BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00024303
         (514 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AED99875.1| cytochrome P450 [Panax notoginseng]                    207   9e-52
ref|XP_003528553.1| PREDICTED: flavonoid 3'-monooxygenase-like [...   206   2e-51
ref|XP_003627305.1| Flavonoid 3',5'-hydroxylase [Medicago trunca...   205   4e-51
emb|CAA65580.1| cytochrome P450 [Nicotiana tabacum]                   205   4e-51
gb|AFK47419.1| unknown [Medicago truncatula]                          205   4e-51

>gb|AED99875.1| cytochrome P450 [Panax notoginseng]
          Length = 512

 Score =  207 bits (526), Expect = 9e-52
 Identities = 105/179 (58%), Positives = 138/179 (77%), Gaps = 9/179 (5%)
 Frame = -1

Query: 511 GNPITLRHHISSLTLNNITRMILGKKYLGES---------DELKKILDEWFLLNGVLNIG 359
           G  I L+ ++S+++LN I+RM+LGK+YL ES         +E KK+LDE FLLNGV NIG
Sbjct: 165 GQKIALKDYLSTVSLNVISRMVLGKRYLDESVDQKAVVRPEEFKKMLDELFLLNGVFNIG 224

Query: 358 DLIPWINFLDLQGYVKRMKALRNKIDHFLEHALEEHIAKRQDHLREFLPKDMLEVLLPLL 179
           D IPWI+FLDLQGYVKRMK +  K D FLEH L+EHIA+R+    +++ KDM+++LL  L
Sbjct: 225 DSIPWIDFLDLQGYVKRMKVVSKKFDRFLEHVLDEHIARRETEKGDYVAKDMVDLLLQ-L 283

Query: 178 ADNPKPELKLSRNQVKGLTLDLLAGGTETSATIIEWAMSELLKQPQIFEQATEELDRVI 2
           +D+P  E+KL R+ VK  T DLLAGGTE+SA  +EWA+S+LLK+P+IFE+ATEELDRVI
Sbjct: 284 SDDPTLEVKLERHGVKAFTQDLLAGGTESSAVTVEWAISQLLKKPEIFEKATEELDRVI 342


>ref|XP_003528553.1| PREDICTED: flavonoid 3'-monooxygenase-like [Glycine max]
          Length = 516

 Score =  206 bits (523), Expect = 2e-51
 Identities = 106/174 (60%), Positives = 138/174 (79%), Gaps = 7/174 (4%)
 Frame = -1

Query: 502 ITLRHHISSLTLNNITRMILGKKYLGES-------DELKKILDEWFLLNGVLNIGDLIPW 344
           I L+ H+SSL+LN I+RM+LGKKYL ES       DE KK+LDE FLLNGV NIGD IPW
Sbjct: 175 ILLKDHLSSLSLNVISRMVLGKKYLEESQNAVVSPDEFKKMLDELFLLNGVYNIGDFIPW 234

Query: 343 INFLDLQGYVKRMKALRNKIDHFLEHALEEHIAKRQDHLREFLPKDMLEVLLPLLADNPK 164
           I+FLDLQGY+KRMK L  K D F+EH L+EHI +R+  +++++ KDM++VLL  LA++P 
Sbjct: 235 IDFLDLQGYIKRMKTLSKKFDMFMEHVLDEHI-ERKKGIKDYVAKDMVDVLLQ-LAEDPT 292

Query: 163 PELKLSRNQVKGLTLDLLAGGTETSATIIEWAMSELLKQPQIFEQATEELDRVI 2
            E+KL R+ VK  T DL+AGGTE+SA  +EWA+SELL++P+IF++ATEELDRVI
Sbjct: 293 LEVKLERHGVKAFTQDLIAGGTESSAVTVEWAISELLRRPEIFKKATEELDRVI 346


>ref|XP_003627305.1| Flavonoid 3',5'-hydroxylase [Medicago truncatula]
           gi|84514145|gb|ABC59081.1| cytochrome P450 monooxygenase
           CYP92A29 [Medicago truncatula]
           gi|355521327|gb|AET01781.1| Flavonoid 3',5'-hydroxylase
           [Medicago truncatula]
          Length = 512

 Score =  205 bits (521), Expect = 4e-51
 Identities = 103/174 (59%), Positives = 140/174 (80%), Gaps = 7/174 (4%)
 Frame = -1

Query: 502 ITLRHHISSLTLNNITRMILGKKYLGESD-------ELKKILDEWFLLNGVLNIGDLIPW 344
           I ++ H+S+L+LN I+RM+LGKKYL ++D       E KK+LDE FLLNG+LNIGD IPW
Sbjct: 171 ILVKDHLSTLSLNVISRMVLGKKYLEKTDNAVISPDEFKKMLDELFLLNGILNIGDFIPW 230

Query: 343 INFLDLQGYVKRMKALRNKIDHFLEHALEEHIAKRQDHLREFLPKDMLEVLLPLLADNPK 164
           I+FLDLQGYVKRMK L  K D F+EH LEEHI +R+ ++++++ KDM++VLL  LA++P 
Sbjct: 231 IHFLDLQGYVKRMKTLSKKFDRFMEHVLEEHIERRK-NVKDYVAKDMVDVLLQ-LAEDPN 288

Query: 163 PELKLSRNQVKGLTLDLLAGGTETSATIIEWAMSELLKQPQIFEQATEELDRVI 2
            E+KL R+ VK  T DL+AGGTE+SA  +EWA+SEL+++P+IF++ATEELDRVI
Sbjct: 289 LEVKLERHGVKAFTQDLIAGGTESSAVTVEWAISELVRKPEIFKKATEELDRVI 342


>emb|CAA65580.1| cytochrome P450 [Nicotiana tabacum]
          Length = 498

 Score =  205 bits (521), Expect = 4e-51
 Identities = 107/177 (60%), Positives = 136/177 (76%), Gaps = 7/177 (3%)
 Frame = -1

Query: 511 GNPITLRHHISSLTLNNITRMILGKKYLGES-------DELKKILDEWFLLNGVLNIGDL 353
           G PI L+ ++ +L+LN I+RM+LGK+YL ES       +E KK+LDE FLLNGVLNIGD 
Sbjct: 165 GKPILLKDYLMTLSLNVISRMVLGKRYLDESKNSIVTPEEFKKMLDELFLLNGVLNIGDY 224

Query: 352 IPWINFLDLQGYVKRMKALRNKIDHFLEHALEEHIAKRQDHLREFLPKDMLEVLLPLLAD 173
           IPWI+F+DLQGYVKRMK L NK D FLE+ L+EH A+R      ++ KDM++VLL   AD
Sbjct: 225 IPWIDFMDLQGYVKRMKVLSNKFDRFLEYVLDEHNARRNAE-ENYVAKDMVDVLLQ-FAD 282

Query: 172 NPKPELKLSRNQVKGLTLDLLAGGTETSATIIEWAMSELLKQPQIFEQATEELDRVI 2
           +P  E+KL R+ VK  T DLLAGGTE++A I+EWA+SELLK+P+IF +ATEELDRVI
Sbjct: 283 DPTLEVKLERHGVKAFTQDLLAGGTESTAVIVEWAISELLKKPEIFNKATEELDRVI 339


>gb|AFK47419.1| unknown [Medicago truncatula]
          Length = 512

 Score =  205 bits (521), Expect = 4e-51
 Identities = 103/174 (59%), Positives = 140/174 (80%), Gaps = 7/174 (4%)
 Frame = -1

Query: 502 ITLRHHISSLTLNNITRMILGKKYLGESD-------ELKKILDEWFLLNGVLNIGDLIPW 344
           I ++ H+S+L+LN I+RM+LGKKYL ++D       E KK+LDE FLLNG+LNIGD IPW
Sbjct: 171 ILVKDHLSTLSLNVISRMVLGKKYLEKTDNAVISPDEFKKMLDELFLLNGILNIGDFIPW 230

Query: 343 INFLDLQGYVKRMKALRNKIDHFLEHALEEHIAKRQDHLREFLPKDMLEVLLPLLADNPK 164
           I+FLDLQGYVKRMK L  K D F+EH LEEHI +R+ ++++++ KDM++VLL  LA++P 
Sbjct: 231 IHFLDLQGYVKRMKTLSKKFDRFMEHVLEEHIERRK-NVKDYVAKDMVDVLLQ-LAEDPN 288

Query: 163 PELKLSRNQVKGLTLDLLAGGTETSATIIEWAMSELLKQPQIFEQATEELDRVI 2
            E+KL R+ VK  T DL+AGGTE+SA  +EWA+SEL+++P+IF++ATEELDRVI
Sbjct: 289 LEVKLERHGVKAFTQDLIAGGTESSAVTVEWAVSELVRKPEIFKKATEELDRVI 342