BLASTX nr result
ID: Coptis21_contig00026234
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00026234
(521 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_001190292.1| Alkaline phytoceramidase (aPHC) [Arabidopsis... 77 6e-38
ref|NP_196748.1| Alkaline phytoceramidase (aPHC) [Arabidopsis th... 77 6e-38
ref|XP_002516438.1| conserved hypothetical protein [Ricinus comm... 85 2e-37
ref|XP_003527749.1| PREDICTED: uncharacterized protein LOC100806... 83 1e-32
ref|XP_003523644.1| PREDICTED: uncharacterized protein LOC100796... 82 8e-32
>ref|NP_001190292.1| Alkaline phytoceramidase (aPHC) [Arabidopsis thaliana]
gi|332004350|gb|AED91733.1| Alkaline phytoceramidase
(aPHC) [Arabidopsis thaliana]
Length = 270
Score = 76.6 bits (187), Expect(3) = 6e-38
Identities = 28/61 (45%), Positives = 48/61 (78%)
Frame = +3
Query: 165 AFGSTYYHLKPEDNQVIWDRFPMMVAYVAFLSNLVLERVGMRIGITCLFSLLSLAVVSIA 344
AFGS +YHLKP+DN+++WD P+++AY + S+ ++ER G ++G++CL LL ++ +S+A
Sbjct: 106 AFGSAFYHLKPDDNRIVWDTLPILIAYSSLFSSFLVERAGEKVGLSCLILLLFISCLSVA 165
Query: 345 Y 347
Y
Sbjct: 166 Y 166
Score = 67.0 bits (162), Expect(3) = 6e-38
Identities = 29/44 (65%), Positives = 34/44 (77%)
Frame = +1
Query: 352 ACCRNFDYLRLCMMFHLIPSVVIPTLAIRYPPKYTHSRYWLWAT 483
A R F+ LRLCM F LIP +VIP +A+ PPKYTHSR+WLWAT
Sbjct: 165 AYARVFNDLRLCMTFQLIPCLVIPVMAVLLPPKYTHSRFWLWAT 208
Score = 59.7 bits (143), Expect(3) = 6e-38
Identities = 31/44 (70%), Positives = 34/44 (77%), Gaps = 1/44 (2%)
Frame = +2
Query: 56 GVPNTLNVITNFPFLIVGVFCLVLCLQGSFFAISWNC-IWVYLL 184
GVPNTLNV+TNFPFLIVGV VLC+ GSFF IS N IW + L
Sbjct: 54 GVPNTLNVMTNFPFLIVGVLGFVLCIGGSFFNISLNGEIWGWTL 97
>ref|NP_196748.1| Alkaline phytoceramidase (aPHC) [Arabidopsis thaliana]
gi|7573354|emb|CAB87660.1| putative protein [Arabidopsis
thaliana] gi|38566674|gb|AAR24227.1| At5g11870
[Arabidopsis thaliana] gi|40824251|gb|AAR92361.1|
At5g11870 [Arabidopsis thaliana]
gi|332004349|gb|AED91732.1| Alkaline phytoceramidase
(aPHC) [Arabidopsis thaliana]
Length = 262
Score = 76.6 bits (187), Expect(3) = 6e-38
Identities = 28/61 (45%), Positives = 48/61 (78%)
Frame = +3
Query: 165 AFGSTYYHLKPEDNQVIWDRFPMMVAYVAFLSNLVLERVGMRIGITCLFSLLSLAVVSIA 344
AFGS +YHLKP+DN+++WD P+++AY + S+ ++ER G ++G++CL LL ++ +S+A
Sbjct: 106 AFGSAFYHLKPDDNRIVWDTLPILIAYSSLFSSFLVERAGEKVGLSCLILLLFISCLSVA 165
Query: 345 Y 347
Y
Sbjct: 166 Y 166
Score = 67.0 bits (162), Expect(3) = 6e-38
Identities = 29/44 (65%), Positives = 34/44 (77%)
Frame = +1
Query: 352 ACCRNFDYLRLCMMFHLIPSVVIPTLAIRYPPKYTHSRYWLWAT 483
A R F+ LRLCM F LIP +VIP +A+ PPKYTHSR+WLWAT
Sbjct: 165 AYARVFNDLRLCMTFQLIPCLVIPVMAVLLPPKYTHSRFWLWAT 208
Score = 59.7 bits (143), Expect(3) = 6e-38
Identities = 31/44 (70%), Positives = 34/44 (77%), Gaps = 1/44 (2%)
Frame = +2
Query: 56 GVPNTLNVITNFPFLIVGVFCLVLCLQGSFFAISWNC-IWVYLL 184
GVPNTLNV+TNFPFLIVGV VLC+ GSFF IS N IW + L
Sbjct: 54 GVPNTLNVMTNFPFLIVGVLGFVLCIGGSFFNISLNGEIWGWTL 97
>ref|XP_002516438.1| conserved hypothetical protein [Ricinus communis]
gi|223544258|gb|EEF45779.1| conserved hypothetical
protein [Ricinus communis]
Length = 231
Score = 84.7 bits (208), Expect(3) = 2e-37
Identities = 35/64 (54%), Positives = 50/64 (78%)
Frame = +3
Query: 156 VGTAFGSTYYHLKPEDNQVIWDRFPMMVAYVAFLSNLVLERVGMRIGITCLFSLLSLAVV 335
+G AFGS YYHLKP+D++ +WD PMM+AY + S+ V+ERVG R+G++CLF LL + ++
Sbjct: 105 MGVAFGSAYYHLKPDDSRFMWDTLPMMIAYSSLFSSFVVERVGKRVGLSCLFGLLVVILL 164
Query: 336 SIAY 347
S AY
Sbjct: 165 STAY 168
Score = 64.7 bits (156), Expect(3) = 2e-37
Identities = 28/43 (65%), Positives = 31/43 (72%)
Frame = +1
Query: 352 ACCRNFDYLRLCMMFHLIPSVVIPTLAIRYPPKYTHSRYWLWA 480
A R F+ LRLCMMF LIP + IP + YPPKYTHS YWLWA
Sbjct: 167 AYARIFNDLRLCMMFQLIPCIAIPGMTFLYPPKYTHSIYWLWA 209
Score = 52.0 bits (123), Expect(3) = 2e-37
Identities = 27/46 (58%), Positives = 31/46 (67%), Gaps = 5/46 (10%)
Frame = +2
Query: 56 GVPNTLNVITNFPFLIVGVFCLVLCLQGSFFAIS-----WNCIWVY 178
GVPNTLNVIT FPFL+VGV VL ++GSFF IS W + Y
Sbjct: 56 GVPNTLNVITIFPFLVVGVVGFVLSIRGSFFNISLRGEVWGWVMFY 101
>ref|XP_003527749.1| PREDICTED: uncharacterized protein LOC100806703 [Glycine max]
Length = 277
Score = 82.8 bits (203), Expect(3) = 1e-32
Identities = 35/63 (55%), Positives = 49/63 (77%)
Frame = +3
Query: 159 GTAFGSTYYHLKPEDNQVIWDRFPMMVAYVAFLSNLVLERVGMRIGITCLFSLLSLAVVS 338
G AFGS YYHLKP+D++V+WD PMMVA+ + LS+LV+ER+G RIG+ C+F+L A +
Sbjct: 111 GVAFGSAYYHLKPDDHRVLWDTLPMMVAFSSLLSSLVVERLGQRIGLCCMFALNLAAFLC 170
Query: 339 IAY 347
+ Y
Sbjct: 171 VVY 173
Score = 54.7 bits (130), Expect(3) = 1e-32
Identities = 28/46 (60%), Positives = 33/46 (71%), Gaps = 1/46 (2%)
Frame = +2
Query: 50 SSGVPNTLNVITNFPFLIVGVFCLVLCLQGSFFAI-SWNCIWVYLL 184
S GVPNTLNV+TNFPFL+VGV LVL L+G F I S +W + L
Sbjct: 59 SPGVPNTLNVMTNFPFLVVGVLGLVLALEGGVFNISSQGEVWTWAL 104
Score = 47.8 bits (112), Expect(3) = 1e-32
Identities = 20/41 (48%), Positives = 27/41 (65%)
Frame = +1
Query: 361 RNFDYLRLCMMFHLIPSVVIPTLAIRYPPKYTHSRYWLWAT 483
R ++ +R CMMF L + IP +A+ Y KYTHSRYW +T
Sbjct: 175 RIYNDIRFCMMFQLTLPLAIPVIAVLYRSKYTHSRYWFIST 215
>ref|XP_003523644.1| PREDICTED: uncharacterized protein LOC100796199 [Glycine max]
Length = 275
Score = 82.4 bits (202), Expect(3) = 8e-32
Identities = 35/63 (55%), Positives = 49/63 (77%)
Frame = +3
Query: 159 GTAFGSTYYHLKPEDNQVIWDRFPMMVAYVAFLSNLVLERVGMRIGITCLFSLLSLAVVS 338
G AFGS YYHLKP+D++V+WD PMMVA+ + LS+LV+ER+G RIG+ C+F+L A +
Sbjct: 107 GVAFGSAYYHLKPDDHRVLWDTLPMMVAFSSLLSSLVVERLGQRIGLCCMFALNLAAFLC 166
Query: 339 IAY 347
+ Y
Sbjct: 167 VIY 169
Score = 51.2 bits (121), Expect(3) = 8e-32
Identities = 29/59 (49%), Positives = 37/59 (62%), Gaps = 1/59 (1%)
Frame = +2
Query: 11 KSPFSECFL*IPPSSGVPNTLNVITNFPFLIVGVFCLVLCLQGSFFAI-SWNCIWVYLL 184
+SP F+ + GVPNTLNV+TNFPFL+VGV LVL L+ F I S +W + L
Sbjct: 42 RSPKHHQFVDMRNLLGVPNTLNVMTNFPFLVVGVLGLVLALEEGVFNISSQGEVWTWAL 100
Score = 48.9 bits (115), Expect(3) = 8e-32
Identities = 20/41 (48%), Positives = 28/41 (68%)
Frame = +1
Query: 361 RNFDYLRLCMMFHLIPSVVIPTLAIRYPPKYTHSRYWLWAT 483
R ++ +R CMMF L + IP +A+ Y KYTHSRYW ++T
Sbjct: 171 RIYNDVRFCMMFQLTLPLAIPVIAVLYRSKYTHSRYWFFST 211