BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00023718
         (1292 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]       415   e-113
gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago ...   379   e-105
dbj|BAL45198.1| cytochrome P450 monooxygenase [Medicago truncatula]   379   e-105
ref|XP_002265455.1| PREDICTED: secologanin synthase isoform 1 [V...   375   e-102
emb|CBI39916.3| unnamed protein product [Vitis vinifera]              375   e-102

>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 518

 Score =  415 bits (1067), Expect = e-113
 Identities = 198/352 (56%), Positives = 270/352 (76%)
 Frame = +1

Query: 229  CSLFYFGDFMGIESFLSDMVEAQKD*KYLRKQGIKGSPYKLLVGNFLELAKVTNEAQSHP 408
            C++ Y   +   ++F+   +  +K  K LR+QGIKG  YK L GN  ++   T EA++ P
Sbjct: 13   CAVIYISSWAW-KAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDIVSATQEARAKP 71

Query: 409  MELSHKIVPRVVPFIYRTIEKYGKTTLAWYGPTPRVVILDPEVTKDIMTSQFRDFPKPKS 588
            MELSHKIVPRV+PF+++T+EKYGK +  WYGP  RV I+DP++ +DI++++F  F K ++
Sbjct: 72   MELSHKIVPRVMPFVHQTVEKYGKLSFIWYGPYCRVNIMDPDMIRDILSNKFGHFEKIRT 131

Query: 589  NSFVDFVLTGLVTYEGEQWTKHRKILNPAFHLEKLKLMLPAFHTTCSELISKWEKQVSEK 768
            N     +++G+V+YEGE+W KHR+ILNPAFHLEKLK+MLPA +T+CSE+++KWE  VS+ 
Sbjct: 132  NPLGKLLISGVVSYEGEKWVKHRRILNPAFHLEKLKMMLPAIYTSCSEMVNKWEMMVSQD 191

Query: 769  SCEVELCHDFDKLTGDVISRTAFGSNFEQGRRIFELQVEQAHLVVKISPYIFIPGLRFLP 948
            SCE+++      LT DVISRTAFGS++E+GRRIF+LQ EQA LV+K    I+IPGLRFLP
Sbjct: 192  SCELDVWPHLQNLTADVISRTAFGSSYEEGRRIFQLQSEQAGLVIKALQSIYIPGLRFLP 251

Query: 949  ITINKRLKETGREIKTILNDIINQREKAMNFTELHHNDVLGLLMESNLKEMKEHGNSKNA 1128
               N R+KE   E + +L DI+N+REKAM   E H++D+LGLLMESN KE+ ++ NSKN 
Sbjct: 252  TKRNNRMKEIYGEARVLLRDIVNKREKAMKIGEAHNDDLLGLLMESNFKEITDNVNSKNF 311

Query: 1129 GITLEEVIDECKLFYLAGQETVSTLMVWTMVLLSMHQEWQVRAREEVLKLFG 1284
            G+T++EVIDECKLFY AGQET STL+VWTMV+LSMH +WQ +AREEVL++FG
Sbjct: 312  GMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFG 363


>gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago truncatula]
          Length = 518

 Score =  379 bits (974), Expect(2) = e-105
 Identities = 182/327 (55%), Positives = 244/327 (74%), Gaps = 1/327 (0%)
 Frame = +1

Query: 307  KYLRKQGIKGSPYKLLVGNFLELAKVTNEAQSHPMELSHKIVPRVVPFIYRTIEKYGKTT 486
            K LR+QG+KG+ Y+LLVG+  +L K+  EA S PM LS  IVPRV  F+++++ K+GK +
Sbjct: 36   KLLREQGLKGNSYRLLVGDMNDLLKMRKEATSKPMNLSDDIVPRVYSFVHQSVTKHGKNS 95

Query: 487  LAWYGPTPRVVILDPEVTKDIMTSQFRDFPKPKSNSFVDFVLTGLVTYEGEQWTKHRKIL 666
              W+GPTPRV I DP+  KD++   + DF KP +N  V  +  GLV+ EGEQW+KHRKI+
Sbjct: 96   FIWFGPTPRVNITDPDQIKDVLNKIY-DFQKPNANPLVRLLANGLVSLEGEQWSKHRKII 154

Query: 667  NPAFHLEKLKLMLPAFHTTCSELISKWEKQVS-EKSCEVELCHDFDKLTGDVISRTAFGS 843
            NPAFHLEKLK+MLP FH +C +LISKWEK +S + SCE+++         DVISR AFGS
Sbjct: 155  NPAFHLEKLKIMLPIFHKSCDDLISKWEKMLSSDGSCEMDVWPFLQNFASDVISRAAFGS 214

Query: 844  NFEQGRRIFELQVEQAHLVVKISPYIFIPGLRFLPITINKRLKETGREIKTILNDIINQR 1023
            ++E+G+RIF+LQ EQA L  KI   ++IPG RFLP    +R+KE  R+IK  L D+IN R
Sbjct: 215  SYEEGKRIFQLQTEQAELTTKIMMKVYIPGWRFLPTATPRRMKEIDRDIKASLTDMINNR 274

Query: 1024 EKAMNFTELHHNDVLGLLMESNLKEMKEHGNSKNAGITLEEVIDECKLFYLAGQETVSTL 1203
            E+A+   E   +D+LG+L+ESN KEM+EHGN+K+ G++L++VI+ECKLFY AGQET S L
Sbjct: 275  ERALKAGEATKDDLLGILLESNHKEMEEHGNNKDVGMSLDDVIEECKLFYFAGQETTSVL 334

Query: 1204 MVWTMVLLSMHQEWQVRAREEVLKLFG 1284
            +VWTMVLLS + +WQ RAREEVL++FG
Sbjct: 335  LVWTMVLLSRYPDWQARAREEVLQVFG 361



 Score = 29.3 bits (64), Expect(2) = e-105
 Identities = 10/22 (45%), Positives = 16/22 (72%)
 Frame = +2

Query: 242 TLVISWALKVFYLIWLKPKRIE 307
           TL + WA ++   +WLKPK++E
Sbjct: 14  TLSLIWAWRILNWLWLKPKKLE 35


>dbj|BAL45198.1| cytochrome P450 monooxygenase [Medicago truncatula]
          Length = 518

 Score =  379 bits (972), Expect(2) = e-105
 Identities = 180/327 (55%), Positives = 244/327 (74%), Gaps = 1/327 (0%)
 Frame = +1

Query: 307  KYLRKQGIKGSPYKLLVGNFLELAKVTNEAQSHPMELSHKIVPRVVPFIYRTIEKYGKTT 486
            K LR+QG+KG+ Y+LLVG+  +L K+  EA + PM LS  IVPRV  F+++++ K+GK +
Sbjct: 36   KLLREQGLKGNSYRLLVGDIKDLLKIRKEATTKPMNLSDDIVPRVYSFVHQSVTKHGKNS 95

Query: 487  LAWYGPTPRVVILDPEVTKDIMTSQFRDFPKPKSNSFVDFVLTGLVTYEGEQWTKHRKIL 666
              W+GPTPRV I DP+  KD++   + DF KP +N  V  +  GLV+ EGEQW+KHRKI+
Sbjct: 96   FIWFGPTPRVNITDPDQIKDVLNKIY-DFQKPNANPLVRLLANGLVSLEGEQWSKHRKII 154

Query: 667  NPAFHLEKLKLMLPAFHTTCSELISKWEKQVS-EKSCEVELCHDFDKLTGDVISRTAFGS 843
            NPAFHLEKLK+MLP FH +C ++ISKWEK +S + SCE+++         DVISR AFGS
Sbjct: 155  NPAFHLEKLKIMLPIFHKSCDDMISKWEKMLSSDGSCEMDVWPFLQNFASDVISRAAFGS 214

Query: 844  NFEQGRRIFELQVEQAHLVVKISPYIFIPGLRFLPITINKRLKETGREIKTILNDIINQR 1023
            ++E+G+RIF+LQ EQA L  KI   ++IPG RFLP    +R+KE  R+IK  L D+IN R
Sbjct: 215  SYEEGKRIFQLQTEQAELTTKIMMKVYIPGWRFLPTATPRRMKEIDRDIKASLTDMINNR 274

Query: 1024 EKAMNFTELHHNDVLGLLMESNLKEMKEHGNSKNAGITLEEVIDECKLFYLAGQETVSTL 1203
            E+A+   E   +D+LG+L+ESN KEM+EHGN+K+ G++L++VI+ECKLFY AGQET S L
Sbjct: 275  ERALKAGEATKDDLLGILLESNHKEMEEHGNNKDVGMSLDDVIEECKLFYFAGQETTSVL 334

Query: 1204 MVWTMVLLSMHQEWQVRAREEVLKLFG 1284
            +VWTMVLLS + +WQ RAREEVL++FG
Sbjct: 335  LVWTMVLLSRYPDWQARAREEVLQVFG 361



 Score = 30.0 bits (66), Expect(2) = e-105
 Identities = 10/28 (35%), Positives = 19/28 (67%)
 Frame = +2

Query: 224 LVVVCSTLVISWALKVFYLIWLKPKRIE 307
           +++   TL + WA ++   +WLKPK++E
Sbjct: 8   ILLTLMTLSLIWAWRILNWLWLKPKKLE 35


>ref|XP_002265455.1| PREDICTED: secologanin synthase isoform 1 [Vitis vinifera]
          Length = 515

 Score =  375 bits (963), Expect(2) = e-102
 Identities = 179/329 (54%), Positives = 245/329 (74%), Gaps = 1/329 (0%)
 Frame = +1

Query: 307  KYLRKQGIKGSPYKLLVGNFLELAKVTNEAQSHPMELSHKIVPRVVPFIYRTIEKYGKTT 486
            +YLR+QG+ G+ Y+LL G+F E++++ +EA S P+ LS  IV RV+PF Y +I+KYGK  
Sbjct: 35   RYLRQQGLIGNSYRLLHGDFREMSRMIDEANSRPISLSDDIVQRVLPFHYHSIKKYGKNC 94

Query: 487  LAWYGPTPRVVILDPEVTKDIMTSQFRDFPKPKSNSFVDFVLTGLVTYEGEQWTKHRKIL 666
              W GP P V I++PE+ +D++      F KP  +     + TG++  EGEQWTK RKI+
Sbjct: 95   FIWMGPKPVVNIMEPELIRDVLLKH-NAFQKPPVHPLGKLLATGVIALEGEQWTKRRKII 153

Query: 667  NPAFHLEKLKLMLPAFHTTCSELISKWEKQVSEK-SCEVELCHDFDKLTGDVISRTAFGS 843
            NPAFHLEKLK M+PAF  +CSE+++KWEK++S+  SCE+++  D + L GDVISRTAFGS
Sbjct: 154  NPAFHLEKLKHMVPAFQLSCSEMVNKWEKKLSKDGSCELDIWPDLENLAGDVISRTAFGS 213

Query: 844  NFEQGRRIFELQVEQAHLVVKISPYIFIPGLRFLPITINKRLKETGREIKTILNDIINQR 1023
            ++E+GRRIF+LQ EQAHL V++S  I+IPG RF+P   NKR+++   E+  +L  II +R
Sbjct: 214  SYEEGRRIFQLQKEQAHLAVQVSQSIYIPGWRFVPTKTNKRMRQISNEVNALLKGIIERR 273

Query: 1024 EKAMNFTELHHNDVLGLLMESNLKEMKEHGNSKNAGITLEEVIDECKLFYLAGQETVSTL 1203
            EKAM   E  ++D+LGLLMESN KEM+EHG  KN G++ ++VI+ECKLFY AGQET S L
Sbjct: 274  EKAMKVGETANDDLLGLLMESNYKEMQEHGERKNVGMSNKDVIEECKLFYFAGQETTSVL 333

Query: 1204 MVWTMVLLSMHQEWQVRAREEVLKLFGKR 1290
            ++WTMVLLS H  WQ RAREEVL++FG +
Sbjct: 334  LLWTMVLLSKHSNWQARAREEVLQVFGNK 362



 Score = 25.4 bits (54), Expect(2) = e-102
 Identities = 13/35 (37%), Positives = 23/35 (65%)
 Frame = +2

Query: 203 MREALLALVVVCSTLVISWALKVFYLIWLKPKRIE 307
           M+ + +A+     TL+I +A ++   +WLKPK+IE
Sbjct: 1   MKLSSVAISFAFITLLI-YAWRLLNSVWLKPKKIE 34


>emb|CBI39916.3| unnamed protein product [Vitis vinifera]
          Length = 505

 Score =  375 bits (963), Expect(2) = e-102
 Identities = 179/329 (54%), Positives = 245/329 (74%), Gaps = 1/329 (0%)
 Frame = +1

Query: 307  KYLRKQGIKGSPYKLLVGNFLELAKVTNEAQSHPMELSHKIVPRVVPFIYRTIEKYGKTT 486
            +YLR+QG+ G+ Y+LL G+F E++++ +EA S P+ LS  IV RV+PF Y +I+KYGK  
Sbjct: 35   RYLRQQGLIGNSYRLLHGDFREMSRMIDEANSRPISLSDDIVQRVLPFHYHSIKKYGKNC 94

Query: 487  LAWYGPTPRVVILDPEVTKDIMTSQFRDFPKPKSNSFVDFVLTGLVTYEGEQWTKHRKIL 666
              W GP P V I++PE+ +D++      F KP  +     + TG++  EGEQWTK RKI+
Sbjct: 95   FIWMGPKPVVNIMEPELIRDVLLKH-NAFQKPPVHPLGKLLATGVIALEGEQWTKRRKII 153

Query: 667  NPAFHLEKLKLMLPAFHTTCSELISKWEKQVSEK-SCEVELCHDFDKLTGDVISRTAFGS 843
            NPAFHLEKLK M+PAF  +CSE+++KWEK++S+  SCE+++  D + L GDVISRTAFGS
Sbjct: 154  NPAFHLEKLKHMVPAFQLSCSEMVNKWEKKLSKDGSCELDIWPDLENLAGDVISRTAFGS 213

Query: 844  NFEQGRRIFELQVEQAHLVVKISPYIFIPGLRFLPITINKRLKETGREIKTILNDIINQR 1023
            ++E+GRRIF+LQ EQAHL V++S  I+IPG RF+P   NKR+++   E+  +L  II +R
Sbjct: 214  SYEEGRRIFQLQKEQAHLAVQVSQSIYIPGWRFVPTKTNKRMRQISNEVNALLKGIIERR 273

Query: 1024 EKAMNFTELHHNDVLGLLMESNLKEMKEHGNSKNAGITLEEVIDECKLFYLAGQETVSTL 1203
            EKAM   E  ++D+LGLLMESN KEM+EHG  KN G++ ++VI+ECKLFY AGQET S L
Sbjct: 274  EKAMKVGETANDDLLGLLMESNYKEMQEHGERKNVGMSNKDVIEECKLFYFAGQETTSVL 333

Query: 1204 MVWTMVLLSMHQEWQVRAREEVLKLFGKR 1290
            ++WTMVLLS H  WQ RAREEVL++FG +
Sbjct: 334  LLWTMVLLSKHSNWQARAREEVLQVFGNK 362



 Score = 25.4 bits (54), Expect(2) = e-102
 Identities = 13/35 (37%), Positives = 23/35 (65%)
 Frame = +2

Query: 203 MREALLALVVVCSTLVISWALKVFYLIWLKPKRIE 307
           M+ + +A+     TL+I +A ++   +WLKPK+IE
Sbjct: 1   MKLSSVAISFAFITLLI-YAWRLLNSVWLKPKKIE 34