BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00023186
         (555 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       192   4e-47
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   166   3e-39
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   164   7e-39
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       164   1e-38
gb|ACO90239.1| canadine synthase-like protein [Thalictrum flavum]     143   2e-32

>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  192 bits (487), Expect = 4e-47
 Identities = 91/99 (91%), Positives = 97/99 (97%)
 Frame = -1

Query: 543 EDIWPEPYKFMPERFMKGEDGSANNKAMERSFLPFGAGMRICAGMDLGKLQIAFTLANLV 364
           EDIWPEPYKFMPERF++GE+ SANNKAME+SFLPFGAGMRICAGMDLGKLQIAF LANLV
Sbjct: 393 EDIWPEPYKFMPERFLQGENVSANNKAMEQSFLPFGAGMRICAGMDLGKLQIAFALANLV 452

Query: 363 NAFKWSCVDEGKLPDMSEELFAVTVMKTPLEGRISTRKV 247
           NAFKWSCVDEGKLPDMSE+LFAVT+MKTPLEGRISTRKV
Sbjct: 453 NAFKWSCVDEGKLPDMSEQLFAVTLMKTPLEGRISTRKV 491


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  166 bits (419), Expect = 3e-39
 Identities = 77/98 (78%), Positives = 87/98 (88%)
 Frame = -1

Query: 543 EDIWPEPYKFMPERFMKGEDGSANNKAMERSFLPFGAGMRICAGMDLGKLQIAFTLANLV 364
           ++IW  PYKFMPERF++GEDGSA+NKAME+SFLPF AGMRICAGMDLGKLQ AF LANLV
Sbjct: 394 QNIWTNPYKFMPERFLQGEDGSASNKAMEQSFLPFSAGMRICAGMDLGKLQFAFALANLV 453

Query: 363 NAFKWSCVDEGKLPDMSEELFAVTVMKTPLEGRISTRK 250
           NAFKWSCV+EGK PDMSEEL  V +MKTPLE +I+ RK
Sbjct: 454 NAFKWSCVEEGKFPDMSEELSFVLLMKTPLEAKITARK 491


>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
           Full=(S)-tetrahydrocolumbamine oxidase
           (methylenedioxy-bridge-forming); AltName:
           Full=Cytochrome P450 719A1 gi|15822820|dbj|BAB68769.1|
           methylenedioxy bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  164 bits (416), Expect = 7e-39
 Identities = 77/99 (77%), Positives = 87/99 (87%)
 Frame = -1

Query: 543 EDIWPEPYKFMPERFMKGEDGSANNKAMERSFLPFGAGMRICAGMDLGKLQIAFTLANLV 364
           ++IWP+PYKFMPERF++GE G+A NKAME+SFLPF AGMRICAGMDLGKLQ AF LANLV
Sbjct: 393 QNIWPDPYKFMPERFLEGETGTAYNKAMEQSFLPFSAGMRICAGMDLGKLQFAFALANLV 452

Query: 363 NAFKWSCVDEGKLPDMSEELFAVTVMKTPLEGRISTRKV 247
           NAFKWSCV+EGKLPDM EEL  V +MKTPLE RI+ R V
Sbjct: 453 NAFKWSCVEEGKLPDMGEELSFVLLMKTPLEARIAGRNV 491


>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  164 bits (414), Expect = 1e-38
 Identities = 77/100 (77%), Positives = 89/100 (89%), Gaps = 1/100 (1%)
 Frame = -1

Query: 543 EDIWPEPYKFMPERFMKGEDGSANNKAMERSFLPFGAGMRICAGMDLGKLQIAFTLANLV 364
           ED+W EP KFMPER+++GEDGS  +KAME+S LPFGAGMRICAGMDLGKLQ+AF LANLV
Sbjct: 409 EDVWLEPSKFMPERYLQGEDGSVTHKAMEQSLLPFGAGMRICAGMDLGKLQVAFVLANLV 468

Query: 363 NAFKWSCVDEGKLPDMSEELFA-VTVMKTPLEGRISTRKV 247
           NAFKWSCV++GKLPDM+EELF  + +MKTPLEGRIS R V
Sbjct: 469 NAFKWSCVEDGKLPDMTEELFGLIILMKTPLEGRISPRTV 508


>gb|ACO90239.1| canadine synthase-like protein [Thalictrum flavum]
          Length = 194

 Score =  143 bits (361), Expect = 2e-32
 Identities = 66/87 (75%), Positives = 76/87 (87%), Gaps = 1/87 (1%)
 Frame = -1

Query: 540 DIWPEPYKFMPERFMKGEDGSANNKAMERSFLPFGAGMRICAGMDLGKLQIAFTLANLVN 361
           DIW EPYKF+PERFM+GEDGSA NK+ME+SFLPFGAGMRIC GM+L KLQ+AF LANLVN
Sbjct: 108 DIWTEPYKFIPERFMQGEDGSATNKSMEKSFLPFGAGMRICGGMNLAKLQVAFILANLVN 167

Query: 360 AFKWSCVDEGKLPDMSEEL-FAVTVMK 283
            FKWSCV+EGK PDMSE+L   + +MK
Sbjct: 168 EFKWSCVEEGKFPDMSEDLPIGILLMK 194