BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00022180
         (1142 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284931.1| PREDICTED: gibberellin 3-beta-dioxygenase 4-...   310   3e-82
ref|XP_002324836.1| 2-oxoglutarate-dependent dioxygenase [Populu...   303   4e-80
ref|XP_002309592.1| 2-oxoglutarate-dependent dioxygenase [Populu...   298   2e-78
ref|XP_003526431.1| PREDICTED: LOW QUALITY PROTEIN: gibberellin ...   264   3e-68
ref|XP_002515597.1| Gibberellin 2-beta-dioxygenase, putative [Ri...   264   3e-68

>ref|XP_002284931.1| PREDICTED: gibberellin 3-beta-dioxygenase 4-like [Vitis vinifera]
          Length = 316

 Score =  310 bits (795), Expect = 3e-82
 Identities = 151/308 (49%), Positives = 207/308 (67%)
 Frame = -1

Query: 1082 REIPFLDLTKDPEDLEEGSDGWKCLSKKVREACEEYGCFQVKYNKLPTHLGEEMFKASKE 903
            +EIPFL+   D  DLE+ S+ WK L  KVREACE YGCF +  + +P  L +EMF   K 
Sbjct: 8    QEIPFLEFPGDDLDLEQESEEWKGLRSKVREACETYGCFGLVCDAIPAKLRQEMFMVMKT 67

Query: 902  LFDLPDEIKVKNLNSKPYYGYIGKIDAVPLYESLGIEDATTVDGARTFAQQIWPDGNPVF 723
            LFDLP+E K K+ ++KPY+ Y GK   VPL ES GI+DA     A+ F   +WP+GNP F
Sbjct: 68   LFDLPEETKKKHSSTKPYHSYQGKCPIVPLLESFGIDDAPQPQAAQAFTNLLWPEGNPAF 127

Query: 722  CETVNSISNKLYDLELLILKMVMDSYGAEKCCNSFAECNYTLFRLMKYSTPQSNAVEMAL 543
            C  + S++ K+ +L  ++LK++++S G EK  NS  E + ++FRLMKY+ P SN   + L
Sbjct: 128  CGALISMATKMLELNFIVLKLILESLGMEKYYNSQKENSASVFRLMKYTVPPSNDSAIGL 187

Query: 542  QPHVDKSTLTVLCQDSVQGLEVLSKEGEWLGVAPLPGVFIVIVGEALKAWSNARLHPAKH 363
              H DK+ LT+LCQ+ VQGLEVLSKEG W+ +      FIVI+G+ LKAW+N RLH  +H
Sbjct: 188  VTHTDKNILTILCQNEVQGLEVLSKEGNWIRLNFCKDSFIVIIGDLLKAWTNGRLHAVRH 247

Query: 362  RVMMDGDRPRYSFGLFLIPXXXXXXXXXXXXVDKEHPLLYRPFNYSDFLNYCTSNTLYEN 183
            RV+M+GD+ RYS G+F++P            VD +HPL YRPF YSD++++ TSN + ++
Sbjct: 248  RVVMNGDKDRYSCGMFVLPGDTVTIEVPEELVDNDHPLRYRPFTYSDYMSFFTSN-ICDD 306

Query: 182  VLEVYAGV 159
             LEVYAGV
Sbjct: 307  ALEVYAGV 314


>ref|XP_002324836.1| 2-oxoglutarate-dependent dioxygenase [Populus trichocarpa]
            gi|222866270|gb|EEF03401.1| 2-oxoglutarate-dependent
            dioxygenase [Populus trichocarpa]
          Length = 313

 Score =  303 bits (777), Expect = 4e-80
 Identities = 147/314 (46%), Positives = 209/314 (66%), Gaps = 2/314 (0%)
 Frame = -1

Query: 1094 MKVEREIPFLDLTKDP-EDLEEGSDGWKCLSKKVREACEEYGCFQVKYNKLPTHLGEEMF 918
            M  + EIPFLD++++    +E  S+ WK L  +VREACE +G F + Y+K+PT L EEMF
Sbjct: 1    MAAKVEIPFLDISEEALAGIEVKSEKWKELCNQVREACETHGIFFLVYDKIPTSLREEMF 60

Query: 917  KASKELFDLPDEIKVKNLNSKPYYGYIGKIDAVPLYESLGIEDATTVDGARTFAQQIWPD 738
             A K LFDLP+E K K++N KPY  Y+G    +P +ES G++DA T+D ++ F   +WP+
Sbjct: 61   VALKALFDLPEETKNKHVNPKPYRSYLGNCPVIPFHESFGVDDAPTLDASQAFTNLMWPE 120

Query: 737  GNPVFCETVNSISNKLYDLELLILKMVMDSYGAEKCCNSFAECNYTLFRLMKYSTPQS-N 561
            GNP FCET++ +S+K+ +L  L++KM+ +S+  EK  +SF E   ++FR+MKY  P S  
Sbjct: 121  GNPSFCETIHGMSSKMQELNFLVMKMIYESFDIEKLYDSFVEETTSIFRIMKYKVPPSAT 180

Query: 560  AVEMALQPHVDKSTLTVLCQDSVQGLEVLSKEGEWLGVAPLPGVFIVIVGEALKAWSNAR 381
               + L  H DK+ +T+LCQ+ VQGLE+ +K G W  V      F  +VG+ALKAWSN R
Sbjct: 181  DSSIGLVAHTDKNAITILCQNEVQGLEIQTKNGYWAQVMVPENAFTAVVGDALKAWSNGR 240

Query: 380  LHPAKHRVMMDGDRPRYSFGLFLIPXXXXXXXXXXXXVDKEHPLLYRPFNYSDFLNYCTS 201
            LH A+HRV++ G++ RYS GLF +P            VDKEHPL YRPFNYSD+++Y  S
Sbjct: 241  LHAARHRVVISGEKDRYSCGLFSMPKEEAVVEVPDELVDKEHPLQYRPFNYSDYISYFVS 300

Query: 200  NTLYENVLEVYAGV 159
              L ++ LE+YAG+
Sbjct: 301  K-LSDDALEIYAGI 313


>ref|XP_002309592.1| 2-oxoglutarate-dependent dioxygenase [Populus trichocarpa]
            gi|222855568|gb|EEE93115.1| 2-oxoglutarate-dependent
            dioxygenase [Populus trichocarpa]
          Length = 314

 Score =  298 bits (763), Expect = 2e-78
 Identities = 149/317 (47%), Positives = 209/317 (65%), Gaps = 2/317 (0%)
 Frame = -1

Query: 1103 MDIMKVEREIPFLDLTKDP-EDLEEGSDGWKCLSKKVREACEEYGCFQVKYNKLPTHLGE 927
            M + KVE  IP LD +++    LE  S+ WK L  +VREACE +G F + Y+K+P  L E
Sbjct: 1    MAVAKVE--IPVLDFSEEALTGLEVKSEKWKELCNQVREACETHGVFFLVYDKIPGSLRE 58

Query: 926  EMFKASKELFDLPDEIKVKNLNSKPYYGYIGKIDAVPLYESLGIEDATTVDGARTFAQQI 747
            EMF A K LFDLP+E K +++N KPY  Y+GK   +P +ES G++DA T+D ++ F   +
Sbjct: 59   EMFGAMKALFDLPEETKNRHVNPKPYRSYLGKCPVIPFHESFGVDDAPTLDASQAFTTLM 118

Query: 746  WPDGNPVFCETVNSISNKLYDLELLILKMVMDSYGAEKCCNSFAECNYTLFRLMKYSTPQ 567
            WP+GNP FCET++S+S+K+ +L  L++KM+ +S+G EK  +SF E   ++ ++MKY  P 
Sbjct: 119  WPEGNPSFCETIHSMSSKMQELNFLVMKMIYESFGIEKLYDSFLEETTSILKVMKYKVPP 178

Query: 566  SNAVE-MALQPHVDKSTLTVLCQDSVQGLEVLSKEGEWLGVAPLPGVFIVIVGEALKAWS 390
            S+    + L  H DK+ +T+LCQ+ VQGLEV +K G+W  V      F  IVG+ +KAWS
Sbjct: 179  SDTESAIGLVAHTDKNAITILCQNEVQGLEVQTKNGDWAQVMVPENAFTAIVGDTVKAWS 238

Query: 389  NARLHPAKHRVMMDGDRPRYSFGLFLIPXXXXXXXXXXXXVDKEHPLLYRPFNYSDFLNY 210
            N RLH A+HRV++ GDR RYS GLF  P            VDKEHPL YRPFN+SD+L+Y
Sbjct: 239  NGRLHAARHRVVISGDRDRYSCGLFSTPKEEAVIEVPNELVDKEHPLQYRPFNFSDYLSY 298

Query: 209  CTSNTLYENVLEVYAGV 159
              S  L ++ LE+YA +
Sbjct: 299  FVSK-LSDDALEIYASI 314


>ref|XP_003526431.1| PREDICTED: LOW QUALITY PROTEIN: gibberellin 20 oxidase 1-like
            [Glycine max]
          Length = 313

 Score =  264 bits (675), Expect = 3e-68
 Identities = 146/317 (46%), Positives = 197/317 (62%), Gaps = 5/317 (1%)
 Frame = -1

Query: 1094 MKVEREIPFLDLTKDPEDLEEGSDGWKCLSKKVREACEEYGCFQVKYNKL-PTHLGEEMF 918
            M+ EREI  +      + LEEGS+ WK +SKKVREACE +GCF +  +++ P  + EE F
Sbjct: 1    MESEREIMMIPCFDFGKALEEGSEEWKEMSKKVREACESHGCFLLVCDEMIPKGVREEFF 60

Query: 917  KASKELFDLPDEIKVKNLNSKPYYGYIGKIDAVPLYESLGIEDATTVDGARTFAQQIWPD 738
               + LFDLP+E K+K+++ KPY  Y GK   +PL E+ GI+D      A  F   +WP 
Sbjct: 61   SNMEALFDLPEERKMKHISPKPYSSYSGKSPVIPLSETFGIDDVPLSASAEAFTNLMWPQ 120

Query: 737  GNPVFCETVNSISNKLYDLELLILKMVMDSYGAEKCCNSFAE--CNYTLFRLMKYSTPQ- 567
            GNP FCET+  +S+K+  L  LILKM+++ YG ++   S  E   + +  RL+KY  P+ 
Sbjct: 121  GNPPFCETLKIMSSKMLKLSSLILKMIVEDYGIQQHYISDVEKMKSSSNSRLIKYKIPEN 180

Query: 566  SNAVEMALQPHVDKSTLTVLCQDSVQGLEVLSKEGEWLGVAPLPGVFIVIVGEALKAWSN 387
            +N     L  H DK+ LT++CQ+ VQGL+VLSK   W+    L   F+VIVG+ LKAWSN
Sbjct: 181  NNDSNTGLVSHTDKNALTIICQNEVQGLQVLSKTDNWI---ELXDGFVVIVGDILKAWSN 237

Query: 386  ARLHPAKHRVMMDGDRPRYSFGLFLIPXXXXXXXXXXXXVD-KEHPLLYRPFNYSDFLNY 210
             RLH A HRVMM GD+ RYSFGLF +P            VD K HPL Y PF Y ++++Y
Sbjct: 238  GRLHAATHRVMMSGDKERYSFGLFTMPKEEMDIEVPIELVDEKIHPLRYHPFKYGEYISY 297

Query: 209  CTSNTLYENVLEVYAGV 159
              SN L EN LEV+AG+
Sbjct: 298  FVSN-LKENALEVFAGL 313


>ref|XP_002515597.1| Gibberellin 2-beta-dioxygenase, putative [Ricinus communis]
            gi|223545541|gb|EEF47046.1| Gibberellin
            2-beta-dioxygenase, putative [Ricinus communis]
          Length = 316

 Score =  264 bits (675), Expect = 3e-68
 Identities = 136/315 (43%), Positives = 198/315 (62%), Gaps = 8/315 (2%)
 Frame = -1

Query: 1079 EIPFLDLTKDPEDLEEGSDGWKCLSKKVREACEEYGCFQVKYN--KLPTHLGEEMFKASK 906
            EIP L  +   E L  GS+  + L  +VR+ACE +GCF + Y+   +P  L + +F + K
Sbjct: 5    EIPTLYFSG--EALSSGSESRRELCNRVRKACESHGCFLLMYDHGNIPAKLRQNLFVSMK 62

Query: 905  ELFDLPDEIKVKNLNSKPYYGYIGKIDAVPLYESLGIEDATTVDGARTFAQQIWPDGNPV 726
             LFDLP++ K K +N KPY  Y G+   +P YES G++DA  +   + F   +WP GN  
Sbjct: 63   ALFDLPEDTKTKYVNPKPYRSYTGRSPVIPFYESFGLDDAHKLGQVQDFTNLMWPQGNTS 122

Query: 725  FCETVNSISNKLYDLELLILKMVMDSYGAEKCCNSFAECNYTLFRLMKYSTPQSNA---- 558
            FCE ++ +S+K+ ++ L++LKM+ +S+G EK  +S  + + +LF++MKY  P SN     
Sbjct: 123  FCEILHVMSSKMLEMNLMVLKMIFESFGMEKHYDSHIQDSTSLFKVMKYIAPPSNGRGSG 182

Query: 557  --VEMALQPHVDKSTLTVLCQDSVQGLEVLSKEGEWLGVAPLPGVFIVIVGEALKAWSNA 384
                +AL+ H DK+ +T+LCQ+ VQGLEV SK+GEW  V      F+VIVGEALK WSN 
Sbjct: 183  EDSAIALRAHRDKNAITILCQNEVQGLEVESKDGEWARVMVPQDAFVVIVGEALKVWSNG 242

Query: 383  RLHPAKHRVMMDGDRPRYSFGLFLIPXXXXXXXXXXXXVDKEHPLLYRPFNYSDFLNYCT 204
            RL  A+HRV+++G   RYS  LF +P            VDK+HPLLYRPF +SD+++Y  
Sbjct: 243  RLEAARHRVVINGKEDRYSCALFSMPKEESKIEVPCELVDKDHPLLYRPFIFSDYISYFV 302

Query: 203  SNTLYENVLEVYAGV 159
            S  L ++ LE+YAG+
Sbjct: 303  SK-LSDDALEIYAGI 316