BLASTX nr result
ID: Coptis21_contig00023843
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00023843
(1421 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI23243.3| unnamed protein product [Vitis vinifera] 558 e-156
ref|XP_002268884.1| PREDICTED: tRNA wybutosine-synthesizing prot... 558 e-156
ref|XP_004155603.1| PREDICTED: tRNA wybutosine-synthesizing prot... 541 e-151
ref|XP_004134760.1| PREDICTED: LOW QUALITY PROTEIN: tRNA wybutos... 541 e-151
ref|XP_003610434.1| tRNA wybutosine-synthesizing protein 2/3/4 [... 526 e-147
>emb|CBI23243.3| unnamed protein product [Vitis vinifera]
Length = 1013
Score = 558 bits (1438), Expect = e-156
Identities = 291/476 (61%), Positives = 351/476 (73%), Gaps = 7/476 (1%)
Frame = +2
Query: 5 MEFEKRKAATLTSISSTELDKSPKGTIDTPIIPLLKTINNHPSYFTTSSCSGRISILLQP 184
MEFEKRKAATL S+ STE DKSPKGTID I+PLL IN H SYFTTSSCSGRISIL QP
Sbjct: 1 MEFEKRKAATLASMGSTEPDKSPKGTIDRHIVPLLSAINRHNSYFTTSSCSGRISILSQP 60
Query: 185 LPQXXXXXXXXXXXXXRWLFITHELANANIVIDLLFPPNK-STEDGELVFRFEPFILAVE 361
P WLFITH+LA+ N V+ LLFP S++ +LVFRFEPFI+AVE
Sbjct: 61 SPAATNAHKKARGGS--WLFITHDLADPNSVLALLFPTTGCSSQHDDLVFRFEPFIVAVE 118
Query: 362 CKDVSAAQSLVATAISCGFRESGITSVNNKRVIIAIRCSIRLEVPLGTVGNVMVSKEYAE 541
CKDV+AAQ LV+TA+SCGFRESGITSV+ KRV++A+RCSIRLEVPLG G V+VS EY
Sbjct: 119 CKDVAAAQLLVSTAVSCGFRESGITSVS-KRVMVAVRCSIRLEVPLGGGGRVLVSPEYVR 177
Query: 542 YLVGIANEKMESNRKRTEGFLQVLESE-----MMGREEKDGCCG-EENGNVDSRQMAVGE 703
YLVGIAN+KME+NR+RTEGFLQ L+S G DG G +E+G D + G+
Sbjct: 178 YLVGIANDKMETNRRRTEGFLQALQSSGFVESFNGGAGLDGAMGGDEHGCSDCKD---GD 234
Query: 704 CDLGREDSNAQSGLLNAHWGSISAVKISTIGEPVDKLFLWGHSACTFYNSLQQKVLIFGG 883
+ R + +SG + S+S V++ I EPV+KLFLWGHSACT +KV++FGG
Sbjct: 235 ANSERIIAEKESGSVGVLGCSLSIVQMEIIDEPVEKLFLWGHSACTLDTINHKKVIVFGG 294
Query: 884 FGGLGRHARRNDTLTLDLQSGALKVISVEGPPSPRLGHTSSVVGELVFVIGGRGDPTKIL 1063
FGG+GRHARRND LD G LK ++ EG PSPRLGHTSS+VG+L+F+IGGR DP IL
Sbjct: 295 FGGMGRHARRNDAFVLDPLHGTLKAVNAEGTPSPRLGHTSSMVGDLMFIIGGRADPENIL 354
Query: 1064 NDVWVFNTIKNQWKLLECSGSVFLPRHRHAAAVVGSRIYVFGGLNNEIIYSSLYVLDSEN 1243
++VWV +T KN+W+ LEC+GSVF PRHRHAAAV+GS+IYVFGGLNN+ I SSL+VLD++N
Sbjct: 355 DNVWVLDTAKNEWRRLECTGSVFPPRHRHAAAVLGSKIYVFGGLNNDAISSSLHVLDTDN 414
Query: 1244 SQWSEITTKGEQPCSRHSHALVANDSQLFMFGGYDGEKVFSDLYRFDTIACLWKKE 1411
QW+EI GE PC+RHSH+LVA S+LFMFGG + K DLY FD CLWKKE
Sbjct: 415 LQWNEIRVHGEWPCARHSHSLVAYGSKLFMFGGCNDGKALGDLYSFDVQTCLWKKE 470
>ref|XP_002268884.1| PREDICTED: tRNA wybutosine-synthesizing protein 2/3/4-like [Vitis
vinifera]
Length = 1018
Score = 558 bits (1438), Expect = e-156
Identities = 291/476 (61%), Positives = 351/476 (73%), Gaps = 7/476 (1%)
Frame = +2
Query: 5 MEFEKRKAATLTSISSTELDKSPKGTIDTPIIPLLKTINNHPSYFTTSSCSGRISILLQP 184
MEFEKRKAATL S+ STE DKSPKGTID I+PLL IN H SYFTTSSCSGRISIL QP
Sbjct: 1 MEFEKRKAATLASMGSTEPDKSPKGTIDRHIVPLLSAINRHNSYFTTSSCSGRISILSQP 60
Query: 185 LPQXXXXXXXXXXXXXRWLFITHELANANIVIDLLFPPNK-STEDGELVFRFEPFILAVE 361
P WLFITH+LA+ N V+ LLFP S++ +LVFRFEPFI+AVE
Sbjct: 61 SPAATNAHKKARGGS--WLFITHDLADPNSVLALLFPTTGCSSQHDDLVFRFEPFIVAVE 118
Query: 362 CKDVSAAQSLVATAISCGFRESGITSVNNKRVIIAIRCSIRLEVPLGTVGNVMVSKEYAE 541
CKDV+AAQ LV+TA+SCGFRESGITSV+ KRV++A+RCSIRLEVPLG G V+VS EY
Sbjct: 119 CKDVAAAQLLVSTAVSCGFRESGITSVS-KRVMVAVRCSIRLEVPLGGGGRVLVSPEYVR 177
Query: 542 YLVGIANEKMESNRKRTEGFLQVLESE-----MMGREEKDGCCG-EENGNVDSRQMAVGE 703
YLVGIAN+KME+NR+RTEGFLQ L+S G DG G +E+G D + G+
Sbjct: 178 YLVGIANDKMETNRRRTEGFLQALQSSGFVESFNGGAGLDGAMGGDEHGCSDCKD---GD 234
Query: 704 CDLGREDSNAQSGLLNAHWGSISAVKISTIGEPVDKLFLWGHSACTFYNSLQQKVLIFGG 883
+ R + +SG + S+S V++ I EPV+KLFLWGHSACT +KV++FGG
Sbjct: 235 ANSERIIAEKESGSVGVLGCSLSIVQMEIIDEPVEKLFLWGHSACTLDTINHKKVIVFGG 294
Query: 884 FGGLGRHARRNDTLTLDLQSGALKVISVEGPPSPRLGHTSSVVGELVFVIGGRGDPTKIL 1063
FGG+GRHARRND LD G LK ++ EG PSPRLGHTSS+VG+L+F+IGGR DP IL
Sbjct: 295 FGGMGRHARRNDAFVLDPLHGTLKAVNAEGTPSPRLGHTSSMVGDLMFIIGGRADPENIL 354
Query: 1064 NDVWVFNTIKNQWKLLECSGSVFLPRHRHAAAVVGSRIYVFGGLNNEIIYSSLYVLDSEN 1243
++VWV +T KN+W+ LEC+GSVF PRHRHAAAV+GS+IYVFGGLNN+ I SSL+VLD++N
Sbjct: 355 DNVWVLDTAKNEWRRLECTGSVFPPRHRHAAAVLGSKIYVFGGLNNDAISSSLHVLDTDN 414
Query: 1244 SQWSEITTKGEQPCSRHSHALVANDSQLFMFGGYDGEKVFSDLYRFDTIACLWKKE 1411
QW+EI GE PC+RHSH+LVA S+LFMFGG + K DLY FD CLWKKE
Sbjct: 415 LQWNEIRVHGEWPCARHSHSLVAYGSKLFMFGGCNDGKALGDLYSFDVQTCLWKKE 470
>ref|XP_004155603.1| PREDICTED: tRNA wybutosine-synthesizing protein 2/3/4-like [Cucumis
sativus]
Length = 1035
Score = 541 bits (1393), Expect = e-151
Identities = 282/484 (58%), Positives = 348/484 (71%), Gaps = 12/484 (2%)
Frame = +2
Query: 5 MEFEKRKAATLTSISSTELDKSPKGTIDTPIIPLLKTINNHPSYFTTSSCSGRISILLQP 184
MEFEKRKAAT+ S+ STE DKSPKG++DTPIIPL+ T+N+HPSYFTTSSCSGRISIL QP
Sbjct: 1 MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNSHPSYFTTSSCSGRISILAQP 60
Query: 185 LP--QXXXXXXXXXXXXXRWLFITHELANANIVIDLLF-PPNKSTEDGELVFRFEPFILA 355
+ WLF++HE A N VIDLLF P+ + E ELVFRFEP I+A
Sbjct: 61 ISTTSAAAPKPKKKALGGSWLFVSHEFAEPNSVIDLLFRSPSTNRELSELVFRFEPLIIA 120
Query: 356 VECKDVSAAQSLVATAISCGFRESGITSVNNKRVIIAIRCSIRLEVPLGTVGNVMVSKEY 535
VECKD+ +AQ+LV+TAISCGFRESGITS + KRVIIAIRCSIR+EVPLGT +MV+ EY
Sbjct: 121 VECKDLGSAQALVSTAISCGFRESGITSAS-KRVIIAIRCSIRMEVPLGTSEKIMVTPEY 179
Query: 536 AEYLVGIANEKMESNRKRTEGFLQVLESEMMGREEKDGCCG----EENGNV-DSRQMAVG 700
+YLV +ANEKM +N+KRT+GFL+ L+S + C G E NV DS
Sbjct: 180 VQYLVNVANEKMVANKKRTDGFLKGLQSSISDASRT--CHGIPSREATENVNDSLDSKGH 237
Query: 701 ECDLGREDSNAQSGLLNAHWG----SISAVKISTIGEPVDKLFLWGHSACTFYNSLQQKV 868
+C G + + + G+ NA+ S+S +I GEP++KLF+WGHSA T ++ KV
Sbjct: 238 DCADGDDGAALEGGVRNANSEACSYSLSVEQIDIAGEPIEKLFIWGHSATTIHD----KV 293
Query: 869 LIFGGFGGLGRHARRNDTLTLDLQSGALKVISVEGPPSPRLGHTSSVVGELVFVIGGRGD 1048
+ FGGFGG+GRHARRND L LD+ S L+ I+VE PSPRLGHTSS+VG+ ++V+GGR D
Sbjct: 294 IAFGGFGGMGRHARRNDLLLLDMLSYTLQTINVEDSPSPRLGHTSSLVGDRLYVVGGRTD 353
Query: 1049 PTKILNDVWVFNTIKNQWKLLECSGSVFLPRHRHAAAVVGSRIYVFGGLNNEIIYSSLYV 1228
PT ILNDVW+FN + +W LLEC+GS F PRHRHAAA +GS+IYVFGGL N+ I SS
Sbjct: 354 PTCILNDVWLFNITQEKWTLLECTGSPFSPRHRHAAAALGSKIYVFGGLENDRISSSFIF 413
Query: 1229 LDSENSQWSEITTKGEQPCSRHSHALVANDSQLFMFGGYDGEKVFSDLYRFDTIACLWKK 1408
LDS++ QW EI GEQPC RHSH++V+ S ++MFGGYDGEK DLY FDT AC WKK
Sbjct: 414 LDSDSHQWKEIQAGGEQPCGRHSHSMVSYGSHIYMFGGYDGEKTLGDLYSFDTNACYWKK 473
Query: 1409 EKTA 1420
E A
Sbjct: 474 ENIA 477
>ref|XP_004134760.1| PREDICTED: LOW QUALITY PROTEIN: tRNA wybutosine-synthesizing protein
2/3/4-like [Cucumis sativus]
Length = 1034
Score = 541 bits (1393), Expect = e-151
Identities = 282/484 (58%), Positives = 348/484 (71%), Gaps = 12/484 (2%)
Frame = +2
Query: 5 MEFEKRKAATLTSISSTELDKSPKGTIDTPIIPLLKTINNHPSYFTTSSCSGRISILLQP 184
MEFEKRKAAT+ S+ STE DKSPKG++DTPIIPL+ T+N+HPSYFTTSSCSGRISIL QP
Sbjct: 1 MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNSHPSYFTTSSCSGRISILAQP 60
Query: 185 LP--QXXXXXXXXXXXXXRWLFITHELANANIVIDLLF-PPNKSTEDGELVFRFEPFILA 355
+ WLF++HE A N VIDLLF P+ + E ELVFRFEP I+A
Sbjct: 61 ISTTSAAAPKPKKKALGGSWLFVSHEFAEPNSVIDLLFRSPSTNRELSELVFRFEPLIIA 120
Query: 356 VECKDVSAAQSLVATAISCGFRESGITSVNNKRVIIAIRCSIRLEVPLGTVGNVMVSKEY 535
VECKD+ +AQ+LV+TAISCGFRESGITS + KRVIIAIRCSIR+EVPLGT +MV+ EY
Sbjct: 121 VECKDLGSAQALVSTAISCGFRESGITSAS-KRVIIAIRCSIRMEVPLGTSEKIMVTPEY 179
Query: 536 AEYLVGIANEKMESNRKRTEGFLQVLESEMMGREEKDGCCG----EENGNV-DSRQMAVG 700
+YLV +ANEKM +N+KRT+GFL+ L+S + C G E NV DS
Sbjct: 180 VQYLVNVANEKMVANKKRTDGFLKGLQSSISDASRT--CHGIPSREATENVNDSLDSKGH 237
Query: 701 ECDLGREDSNAQSGLLNAHWG----SISAVKISTIGEPVDKLFLWGHSACTFYNSLQQKV 868
+C G + + + G+ NA+ S+S +I GEP++KLF+WGHSA T ++ KV
Sbjct: 238 DCADGDDGAALEGGVRNANSEACSYSLSVEQIDIAGEPIEKLFIWGHSATTIHD----KV 293
Query: 869 LIFGGFGGLGRHARRNDTLTLDLQSGALKVISVEGPPSPRLGHTSSVVGELVFVIGGRGD 1048
+ FGGFGG+GRHARRND L LD+ S L+ I+VE PSPRLGHTSS+VG+ ++V+GGR D
Sbjct: 294 IAFGGFGGMGRHARRNDLLLLDMLSYTLQTINVEDSPSPRLGHTSSLVGDRLYVVGGRTD 353
Query: 1049 PTKILNDVWVFNTIKNQWKLLECSGSVFLPRHRHAAAVVGSRIYVFGGLNNEIIYSSLYV 1228
PT ILNDVW+FN + +W LLEC+GS F PRHRHAAA +GS+IYVFGGL N+ I SS
Sbjct: 354 PTCILNDVWLFNITQEKWTLLECTGSPFSPRHRHAAAALGSKIYVFGGLENDRISSSFIF 413
Query: 1229 LDSENSQWSEITTKGEQPCSRHSHALVANDSQLFMFGGYDGEKVFSDLYRFDTIACLWKK 1408
LDS++ QW EI GEQPC RHSH++V+ S ++MFGGYDGEK DLY FDT AC WKK
Sbjct: 414 LDSDSHQWKEIQAGGEQPCGRHSHSMVSYGSHIYMFGGYDGEKTLGDLYSFDTNACYWKK 473
Query: 1409 EKTA 1420
E A
Sbjct: 474 ENIA 477
>ref|XP_003610434.1| tRNA wybutosine-synthesizing protein 2/3/4 [Medicago truncatula]
gi|355511489|gb|AES92631.1| tRNA wybutosine-synthesizing
protein 2/3/4 [Medicago truncatula]
Length = 1046
Score = 526 bits (1354), Expect = e-147
Identities = 278/489 (56%), Positives = 346/489 (70%), Gaps = 17/489 (3%)
Frame = +2
Query: 5 MEFEKRKAATLTSISSTELDKSPKGTIDTPIIPLLKTINNHPSYFTTSSCSGRISILLQP 184
MEFEKRK+ATL S++STE DKSPKG++DTPIIPL+ T+N +P+YFTTSSCSGRISIL QP
Sbjct: 1 MEFEKRKSATLASLNSTESDKSPKGSLDTPIIPLINTLNQNPNYFTTSSCSGRISILSQP 60
Query: 185 LPQXXXXXXXXXXXXXRWLFITHELANANIVIDLLFPPNK--STED--GELVFRFEPFIL 352
L WLF++H AN + +I LLFP + ST+ ELVFRFEP I+
Sbjct: 61 LSPIPSPQTKKKAKGGTWLFVSHHTANPDSIISLLFPSDSTHSTQSPISELVFRFEPLII 120
Query: 353 AVECKDVSAAQSLVATAISCGFRESGITSVNNKRVIIAIRCSIRLEVPLGTVGNVMVSKE 532
A+ECK++S+AQSLVA AIS GFRESGIT+ N KRVIIAIRCSIR+EVPLG +MV+ E
Sbjct: 121 AIECKELSSAQSLVALAISSGFRESGITNAN-KRVIIAIRCSIRMEVPLGDTQKIMVTPE 179
Query: 533 YAEYLVGIANEKMESNRKRTEGFLQVLESEMMGREEKDGCCGEENGNVDSRQMAVG-ECD 709
Y +YLV +ANEKME+N RT+ FL++L++ +G +N N S++ V CD
Sbjct: 180 YVKYLVQVANEKMEANWNRTDRFLRLLQN--------NGSMVNDNSNRLSQRTGVELVCD 231
Query: 710 LGREDSNAQ----------SGLLNAHWGSISAVKISTIGEPVDKLFLWGHSACTFYNSLQ 859
+ D +Q SG + + S+ I +GEPV+KLFLWGHSAC N+
Sbjct: 232 NLQFDHESQITNGNAPKNESGFVGSPGFSLPIAHIEVVGEPVEKLFLWGHSACALDNADH 291
Query: 860 QKVLIFGGFGGLGRHARRNDTLTLDLQSGALKVISVEGP--PSPRLGHTSSVVGELVFVI 1033
+KV++FGGFGGLGRHARRND L LD S L+ I G PSPRLGHT+S+VG+L+FVI
Sbjct: 292 KKVIVFGGFGGLGRHARRNDLLLLDPYSFNLETIDTSGCACPSPRLGHTASLVGDLMFVI 351
Query: 1034 GGRGDPTKILNDVWVFNTIKNQWKLLECSGSVFLPRHRHAAAVVGSRIYVFGGLNNEIIY 1213
GGR P KILNDVW F+T KN WKLL+C GSVF PRHRHAAAV+GS IYVFGGL+N+II+
Sbjct: 352 GGRTGPDKILNDVWSFDTTKNCWKLLQCGGSVFPPRHRHAAAVMGSNIYVFGGLDNDIIF 411
Query: 1214 SSLYVLDSENSQWSEITTKGEQPCSRHSHALVANDSQLFMFGGYDGEKVFSDLYRFDTIA 1393
SS ++LD+ N W EI G+ P +RHSHA+VA+DS++FMFGGYDG K D+Y FD
Sbjct: 412 SSFFILDTVNLHWKEIPVSGDWPYARHSHAMVASDSRIFMFGGYDGGKALGDMYSFDVQM 471
Query: 1394 CLWKKEKTA 1420
WKKE TA
Sbjct: 472 SQWKKEITA 480