BLASTX nr result
ID: Coptis21_contig00023312
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00023312
(968 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004160093.1| PREDICTED: LOW QUALITY PROTEIN: DNA ligase 1... 131 5e-49
ref|XP_004145818.1| PREDICTED: DNA ligase 1-like [Cucumis sativus] 131 5e-49
ref|XP_002314303.1| predicted protein [Populus trichocarpa] gi|2... 130 1e-47
ref|XP_002272683.1| PREDICTED: DNA ligase 1 [Vitis vinifera] gi|... 127 2e-47
dbj|BAE71211.1| putative DNA ligase [Trifolium pratense] 128 1e-46
>ref|XP_004160093.1| PREDICTED: LOW QUALITY PROTEIN: DNA ligase 1-like [Cucumis sativus]
Length = 801
Score = 131 bits (330), Expect(4) = 5e-49
Identities = 68/130 (52%), Positives = 91/130 (70%)
Frame = +1
Query: 208 DSQV*KRSHIERLFKSGTNCGPQYIICLLLSKLGIDLGEQTVLVVLGQAAVYSENRPPSQ 387
DSQ K++HI+ L + T+C P Y+I LL +KL I L EQT+L LGQAAVYSE
Sbjct: 317 DSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQTLLAALGQAAVYSEKHSTPP 376
Query: 388 LHFKSSPEEVAKIVKKVCSEFPDFDRIIPTLLESGVLKLSESCRFTPCVLIGSMLSKPTK 567
+ +S EE +KIVK+V S P +D+++P LL GV LS++C FTP + IG ML+KPTK
Sbjct: 377 PNIQSPLEEASKIVKQVYSILPVYDKLVPALLSGGVWNLSKTCNFTPGIPIGPMLAKPTK 436
Query: 568 GISQILDKFQ 597
G+S+IL+KFQ
Sbjct: 437 GVSEILNKFQ 446
Score = 68.9 bits (167), Expect(4) = 5e-49
Identities = 33/56 (58%), Positives = 42/56 (75%), Gaps = 2/56 (3%)
Frame = +2
Query: 584 LISSRPGTKFTCEYKYDNEHAQIQ--KDGYVKIYSRNGDRNTGKFPGVVNSVKRLK 745
+++ G +FTCEYKYD E AQI +DG V+IYSRN +RNTGKFP VV +V R+K
Sbjct: 441 ILNKFQGLEFTCEYKYDGERAQIHYMEDGSVEIYSRNAERNTGKFPDVVLTVSRIK 496
Score = 35.4 bits (80), Expect(4) = 5e-49
Identities = 32/83 (38%), Positives = 36/83 (43%), Gaps = 16/83 (19%)
Frame = +2
Query: 2 KISEKHIKEELGIRKPSLIKV----------------KKCCDLGGFVKERCLSQLE*FNY 133
KI+ H ELGI S+IK K+ DLG K SQ
Sbjct: 236 KIAPAHEGLELGIGDASIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQS--MMR 293
Query: 134 KPAPLTVVKVLNTFPTIANESGK 202
KP LTV KV +TF IA ESGK
Sbjct: 294 KPDALTVTKVFDTFRLIAKESGK 316
Score = 26.9 bits (58), Expect(4) = 5e-49
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +3
Query: 777 AFDVKKQKIMPFQILTYKCK 836
A+D KQKI+PFQIL+ + +
Sbjct: 511 AYDRGKQKILPFQILSTRAR 530
>ref|XP_004145818.1| PREDICTED: DNA ligase 1-like [Cucumis sativus]
Length = 801
Score = 131 bits (330), Expect(4) = 5e-49
Identities = 68/130 (52%), Positives = 91/130 (70%)
Frame = +1
Query: 208 DSQV*KRSHIERLFKSGTNCGPQYIICLLLSKLGIDLGEQTVLVVLGQAAVYSENRPPSQ 387
DSQ K++HI+ L + T+C P Y+I LL +KL I L EQT+L LGQAAVYSE
Sbjct: 317 DSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQTLLAALGQAAVYSEKHSTPP 376
Query: 388 LHFKSSPEEVAKIVKKVCSEFPDFDRIIPTLLESGVLKLSESCRFTPCVLIGSMLSKPTK 567
+ +S EE +KIVK+V S P +D+++P LL GV LS++C FTP + IG ML+KPTK
Sbjct: 377 PNIQSPLEEASKIVKQVYSILPVYDKLVPALLSGGVWNLSKTCNFTPGIPIGPMLAKPTK 436
Query: 568 GISQILDKFQ 597
G+S+IL+KFQ
Sbjct: 437 GVSEILNKFQ 446
Score = 68.9 bits (167), Expect(4) = 5e-49
Identities = 33/56 (58%), Positives = 42/56 (75%), Gaps = 2/56 (3%)
Frame = +2
Query: 584 LISSRPGTKFTCEYKYDNEHAQIQ--KDGYVKIYSRNGDRNTGKFPGVVNSVKRLK 745
+++ G +FTCEYKYD E AQI +DG V+IYSRN +RNTGKFP VV +V R+K
Sbjct: 441 ILNKFQGLEFTCEYKYDGERAQIHYMEDGSVEIYSRNAERNTGKFPDVVLTVSRIK 496
Score = 35.4 bits (80), Expect(4) = 5e-49
Identities = 32/83 (38%), Positives = 36/83 (43%), Gaps = 16/83 (19%)
Frame = +2
Query: 2 KISEKHIKEELGIRKPSLIKV----------------KKCCDLGGFVKERCLSQLE*FNY 133
KI+ H ELGI S+IK K+ DLG K SQ
Sbjct: 236 KIAPAHEGLELGIGDASIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQS--MMR 293
Query: 134 KPAPLTVVKVLNTFPTIANESGK 202
KP LTV KV +TF IA ESGK
Sbjct: 294 KPDALTVTKVFDTFRLIAKESGK 316
Score = 26.9 bits (58), Expect(4) = 5e-49
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +3
Query: 777 AFDVKKQKIMPFQILTYKCK 836
A+D KQKI+PFQIL+ + +
Sbjct: 511 AYDRGKQKILPFQILSTRAR 530
>ref|XP_002314303.1| predicted protein [Populus trichocarpa] gi|222850711|gb|EEE88258.1|
predicted protein [Populus trichocarpa]
Length = 684
Score = 130 bits (328), Expect(4) = 1e-47
Identities = 68/130 (52%), Positives = 89/130 (68%)
Frame = +1
Query: 208 DSQV*KRSHIERLFKSGTNCGPQYIICLLLSKLGIDLGEQTVLVVLGQAAVYSENRPPSQ 387
DSQ K++HI+ L + T+C P Y+I LL +KL I L EQT+L LGQAAVY+E
Sbjct: 199 DSQDKKKNHIKALLVAATDCEPLYLIRLLQTKLRIGLAEQTLLAALGQAAVYTEEHSTPP 258
Query: 388 LHFKSSPEEVAKIVKKVCSEFPDFDRIIPTLLESGVLKLSESCRFTPCVLIGSMLSKPTK 567
H +S EE AKIVK+V S P +D+I+P LL GV L ++C FTP V +G ML+KPTK
Sbjct: 259 PHIQSPLEEAAKIVKQVYSVLPVYDKIVPALLSDGVWNLPKTCSFTPGVPVGPMLAKPTK 318
Query: 568 GISQILDKFQ 597
G+S+I+ KFQ
Sbjct: 319 GVSEIVTKFQ 328
Score = 62.8 bits (151), Expect(4) = 1e-47
Identities = 30/48 (62%), Positives = 37/48 (77%), Gaps = 2/48 (4%)
Frame = +2
Query: 608 KFTCEYKYDNEHAQIQ--KDGYVKIYSRNGDRNTGKFPGVVNSVKRLK 745
+F+CEYKYD E AQI ++G V+IYSRN +RNTGKFP VV + RLK
Sbjct: 331 EFSCEYKYDGERAQIHYLENGSVEIYSRNSERNTGKFPDVVAVISRLK 378
Score = 38.1 bits (87), Expect(4) = 1e-47
Identities = 31/83 (37%), Positives = 36/83 (43%), Gaps = 16/83 (19%)
Frame = +2
Query: 2 KISEKHIKEELGIRKPSLIKV----------------KKCCDLGGFVKERCLSQLE*FNY 133
K++ H ELGI + +IK K DLG K SQ
Sbjct: 118 KVAPAHEGVELGIGEALIIKALAEACGRKEKEVKKQYKDLGDLGLVAKASRSSQS--MMR 175
Query: 134 KPAPLTVVKVLNTFPTIANESGK 202
KP PLT+ KV NTF IA ESGK
Sbjct: 176 KPDPLTITKVFNTFQQIAKESGK 198
Score = 26.6 bits (57), Expect(4) = 1e-47
Identities = 10/20 (50%), Positives = 17/20 (85%)
Frame = +3
Query: 777 AFDVKKQKIMPFQILTYKCK 836
A+D +K+KI+PFQIL+ + +
Sbjct: 393 AYDREKKKILPFQILSTRAR 412
>ref|XP_002272683.1| PREDICTED: DNA ligase 1 [Vitis vinifera]
gi|297734904|emb|CBI17138.3| unnamed protein product
[Vitis vinifera]
Length = 723
Score = 127 bits (318), Expect(4) = 2e-47
Identities = 68/133 (51%), Positives = 91/133 (68%), Gaps = 3/133 (2%)
Frame = +1
Query: 208 DSQV*KRSHIERLFKSGTNCGPQYIICLLLSKLGIDLGEQTVLVVLGQAAVYSEN---RP 378
DSQ K++HI+ L + T+C PQY+I LL +KL I L EQT+L LGQAAVY++ +P
Sbjct: 238 DSQEKKKNHIKTLLVAATDCEPQYLIRLLQTKLRIGLAEQTLLTALGQAAVYNDKELTQP 297
Query: 379 PSQLHFKSSPEEVAKIVKKVCSEFPDFDRIIPTLLESGVLKLSESCRFTPCVLIGSMLSK 558
P H +S EE A IVK+ S P +D+I+P LL GV L ++C F P V +G ML+K
Sbjct: 298 PP--HIQSPLEEAANIVKQSYSVLPVYDKIVPALLSGGVWNLPKTCSFMPGVPVGPMLAK 355
Query: 559 PTKGISQILDKFQ 597
PTKG+S+I+DKFQ
Sbjct: 356 PTKGVSEIIDKFQ 368
Score = 66.6 bits (161), Expect(4) = 2e-47
Identities = 32/48 (66%), Positives = 39/48 (81%), Gaps = 2/48 (4%)
Frame = +2
Query: 608 KFTCEYKYDNEHAQI--QKDGYVKIYSRNGDRNTGKFPGVVNSVKRLK 745
+FTCEYKYD E AQI ++DG V+IYSRN +RNTGK+P VV +V RLK
Sbjct: 371 EFTCEYKYDGERAQIHYKEDGSVEIYSRNAERNTGKYPDVVVAVSRLK 418
Score = 38.9 bits (89), Expect(4) = 2e-47
Identities = 33/83 (39%), Positives = 37/83 (44%), Gaps = 16/83 (19%)
Frame = +2
Query: 2 KISEKHIKEELGIRKPSLIKV----------------KKCCDLGGFVKERCLSQLE*FNY 133
+I+ H ELGI S+IK K DLG V + C S
Sbjct: 157 RIAPAHEGLELGIGDASIIKALAEACGRKEEQIKSQYKALGDLG-LVAKACRSSQSLMR- 214
Query: 134 KPAPLTVVKVLNTFPTIANESGK 202
KP PLTV KV NTF IA ESGK
Sbjct: 215 KPDPLTVAKVFNTFGLIAKESGK 237
Score = 25.0 bits (53), Expect(4) = 2e-47
Identities = 9/20 (45%), Positives = 16/20 (80%)
Frame = +3
Query: 777 AFDVKKQKIMPFQILTYKCK 836
A+D +K KI+PFQ+L+ + +
Sbjct: 433 AYDRQKGKILPFQVLSTRAR 452
>dbj|BAE71211.1| putative DNA ligase [Trifolium pratense]
Length = 682
Score = 128 bits (321), Expect(4) = 1e-46
Identities = 71/132 (53%), Positives = 93/132 (70%), Gaps = 2/132 (1%)
Frame = +1
Query: 208 DSQV*KRSHIERLFKSGTNCGPQYIICLLLSKLGIDLGEQTVLVVLGQAAVYSE--NRPP 381
DSQ K++HI+ L + T+C P YII LL +KL I EQT+L LGQAAVY+E ++PP
Sbjct: 198 DSQEKKKNHIKSLLVAATDCEPLYIIRLLQTKLRIGYAEQTLLAALGQAAVYTEEHSKPP 257
Query: 382 SQLHFKSSPEEVAKIVKKVCSEFPDFDRIIPTLLESGVLKLSESCRFTPCVLIGSMLSKP 561
++ KS EE A+IVKKV S PD+D+I+ LL GV +L + C FTP V +G MLSK
Sbjct: 258 PEI--KSPFEEAAQIVKKVYSVLPDYDKIVAALLTDGVWELPKKCDFTPGVPVGPMLSKA 315
Query: 562 TKGISQILDKFQ 597
TKG+S+IL+KFQ
Sbjct: 316 TKGVSEILNKFQ 327
Score = 65.9 bits (159), Expect(4) = 1e-46
Identities = 32/48 (66%), Positives = 38/48 (79%), Gaps = 2/48 (4%)
Frame = +2
Query: 608 KFTCEYKYDNEHAQIQ--KDGYVKIYSRNGDRNTGKFPGVVNSVKRLK 745
+FTCEYKYD E AQI ++G V+IYSRN +RNTGKFP VV +V RLK
Sbjct: 330 EFTCEYKYDGERAQIHYLENGSVEIYSRNAERNTGKFPDVVAAVSRLK 377
Score = 35.0 bits (79), Expect(4) = 1e-46
Identities = 31/83 (37%), Positives = 36/83 (43%), Gaps = 16/83 (19%)
Frame = +2
Query: 2 KISEKHIKEELGIRKPSLIKV----------------KKCCDLGGFVKERCLSQLE*FNY 133
+I+ H ELGI S+IK K+ DLG K SQ
Sbjct: 117 RIAPAHEGLELGIGDASIIKALAEACGRTEQQIRIQYKEKGDLGLVAKASRSSQS--MMR 174
Query: 134 KPAPLTVVKVLNTFPTIANESGK 202
KP PLT+ KV TF IA ESGK
Sbjct: 175 KPEPLTIRKVFKTFHLIAKESGK 197
Score = 25.8 bits (55), Expect(4) = 1e-46
Identities = 9/20 (45%), Positives = 16/20 (80%)
Frame = +3
Query: 777 AFDVKKQKIMPFQILTYKCK 836
A+D KQ+I+PFQ+L+ + +
Sbjct: 392 AYDRAKQRILPFQVLSTRAR 411