BLASTX nr result
ID: Coptis21_contig00026358
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00026358
(588 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFP55557.1| non-ltr retroelement reverse transcriptase [Rosa ... 45 3e-14
gb|AAD21778.1| putative non-LTR retroelement reverse transcripta... 44 2e-12
gb|EEE50691.1| hypothetical protein OsJ_30951 [Oryza sativa Japo... 48 4e-12
gb|ABE87589.2| RNA-directed DNA polymerase (Reverse transcriptas... 44 5e-12
pir||T00833 RNA-directed DNA polymerase homolog T13L16.7 - Arabi... 44 1e-11
>gb|AFP55557.1| non-ltr retroelement reverse transcriptase [Rosa rugosa]
Length = 1747
Score = 45.1 bits (105), Expect(3) = 3e-14
Identities = 22/43 (51%), Positives = 29/43 (67%)
Frame = -3
Query: 400 PENSIQLIANCVESPWFSEVLQGVYKGFFKSSGGLRQGDPLSP 272
P+ + LI +CV+S +S +LQG G K S GLRQGDP+SP
Sbjct: 887 PDRFVFLIMDCVKSVTYSVLLQGAPFGKIKPSRGLRQGDPISP 929
Score = 38.9 bits (89), Expect(3) = 3e-14
Identities = 19/40 (47%), Positives = 26/40 (65%), Gaps = 2/40 (5%)
Frame = -2
Query: 530 EFIQELPRKVRENN--IVFKFDMAKAYGRVNWGFLRRVLE 417
E I L R+ +++ I K DM KAY RV WGFL+R++E
Sbjct: 843 EIIHCLKRRGKDSRQKIALKLDMTKAYDRVEWGFLQRMME 882
Score = 38.9 bits (89), Expect(3) = 3e-14
Identities = 19/60 (31%), Positives = 30/60 (50%)
Frame = -1
Query: 183 RGCTGITHLLYADDLVVCLNGSKSLVDGLMQFMKEYEKCSSQLLNKNKSGCFVHRKAPTS 4
RG ++HL YADD ++ + + + L YE S Q +NK+KS K+P +
Sbjct: 959 RGAPSVSHLFYADDSLLFCDATVTDCMALKNIFSTYEAASGQKINKDKSAICFSPKSPAA 1018
>gb|AAD21778.1| putative non-LTR retroelement reverse transcriptase [Arabidopsis
thaliana]
Length = 1715
Score = 43.9 bits (102), Expect(4) = 2e-12
Identities = 21/61 (34%), Positives = 32/61 (52%)
Frame = -1
Query: 183 RGCTGITHLLYADDLVVCLNGSKSLVDGLMQFMKEYEKCSSQLLNKNKSGCFVHRKAPTS 4
+ C I+HLL+ADD + S ++ L K+YE+ S Q +N KS +K PT
Sbjct: 1033 KDCLAISHLLFADDSLFFCRASNQNIEQLALIFKKYEEASGQKINYAKSSIIFGQKIPTM 1092
Query: 3 K 1
+
Sbjct: 1093 R 1093
Score = 37.4 bits (85), Expect(4) = 2e-12
Identities = 24/61 (39%), Positives = 34/61 (55%)
Frame = -3
Query: 388 IQLIANCVESPWFSEVLQGVYKGFFKSSGGLRQGDPLSPVSVAYFK*EVLSHGLNEFQVR 209
++ I CV S + ++ G G S G+RQGDPLSP + F EVLS+ L + +V
Sbjct: 965 VKWIMTCVTSVSYEVLINGSPYGKIFPSRGIRQGDPLSPY-LFLFCAEVLSNMLRKAEVN 1023
Query: 208 K 206
K
Sbjct: 1024 K 1024
Score = 32.0 bits (71), Expect(4) = 2e-12
Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = -2
Query: 545 IFLTQEFIQELP--RKVRENNIVFKFDMAKAYGRVNWGFLRRVL 420
+ + E + L R+ + + K D++KAY RV W FL +V+
Sbjct: 912 VLVAHELLHSLKSRRECQSGYVAVKTDISKAYDRVEWNFLEKVM 955
Score = 22.7 bits (47), Expect(4) = 2e-12
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = -1
Query: 582 QGAFVKGSEISDNI 541
Q AFV G ISDN+
Sbjct: 899 QAAFVPGQNISDNV 912
>gb|EEE50691.1| hypothetical protein OsJ_30951 [Oryza sativa Japonica Group]
Length = 697
Score = 48.1 bits (113), Expect(3) = 4e-12
Identities = 19/49 (38%), Positives = 35/49 (71%)
Frame = -1
Query: 183 RGCTGITHLLYADDLVVCLNGSKSLVDGLMQFMKEYEKCSSQLLNKNKS 37
RG +THL +ADD ++ L ++S+ + + Q + +YE+CS Q++N++KS
Sbjct: 192 RGAPILTHLFFADDSLLLLKATESVANEMKQIILDYERCSGQIVNRDKS 240
Score = 36.2 bits (82), Expect(3) = 4e-12
Identities = 18/38 (47%), Positives = 22/38 (57%)
Frame = -3
Query: 385 QLIANCVESPWFSEVLQGVYKGFFKSSGGLRQGDPLSP 272
+LI NCV + + + G Y K GLRQGDPLSP
Sbjct: 125 RLIMNCVTTVNYKIKVNGDYTEVIKPDRGLRQGDPLSP 162
Score = 31.6 bits (70), Expect(3) = 4e-12
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = -2
Query: 554 LVIIFLTQEFIQELPRKVRENNIVFKFDMAKAYGRVNWGFLRRVL 420
++I + F+Q R ++ K DM+KAY RV W FL +L
Sbjct: 71 VLIAYEATHFLQN-KRNGKDGYAAIKLDMSKAYDRVEWPFLLHML 114
>gb|ABE87589.2| RNA-directed DNA polymerase (Reverse transcriptase); Ribonuclease
H; Endonuclease/exonuclease/phosphatase [Medicago
truncatula]
Length = 1246
Score = 43.9 bits (102), Expect(3) = 5e-12
Identities = 23/68 (33%), Positives = 38/68 (55%)
Frame = -1
Query: 231 VSMNFKSGKIGWFQTRRGCTGITHLLYADDLVVCLNGSKSLVDGLMQFMKEYEKCSSQLL 52
+SM G++ RG + TH+LYADD+++ G+K + L++ +Y + S QL+
Sbjct: 667 LSMAATDGQLIPMSYCRGVSFPTHILYADDVLIFCTGTKRNIRRLIKIFSQYSEVSGQLI 726
Query: 51 NKNKSGCF 28
N KS F
Sbjct: 727 NNAKSRFF 734
Score = 37.7 bits (86), Expect(3) = 5e-12
Identities = 23/58 (39%), Positives = 32/58 (55%)
Frame = -3
Query: 397 ENSIQLIANCVESPWFSEVLQGVYKGFFKSSGGLRQGDPLSPVSVAYFK*EVLSHGLN 224
E + I ++S S ++ G GFF S G+RQGDPLSP+ + EVLS L+
Sbjct: 612 EKFVHWILVILQSARLSVLVNGKAVGFFTCSHGVRQGDPLSPLLFCLVE-EVLSRALS 668
Score = 33.9 bits (76), Expect(3) = 5e-12
Identities = 15/43 (34%), Positives = 24/43 (55%)
Frame = -2
Query: 545 IFLTQEFIQELPRKVRENNIVFKFDMAKAYGRVNWGFLRRVLE 417
+ L E I L ++ N+ K D+AKA+ ++W FL VL+
Sbjct: 564 VILASEAINLLEKRQYGGNVALKVDIAKAFDTLDWNFLLAVLQ 606
>pir||T00833 RNA-directed DNA polymerase homolog T13L16.7 - Arabidopsis thaliana
(fragment)
Length = 1365
Score = 43.9 bits (102), Expect(3) = 1e-11
Identities = 21/60 (35%), Positives = 35/60 (58%)
Frame = -1
Query: 216 KSGKIGWFQTRRGCTGITHLLYADDLVVCLNGSKSLVDGLMQFMKEYEKCSSQLLNKNKS 37
++GKI Q + + HLL+ADD ++ +K + LMQ + +Y + S Q++N NKS
Sbjct: 659 QAGKITGIQFQDKKVSVNHLLFADDTLLMCKATKQECEELMQCLSQYGQLSGQMINLNKS 718
Score = 37.0 bits (84), Expect(3) = 1e-11
Identities = 23/56 (41%), Positives = 29/56 (51%)
Frame = -3
Query: 388 IQLIANCVESPWFSEVLQGVYKGFFKSSGGLRQGDPLSPVSVAYFK*EVLSHGLNE 221
I I NCV S +S ++ G G + G+RQGDPLSP E L H LN+
Sbjct: 602 ISWIMNCVTSVSYSVLINGQPYGHIIPTRGIRQGDPLSPALFVLCT-EALIHILNK 656
Score = 33.5 bits (75), Expect(3) = 1e-11
Identities = 16/44 (36%), Positives = 25/44 (56%), Gaps = 2/44 (4%)
Frame = -2
Query: 545 IFLTQEFIQELPR--KVRENNIVFKFDMAKAYGRVNWGFLRRVL 420
I + E I L ++ + ++ FK DM+KAY RV W FL ++
Sbjct: 549 ILVAHEMIHSLRTNDRISKEHMAFKTDMSKAYDRVEWPFLETMM 592