+ WG   GS + +L  G      +F P+   +F
Sbjct: 299 TQ---------KGALYAWG---GSQFGQLGLGPQTGSFSFIPSDTESF 334


>ref|XP_002330952.1| predicted protein [Populus trichocarpa] gi|222873146|gb|EEF10277.1|
           predicted protein [Populus trichocarpa]
          Length = 462

 Score =  284 bits (727), Expect = 2e-74
 Identities = 133/168 (79%), Positives = 150/168 (89%)
 Frame = -2

Query: 581 MGVLDVLGETEQLVNNVPTKSAIYVWGYNQSGQTGRKCKEFHLRIPKHLPPELFGCPGGV 402
           M + +++GET++ VN  PTKSAIYVWGYNQSGQTGRK K+  LRIPK LPPELFGCP G 
Sbjct: 1   MDINEIVGETKRSVN-FPTKSAIYVWGYNQSGQTGRKGKDGQLRIPKQLPPELFGCPAGA 59

Query: 401 NARWLDIACGREHTAAVASDGSLFTWGANDFGQLGDGTEDGRKRPQKVNQFQTEFVKSVS 222
           NARWLDIACGREHTAAVASDGSLFTWGAN+FGQLGDGTED RK P+KV Q +TEFVKSVS
Sbjct: 60  NARWLDIACGREHTAAVASDGSLFTWGANEFGQLGDGTEDSRKYPKKVKQLETEFVKSVS 119

Query: 221 CGAHCTAAISEPRENDGSLSTSRLWVWGQNQGSNYPRLYWGAFTPNTV 78
           CGAHCTAAI+EPR+NDG++  SRLWVWGQNQGSNYPRL+ GAF+PNT+
Sbjct: 120 CGAHCTAAIAEPRQNDGTI-LSRLWVWGQNQGSNYPRLFCGAFSPNTI 166



 Score = 63.5 bits (153), Expect = 6e-08
 Identities = 49/160 (30%), Positives = 68/160 (42%), Gaps = 9/160 (5%)
 Frame = -2

Query: 632 YPKTIEN*SSLFIYLKKMGVLDVLGETEQLVNNVPTKSAIYVWGYNQSGQTGRKCKEFHL 453
           YPK ++   + F+     G        E   N+    S ++VWG NQ             
Sbjct: 103 YPKKVKQLETEFVKSVSCGAHCTAAIAEPRQNDGTILSRLWVWGQNQGSNY--------- 153

Query: 452 RIPKHLPPELFGCPGGVNARWLDIACGREHTAAVASDGSLFTWGANDFGQLGDG-TEDGR 276
                  P LF      N     ++CG  H  A++ DG L  WG N++GQLG G T +G 
Sbjct: 154 -------PRLFCGAFSPNTIICQVSCGAVHVVALSEDGLLQAWGYNEYGQLGRGFTCEGL 206

Query: 275 KRPQKVNQF------QTEFVK--SVSCGAHCTAAISEPRE 180
           +  + +N +        E VK   VSCG + TAAISE  E
Sbjct: 207 QGARVINAYAKFLDEAPELVKITQVSCGEYHTAAISEKGE 246



 Score = 60.8 bits (146), Expect = 4e-07
 Identities = 46/158 (29%), Positives = 69/158 (43%), Gaps = 9/158 (5%)
 Frame = -2

Query: 515 IYVWGYNQSGQTGR--KCKEFH-LRIPKHLPPELFGCPGGVNARWLDIACGREHTAAVAS 345
           +  WGYN+ GQ GR   C+     R+       L   P  V      ++CG  HTAA++ 
Sbjct: 186 LQAWGYNEYGQLGRGFTCEGLQGARVINAYAKFLDEAPELVKIT--QVSCGEYHTAAISE 243

Query: 344 DGSLFTWGANDFGQLG-DGTEDGRKR--PQKVNQFQTEFVKSVSCGAHCTAAISEPREND 174
            G ++TWG  + GQLG    + G K   P++V      F+K V+CG   T A ++     
Sbjct: 244 KGEVYTWGLGNMGQLGHTSLQSGDKELLPRRVVALDGIFIKDVACGGVHTCAATQ----- 298

Query: 173 GSLSTSRLWVWGQNQGSNY---PRLYWGAFTPNTVHNF 69
                  L+ WG  Q       P+    +F P+   +F
Sbjct: 299 ----KGALYAWGGGQAGQLGLGPQTGSFSFIPSESESF 332


>ref|XP_004156169.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC4-like isoform
           2 [Cucumis sativus]
          Length = 472

 Score =  280 bits (717), Expect = 3e-73
 Identities = 127/168 (75%), Positives = 147/168 (87%)
 Frame = -2

Query: 581 MGVLDVLGETEQLVNNVPTKSAIYVWGYNQSGQTGRKCKEFHLRIPKHLPPELFGCPGGV 402
           M + D+ G T+ +  ++P KSAIYVWGYNQSGQTGRK K+  LR+P+ L P+LFGCPG +
Sbjct: 1   MDIDDIFGMTQNV--SLPRKSAIYVWGYNQSGQTGRKGKDHQLRVPRQLHPDLFGCPGEI 58

Query: 401 NARWLDIACGREHTAAVASDGSLFTWGANDFGQLGDGTEDGRKRPQKVNQFQTEFVKSVS 222
           N+RWLDIACGREHTAAVASDGSLFTWGANDFGQLGDGTE+  K P+KVNQ Q EFVKSVS
Sbjct: 59  NSRWLDIACGREHTAAVASDGSLFTWGANDFGQLGDGTEEKSKLPKKVNQLQGEFVKSVS 118

Query: 221 CGAHCTAAISEPRENDGSLSTSRLWVWGQNQGSNYPRLYWGAFTPNTV 78
           CGAHCTAAI+EPRENDG+LST RLW+WGQNQGSN+PRL+WGAFTP T+
Sbjct: 119 CGAHCTAAIAEPRENDGTLSTRRLWIWGQNQGSNFPRLFWGAFTPQTI 166



 Score = 58.5 bits (140), Expect = 2e-06
 Identities = 43/135 (31%), Positives = 62/135 (45%), Gaps = 6/135 (4%)
 Frame = -2

Query: 515 IYVWGYNQSGQTGRKCKEFHL---RIPKHLPPELFGCPGGVNARWLDIACGREHTAAVAS 345
           +  WGYN+ GQ GR      L   RI       L   P  V      ++CG  H+AA++ 
Sbjct: 186 LQAWGYNEYGQLGRGVTSEGLQGARIINAFAKFLDEAPELVTIT--KVSCGEYHSAAISE 243

Query: 344 DGSLFTWGANDFGQLGD---GTEDGRKRPQKVNQFQTEFVKSVSCGAHCTAAISEPREND 174
           +G ++TWG    GQLG     + D    P++V       VK ++CG   T AI++    +
Sbjct: 244 NGEVYTWGLGSMGQLGHCSLQSADKELIPRRVVALDGICVKDIACGGVHTCAITQ----N 299

Query: 173 GSLSTSRLWVWGQNQ 129
           GS     L+ WG  Q
Sbjct: 300 GS-----LYAWGGGQ 309