BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00027626
         (955 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002321248.1| cytochrome P450 probable ent-kaurenoic acid ...   485   e-135
ref|XP_002526524.1| Ent-kaurenoic acid oxidase, putative [Ricinu...   468   e-129
ref|XP_002879389.1| ent-kaurenoic acid hydroxylase [Arabidopsis ...   466   e-129
ref|XP_002892270.1| ent-kaurenoic acid hydroxylase 1 [Arabidopsi...   466   e-129
ref|XP_002319447.1| cytochrome P450 probable ent-kaurenoic acid ...   462   e-128

>ref|XP_002321248.1| cytochrome P450 probable ent-kaurenoic acid oxidase [Populus
            trichocarpa] gi|222862021|gb|EEE99563.1| cytochrome P450
            probable ent-kaurenoic acid oxidase [Populus trichocarpa]
          Length = 493

 Score =  485 bits (1248), Expect = e-135
 Identities = 233/319 (73%), Positives = 274/319 (85%), Gaps = 1/319 (0%)
 Frame = -1

Query: 955  VNGHEALSVYLEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIIMHIFISKGGESVMDA 776
            VNGHEALSVY+ +IEENV++ LE+W+ MG+IEFLTQ+RKLTF+IIM+IF+S   E VM+A
Sbjct: 149  VNGHEALSVYIPYIEENVIAMLEKWTTMGKIEFLTQVRKLTFKIIMYIFLSSESEVVMEA 208

Query: 775  LEEVYTTLNYGVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPAKK-D 599
            LE+ YTTLNYGVRAMAINLPGFAYY+ALK+RKRLV IF S+V+ERR +R      AKK D
Sbjct: 209  LEKDYTTLNYGVRAMAINLPGFAYYKALKARKRLVAIFQSIVDERRNLRKNSARNAKKKD 268

Query: 598  MLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKAKAE 419
            M+D+LL V+DEN RKL DEEIID ++MYLNAGHESSGHIT WAT+ LQEHP+  QKAK E
Sbjct: 269  MMDSLLGVEDENGRKLTDEEIIDVILMYLNAGHESSGHITTWATIFLQEHPEFLQKAKEE 328

Query: 418  QEQIVKNRPSMQKGLRLKEIRQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYIIPM 239
            QEQIVK RP  Q GL LKE+R+MDYL+KVIDETLRLI+FSL VFREAK D S+NGYIIP 
Sbjct: 329  QEQIVKRRPPAQNGLSLKEVREMDYLSKVIDETLRLITFSLTVFREAKTDFSINGYIIPK 388

Query: 238  GWKVEVWLRNVHRDPEVYPDPNNFDPSRWDGFTPKAGTYLPFGAGSRLCPGNELAKLEIA 59
            GWKV VW R VH DPE+Y +P  F+PSRWD +TPKAGT+LPFGAGSRLCPGN+LAKLEI+
Sbjct: 389  GWKVLVWFRTVHLDPEIYQNPKEFNPSRWDNYTPKAGTFLPFGAGSRLCPGNDLAKLEIS 448

Query: 58   IFMHYFLLNYRVERLNPKC 2
            IF+HYFLL+YR+ER NP+C
Sbjct: 449  IFLHYFLLDYRLERENPEC 467


>ref|XP_002526524.1| Ent-kaurenoic acid oxidase, putative [Ricinus communis]
            gi|223534199|gb|EEF35915.1| Ent-kaurenoic acid oxidase,
            putative [Ricinus communis]
          Length = 492

 Score =  468 bits (1203), Expect = e-129
 Identities = 225/318 (70%), Positives = 268/318 (84%), Gaps = 2/318 (0%)
 Frame = -1

Query: 955  VNGHEALSVYLEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIIMHIFISKGGESVMDA 776
            VNGHEALSVY+ +IE+ V S LE+WS MGEI+FLTQLRKLTFRIIM+IF+S    SVM+A
Sbjct: 149  VNGHEALSVYMHYIEDKVKSALEKWSTMGEIQFLTQLRKLTFRIIMYIFLSSESHSVMEA 208

Query: 775  LEEVYTTLNYGVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPA--KK 602
            LE  YTTLNYGVRAMAINLPGFAYY+ALK+RK LV +   VV+ RR  +    P +  KK
Sbjct: 209  LEREYTTLNYGVRAMAINLPGFAYYKALKARKNLVAVLQFVVDARRNQKKGAEPNSSKKK 268

Query: 601  DMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKAKA 422
            DM+D LLDV+DE  RKL+DEEI+D L+MYLNAGHESSGHITMWATV LQEHP+  QKA+ 
Sbjct: 269  DMMDALLDVEDEKGRKLSDEEIVDVLLMYLNAGHESSGHITMWATVFLQEHPEFLQKARQ 328

Query: 421  EQEQIVKNRPSMQKGLRLKEIRQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYIIP 242
            EQE+I+K RP  QKGL LKE+R M+YL+KVIDETLRLI+FSLVVFREAK +V+++GY+IP
Sbjct: 329  EQEEIIKRRPPTQKGLTLKEVRDMEYLSKVIDETLRLITFSLVVFREAKTNVNISGYVIP 388

Query: 241  MGWKVEVWLRNVHRDPEVYPDPNNFDPSRWDGFTPKAGTYLPFGAGSRLCPGNELAKLEI 62
             GWK+ VW R+VH DPE+YP+P  F+PSRWD  T KAGT+LPFGAGSR+CPGN+LAKLEI
Sbjct: 389  KGWKILVWFRSVHLDPEIYPNPREFNPSRWDNHTAKAGTFLPFGAGSRMCPGNDLAKLEI 448

Query: 61   AIFMHYFLLNYRVERLNP 8
            AIF+H+FLLNY +ERLNP
Sbjct: 449  AIFLHHFLLNYELERLNP 466


>ref|XP_002879389.1| ent-kaurenoic acid hydroxylase [Arabidopsis lyrata subsp. lyrata]
            gi|297325228|gb|EFH55648.1| ent-kaurenoic acid
            hydroxylase [Arabidopsis lyrata subsp. lyrata]
          Length = 489

 Score =  466 bits (1199), Expect = e-129
 Identities = 224/318 (70%), Positives = 266/318 (83%)
 Frame = -1

Query: 955  VNGHEALSVYLEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIIMHIFISKGGESVMDA 776
            VNG EALSVY++FIEE V++ LE+WS MGEIEFL+ LRKLTF++IM+IF+S   E VMDA
Sbjct: 150  VNGPEALSVYIQFIEETVITDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLSSESEHVMDA 209

Query: 775  LEEVYTTLNYGVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPAKKDM 596
            LE  YT LNYGVRAM INLPGFAY+RALK+RK+LV  F S+V  RR  R ++    +KDM
Sbjct: 210  LEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQNISSNRKDM 269

Query: 595  LDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKAKAEQ 416
            LDNL+DVKDEN R L+DEEIID L+MYLNAGHESSGH+TMWAT+L+QEHP++ QKAK EQ
Sbjct: 270  LDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPEILQKAKEEQ 329

Query: 415  EQIVKNRPSMQKGLRLKEIRQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYIIPMG 236
            E+IVKNR   QK L LKE R+M+YL++VIDETLR+I+FSL  FREAK DV ++GYIIP G
Sbjct: 330  ERIVKNRALGQK-LTLKETREMEYLSQVIDETLRVITFSLTAFREAKSDVQIDGYIIPKG 388

Query: 235  WKVEVWLRNVHRDPEVYPDPNNFDPSRWDGFTPKAGTYLPFGAGSRLCPGNELAKLEIAI 56
            WKV  W RNVH DPE+YPDP  FDPSRW+G+TPKAGT+LPFG GS LCPGN+LAKLEI+I
Sbjct: 389  WKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGNDLAKLEISI 448

Query: 55   FMHYFLLNYRVERLNPKC 2
            F+H+FLL YRVER NP C
Sbjct: 449  FLHHFLLRYRVERSNPGC 466


>ref|XP_002892270.1| ent-kaurenoic acid hydroxylase 1 [Arabidopsis lyrata subsp. lyrata]
            gi|297338112|gb|EFH68529.1| ent-kaurenoic acid
            hydroxylase 1 [Arabidopsis lyrata subsp. lyrata]
          Length = 491

 Score =  466 bits (1198), Expect = e-129
 Identities = 224/320 (70%), Positives = 265/320 (82%), Gaps = 2/320 (0%)
 Frame = -1

Query: 955  VNGHEALSVYLEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIIMHIFISKGGESVMDA 776
            VNGHEALS Y+ +IEENV++ L++W+ MGE EFLT LRKLTFRIIM+IF+S   E+VMDA
Sbjct: 151  VNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIFLSSESENVMDA 210

Query: 775  LEEVYTTLNYGVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPAKKDM 596
            LE  YT LNYGVRAMA+N+PGFAY+RALK+RK LV  F S+V ERR  R ++  P KKDM
Sbjct: 211  LEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQREQNILPNKKDM 270

Query: 595  LDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKAKA-E 419
            LDNLL+VKDE+ R L+DEEIID L+MYLNAGHESSGH  MWAT+ LQEHP+  Q+AK  E
Sbjct: 271  LDNLLNVKDEDGRTLDDEEIIDVLLMYLNAGHESSGHTIMWATIFLQEHPEFLQRAKVNE 330

Query: 418  QEQIVKNRPSMQKGLRLKEIRQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYIIPM 239
            QE I+KNRP  QKGL LKE RQM++L++V+DETLR+I+FSL  FREAK DV MNGY+IP 
Sbjct: 331  QEMILKNRPEGQKGLTLKETRQMEFLSQVVDETLRVITFSLTAFREAKTDVEMNGYLIPK 390

Query: 238  GWKVEVWLRNVHRDPEVYPDPNNFDPSRWD-GFTPKAGTYLPFGAGSRLCPGNELAKLEI 62
            GWKV  W R+VH DPEVYPDP  FDPSRWD GF PKAG +LPFGAGS LCPGN+LAKLEI
Sbjct: 391  GWKVLTWFRDVHIDPEVYPDPRKFDPSRWDNGFVPKAGAFLPFGAGSHLCPGNDLAKLEI 450

Query: 61   AIFMHYFLLNYRVERLNPKC 2
            +IF+H+FLL Y+V+R NPKC
Sbjct: 451  SIFLHHFLLKYQVKRSNPKC 470


>ref|XP_002319447.1| cytochrome P450 probable ent-kaurenoic acid oxidase [Populus
            trichocarpa] gi|222857823|gb|EEE95370.1| cytochrome P450
            probable ent-kaurenoic acid oxidase [Populus trichocarpa]
          Length = 490

 Score =  462 bits (1190), Expect = e-128
 Identities = 220/319 (68%), Positives = 267/319 (83%), Gaps = 1/319 (0%)
 Frame = -1

Query: 955  VNGHEALSVYLEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIIMHIFISKGGESVMDA 776
            VNGHEALS Y+ +IE+NV++ LE+W+ MG+IEFLT++RKLTFRII++IF+SK  E VM+A
Sbjct: 149  VNGHEALSTYIPYIEQNVIAELEKWTTMGQIEFLTKMRKLTFRIIIYIFLSKTSERVMEA 208

Query: 775  LEEVYTTLNYGVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPAKK-D 599
            LE+ YTTLNYG+RAMAINLPGFAYY ALK+RK+LV IF S+V+ RR ++  D    KK D
Sbjct: 209  LEKEYTTLNYGIRAMAINLPGFAYYEALKARKKLVAIFQSIVDGRRNLKKDDVTNTKKKD 268

Query: 598  MLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKAKAE 419
            M+D+LLDV+DEN RKL DEE+ID ++MYLNAGHESSGHIT WAT+ LQ+HP+ FQKAK E
Sbjct: 269  MMDSLLDVEDENGRKLTDEEVIDIMLMYLNAGHESSGHITTWATIFLQDHPEYFQKAKEE 328

Query: 418  QEQIVKNRPSMQKGLRLKEIRQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYIIPM 239
            QEQI+K RP  QK L LKE+R+M YL+KVIDETLR+++FSL VFREAK D  MNGY IP 
Sbjct: 329  QEQIIKRRPLTQKRLSLKEVREMKYLSKVIDETLRMVTFSLTVFREAKTDFCMNGYTIPK 388

Query: 238  GWKVEVWLRNVHRDPEVYPDPNNFDPSRWDGFTPKAGTYLPFGAGSRLCPGNELAKLEIA 59
            GWKV  W R +H DPEVYP+P  F+PSRWD +TPKAGT+LPFGAGSRLCPGN LAKLEI+
Sbjct: 389  GWKVLAWFRTIHLDPEVYPNPKEFNPSRWDDYTPKAGTFLPFGAGSRLCPGNNLAKLEIS 448

Query: 58   IFMHYFLLNYRVERLNPKC 2
            IF+HYFLL+Y   R NP+C
Sbjct: 449  IFLHYFLLDY---RQNPEC 464