BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00028248
         (566 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      295   5e-78
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   286   2e-75
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       270   9e-71
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   238   4e-61
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   222   3e-56

>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  295 bits (754), Expect = 5e-78
 Identities = 132/152 (86%), Positives = 144/152 (94%)
 Frame = +3

Query: 3   EHMKNYELLLRKISEWMIPDGLLFIEHVCHKTFAYHYEPLDEDDWFTEYVFPAGTVIIPS 182
           EHMKNYELLLRKISEW+  DGLLF+EH+CHKTFAYHYEPLD+DDWFTEYVFPAGT+IIPS
Sbjct: 207 EHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVFPAGTMIIPS 266

Query: 183 ASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDANIDAIKPIFETLTGSAEEAVKLI 362
           ASF LYFQDDVSVVNHWTLSGKH+SRTNEEWLKRLDAN+D IKP+FETL G+ EEAVKLI
Sbjct: 267 ASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVKLI 326

Query: 363 NYWRGFCLSGIELCGYNNGEEWMESHVLFKRK 458
           NYWRGFCLSG+E+ GYNNGEEWM SHVLFK+K
Sbjct: 327 NYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 358


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
           gi|51980204|gb|AAU20766.1| (S)-coclaurine
           N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  286 bits (732), Expect = 2e-75
 Identities = 128/153 (83%), Positives = 142/153 (92%), Gaps = 1/153 (0%)
 Frame = +3

Query: 3   EHMKNYELLLRKISEWMIPDGLLFIEHVCHKTFAYHYEPLDEDDWFTEYVFPAGTVIIPS 182
           EHMKNYELLLRKI EWM  DGLLF+EH+CHKTFAYHYEP+DEDDWFTEYVFPAGT+IIPS
Sbjct: 209 EHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPIDEDDWFTEYVFPAGTMIIPS 268

Query: 183 ASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDANIDAIKPIFETLTGSA-EEAVKL 359
           ASF LYFQDDVSVVNHWTLSGKH+SRTNEEWLKRLDAN++ IKP+F T+TG   +EA+KL
Sbjct: 269 ASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANVELIKPMFVTITGQCRQEAMKL 328

Query: 360 INYWRGFCLSGIELCGYNNGEEWMESHVLFKRK 458
           INYWRGFCLSG+E+ GYNNGEEWM SHVLFK+K
Sbjct: 329 INYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 361


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  270 bits (691), Expect = 9e-71
 Identities = 120/152 (78%), Positives = 134/152 (88%)
 Frame = +3

Query: 3   EHMKNYELLLRKISEWMIPDGLLFIEHVCHKTFAYHYEPLDEDDWFTEYVFPAGTVIIPS 182
           EHMKNY LLL+KISEWM  DGLLF++HVCHKTFA+H EP+DEDDW +EY FPAGT+IIPS
Sbjct: 200 EHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSEYAFPAGTMIIPS 259

Query: 183 ASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDANIDAIKPIFETLTGSAEEAVKLI 362
           ASF LYFQDD SVVNHWTLSGKH+SRT EEWLKRLDAN+  I P+F T+TGS EEAVKL+
Sbjct: 260 ASFFLYFQDDASVVNHWTLSGKHFSRTTEEWLKRLDANVKVIIPMFATITGSEEEAVKLL 319

Query: 363 NYWRGFCLSGIELCGYNNGEEWMESHVLFKRK 458
           NYWRG C  G+EL GYNNGEEWMESHVLFK+K
Sbjct: 320 NYWRGLCFFGVELYGYNNGEEWMESHVLFKKK 351


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
           somniferum]
          Length = 351

 Score =  238 bits (608), Expect = 4e-61
 Identities = 104/152 (68%), Positives = 127/152 (83%)
 Frame = +3

Query: 3   EHMKNYELLLRKISEWMIPDGLLFIEHVCHKTFAYHYEPLDEDDWFTEYVFPAGTVIIPS 182
           EHMKNY+LLL+KISEWM  DGLLF+EHVCHKT AYHYEP+D +DW+T Y+FPAGT+ + S
Sbjct: 200 EHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTNYIFPAGTLTLSS 259

Query: 183 ASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDANIDAIKPIFETLTGSAEEAVKLI 362
           AS LLYFQDDVSVVN WTLSGKHYSR++EEWLK +D NI   K I  ++T + +EA+KL+
Sbjct: 260 ASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKEIMRSITKTEKEAIKLL 319

Query: 363 NYWRGFCLSGIELCGYNNGEEWMESHVLFKRK 458
           N+WR FC+ G EL GY NGEEWM +H+LFK+K
Sbjct: 320 NFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 351


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
           gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
           [Thalictrum flavum]
          Length = 356

 Score =  222 bits (566), Expect = 3e-56
 Identities = 99/152 (65%), Positives = 122/152 (80%)
 Frame = +3

Query: 3   EHMKNYELLLRKISEWMIPDGLLFIEHVCHKTFAYHYEPLDEDDWFTEYVFPAGTVIIPS 182
           EHMKNYEL L+K+S W+   GLLF+EH CHK FAY YEPLDEDDW+TEY+FP+GT+++ S
Sbjct: 205 EHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQYEPLDEDDWYTEYIFPSGTLVMSS 264

Query: 183 ASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDANIDAIKPIFETLTGSAEEAVKLI 362
           +S LLYFQ+DVSVVNHWTLSGKH S   ++WLKRLD NID +K IFE+  GS E+A+K I
Sbjct: 265 SSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEIFESFYGSKEKAMKFI 324

Query: 363 NYWRGFCLSGIELCGYNNGEEWMESHVLFKRK 458
            YWR FC++  ++   NNGEEWM S VLFK+K
Sbjct: 325 TYWRVFCIAHSQMYSTNNGEEWMLSQVLFKKK 356